중온 침출수 재순환 혐기성 소화 시스템을 이용한 음식물류 폐기물 처리

조찬휘ㆍ이병희ㆍ이용운(Chan Hui ChoㆍByong hi LeeㆍYong Woon Lee) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

본 연구에서는 침출수 재순환 시스템을 적용한 중온 혐기성소화를 이용하여 음식물류 폐기물을 분해하여 메탄가스를 생산하였다. 실험은 36℃로 유지되는 항온수조 내에 생물반응조와 침출수 저장조로 구성된 2개의 동일한 시스템(System A, System B)을 사용하였고, 생물반응조 하단 30 mm위에는 스크린이 있어 고액분리를 하여 침출수 저장조로 침출수를 이송하였다. 침출수 재순환은 매일 수행하였으며, 침출수 재순환 시에는 생물반응조 하단에서 침출수 저장조로 2.5 L를 30분간 이송한 뒤 다시 침출수 저장조에서 생물반응조 상부로 2.5 L를 30분간 주입하였다. 주입된 음식물류 폐기물은 수집되기 전 한 번 세척하였으며 반응조에 주입되기 전에 36℃로 온도를 올렸다. System A에 49.1 g VS, System B에 54.0 g VS을 2주 간격으로 투입하였다. 저해인자로 측정된 항목은 NH₄⁺-N과 염도였으며, 두 가지 항목의 농도 모두 시스템에 끼친 영향은 없는 것으로 나타났다. System A는 112일간, System B는 140일 동안 운전하였는데, 각 시스템에서 인발된 슬러지는 없었다. 음식물류 폐기물의 혐기성 소화를 통한 평균 메탄 발생량은 System A의 경우 0.439 L CH4/g VS, System B의 경우 0.368 L CH4/g VS로 나타났다. In this study, mesophilic anaerobic digestion of source separated food waste was carried out by leachate recirculation system and methane gas was produced. Two systems - system A and B were fabricated and placed within water bath to maintain 36℃. Each system was comprised of an anaerobic bioreactor and a leachate tank. Leachate in bioreactor was separated through the screen located at 30 mm above the bottom and a pump was installed to transfer collected leachate to the leachate tank. Everyday, 2.5 L of the leachate was pumped from the bioreactor to the leachate tank for 30 min and transferred leachate was pumped back to the top of the bioreactor for 30min, sequentially. Source separated food waste used for this experiment was washed by water before transferring to the laboratory. Transferred food waste was warmed to 36℃ before being fed to bioreactors. System A was fed to 49.1 g VS (Volatile Solids) and System B was fed to 54.0 g VS at every two weeks, respectively. NH₄⁺-N and salinity were monitored to see the inhibition toward anaerobic bioreaction and it was found that concentrations of these materials were not high enough to affect the bioreaction. Although the food waste was fed biweekly for 112 days and 140 days at system A and B, respectively, there was no sludge withdrawal from each system. Average methane productions rates were 0.439 L CH4/g VS and 0.368 L CH4/g VS for system A and B, respectively.

고속도강을 이용한 CFRP 복합재료의 드릴가공 특성

조규재,박종남,정용운 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.1

복합재료는 각 종 기계부품, 우주항공산업, 자동차의 내장재와 구조재, 건축구조물, 선박 재료, 스포츠용품 등 다방면에서 유용하게 사용되어지고 있다. 복합재료는 여러 가지의 대체 재료로서 타 산업에 비하여 활용의 가치가 높다. 그러나 기계가공분야에서는 가공의 어려움으로 인하여 활용도가 미흡한 상태이다. 따라서 본 논문에서는 CFRP를 수직형 머시닝센터에서 고속도강 드렬로 가공한 후 흘 가공 입 · 출구부에서 표면이 고르지 못함을 확인하였으며, 가공을 할수록 유동형의 긴 칩에서 분진형태로 변하는 칩의 형상 등을 관찰하였다. Composite materials are widely used in all kinds of machine parts, aerospace industries, internal and structural materials of cars, building structures, ship materials, sporting goods, and others. It is worth while to use the composite materials as various substitutions compared with others. But it is limited to use them in the field of the mechanical processing because of the difficulties in cutting. Thus it is proved that the surface roughness is in and out of the hole processing after cutting the CFRP with HSS drilling in the vertical machining center. And it is observed that the more it is processed, the more the fluid long chip is changed into the powdered chip.

상호텍스트성 중심의 동시 교육 방법 연구

조용훈,고지운 淸州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.12 No.-

국제유도의 스포츠화 과정에 관한 고찰

조용철,이운형 龍仁大學校 武道硏究所 2001 武道硏究所誌 Vol.12 No.1

Under the realization that Judo gets more recognized as an international sport, this study, this study is aimed to take a close look at the Judo's development process to an international sport and changes resulting from the development. The study has come to the following four conclusions. First, along with Judo's transition into an international sports, the International Judo Federation (IJF) is also changing from a Japan-centered organization to a more international body. Judo has positioned itself as an international sport with the help of the IJF establishment, the regular holding of international Judo contests and the Judo's selection as an official Olympic sport event. Secondly, Judo's free practice style indicates that it still preserves martial art aspects, but the possibility of getting injured becomes minimized. In other words, it took one step further toward an international sport. Since the foundation of the IJF, Judo's techniques have been globally standardized. Thirdly, Judo games are shifting from defense-oriented to offense-oriented as game rules are in favor of offensive players over defensive ones. Fourthly, mindful of the general perception that it would be better to expand score zones, enable clear and far judgment, and turn a Judo match to a more offense-oriented one, the IJF has exerted its best efforts go make the Judo game rules more rational, realistic and simpler. It has yielded fruitful results. As mentioned above, Judo is a sport, which developed from a martial art. In the process of transition, however, it might lose its unique martial art features, which are commonly shared by oriental sports, Without martial art aspects, Judo would bo no different from other western sports. Therefore, in future studies, I would like to focus on problems looming with respect to the Judo's transformation into an international sport, and present solutions.

Osteotome Sinus Floor Elevation Procedure를 이용한 임프란트 매식

송종운,조용기,박홍주,김영운,오희균,유선열 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Maxillary sinus has an anatomic limitation to bone support available for dental implant. When there is less than 10㎜ of bone remaining between the upper alveolar ridge crest and the maxillary sinus floor, bone augmentation should be considered. In 1994, Summers reported two sinus floor elevation techniques of limmediate implant insertion, using osteotomes, for patients who have at least 5㎜ of bone remaining between the alveolar ridge crest and the maxillary sinus floor. One technique is osteotome sinus floor elevation (OSFE) procedure in which uses osteotomes to elevate the sinus floor. The other is bone-added OSFE (BAOSFE) procedure in which bone graft is added into osteotomy site. Both procedures conserve all of the remaining bone and selectively displace it upward, thereby raising sinus floor. This study was aimed to evaluate the long-term success rate of implants which had been placed by osteotome sinus floor elevation procedure. Between February 1996 and June 1998, 10 implants were placed using OSFE or BAOSFE procedures in 6 patients who had at least 6㎜ of bone remaining between the alveolar ridge crest and the sinus floor. OSFE procedures were performed in patiens, 2∼4㎜ sinus floor elevation was needed, whereas BAOSFE procedures were done in patines, more than 4㎜ sinus floor elevation was needed. During the mean follow-up period of 4 years 4 months (3 years 6 months ∼ 5 years 9 months), no implant was failed and all patients showed good functional results. OSFE and BAOSFE procedures seemed technically easier and less invasive compared to conventional sinus lift procedure. And both procedure have good success rate.

불소 국소도포가 복합레진 수복물의 접착강도 및 표면구조에 미치는 영향에 관한 비교연구

김용기,조운행 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.1

The purpose of this in vitro study were to evaluate the possible influences of topical fluoride application on bond strength and surface texture of composite resin restoration. Universal testing machine(Model No.4204, Instron) was used to measure the shear bond strength and surface changes were investigated through scanning electron microscope(JSM-5200. JEOL. JAPEN). The summary of results obtained from the present experiment were as follows: 1. The bond strengths of all groups decreased with time. 2. The mean bond strength of group Ⅱ was significantly less than those of group Ⅰ and Ⅲ(p<.05). 3. A majority of bond failure occurred at tooth-resin interface of samples. 4. APF produced a negative effect on resin surface with or without protecting agent application. whereas NaF did not. 5. The resin glaze treatment provided superior surface protecting effect microscopically to bonding resin treatment within the present experimental period. 6. The results of this study indicate that the resin surface should be covered with protecting agent prior to APF topical application and that APF should not be applied before acid etching when the bond strength of resin is considered.

Escherichia coli(M15)을 이용한 serotonin N-acetyltransferase 유전자의 발현

김용만,정미영,윤경식,진병관,백행운,조용호,백형환 慶熙大學校 1997 論文集 Vol.26 No.-

AA-NAT cDNA was obtained by RT-PCR technique from total RNA of rat sacrified at night(02:00am). pCRNAT was cloned using the pCRII vector with insertion of AA-NAT cDNA(about 1.4 kb) at EcoRI site. For the expression of the gene, pQECNAT was subcloned, in which the coding region of AA-NAT was inserted into expression vector pQE30 at BamHI and HindIII sites. According to the experimental results, Escherichia coli strain M15, transformed by the expession vector pQECNAT, was selected as a host to express AA-NAT gene with the induction of isopropyl β-D-thiogalactoside(IPTG). Optimal condition for the expression of AA-NAT gene was achieve from the experimental results, showing that the expression of the protein was inducible much 19.800 ± 2,200 dpm per ml of culture volume in 4 hours at the concentration of 2 mM IPTG. Partial purification through the affinity column(Ni-NTA agarose) binding to the continuous 6 histidine residues of protein resulted in 5 times more increase in the specific activity of AA-NAT than that of the homogenate of bacterial pellet. These experimental results will provide basic data in further study for the enzymatic kinetics and antibody production of AA-NAT.

구속조건과 온도관리가 콘크리트 벽체의 균열 거동에 미치는 영향

변근주,조호진,송하원,박용운 연세대학교 산업기술연구소 1996 논문집 Vol.28 No.2

Mass concrete wall with external restraint has high possibility of thermal cracking due to thermal stress caused by heat of hydration. Specially the cracking control is the most important factor for the requirements of watertightness and durability of concrete structures. In this study, the results by finite element thermal analysis on concrete wall are compared with experimental data and verified, and then various parameters which affect thermal cracking of the wall are analyzed using the program. The parameters include cement and fly-ash contents, concrete placement volume, concrete placement temperature, and ambient temperature during concrete placement and curing. From the study it is concluded that pre-cooling method or the use of crushed ice which lower placement temperature down to 10℃ is the most effective way to control the thermal cracking of massive concrete walls.

운동부하 흰쥐에서 포도당 증합체가 심근 당원에 미치는 영향

이석강,조성현,김용운,박덕일,김종연,장용찬 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.1

운동부하전 섭취한 탄수화물이 운동흰쥐의 심근 및 골격근이 당원량에 미치는 영향을 측정하여 심근과 골격근의 대사적 차이점의 일단을 연구한 본 논문의 결과는 다음과 같다. 심근과 골격근 당원량(mg/ g wet tissue, 이하 단위 동일)은 정상군에서 심근 2.62±0.51로서 골격근의 3.37±0.58보다 낮았다(p<0.01). 심근 당원량은 정상군이 2.62±0.51이었다. 운동 30분에서 대조군은 정상군의 63%수준으로 감소하였으며(p<0.01), 탄수화물섭취군은 정상군의 86 및 90%수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 대조군의 142 및 137%정도로 양군 모두 대조군보다 유의하게(p<0.01) 높았다. 운동 60분에는 대조군은 운동30분의 78%(정상군의 49%)수준으로 감소하였으며(p<0.05) 탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 운동 30분에 비하여 72(p<0.01, 정상군의 62%), 81%정도(p<0.05, 정상군의 73%)로 감소하였다. 포도당 섭취군의 당원량은 대조군의 147% 정도로 대조군보다 유의하게(p<0.01) 높았다. 운동 120분에서 대조군은 운동 60분에 비하여 63%(정상군의 33%) 수준으로 감소하였으며 (p<0.01) 포도당섭취군은 운동 60분의 69%(정상군의 50%)수준으로 유의하게 감소하였으나, 포도당 중합체 섭취군은 차이가 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 202 및 162% 정도로 양군 모두 대조군보다 유의하게(p<0.01)높았다. 골격근 당원량은 정상군이 3.37±0.58 이었다. 운동 30분에서 대조군은 정상군의 69% 수준으로 감소하였으며(p<0.01) 탄수화물섭취군은 포도당 중합체 및 포도당섭취군에서 각각 정상군의 85 및 88% 수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 125 및 129% 정도로 양군 모두 대조군보다 유의하게(p<0.05) 높았다. 운동 60분에는 대조군은 운동 30분의 77%(정상군의 53%) 수준으로 감소하였으며(p<0.05)탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 30분에 비하여 93(정상군의 80%), 89(정상군의 78%) %정도로 감소하였으나 통계적으로 유의하지는 않았다. 포도당중합체 및 포도당 섭취군의 당원량은 각각 대조군의 152 및 149% 정도로 대조군보다 유의하게 (p<0.001) 많았다. 운동 120분에서 대조군은 운동 60분에 비하여 50%(정상군의 26%) 수준으로 감소하였으며(p<0.001) 포도당 섭취군은 운동 60분의 75%(정상군의 58%)수준으로 유의하게(p<0.05) 감소하였으나 포도당 중합체 섭취군은 대조군의 84%(정상군의 67%)정도로 감소하는 경향을 보였다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군우ㅢ 255 및 221% 정도로 양군 모두 대조군보다 유의하게(p<0.001) 높았다. 이상의 결과로 미루어 보아 심근과 골격근 간의 생리, 생화학적 특성의 차이에도 불구하고 탄수화물의 섭취후 중등도 정도의 달리기 운동시 근 당원의 함량에 미치는 영향은 정도의 차이는 있으나 같은 경향을 나타내었으며, 골격근에서 심근보다 더 많은 근 당원 절감효과를 나타내었다. 또한 운동전 탄수화물의 섭취시 이러한 근 당원 절감 효과로 장시간의 운동시 심근 및 골격근 당원의 고갈 지연으로 운동수행능력의 증가가 기대되며, 특히 포도당 보다는 포도당 중합체의 섭취시 효과가 더 크리라고 사료된다. The purpose of this study was to investigate the effect of glucose and glucose plymer ingestion on the concentration of the cardiac muscle glycogen at 30, 60 and 12 minutes of the treadmill running in rats. Rats were randomly assinged to normal, control, and carbohydrate groups. Carbohydrate group was divided into glucose, and glucose polymer ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either water (control) of carbohydrates of formulated glucose(6%), and glucose polymer(6%) via polyethylene tube to the stomaach before treadmill run. Exercise was loaded by a treadmill run at the speed of I ㎞/hr for 30, 60, and 120 minutes. The results of experiment were as follows: Glycogen content of the cardiac muscle was lower than that of the skeletal muscle (plantaris) in normal rats. Glycogen content of the cardiac muscle was decreased in proportion to the exercise time compared to normal in control and carbobydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in decreasing order at 120 minutes treadmill running (% change of glycogen content of control, glucose, and glucose polymer compared to normal rats were 33, 50, and 63% in the cardiac muscle at 120 minutes of treadmill running respectively). Glycogen content of the skeletal muscle was decreased in proportion to the exercise time compared to normal in control and corbohydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in ecreasing order 120 minutes treadmill running(% change of glycogen content of control, glucose, and glucose polymer compared to normal were 26, 58, and 67% in the cardiac muscle at 120 minutes of treadmill running respectively). Change of the glycogen content of the cardiac and the skeletal muscle during treadmill running after glucose and glucose polymer ingestion showed that both muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrates ingested rats. Decreasing rate of glycogen content of cardiac and skeletal muscles during treadmill running compared to normal in carbohydrates ingested rats was lower than that of control. From these results, it is suggested glycogen content of cardiac and skeletal muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrate ingested rats. Glycogen content of cardiac and skeletal muscles in carbohydrate singested rats less decreased than that of control during treadmill running compared to normal. Glucose polymer of the carbohydrates ingested rats in considered as the most effective for glycogen sparing according to exercise time in both muscles. Therefore, intake of carbohydrate, especially glucose polymer, before exercise may be increase performance of the prolonged moderate exercise.

망막아세포종 세포주(Y-79)의 Arylalkylamine N-acetyltransferase 활성에 미치는 Dopamine의 영향

김석민,정미영,윤경식,진병관,백행운,백형환,조용호 慶熙大學校 1997 論文集 Vol.26 No.-

The arylalkylamine N-acetyltransferase(AA-NAT, EC 2.3.1.87) in vertebral pineal gland and retina is rate-limiting enzyme in the pathway of biosynthesis of melatonin. AA-NAT activities are remarkably increased during night with 10-150 folds. Neurohormone melatonin is thought to play roles in a broad range of physiological functions in human, primarily through effects on the biological clock in the suprachiasmatic nucleus of the hypothalamus, This rhythm reflects large changes in the activities of AA-NAT. So the important regulatory role of AA-NAT has made it central interest in research on melatonin biochemistry and neurochemical signal transduction. The neurotransmitter that regulates the AA-NAT activities is norepinephrine, which acts through a cyclic AMP mechanism. The effect of dopamine on AA-NAT in human retinoblastoma Y-79 cells was studied. the obtained results are as follows; AA-NAT activities in Y-79 cells were increased by cyclic AMP, and dopamine inhibited the AA-NAT activities stimulated by forskolin. The pattern of inhibition of dopamine on the AA-NAT activities in Y-79 cells was time and concentration dependent. The results suggest that dopamine participates in the regulation of the AA-NAT activities through the inhibitory mechanism in the human pineal and retinal tissues.