MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

High-precision Position Tracking Control of Electro-hydraulic Servo Systems Based on an Improved Structure and Desired Compensation

Wei Shen,Xinyu Liu,Xiaoyu Su 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.11

How to improve the position tracking accuracy of electro-hydraulic servo system is a hot issue today. Full state feedback control has received widespread attention for its ability to significantly improve control performance,however, its practical application range is limited in view of the large influence of measurement noise. In terms of this issue, we propose an adaptive robust controller based on improved structure and desired compensation. Firstly,to reduce the impact of measurement noise, the actual state value is substituted by the corresponding desired value in the controller design based on model compensation and the adaptive model compensator. Then, we introducea new auxiliary variable into the controller to optimize its structure. In addition, nonlinear robust control laws are integrated in the controller to balance unstructured uncertainties. Simulation analysis shows that the proposedcontrol strategy not only achieves the asymptotic tracking when parameter perturbation exists, but also ensures a specified transient response and final tracing precision under the combined influence of structured and unstructureduncertainties. The results indicate that the control strategy has good control accuracy as well as strong robustness

Thermal Management Enables Stable Perovskite Nanocrystal Light-Emitting Diodes with Novel Hole Transport Material

석경은,( Xinyu Shen ),이보람 한국공업화학회 2023 한국공업화학회 연구논문 초록집 Vol.2023 No.1

Copper-induced injectable hydrogel with nitric oxide for enhanced immunotherapy by amplifying immunogenic cell death and regulating cancer associated fibroblasts

Shuilin Shen,Zimeng Zhang,Haixiao Huang,Jing Yang,Xinyue Tao,Zhengjie Meng,Hao Ren,Xueming Li 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Immunogenic cell death (ICD) induced by different cancer treatments has been widely evaluated to recruit immune cells and trigger the specific antitumor immunity. However, cancer associated fibroblasts (CAFs) can hinder the invasion of immune cells and polarize the recruited monocytes to M2-type macrophages, which greatly restrict the efficacy of immunotherapy (IT). Methods In this study, an injectable hydrogel induced by copper (Cu) has been designed to contain antibody of PD-L1 and nitric oxide (NO) donor. The therapeutic efficacy of hydrogel was studied in 4T1 cells and CAFs in vitro and 4T1 tumor-bearing mice in vivo. The immune effects on cytotoxic T lymphocytes, dendritic cells (DCs) and macrophages were analyzed by flow cytometry. Enzyme-linked immunosorbent assay, immunofluorescence and transcriptome analyses were also performed to evaluate the underlying mechanism. Results Due to the absorbance of Cu with the near-infrared laser irradiation, the injectable hydrogel exhibits persistent photothermal effect to kill cancer cells. In addition, the Cu of hydrogel shows the Fenton-like reaction to produce reactive oxygen species as chemodynamic therapy, thereby enhancing cancer treatment and amplifying ICD. More interestingly, we have found that the released NO can significantly increase depletion of CAFs and reduce the proportion of M2-type macrophages in vitro. Furthermore, due to the amplify of ICD, injectable hydrogel can effectively increase the infiltration of immune cells and reverse the immunosuppressive tumor microenvironment (TME) by regulating CAFs to enhance the therapeutic efficacy of anti-PD-L1 in vivo. Conclusions The ion induced self-assembled hydrogel with NO could enhance immunotherapy via amplifying ICD and regulating CAFs. It provides a novel strategy to provoke a robust antitumor immune response for clinical cancer immunotherapy.

A data corruption detection scheme based on ciphertexts in cloud environment

( Sixu Guo ),( Shen He ),( Li Su ),( Xinyue Zhang ),( Huizheng Geng ),( Yang Sun ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.9

With the advent of the data era, people pay much more attention to data corruption. Aiming at the problem that the majority of existing schemes do not support corruption detection of ciphertext data stored in cloud environment, this paper proposes a data corruption detection scheme based on ciphertexts in cloud environment (DCDC). The scheme is based on the anomaly detection method of Gaussian model. Combined with related statistics knowledge and cryptography knowledge, the encrypted detection index for data corruption and corruption detection threshold for each type of data are constructed in the scheme according to the data labels; moreover, the detection token for data corruption is generated for the data to be detected according to the data labels, and the corruption detection of ciphertext data in cloud storage is realized through corresponding tokens. Security analysis shows that the algorithms in the scheme are semantically secure. Efficiency analysis and simulation results reveal that the scheme shows low computational cost and good application prospect.

Interval Type-2 Fuzzy Logic PID Controller Based on Differential Evolution with Better and Nearest Option for Hydraulic Serial Elastic Actuator

Haozhen Dong,Xinyu Li,Pi Shen,Liang Gao,Haorang Zhong 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.2

Interval type-2 fuzzy logic controller (IT2FLC) owns good performance under uncertainty and nonlinearity environments while its optimization is hard and complicated. In this work, we propose an optimization method based on differential evolution with better and nearest option (NbDE) for interval type-2 fuzzy logic PID controller (IT2FL-PID-C) in order to control the position of hydraulic serial elastic actuator (SEA). Firstly, a simplified IT2FLPID-C structure with fewer parameters is proposed to reduce the difficulty of the optimization of IT2FL-PID-C. To balance its frequency and step performance, an objective function with weighted integral time absolute error and integral square error is given. Secondly, to investigate the performance of NbDE based IT2FL-PID-C, three experiments are conducted. A set of experiments is taken to determine the weight for fitness function. Then we compare NbDE with other algorithms. In addition, NbDE-IT2FL-PID-C is also compared with other optimization methods. At last, NbDE-IT2FL-PID-C is applied to hydraulic SEA and compared with PID. And a range for the weight of fitness function is given. The results have shown the superiority of NbDE with proposed fitness function to optimizeIT2FL-PID-C and the superiority of NbDE-IT2FL-PID-C to control the position of hydraulic SEA.

Fluorene- and arylamine-based photo-crosslinkable hole transporting polymer for solution-processed perovskite and organic light-emitting diodes

박정용,장지원,Xinyu Shen,장재호,곽선이,최효성,이보람,황도훈 한국고분자학회 2023 Macromolecular Research Vol.31 No.7

Solution-processable techniques, including spin-coating, roll-to-roll printing, and ink-jet printing, are suitable for large-scale manufacturing and flexible displays owing to their cost-effective and simple fabrication. These techniques are widely used in the fabrication of perovskite light-emitting diodes (PeLEDs) and organic light-emitting diodes (OLEDs). However, the energy level modulation between different functional layers, thickness control of the deposited layer, and interlayer mixing between the deposited and subsequent layers may affect the LED performance due to the poor solvent resistance of the deposited layer. Herein, we designed a novel poly[4,4'-(2-(4-((4-hexylphenyl)(phenyl)amino)phenyl)-9H-fluorene-9,9-diyl)bis(N,N-bis(4-butylphenyl)aniline)] (PFTPA-biTPA) as a hole transport layer (HTL) to modulate the hole injection. Subsequently, we introduced the bis(4-azido-2,3,5,6-tetrafluorobenzoate) (FPA) as a photo-crosslinker into the PFTPA-biTPA to prepare the photo-crosslinked HTL for both PeLED and OLED that inhibit attack from the processing solution and suppress the interlayer mixing between the deposited and subsequent layers. Both PeLEDs and OLEDs with photo-crosslinked PFTPA-biTPA exhibited maximum luminance of 175 and 4260 cd/m2 and maximum external quantum efficiency (EQE) of 4.16 and 10.86%, respectively, thus indicating superior properties to those without and with PFTPA-biTPA as the HTLs. Moreover, the photo-crosslinked PFTPA-biTPA showed significantly improved device stability compared to the reference devices having non-photo-crosslinked HTLs in PeLEDs.

Hydrogen Bond-Assisted Dual Passivation for Blue Perovskite Light-Emitting Diodes

( Song Jiaxin ),( Zhongkai Yu ),( Xinyu Shen ),박성흠,이보람 한국공업화학회 2023 한국공업화학회 연구논문 초록집 Vol.2023 No.1

Giant Zeeman splitting in nucleation-controlled doped CdSe:Mn²⁺ quantum nanoribbons

Yu, Jung Ho,Liu, Xinyu,Kweon, Kyoung Eun,Joo, Jin,Park, Jiwon,Ko, Kyung-Tae,Lee, Dong Won,Shen, Shaoping,Tivakornsasithorn, Kritsanu,Son, Jae Sung,Park, Jae-Hoon,Kim, Young-Woon,Hwang, Gyeong S.,Dobro Nature Publishing Group 2010 Nature Materials Vol.9 No.1

Doping of semiconductor nanocrystals by transition-metal ions has attracted tremendous attention owing to their nanoscale spintronic applications. Such doping is, however, difficult to achieve in low-dimensional strongly quantum confined nanostructures by conventional growth procedures. Here we demonstrate that the incorporation of manganese ions up to 10% into CdSe quantum nanoribbons can be readily achieved by a nucleation-controlled doping process. The cation-exchange reaction of (CdSe)<SUB>13</SUB> clusters with Mn<SUP>2+</SUP> ions governs the Mn<SUP>2+</SUP> incorporation during the nucleation stage. This highly efficient Mn<SUP>2+</SUP> doping of the CdSe quantum nanoribbons results in giant exciton Zeeman splitting with an effective g-factor of ∼600, the largest value seen so far in diluted magnetic semiconductor nanocrystals. Furthermore, the sign of the s–d exchange is inverted to negative owing to the exceptionally strong quantum confinement in our nanoribbons. The nucleation-controlled doping strategy demonstrated here thus opens the possibility of doping various strongly quantum confined nanocrystals for diverse applications.

CTLA4-Ig protects tacrolimus-induced oxidative stress via inhibiting the AKT/FOXO3 signaling pathway in rats

Long Jin,Nan Shen,Xinyu Wen,Weidong Wang,Sun Woo Lim,양철우 대한내과학회 2023 The Korean Journal of Internal Medicine Vol.38 No.3

Background/Aims: Although the conversion from tacrolimus (TAC) to cytotoxic T-lymphocyte-associated antigen 4-immunoglobulin (CTLA4-Ig) is effective in reducing TAC-induced nephrotoxicity, it remains unclear whether CTLA4-Ig has a direct effect on TAC-induced renal injury. In this study, we evaluated the effects of CTLA4-Ig on TAC-induced renal injury in terms of oxidative stress. Methods: In vitro study was performed to assess the effect of CTLA4-Ig on TAC-induced cell death, reactive oxygen species (ROS), apoptosis, and the protein kinase B (AKT)/forkhead transcription factor (FOXO) 3 pathway in human kidney 2 cells. In the in vivo study, the effect of CTLA4-Ig on TAC-induced renal injury was evaluated using renal function, histopathology, markers of oxidative stress (8-hydroxy-2’-deoxyguanosine) and metabolites (4-hydroxy-2-hexenal, catalase, glutathione S-transferase, and glutathione reductase), and activation of the AKT/FOXO3 pathway with insulin-like growth factor 1 (IGF-1). Results: CTLA4-Ig significantly decreased cell death, ROS, and apoptosis caused by TAC. TAC treatment increased apoptotic cell death and apoptosis-related proteins (increased Bcl-2-associated X protein and caspase-3 and decreased Bcl-2), but it was reversed by CTLA4-Ig treatment. The activation of p-AKT and p-FOXO3 by TAC decreased with CTLA4-Ig treatment. TAC-induced renal dysfunction and oxidative marker levels were significantly improved by CTLA4-Ig in vivo. Concomitant IGF- 1 treatment abolished the effects of CTLA4-Ig. Conclusions: CTLA4-Ig has a direct protective effect on TAC-induced renal injury via the inhibition of AKT/FOXO3 pathway.