대장직장암 환자의 술전 및 술후 혈청 CEA측정의 의의

손기섭,배진선,김인구,장일성,윤완희 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

This report is a clinical study of the significance of preoperative and postoperative serial check of plasma CEA levels in 36 cases of colorectal cancer who were admitted and treated at the Chungnam National University Hospital from Mar. 1987 to Oct. 1988 and the results were as follows: 1. The positive rate of preoperative CEA level(over 5ng/ml) in colorectal cancer was 58.3% In resection group, positive rate and mean CEA level were 53.6% and 7.6ng/ml respective and in unresection group they were 75% and 36.7ng/ml respectively (p<0.05). 2. The proportion of patients with abnormal CEA level(over 5ng/ml)was 33.3% in Dukes B, 46.2% in Dukes' C and 69.2% in Dukes'D. 3. The proportion of patients with abnormal CEA level(over 5ng/ml) was 25% in wel1.diffe rentiated type, 60.9%in moderately differentiated type and 37.5 % in poorly differentiated type. 4. The recurrence rate after curative resection in preoperative normal CEA(0~2.5ng/ml) group was presumed 0% in borderline(2.6~ 5ng/ml) group was 33.3%, in high(over 5ng/ml) group was 16.7% 5. In 3 patients with postoperative liver metastasis had the highest frequency of elevated level(over 60ng/ml), whereas those with local recurrence had a lower frequency of elevated level. 6, A patient had second look procedure include segmental resection of local recurrence, buthe was dead of peritoneal caricinomatosis several months later.

급성 골수성 백혈병 환자의 말초혈액 단핵구로부터 수지상세포로의 분화 유도

손상희,이대희,박재선,어완규 고신대학교 의학부 2002 高神大學校 醫學部 論文集 Vol.17 No.1

Background Dendritic Cells (DCs) are the most potent naturally occuring antigen presenting cells and play an important role in T-cell activation. DCs may be suited for in vivo immunotherapy for its capability to stimulate naive T cell. Effective presentation of tumor antigens is fundamental to strategies aimed at enrolling the immune system in eradiation of residual disease after conventional treatments. Myeloid malignancies provide a unique opportunity to derive dendritic cells (DCs), functioning antigen presenting cells, from the malignant cells themselves. These may then co-express leukemic antigens together with appropriate secondary signals and be used to generate a specific, antileukemic immune response. Methods In this study, peripheral blood mononuclear cells (PBMCs) from 5 patients with acute myeloid leukemia (AML) were cultured with combinations of Flt-3 Ligand (FL), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4(IL-4), and tumor necrosis factor-α(TNF-α), and development to DCs was assessed. Peripheral blood mononuclear cells (PBMCs) were obtained from 40-60㎖ of peripheral blood of patients with acute myeloid leukemia (AML) by Ficolling. Cells were resuspended in X VIVO-20 medium supplemented with FL (100ng/㎖), GM-CSF(100ng/㎖), IL-4(50ng/㎖), and TNF-α(20ng-㎖) and seeded into T75 culture flasks at 3×107/50㎖. Results After 12 days in culture, cells from 5 samples exhibited morphological and immunophenotypic features of DCs, including expression of CDla, CD83, and CD86. Conclusion This study indicates that cells with enhanced antigen-presenting ability can be generated from PBMCs of patients with acute myeloid leukemia, that these cells can effectively prime autologous cytotoxic T cells in vitro, and that they may be used as potential vaccines in the immunotherapy of AML.

교통사고환자 1046예에 대한 분석 및 Modified CRAMS Scale

윤완희,장일성,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

We retrospectively evaluated the nature, distribution, and pattern of the traffic accidents in 1046 cases of traffic accidents transported to chungnam National University Hospital from Jan. 1987 Dec. 1988. We also evaluated the ability of the CRAMS Scale to predict injury severity and mortality, and the results were as follows: 1. The age distribution was most prevalent between 25-44 years(44.6%), the ratio of male to female was 2.9:1, and the ratio of male of female of the children and old age group was 1.6:1. 2. There was no significant difference in seasonal distribution. 3. Of 1046 cases, 39.8% was associated with Orthopedic Surgery, Neurosurgery, Plastic Surgery, and Genera Surgery were 38.8%, 20.3%, and 10.0% respectively. 4. In injury mechanism, motor vehicle accident was highest in incidence(50.7%), pedestrian accident, motorcycle accident, and bicycle accident were 23.4%, 18.6%, and 7.3% respectively. 5. The most frequently injured body region was head and neck(65.6%), and then extremity and pelvis was 37.3%, and abdomen was 10.9%. 6. Traffic accident developed in urban area was 1.4 times more frequent than rural area. 7. In elapsed time from arrival to death, 77.6% was died within first 24 hours and 33.6% was died at scene or less than 1 hour. 8. The overall mortality rate was 11.9% and in mortality rate according to department, Neurosurgery was 52.8%, Chest Surgery was 20.8%, and General Surgery was 13.6%. 9. Using a cuttoff score 7 or less, the Modified CRAMS scale identified the 16.6% of the traffic accident population which were critically ill as demonstrated by a 67.2% mortality rte compared to a 0.9% mortality of those with score of 8 or greater.

심장박동 측정 센서회로 IC 설계에 관한 연구

이우람,이완직,원창수,손상희 청주대학교 산업과학연구소 2008 産業科學硏究 Vol.26 No.1

Interface IC design for heart beat measurement sensor is proposed and executed by using current control schmitt trigger. The function of interface IC is to measure the heart beat using PPG from sensor. This circuit is composed of analog circuit that converts current signal of sensor to voltage and digital circuit that converts it to digital value. Applying OTA to schmitt trigger and oscillator, operation speed is faster and linear error is smaller than the conventional one. Besides, follow features such as low power consumption, simple configuration and high resolution, are realized by IC design.

肺癌에 있어서 職業과의 關聯性 硏究

김두희,박완섭,손지연,이종영,홍성철 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2

폐암에 대한 직업의 관련성을 밝히기 위하여 1993년 11월에서 1994년 8월까지 대구 시내 2개 종합병원에 입원하여 병리 조직학적으로 진단된 168명의 폐암 환자와 같은 병동에 있는 내과 환자를 연령군과 성별로 빈도 짝짓기하여 168명의 대조군으로 선정하였다. 직업력과 유해 물질의 폭로력, 거주 환경, 흡연에 관한 정보는 설문지를 통하여 얻었다. 흡연 상태를 보정하고 연령군과 성별에 대한 짝지은 자료에 대하여 직업과 산업 분류에 대한 폐암 발생의 상대 위험은 농업 종사자에서 유의하게 높았고(OR=1.84 ; 95% 신뢰구간 1.16-2.94) 생산직, 가내 수공업, 운수창고통신업, 숙박음식업, 건설업에서도 높게 나타났으나 유의하지 않았다. 그러나 가사(OR=0.31 ; 95% 신뢰구간 0.11-0.89), 관리직(OR=0.22 ; 95% 신뢰구간 0.08-0.61), 공공 행정업(OR=0.23, 95% 신뢰구간 0.08-0.66), 공공 사회및 개인 서비스직(OR=13 ; 95% 신뢰구간 0.03-0.56)에서는 폐암 발생의 상대 위험이 유의하게 낮았으며, 전문직, 도소매업에서도 낮게 나타났으나 유의하지 않았다. 거주 환경에 따른 폐암 발생의 상대 위험은 연탄난로 사용자(OR=3.00 ; 95% 신뢰구간 1.06-8.52)와 농약 사용시(OR=2.14 ; 95% 신뢰구간 1.36-3.37)에 통계학적으로 유의하게 높았고, 공장지대 거주자에서도 높게 나타났다(OR=1.97 ; 95% 신뢰구간 0.92-4.27) 그러나, 중앙집중식난방사용자(OR=0.5 ; 95% 신뢰구간 0.28-0.89)에서는 낮게 나타났으며 통계학적으로 유의하였다. 유해 물질 폭로에 따른 폐암 발생의 상대 위험은 농약(OR=2.07 ; 95% 신뢰구간 1.31-3.26)과 용접 및 흄(OR=10.56 ; 95% 신뢰구간 1.07-103.90)에 폭로시 유의하게 높게 나타났다. 흡연에 의한 폐암 발생의 상대 위험은 비흡연자에 비하여 흡연자(OR=3.98 ; 95% 신뢰구간 1.88-8.45)와, 과거흡연자(OR=4.50 ; 95% 신뢰구간 1.99-10.16)에서 높게 나타났다. 이상의 결과로 비록 흡연이 폐암의 중요한 원인이지만 흡연의 영향을 보정한 후에도 대기오염 및 직업과 폐암과 관련성이 있음을 보여주고 있으며, 앞으로 이러한 직업에서 어떠한 위험 요인이 있는지에 대한 추구가 필요하여 그에 대한 예방 대책을 밝히는 연구가 필요하다. The relationship between occupation and risk of lung cancer was analyzed in hospitalbased case-control study conducted in Taegu on 168 patients with histologically confirmed lung cancer and age group(30-39, 40-49,etc) and sex frequency-matched 168 controls admitted into hospitals for other internal medicine problems. A self adminstered questionnaire was used to obtain occupation, resident environment, smoking histories and specific agent exposure history. The conditional logistic regression was used to estimate oddsratio for age group and sex frequency matched, after controlling for smoking status(non smoker, exsmoker, current smoker) Significant association was observed for farmer[odds ratio(OR), 1.84 ; 95% confidence interval(CI), 1.16-2.94], briquette stove users[OR, 3.0 ; 95% CI, 1.06-8.25], agricultural chemicals users[OR, 2.14 ; 95% CI, 1.35-3.37], and welding/fume exposures[OR, 10.56 ; 95% CI, 1.07-103.90]. Although there were elevated risks associated with production, home industry, transportation/storage/communication, lodgement/food worker and construction, none were statistically significant. The effect of smoking was showed significantly increased risk for exsmoker [OR, 4.50 ; 95% CI, 1.99-10.16] and current smoker [OR, 3.98 ; 95% CI, 1.88-8.45]. As results, although smoking is important risk of lung cancer, occupation and resident environment are showing association of lung cancer. So further evaluation is necessary for occupation risk and take preventive measures.

시멘트 킬른 더스트를 사용한 콘크리트의 역학적 특성에 관한 연구

전충근,차천수,차완호,손성운,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

This paper is intended to investigate the properties of fresh and hardened concrete, in which cement and fine aggregate are replaced with cement kiln dust(CKD), for effective using method of CKD, a by-product produced in the process of making cement. According to the results, as the replacing ratio of CKD increases, slump and air content of concrete decreases remarkably due to an increase of viscosity and filling of the pores respectively. As the properties of setting, setting time are shortened with an increase of the replacing ratio of CKD. Compressive strength increases due to the filling effect of the pores in comparison with plain concrete, and compressive strength ratio is high at early age.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

중증 세균 감염 및 내성균에 대한 Fosfomycin의 치료효과

김찬기,노승무,윤완희,장일성,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

A Clinical study for the therapeutic effect of Fosfomycin was made in 20 cases with the severe infection and the resistent organisms against the other antibiotics. Fosfomycin was administered intravenously 2-4 gm daily in two divided for an average of 13.1 days. The overall effectiveness was 90% without any significant side effect and the effectiveness for the resistant organisms(13 cases) against the other antibiotics was 84.6%. These results demonstrated that Fosfomycin can be used effectively in the treatment of the severe infection and the resistant organisms against the other antibiotics.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1