국제기구 및 외국에서 농산물중 농약잔류 허용기준의 설정절차

이미경,홍무기,박건상,최동미,임무혁,이서래 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

This study was undertaken to find out necessary measures to improve the tolerance setting system of pesticide residues on food crops in Korea which is scientifically reasonable and harmonizable with international standards. Information on tolerance setting systems of pesticide residues by Codex Alimentarius Commission, Joint FAO/WHO Meeting of Experts on Pesticide Residues, USA, EU, Japan and Taiwan was collected and analyzed. On the basis of information in the above countries, necessary actions to be taken by the Korean regulatory authorities were recommended with respect to priority setting, maximum residue limits (MRLs) setting based on field residue data, group MRLs, minor crop problems, quantitation limit and dietary intake assessment.

식품의 오염과 안전성 평가

李瑞來 中央大學校 食糧資源硏究所 1990 食糧資源硏究所 論文集 Vol.2 No.1

한국인을 위한 식품 평균소비량(1990년대) 자료의 최적화

이서래,이효민,허근,이미경 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

국내에서는 식품수급표와 국민영양조사가 지난 수십년에 걸쳐 두개의 중앙부처에서 각각 수행되어 왔다. 그러나 근년에 들어와 두가지 통계자료간의 격차가 수용할 수 없는 수준에 도달하고 있다. 그 이유로서는 지난 30년간 공업화 및 도시화에 따른 식습관의 급격한 변화에도 불구하고 조사방법의 개선이 뒤따르지 못했기 때문으로 추정된다. 따라서 현재 이용가능한 국민영양조사에 의한 식품소비량 데이타를 식품수급표를 감안하면서 최적화하였다. 최근 1991-95년 기간중 개별 식품항목의 조정 소비량이 곡류, 감자류, 당류제품, 두류, 견과종실류, 과일류, 채소류, 육류, 난류, 우유제품, 유지류 및 수산물에 대하여 제안되었다. 소맥제품, 두류제품, 김치류 및 우유제품을 포함하는 가공 식품에서는 그들이 원료성분으로 계산하기 위한 잠정적인 환산계수를 제안하였다. 식품들의 공급량 데이타와 섭취량 데이타 간의 큰 편차는 국민영양조사에서 계절적 차이와 아울러 가공식품 및 외식에 의한 섭취량이 무시되었기 때문으로 지적되었다. 여기에서 제안하는 조정된 식품소비량 데이타는 앞으로 새로운 절차에 의한 국민건강영양조사 결과가 나와 검증될 때까지 평균소비량을 위한 국가대표치로 활용되기를 기대한다. 1) In Korea, food balance sheet and national nutrition survey have been conducted in parallel by Central Government for several decades. However, the gap between the two statistics became larger to reach an intolerable level in recent years, likely due to the limitation of dietary survey method in spite of the drastic change in dietary habits after industrialization and urbanization during last three decades. The currently available data from national nutrition survey was optimized by taking in to account of the food balance sheet. Adjusted consumption data for individual food items during the period of 1991-95 were given in tabular forms for groups of cereals, potatoes, sugars & sweets, legumes, nuts & seeds, fruits, vegetables, meats, eggs, dairy products, oils & fats and fishery foods. Conversion factors of processed foods including wheat products, soybean products, Kimchi and dairy products into their raw materials were tentatively proposed. Great differences between supply data and intake data of food commodities were pointed out to be likely due to the seasonal variation and ignorance of processed foods and dining-out in the national nutrition survey. It is recommended to utilize these optimized food intake data as the nationally-representative one until new data from the national health and nutrition survey undertaken after modified procedures since 1998 are obtained and verified in future.

식물성 식품중 페놀성 물질의 몇가지 생리활성

이정희,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

페놀성 물질의 함량이 높게 분석된 9개 시료(감잎, 들깨씨, 모과, 생강, 호두 쑥, 칡뿌리, 메밀, 수수)를 선택하여 소화효소에 대한 활성저해, 식품오염세균에 대한 항균성, 그리고 Ames 법에 의한 돌연변이원성 및 항돌연변이원성을 살펴보았다. 소화효소 활성저해를 실험한 7개시료중 모과만이 a-amylase에 대해 97%의 활성저해를 보였고, 무경쟁저해를 나타냈다. protease에 대하여 모과, 감잎, 쑥은 각각 86%, 51%, 20%의 저해효과를 보였고, 이때 쑥시료는 비경쟁저해를 나타냈다. Lipase는 모든 식품에 의해 50%이상 활성화되었다. a-amylase의 활성저해는 축합형 탄닌함량과 높은 상관관계 (r=0.89)를, protease는 총페놀 함량과 높은 상관관계(r=0.84)를 보였다. 표준물질인 tannic acid, gallic acid, caffeic acid, catechin과 7개 식품시료의 총페놀 분획을 얻어 항균성 효과를 실험한 결과 tannic acid는 3균주에 대해 비교적 높은 항균력을 보였고 둘깨, 수수, 칡뿌리는 E coli에 대해 높은 항균력을 보인 반면 salmoncella enteritidis와 streptococcus faecalis에 대해서는 시료들간에 큰 차이를 보이지 않았다. 표준물질 및 9개 식품의 총페놀 분획은 모두 Salmonella typhimurium TA98과 TA100에 대해 돌연변이원성을 보이지 않았다. 한편 benzo[a]pyrene을 이용한 항돌연변이원성 실험결과 tannic acid는 TA98의 경우 40~80%, TA100의 경우 70~95%의 강한 항돌연변이원성을 나타냈다. TA100에 대해 모과는 30%의, 호두는 44%의 항돌연변이원성을 보였으나 기타 식품에서는 항돌연변이원성을 관찰할 수 없었다. Nine plant foods(persimmon leaf, perilla seed, Chinese quince, ginger root, walnut, mugwork leaf, arrowroot, buckwheat and sorghum) rich in phenolic substances were examined for their effects on the digestive enzymes, food-poisoning bacteria and mutagenicity/antimutagenicity by Ames test. Among tested samples, Chinese quince significantly inhibited the a-amylase activity (97%), exhibiting an uncompetitive inhibition type. Protease activity was inhibited by Chinese quince (86%), persimmon leaf (51%) and mugwort leaf (20%), in which mugwort extract exhibited a noncompetive type. Lipase was activated >50% by all samples. The inhibition of a-amylase was highly correlated with the content of condensed tannin (r=0.89) and the inhibition of protease, with total phenolic content (r=0.84). Total phenolics fraction of tested samples showed the growth inhibition toward E. coli, Streptococcus faecalis and Salmonella enteritidis, in which the effect of perilla, sorghum and arrowroot was the highest for E. coli. Standard phenolics and food samples did not show any mutagenicity toward Salmonella typhimurium TA98 and TA100. Tannic acid inhibited the mutation of the two strains by benzo[a]pyrene whereas total phenolics fractions of Chinese quince and walnut exhibited antimutagenicity to a lesser extent.

톨루엔 디이소시아네이트 폭로 근로자들의 작업방법에 따른 대사물질의 비교 및 면역능에 관한 연구

이수일,조병만,황인경,이철호,박정래 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.3

Following recent advanced industrialization, the amount of polyurethane to use as thermal insulating materials, upholstery, mattresses and packing materials in automotive and furniture industry is increasing world-widely, and the number of polyurethane-producing worker will be increased. Because the numerous organic solvents are used in polyurethane-producing factory, the workers in this work site is exposed to many organic solvents. Of the organic solvents, Toluene Diisocyanate(TDI) has many hazardous effects to human. The effects of TDI on human are the irritation to respiratory mucosa and gastrointestinal symptoms. Conjunctival irritation, dermal inflammation (redness, pain, vesicular formation) and gastrointestinal symptom(nausea, vomiting. abdominal pain) are reported just after short-term exposure of TDI. TDI is known to give rise to bronchial asthma, as the immune disorder. And because of strongly volatile characteristics of TDI, it is suggested as a more injurious material to human health, especially human immune system, than other organic solvents. Bronchial asthma inducing mechanism of TDI is not clearly known, but on the analogy of TDI induced symptoms and recent studies, early-onset asthma is type I hypersensitivity reaction mediated by immunoglobulin E(IgE), and late-onset asthma is maybe type III hypersensitivity reaction by circulating IgG. And we know that the complicated human immune function is likely to move in such that mechanisms, there are not studies on immune indices evaluating the bronchial asthma-related immune function. The evaluation of change patterns of humoral immunity including IgE and IgG and cellular immunity including T-helper cell. T-suppressor cell and T-cytotoxic cell will be helpful to evaluate exposure degrees and prognosis in TDI exposed workers. Because TDA(toluene diamine) as a biological exposure index of TDI becomes the focus of interest, we know that a study on the correlation between urinary TDA and air TDI and immunological indices will make a contribution to biological effect monitoring indicies. We examined human immunity indicators such as WBC, %Lymph (percentile of Lymphocyte in WBC), %T-cell(percentile of T-lymphocyte in total lymphocyte). CD4, CD8, C3, C4, IgA, IgG, IgM, IgE in peripheral blood to evaluate the health hazard of the TDI-exposed workers. And we examined TDA to evaluate correlation between exposure and effect. Total 90 subjects was selected, 45 workers who worked in the polyurethane-producing factories as an exposed group, and 45 cases who were office workers(10 cases), other blue collors(27 cases), and medical college students(8 cases) as a control group. And the results were as follows; 1. The logarithm of IgE-Log10(IgE)±SD-in peripheral blood of a exposed group was significantly higher than a control group, 2.22 ±0.62 in case group compared with 1.98±0.53 in control group. (p<0.05) 2. IgA and IgM in the polyurethane-producing workers were 261.02±83.12㎎/㎗, 151.97 ±59.64 ㎎/㎗, respectively, and 292.77±100.45, 179.17±100.78 in control group. IgA and IgM was slightly lower in polyurethane-producing group than control.(p>0.05) 3. WBC, %Lymph. %T-cell, C3, C4, CD4, CD8, CD4/CD8 ratio and IgG in case group were 6.391.1 ea/㎖, 37.53 %, 59.54 %, 76.68 ㎎/㎗, 0.76×10(9) ea/L, 0.63×10(9) ea/L, 1.39, and 1606.29 ㎎/㎗, respectively, and 6,974.7 ea/㎖, 35.12 %, 59.64 %, 71.95 ㎎/㎗, 33.94 ㎎/㎗, 0.80×10(9) ea/L, 0.61×10(9) ea/L, 1.39, and 1581.51 ㎎/㎗ in control group. There was no statistical significance between two groups.(p>0.05) 4. In the comparison of each other companies, average of individual urinary TDA in polyurethane paint manufacturing companies is higher than that of polyurethane sponge foaming companies. And, the concentration of 2.6-TDA which is a metabolite of well-vaporized 2.6-TDI is higher than that of 2.4-TDA in the polyurethane sponge foaming companies. But, the concentration of 2.4-TDA which is a metabolite of illvaporized but well skin-absorbed 2.4-TDI is higher in polyurethane paint manufactures. 5. There were no statistical significance in the correlations between individual urinary TDA and immunologic indices.

한국식품 중 잔류농약의 종양유발성 평가

이미경,이서래 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

국내에서 소비되고 있는 농약 중 종양유발지수가 알려진 12개 성분의 식품군별 식이섭취량과 그들의 위해성을 평가하였다. 한국인에 대한 종양유발성(70년 평생을 통한 부가적 종양발생율)은 농약잔류 허용기준에 의한 경우 2.17x10^-3, 실제 잔류량 최대치에 의한 경우 4.33x10^-5, 실제 잔류량 평균치에 의한 경우 5.10x10^-6으로서 미국EPA의 무시될 수 있는 위험기준인 1x10^-6을 초과하였다. 발암성 농약의 남용에 대한 국민들의 불안을 해소하기 위해서는 종양유발성 농약에 대한 체계적인 후속연구가 추진되어야 할 것이다. Dietary intake and oncogenic risk of 12 pesticides used in Korea whose oncogenic risk od 12 pesticides used in Korea whose oncogenic potency was known were assessed from published data. Dietary oncogenic risk (excess tumor incidence for a 70year human life span) for Korean population was estimated to be 2.17×10^-3 on the basis of legal maximum residue liomit, 4.33×10^-5 on the basis of maximum practical residue level and 5.10×10^-6 on the basis of mean practical residue level of examined pesticides, all of which exceeded the negligible risk standard 1×10^-6 of US EPA. A systematic follow-up study on those oncogenic pesticides should be undertaken in order to mitigate the people's worry about the cancer risk by the abuse of pesticides in food production.

캔 및 병 오렌지쥬스의 저장온도에 따른 Q_10값 및 품질수명의 산정

이남경,윤재영,이서래 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

현재 국내에서 유통되고 있는 캔 및 병 오렌지쥬스를 20,30,40,50℃에서 24주 동안 저장하면서 4주 간격으로 개봉하여 중금속(Sn,Fe,Pb) 함량, 비타민 C 함량, 갈색도와 pH의 변화에 대한 측정치로부터 온도별 활성화 에너지와 Q_10값을 계산한 다음 shel-life를 예측하였다. 캔쥬스의 품질지표로 삼을 수 잇는 것은 Sn,Fe,Pb함량, 비타민 C 함량이었고, 병쥬스에서는 비타민 C 함량, 갈색도이었으며 이들 품질지표에 대한 Q_10값은 1.3~2.0이었다. 쥬스를 50℃에 저장시 품질 허용한계에 도달하는데 요하는 기간과 Q_10 대표값으로 부터 shel-life를 계산하면 캔쥬스의 경우 10,20,30℃에서 각각 10,6,3개월 이었고, 병쥬스의 경우 각각 24,12,6개월이었다. 국내에서의 쥬스 유통온도를 평균 15℃로 가정할 때 적정 품질수명은 캔쥬스 8개월, 병쥬스 18개월로 평가되엇다. 캔 오렌지쥬스의 안전성을 확보하기 위해서는 식품공전Pb 허용기준(0.3㎎/㎏)의 재검토와 아울러 캔 재질의 개선을 위한 연구노력이 요구된다. Canned and bottled orange juices currently sold in Korean market were stored for 24 wweeks at 20, 30, 50 and 50℃ and analyzed after opening at 4 week intervals. Activation energy, Q_10 values and shelf-life at respective temperatures were calculated from the data on various quality indexes. Proposed indexes were lead, tin, iron and vitamin C contents in canned juices and vitamin C content and browning index in bottled juices and Q_10 values for these parameters were in the range of 1.3~2.0. Shelf-life calculated for canned juices at 10, 20 and 30℃ were 10, 6 and 3 months and that in bottled juices, 24, 12 and 6 months, respectively. When the distriburion temperature of juices in Korea is assumed to be 15℃ average, the optimum shelf-life was estimated to be 8 months in canned juices and 18 months in bottled juices. In order to ascertain the safety of canned orange juices, efforts are needed to re-examine the recommended distribution period, legal limit for lead and improvement of container materials.

캔 오렌지쥬스의 중금속 함량 및 개봉 저장 중의 변화

이혜선,이서래 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

현재 국내에서 유통되고 있는 캔 오렌지 쥬스의 중금속 (Pb, Sn) 함량과 쥬스의 개봉 저장중 이들 중금속의 함량변화를 습식 분해 후 추출과정을 거쳐 원자흡관광도계로 분석하였다. 오렌지 쥬스 53개 시료중 중금속의 평균 농도는 Pb 0.225 mg/kg, Sn 40.7 mg/kg 이었다. 쥬스의 유통기간 경과에 따라 Pb 농도는 거의 증가하지 않았으나 Sn 농도는 한달이 경과할 때마다 평균 0.66 mg/kg씩 증가하는 것으로 나타났다. 개봉한 시료를 그대로 실온과 냉장고에 보관한 결과 Pb 농도는 7일 경과후에 저장 전의 1.7~1.8배로 증가하였고 Sn 농도는 1일 평균 20%씩 증가하여 7일 경과 후에 저장 전의 약 3배로 증가하였다. 또한 개봉한 시료를 유리 용기에 옮겨 담아 실온과 냉장고에 보관한 결과Pb와 Sn 농도는 저장 기간중 큰 변화가 없었다. 결론적으로 보아 유통되고 있는 캔 오렌지 쥬스는 권장 유통기한 이내의 제품에서도 Pb 함량이 법적 기준을 초과하는 빈도가 18%나 되므로 생산 공정 또는 유통기간 중의 규제용 검색을 강화해야 될 것이며 캔 오렌지 쥬스를 개봉한 후에는 Pb나 Sn의 농도가 크게 증가하므로 사용상의 주의를 필요로 한다. The average concentration of Pb in 53 samples of canned orange juice currently sold in Korean market was 0.225 mg/kg, and that of Sn, 40.7 mg/kg. There was no appreciable difference in Pb concentration according to elapsed time after manufacturing, whereas Sn concentration increased 0.66 mg/kg per month. During the storage at room temperature or in refrigerator after opening, the Pb concentration increased slowly, reaching 1.7 to 1.8 times of original concentration, whereas Sn concentration increased by 20% per day, resulting in 3 times of original concentration after 7days. There were no serious changes in Pb and Sn concentration in storage at room temperature or refrigerator for 3 days, when juice samples were opened and transferred to glass container. It is needed that detailed inspection by undertaken to monitor the contents of heavy metals in canned orange juice, since 18% of samples within recommended distribution period exceeded the legal standard for Pb, and recommended that more attention be paid in handling canned orange juice after opening, in order to avoid the hazard from heavy metals.

한국인의 농축산식품 섭취량의 표준화(1986~90)

이미경,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

한국인을 위한 식품중 유해성분의 기준설정 및 안정성 평가에 필요한 식품계수를 제안하기 위하여 1986~90년에 걸쳐 국민영양조사에 의한 식품섭취량과 식품수급표에 의한 공급량 자료로부터 농축산식품 원료에 대한 식품 종류별 섭취량을 최적화 하였다. 농축산물의 1인당 1일 평균 섭취량은 1.0 kg으로 조정하였으며 곡류 422g, 감자류 29g, 두류 17g, 견과종실류 5g, 과일류 97g, 채소류 300g, 육류 49g, 난류 20g, 우유류 72g 이었다. In order to provide food factors necessary for tolerance setting and risk assessment of hazardous substances in foods, dietary intake of farm produces by Korean population was optimized from national nutrition survey and food balance sheet during the period of 1986~90. Total daily intake of agricultural and livestock produces was normalized to 1.0 kg on the average, which consisted of 422 g cereals, 29 g potatoes, 17 g legumes, 5 g nuts & seeds, 97 g fruits, 300 g vegetables, 49 g meats, 20 g eggs and 72 g milks.

학교체육 활성화 방안에 대한 연구(Ⅰ) : 강원대학교 교양체육 운영방법의 개선을 중심으로

이광재,문병용,노성규,박기동,홍관이,한상준,유옥재,엄기진,정청자,오수일,김윤래,박장평,부기원 江原大學校附設 體育科學硏究所 1986 江原大學校附設體育科學硏究所論文集 Vol.- No.11

Try to find a scheme for activation to College Physical Education, this Report informs the various kinds of Survey and process those were projected for improvement of management methods on Cultural Physical Education in Kang Weon National University. From experimentally carried out "Sports events Choosing System" not as usual be inforced "Total practical Skill System", Following Positive effects and sujestions were Presented; 1. "Sports events choosing system" gave birth to more students' interesting and more voluntary participation to the sports events they chose, and sports skill and instruction level was elevated due to charge of expert according to each sports events. This system would be managed continuously hereafter for it related to life sports. 2. This System needs to closed cooperations between College and Community, for the facilities of community are utilized by college. 3. To complete the plan for activation of college Physical Education, additionally, the successional study ; namely "autonomous extracurricular sports activity" should be excuted hereafter.