쑥, 진피, 두충이 고지혈증 흰쥐의 체내 지질대사에 미치는 영향

김지현,왕수경 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

본 연구는 생후 4주된 Sprague-Dawley rats을 1% cholesterol, 30% lard를 첨가조제한 고지방 식이로 3주간 사육하여 고지혈증을 유발시킨 후 쑥, 두충, 진피를 섬유질원으로 하여 식이중 총 식이섬유 함량이 10%가 되도록 조제한 식이로 흰쥐를 6주간 사육했을 때의 체내 지질대사에 미치는 영향을 cellulose군과 비교 관찰하였다. 그 결과 실험 동물의 총 식이섭취량에 있어서 cellulose군, 진피군이 쑥군, 두충군보다 유의적으로 높았고, cellulose군과 진피군 비교에서는 대조군인 cellulose군이 높았다. 체중 증가량은 cellulose군이 나머지 세 군에 비해 유의적으로 높았다. 지방 흡수율은 두충군이 LDL-콜레스테롤은 쑥, 두충, 진피군이 cellulose군에 비해 비해 유의적으로 낮았고, 특히 두충군이 가장 낮았다. 중성지방은 cellulose군에 비해 세군이 유의적의로 낮았고, HDL-C/TC에 있어서는 cellulose군에 비해 나머지 세 군이 유의적으로 높았다. 두충, 쑥, 진피군 중에서는 두충군이 가장 높은 경향을 보였다. 혈당에 있어서는 통계적 유의차는 없었지만 쑥, 진피군이 다소 낮은 경향을 보였고, cellulose군이 가장 높았다. 간의 총지질과 총콜레스테롤 함량은 cellulose군이 다른 세 군에 비해 유의적의로 높았고, 중성 지방에 있어서는 cellulose군이 유의적으로 낮았다. 변의 총지질과 총콜레스테롤 농도는 두충, 쑥군이 celluloserns, 진피군에 비해 유의적의로 높았고, 진피군도 cellulose군에 비해 다소 높은 경향을 보였다. 중성지방은 진피군에 비해서 대른 세 군이 유의적으로 높았고 수분을 포함한 변중량, 건중량에 있어서는 cellulose군에 비해 쑥, 진피군이 유의적의로 높았으며 두충군도 cellulose군에 비해 다소 높은 경향을 보였다. 수분함유량은 진피군이 유의적으로 높았고, 두충군, 쑥군이 cellulose군에 비해 높았다. 이상의 연구 결과 진피는 혈중 총지질, 총콜레스테롤 수준 저하, 그리고 배변에 효과적이고 두충은 혈중의 HDL-콜레스테롤 증가, 총콜레스테롤 감소에 효과적의로 나타나 진피, 두충이 우수한 지질 대사 개선 효과를 나타내는 것으로 보인다. 이러한 결과로 보아 cellulose와 같은 불용성 식이섬유보다 수용성과 불용성 식이섬유를 동시에 함유하고 있는 식품 자체가 고지혈증·동맥경화의 예방이나 치료에 효과가 있을 것으로 기대된다. This study was performed to investigate the influence of dietary foods from three Chinese medicinal herbs(Mugwort, Dried orange peel and Duchung) on lipid metabolism in rats. Male rats of Sprague-Dawley strain were divided into four dietary groups(Cellulose(CL), Mugwort(MW), Dried orange peel(OP), Duchung(DC)) after three weeks of hyperlipideniic dieting and were fed ad libiturn each of the experimental diets for six weeks. The fiber sources of the experimental diets were prepared by drying and milling mugwort, orange peel, duchung and cellulose. Each of the fiber sources was mixed into the diet to achieve 10 8% levels of total dietary fiber. Levels of apparent fat absorption exhibited a significant decrease in the MW and DC groups. The OP group also showed a decreasing trend, but to a lesser extent. The levels of serum total lipid were not significantly different among the four experimental groups. Total serum cholesterol levels were significantly lower in the OP group than in both the CL and MW groups. Serum TC; and LDL-cholesterol levels were significantly higher in the CL group than other groups. Serum HDL-cholesterol levels were significantly lower in the CL group than in the other groups. Total lipid and total cholesterol in liver were significantly higher in the Cl, group than in other groups and TG in liver was significantly lo\w in the CL group than in the other groups. Total lipid and total cholesterol in feces displayed a significant increase in the MW and DC groups when compared with the CI, group. The OP group also showed an increase compared to the CL group. (Korean J Nutrition 30(8) : 895~903, 1997)

고 아밀라아제 혈증을 보인 급성 Lithium Carbonate 중독증 1례

홍현상,박흥수,정염성,노왕구 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.2

Lithium carbonate is a widely used and invaluable drug in the treatment and prevention of manic-depressive illness. However, lithium cardonate may induce multisystemic adverse effects and toxic effects that, when not recognized, may lead to severe, life-threatening complications. The authors experienced a manic patient who showed severe lithium toxic reactions with hyperamylasemia. The patient was 41-year-old woman who had received lithium carbonate treatment for 3 months. The patient's symptoms and signs of toxic reaction had disappeared after hydration, insulin therapy, and hemodialysis.

난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

난소화성 덱스트린이 흰쥐의 단백질과 지방이용률에 미치는 영향

김지현,왕수경 대전대학교 기초과학연구소 1995 自然科學 Vol.6 No.-

식이 중 난소화성 덱스트린이 휜 흰 쥐의 단백질과 지방의 이용율에 미치는 영향을 조사하기 위해 Sprague-Dawely계 (평균체중 109g) 흰 쥐를 3군으로 나누어 control군으로 무섬유소군(NC), 섬유소간의 비교를 위해 5% pectin군(NP), 5% 난소화성 덱스트린군(ND)으로6주간 사육하였다. 얻어진 결과를 요약하면 다음과 같다. 실험기간중 식이 섭취량은 ND군이 다른 군보다 높은 경향을 보였으나 체중증가량과 식이효율은 ND, NP군이 NC군보다 낮았다 (p<0.05). 간, 비장, 신장의 무게는 각 군간에 유의적 차는 없었다. 그러나 부고한 지방은 NP군과 ND군이 낮은 경향을 보였다. 단백질 섭취량은 NP군이 높았고, 뇨중 단백질 배설량은 ND군이 높았으며, 변중 단백질 배설량은, NP, ND군이 NC군보다 낮게 나타나 결과적으로 단백질 흡수율, 보유율은 실험군간 유의적 차를 보이지 않았다. 변 중 지방 배설량은 NP군이 높았고 ND군도 NC군보다 높은 경향을 보였다. 지방의 흡수율은 NP군이NC군보다 유의적으로 (P<0.05) 적었고, ND군도 NC군보다 적은 경향을 보였다. 따라서 난소화성 덱스트린은 식이효율이 낮아 체중 감소 효과를 나타냈으며, 이는 단백질 이용율의 감소보다는 지방이용율의 감소의 결과로 나타난 것으로 보인다. 앞으로 당 대사와의 관계와 병행해서 연구되어야 할 것으로 사료된다. This study investigated the effect of indigestible dextrin on bioavailabilities of protein and lipid. Sprague-Dawley male rats weighing 108g were blocked into three groups; control diet (NC), control + 5% pectin (NP), control + 5% indigestible dextrin (ND). Experimental rats were fed ad libitum each of experimental diets for six weeks. The results are summarized as follows. 1) food intake was generally higher in ND group than in other groups and weight gain and food efficiency ratio were significantly lower in ND, NP groups than in NC group(p<0.05). 2) Weight of liver, kidney and spleen were lower in ND group than in other groups, epididymal fat pads was lower in ND and NP groups than in NC group. 3) Protein bioavailabilities was not significantly different in three dietary groups. 4) Fat bioavailabilities was significantly lower in NP group than in NC group(p<.05) and tended to be lower in ND group than in NC group. Accordingly, weight reduction shown in ND group seems to be resulted from the decreased fat bioavailabilities.

셔틀 프로토콜의 이론적 고찰

강현중,왕종수 瑞逸大學 1998 論文集 Vol.16 No.-

We prove the efficient technique(SHUTTLE polling method) for management traffic generated by the polling method. Because generating rate of management traffic varies with management behavior, the problem of current polling method is inferred from the basis of real data. For the purpose of improving the high performance of shuttle method, we compare and analyze existing

내시경부비동수술후 비음형대, 스펙트럼 및 비·부비동의 용적변화의 분석

김창수,공수근,이현순,조규섭,왕수건,노환중 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

항산화제 섭취가 탈진적 운동 시 장딴지근의 GPx 및 GR의 활성에 미치는 영향

정성태,김연수,이왕록,박익렬,강현주 서울대학교 체육연구소 2001 서울大學校 體育硏究所論集 Vol.22 No.1

The purpose of this present study was to investigate the effect of maximal exercise and a long-term antioxidants supplementation on antioxidant enzyme(GPx and GR) activity of gastrocnemius muscle in rats. Twenty four male Sprague-Dawley rats(4 weeks old) were used as subjects. The subjects were divided into 4 groups such as, CON(n=6 in control group), NAE(n=6 in non antioxidants exercise group), AC(n=6 in antioxidants control group) or AE(n=6 in antioxidants exercise group). Non-antioxidants group(CON & NAE) fed general food(Samyang, Inc., Korea) for 16 weeks. Antioxidants group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(200 lu/kg/day), vitamin C(50 mg/rat/day), vitamin B6(250 ug/kg/day), β carotene(300 mg/kg/day) and selenomethionine(0.1 mg/mg/day), The weight of each subject group was weighed 2-3 times per week. After the diet experiment for 16 weeks, exercise groups(NAE & AE) completed acute treadmill running(speed increased gradually to 25 m/min, 15% uphill grade, ∼50±10 min) until exhaustion. Immediately after exhaustive treadmill exercise, all the subjects were sacrificed for the isolation of gastrocnemius. Then, GPx(Glutathione Peroxidase) and GR(Glutathione Reductase) of the gastrocnemlus muscle were assayed. The protein concentration in the muscle and heart was determined by the Lowry method. After finishing the analysis of antioxidant activity, statistical analysis was carried out by two-way ANOVA and independant t-test. The results of this study can be summarized as follows: Maximal exercise increased the GPx and GR activity of gastrocnemius muscle, Further, a long-term antioxidants supplementation more significantly increased the GPx and GR activity of gastrocnemius muscle than those of non-antioxidants group. In conclusion, the present results clearly show that the exhaustive maximal exercise gave rise to increase oxidative stress which led to oxidative damage so that the antioxidant enzyme activity of gastrocnemius muscle were increased. Further, it has been found that the long-term antioxidants supplementation could decrease oxidative stress in the tissues so that the antioxidant enzyme activity of skeletal muscles was more increased.

중연계 무선네트워크 환경의 도로유지관리계측 시스템 개발에 관한 연구

이상우,송종걸,남왕현,김학수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

Wireless Sensor Networks provide a new paradigm for sensing and disseminating information from various environments, with the potential to serve many and diverse applications. Recent advancement in wireless communications and electronics has enabled the development of low-cost sensor networks. The sensor networks can be used for various application areas. For different application areas, there are different technical issues that researchers are currently resolving. The current state of the art of sensor networks is captured in this article, where solutions are discussed under their related protocol stack layer sections. This article also points out the open research issues and intends to spark new interests and developments in this field. In order to evaluate the application of field monitoring system, lab tests, field test and FEM analysis are conducted. Therefore the accuracy of RFID wireless sensor data is verified.

중연계 무선네트워크 환경의 도로유지관리계측 시스템 개발에 관한 연구

이상우,송종결,남왕현,김학수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Wireless Sensor Networks provide a new paradigm for sensing and disseminating information from various environments, with the potential to serve many and diverse applications. Recent advancement in wireless communications and electronics has enabled the development of low-cost sensor networks. The sensor networks can be used for various application areas. For different application areas, there are different technical issues that researchers are currently resolving. The current state of the art of sensor networks is captured in this article, where solutions are discussed under their related protocol stack layer sections. This article also points out the open research issues and intends to spark new interests and developments in this field. In order to evaluate the application of field monitoring system, lab tests field and FEM analysis are conducted. Therefore the accuracy of RFID wireless sensor data is verified.

후두결핵의 임상적 고찰

이동건,심우영,조재민,박현민,이진춘,왕수건 釜算慶南耳鼻咽喉科學會 1996 임상이비인후과 Vol.7 No.2