Soluble mediators from mesenchymal stem cells suppress T cell proliferation by inducing IL-10

Seung-Ha Yang,Min-Jung Park,Il-Hee Yoon,Su-Young Kim,So-Hee Hong,Jin-Young Shin,Hye-Young Nam,김용희,Bongi Kim,박정규 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.5

Mesenchymal stem cells (MSCs) can inhibit T cell proliferation; however, the underlying mechanisms are not clear. In this study, we investigated the mechanisms of the immunoregulatory activity of MSCs on T cells. Irradiated MSCs co-cultured with either naïve or pre-activated T cells in a mixed lymphocyte reaction (MLR) significantly suppressed T cell proliferation in a dose-dependent manner, irrespective of allogeneic disparity between responders and MSCs. Transwell assays revealed that the suppressive effect was primarily mediated by soluble factors that induced apoptosis. Splenocytes stimulated with alloantigen in the presence of the MSC culture supernatant (CS) produced a significant amount of IL-10, which was attributed to an increase in the number of IL-10 secreting cells, confirmed by an ELISPOT assay. The blockade of IL-10 and IL-10 receptor interaction by anti-IL-10 or anti-IL-10-receptor antibodies abrogated the suppressive capacity of MSC CS, indicating that IL-10 plays a major role in the suppression of T cell proliferation. The addition of 1-methyl-DL-tryptophan (1-MT), an indoleamine 2,3-dioxygenase (IDO) inhibitor, also restored the proliferative capacity of T cells. In conclusion, we demonstrated that soluble mediators from culture supernatant of MSCs could suppress the proliferation of both naïve and pre-activated T cells in which IL-10 and IDO play important roles.

감껍질 열수 및 초임계 유체 추출물의 항아토피 효과

조병옥(Byoung Ok Cho),윤홍화(Hong Hua Yin),방숭주(Chong Zhou Fang),신재영(Jae Young Shin),하혜옥(Hye Ok Ha),김상준(Sang Jun Kim),정승일(Seung Il Jeong),장선일(Seon Il Jang) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.3

본 연구에서는 고종시 감껍질을 열수 추출 및 초임계 유체 추출하여 아토피 피부염 증상 억제 효과를 밝히고, 항염 효능을 나타내는 기능성 소재로서의 이용 가능성을 알아보고자 하였다. 그 결과 육안 평가를 통해 피부의 홍반(erythema), 가려움과 피부의 건조상태(pruritus and dry skin), 부종과 혈종(edema and excoriation), 짓무름(erosion), 그리고 태선화(lichenification)와 같은 아토피 피부염 같은 증상이 AD 모델에서 증가하였지만, SPPE와 PPWE를 투여하였을 경우 완화되는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어난 효과를 나타내었다. 피부 두께와 염증 세포의 침윤은 AD 모델에서 크게 증가하였지만, SPPE와 PPWE를 투여하였을 경우 감소하는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어난 효과를 나타내었다. 혈청 중의 IgE와 IL-4의 수치를 측정한 결과, AD 모델에서 크게 증가하였으나 SPPE와 PPWE를 투여하였을 경우 감소하는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어나게 억제하는 효과를 나타내었다. 또한 RAW264.7 세포에 SPPE를 처리하였을 경우 염증매개 인자인 NO, PGE2, IL-6, IL-1β의 생성량이 유의적의로 감소하였고, PPWE의 경우 NO, PGE2, IL-1β의 생성을 억제한 반면 IL-6 생성 억제에는 영향을 나타내지 않았다. 이러한 염증 매개인자 억제 효능은 SPPE가 PPWE보다 더 뛰어나게 억제하는 것을 확인하였다. 따라서 감껍질 추출물은 아토피 피부염 증상 개선과 염증관련 질환 치료를 위한 기능성 천연물 소재로 유용하게 활용될 수 있을 것으로 판단된다. This study aimed to investigate the anti-atopic effect of hot water (PPWE) and supercritical-carbon dioxide fluid extract of persimmon peels (SPPE) on atopic dermatitis (AD)-like skin lesions in hairless mice. Histological analyses demonstrated that SPPE treatment more strongly inhibited the dermal infiltration of inflammatory cells in AD-like skin lesions than that by PPWE. Compared to PPWE, SPPE significantly decreased the dermatitis clinical score and the epidermal thickness and potently suppressed serum IgE and interleukin (IL)-4 production in hairless mice with AD. Furthermore, compared to PPWE, SPPE potently inhibited the production of nitric oxide, prostaglandin E₂, and proinflammatory cytokines such as IL-6 and IL-1β in lipopolysaccharide-stimulated RAW264.7 macrophages. These results suggested that SPPE exhibited anti-atopic dermatitis activity via the regulation of inflammatory responses.

Effect of Feed Selenium-lysine Supplementation on Milk Compositions and Serum Biochemical Indices in Saanen Dairy Goats

Tae-Il Kim,Dong-Hyun Lim,Tai-Young Hur,Seung-Min Ha,Hyun-Jong Kim,Seong-Min Park,Ji-Hoo Park,Sang-Bum Kim,Ji-Hwan Lee,Hyun-Joo Lim,Jeong-Sung Jung,Ha-Yeon Jeong,Jay Lee,Kwang-Seok Ki,Vijayakumar Mayak 경상대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.4

An experiment was carried out to assess the effect of feed selenium-lysine (Se-Lys) supplementation on milk compositions and serum biochemical indices in Saanen dairy goats in Korea. A total of twelve 36 months old Saanen lactating dairy goats (47±6.21 kg) fed the similar dry matter intake twice a day at 2% of BW (DMI) (10.9% moisture of concentrate and 19% moisture of roughage), milk yield (2.5 kg/d) and parity (2) were randomly selected and subjected for the present study, divided into two groups with six goats in each group. The goats in the control group received rice hulls (10 g/ day) only, and did not receive Se-Lys; goats in the treatment group were fed 0.06 g of Se-Lys with 10 g of rice hulls every day before feeding roughage for six weeks. The milk sample was collected every week, and its compositions were analyzed. The results of the present study showed that there is no significantly increased milk production in Se-Lys treated group goats when compared with control group goats. But, Se-Lys treatment significantly increased the milk protein content (3.98±0.16%), fat (3.72±0.27%), lactose (4.07±0.14%), total solids (12.51±0.28%) and urea (14.42±1.45 mg/dl) content as compared to the control group goats (p<0.05). The somatic cell counts (207,740±28.81 cells/ml) were significantly lower in the Se-Lys treated group than in the control group (p<0.05). Also, the results of the current study showed that supplementation of Se-Lys were significantly decreased the blood biochemical indices of IL-6 (34.34±6.04 pg/ml), TNT-α (0.56±0.22 ng/ml), MDA (5.07±1.03 ng/ml), GPx-1 (9.07±5.17 ng/ml), sCD4 (2.64±1.02 ng/ml) and sCD8 (5.08±2.08 ng/ml) level when compared with without addition of Se-Lys group dairy goats (p<0.05). On the other hand, the selenoprotein P (1,580.18±127.62 ng/ml) level was significantly higher in Se-Lys supplemented group than in the control group (p<0.05). Based on the study results, it was concluded that feed Se-Lys supplementation may improve milk yield with positively improved protein, fat, lactose, total solids, urea content, and biochemical indices without negative effects on milk production traits.

Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol to the Membrane

Kang, Jae Seung,Kim, Ha Na,Jung, Da Jung,Kim, Jee Eun,Mun, Ga Hee,Kim, Yeong Seok,Cho, Daeho,Shin, Dong Hoon,Hwang, Young-Il,Lee, Wang Jae Williams & Wilkins 2007 The Journal of investigative dermatology Vol.127 No.3

It is well known that UVB (290–320 nm) induces inflammation in skin by the transcription and release of cytokines and chemokines from skin keratinocytes. In addition, it is considered that intracellular reactive oxygen species (ROS) plays an important role in UVB-induced inflammatory response in the skin. Therefore, we investigated the effect of vitamin C, a potent antioxidant, on the regulation of UVB-induced skin inflammation via the modulation of chemokines production. Vitamin C uptake into keratinocytes is increased by UVB irradiation in a time- and dose-dependent manner through the translocation of sodium-dependent vitamin C transporter-1 (SVCT-1), a vitamin C-specific transporter, from the cytosol to the membrane. To evaluate the effect of vitamin C on the chemokine mRNA expression, we performed RNase protection assay. As a result, there was a remarkable change in chemokine mRNA expression, especially IL-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, increased IL-8 and MCP-1 mRNA expressions were suppressed by vitamin C treatment. We also confirmed the results of protein levels measured by ELISA. Taken together, vitamin C uptake is increased in UVB-irradiated keratinocytes through the translocation of SVCT-1 and regulates inflammatory response in the skin via the downregulation of IL-8 and MCP-1 production.Journal of Investigative Dermatology (2007) 127, 698–706. doi:10.1038/sj.jid.5700572; published online 28 September 2006

Rosmarinic acid와 luteolin의 항염증에 대한 상승효과

조병옥(Byoung Ok Cho),윤홍화(Hong Hua Yin),방숭주(Chong Zhou Fang),하혜옥(Hye Ok Ha),김상준(Sang Jun Kim),정승일(Seung Il Jeong),장선일(Seon Il Jang) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.1

본 연구에서는 들깨 유래 기능성 물질인 rosmarinic acid (RA)와 luteolin이 RAW264.7 세포에서 항염증작용에 대한 상승 효과가 있는지 알아보고자 하였다. 그 결과 RAW264.7 세포에 RA (50 μM)와 luteolin (1 μM)을 동시에 처리하였을 경우 염증 매개 인자인 NO, iNOS, PGE2, COX-2의 생성을 RA (100 μM)와 luteolin (2 μM)을 각각 처리하였을 때 보다 더 뛰어나게 억제하였다. 또한 RA (50 μM)와 luteolin (1 μM)을 동시에 처리하였을 경우 TNF-α, IL-6, IL-1β 같은 염증성 사이토카인의 생성량을 RA (100 μM)와 luteolin (2 μM)을 각각 처리하였을 때 보다 더 뛰어나게 억제하는 것을 확인하였다. 그리고 RA (50 μM)와 luteolin (1 μM)을 동시에 처리하였을 경우 RA (100 μM)와 luteolin (2 μM)을 각각 처리하였을 때 보다 NF-κB의 subunit인 p65의 translocation과 IκB-α의 degradation을 더 뛰어나게 억제하는 것을 볼 수 있어 두 화합물 간의 상승작용이 뚜렷함을 확인 할 수 있었고, RA와 luteolin 두 화합물을 동시에 처리할 경우 염증관련 질환 치료에 유용하게 활용될 수 있을 것으로 판단된다. The aim of this study was to investigate the synergistic anti-inflammatory effect of rosmarinic acid (RA) and luteolin from perilla (Perilla frutescens L.) leaves in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. A combination of RA and luteolin more strongly inhibited the production of nitric oxide (NO), inducible NOS (iNOS), prostaglandin E2 (PGE2), and COX-2 than higher concentrations of RA or luteolin alone in LPS-stimulated RAW264.7 macrophages. The combined RA and luteolin synergistically inhibited the production of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β), in LPS-stimulated RAW264.7 macrophages. Furthermore, combined RA and luteolin more strongly suppressed NF-κB activation than RA or luteolin alone, by inhibiting the degradation of inhibitor of NF-κB (IκB)-α and nuclear translocation of the p65 subunit of NF-κB in LPS-stimulated RAW264.7 macrophages. Collectively, these results suggest that RA and luteolin in combination exhibit synergistic effects in suppression of LPS-induced inflammation in RAW264.7 macrophages.

자발성 뇌내혈종환자의 치료에서 뇌정위 수술의 역할

하성일,이종수,이승재,박효일 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.2

Computerized tomography(CT) have been available for diagnosis and localization of intracerebral hematoma. CT-guided stereotactic evacuation of spontaneous intracerebral hematoma was performed in 34 cases : 25 basal ganglionic hematomas with or without ventricular perforation, 4 subcortical, 3 thalamic, 1 cerebellar and 1 pontine. The biplane CT image were taken to determine the coordinates of the target point, which was the center of the hematoma. The liquefied or solid portion of the hematoma was aspirated though a burr-hole under local anesthesia. And a silastic tube(3.3㎜ in outer diameter and 2.0㎜ in inner diameter) was then inserted into the center of the hematoma. Immediately after the first trial of hematoma aspiration, urokinase(6000 IU/5㎖ saline) was administered through the tube. Subsequently, aspiration and infusion of urokinase were repeated every 6 or 12 hours until the hematoma was almost completely evacuated. The follow-up results indicate that this procedure was more superior over the conventional craniotomy. This CT-guided stereotactic operation has the following advantages: 1) the procedure is simple and safe : 2) the procedure can be performed under local anesthesia: and 3) the hematoma can be completely drained with the aid of urokinase. This procedure can be used as standard treatment for intracerebral hematoma. We have draw the following conclusions : As a result of the above study. 1) Stereotactic surgery is more effective in the patients with neurologic grade 3 than conservative one(P<0.05). 2) To use of urokinase or perform the early surgery within 24 hours did not seem to be the cause of rebleeding. 3) In the case of pons or cerebellar hemorrhage, stereotactic surgery had definite benefit in the recovery of consciousness even if recovery of motor function was dismal. 4) In patients with spontaneous ICH, the favorable prognostic indicatiors were as follows : the size of hematoma less than 50㎖, no signs of transtentorial herniation and patient's age under 60.

설전절제술후 유리피판술을 이용한 설재건술의 기능적 평가

선동일,김민식,이정학,전진하,송치준,조승호 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.10

韓國 妊婦의 TORC H病原體에 關한 抗體 保有에 關한 硏究

柳承一,金善行,洪性鳳 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

During the recent 15 years, there was a significant changes in the incidence of viral infection in pregnancy. In addition, TORCH agents are the most prevalent microbes among them, and their infections during pregnancy, either primary or recurrent, may affect the feauses in utero and the neonates at the time of delivery. Although we have achieved some progress in controlling them in the view point of prevention and treatment, but it is still insufficient. The purpose of this study is to determine the baseline prevalence of antibodies for TORCH agents during various stage of pregnancy in Seoul, Korea. From 92 asymptomatic pregnant women, 92 single serum sample were taken and examined the antibodies for TORCH agents by ELISA (C.M.V., R.V., H.S.V.) and H.A.I. test (Toxoplasmosis). The followings are the results. 1. The prevalences of antibodies to each of the TORCH agent were 1.08% for toxoplasma, 93.47% for C.M.V., 71.73% for R.V. and 84.74% for H.S.V. 2. The prevalences of antibodies for C.M.V. and R.V. were much higher in the term pregnancy group compared to early pregnancy. This findings suggest some evidence of vulnerability to viral infection during pregnacy. 3. The prevalences of C.M.V., R.V. and H.S.V. antibodies were in parallelism with the increasing age of women. Almost all women over 30-35 years had C.M,V., R.V., H.S.V. antibodies. 4. In multiparous women, the prevalences of antibodies for TORCH agents were higher than that in nulliparous women. 5. In the women with history of previous fetal wastage, the prevalence of TORCH antibodies were higher especially for C.M.V. compared with women without past history of previous fetal wastage.

기포탑내에서 부유물 첨가에 의한 기체체류량과 물질전달계수에 관한 연구

최일곤,한승완,하종열,김상렬 東亞大學校 大學院 1994 大學院論文集 Vol.19 No.-

Gas holdup and volumeteric liquid phase mass transfer coefficient in a bubble column with granular activated carbon were measured according to partcle size and concentration of granular activated carbon and gas velocity. The results of the experiments were as follows ; 1. Gas holdup and volumetric liquid phase mass transfer coefficient increase with increasing the gas velocity. 2. Gas holdup has no effect on concentration of granular activated carbon, but volumetric liquid phase mass transfer coefficient decrease with increasing the concentration. 3. Gas holdup increase with being enlarged particle size of granular activated carbon, but volumetric liquid phase mass transfer cofficient decrease. 4. Correlations among various parameters having an influence on Gas holdup and volumetric liquid phase mass transfer coeffient were expressed by Eq.(9) and Eq.(10) respectively.

土當歸, 日當歸 및 中國當歸가 Hydrocortisone acetate로 유발된 瘀血 病態에 미치는 影響

송승현,서부일,김호경,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : This study was performed to compare the effect of Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix extract on model of blood stasis in rats. Methods : Except for the normal group, hydrocortisone acetate(HA;%mg/kg in ethanol, IM) was injected to induce experimental blood stasis model for 1 weeks and each extract was administrated after lhr following HA injection for lweek. We measured the hematocrit, the platelet count, the prothrombin time, levels of fibrinogen and RBC counts in rat s blood. Results : Angelicae Gigantis Radix, Angelicae Acutilobae Radix group showed significant increase of hematocrit in comparison with that of the control group. Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix group showed significant increase of the platelet count in comparison with that of the control group. Angelicae Gigantis Radix group showed significant decrease of prothrombin time in comparison with that of the control group. Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix group showed significant increase of fibrinogen in comparison with that of the control group. Angelicae Sinensis Radix group showed significant decrease of RBC counts in comparison with that of the control group. Conclusions : Reviewing these experimental results, it appears that Angelicae Gigantis Radix, Angelicae Acutilobae Radix and Angelicae Sinensis Radix have similar pharmaceutical efficacy on model of blood stasis. In particular Angelicae Gigantis Radix showed good efficacy in comparison with Angelicae Acutilobae Radix and Angelicae Sinensis Radix. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.