Identification of the Gene Products Responsible for F Plasmid Partitioning

KIM, SUNG0UK,NAGAI, KAZUO,TAMURA, GAKUZO,YU, JU-HYUN,BOK, SONG-HAE 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4

DNA subfragments, sopA, sopB and sopC which help to maintain the stability of an on C plasmid, were derived from a mini-F plasmid DNA (EcoRI restriction fragment f5) after digestion with restriction endonuclease, and cloned in the vector plasmid pBR322. The recombinant plasmids obtained were introduced into E. coli KY7231 and E. coli CSR603 strains, and proteins specified by the mini-F fragments were analysed by SDS-PAGE. Two proteins encoded by the F fragments were detected, and their molecular weights were 41,000 and 37,000 daltons. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins had been overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of sopA and sopB proteins were 6.6 and 7.0, respectively.

Thermal Decomposition Behavior and Durability Evaluation of Thermotropic Liquid Crystalline Polymers

Shin, Sang-Mi,Kim, Seong-Hun,Song, Jun-Kwang The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.3

The thermal decomposition behavior and degradation characteristics off our different thermotropic liquid crystalline polymers (TLCPs) were studied. The thermal decomposition behavior was determined by means of thermogravimetric analysis (TGA) at different heating rates in nitrogen and air. The order of the thermal stability was as follows: multi-aromatic polyester > hydroxybenzoic acid (HBA)/hydroxynaphthoic acid (HNA) copolyester > HNA/hydroxyl acetaniline (HAA)/terephthalic acid (TA) copolyester > HBA/Poly(ethylene terephthalate) (PET) copolyester. The activation energies of the thermal degradation were calculated by four multiple heating rate methods: Flynn-Wall, Friedman, Kissinger, and Kim-Park. The Flynn-Wall and Kim-Park methods were the most suitable methods to calculate the activation energy. Samples were exposed to an accelerated degradation test (ADT), under fixed conditions of heat ($63{\pm}3^{\circ}C$), humidity ($30{\pm}4%$) and Xenon arc radiation ($1.10\;W/m^2$), and the changes in surface morphology and color difference with time were determined. The TLCPs decomposed, discolored and cracked upon exposure to ultraviolet radiation.

디스크 레이저를 이용한 순 타이타늄의 비드용접시 비드색에 따른 용접특성

김지성(Ji-Sung Kim1),박수한(Su-Han Park),송무근(Moo-Keun Song),김종도(Jong-Do Kim) 한국마린엔지니어링학회 2018 한국마린엔지니어링학회지 Vol.42 No.7

Complication of Intraoperative Radiation Therapy (IORT) in Gastric Cancer

김명세(Myung Se Kim),김성규(Sung Kyu Kim),송선교(Sung Kyo Song),김홍진(Hong Jin Kim),권굉보(Koing Bo Kwan),김흥대(Heung Dae Kim) 대한방사선종양학회 1992 Radiation Oncology Journal Vol.10 No.2

영남대학교 의료원 치료방사선과에서 1988년 6월 15일에 위함 환자의 수술중 방사선치료를 시작한 이래 1992년 8월 30일까지 총 58예에서 시도하여 그중 53예에서 IORT를 실시하였으며, 정기적인 추적검사에서 한명의 국소개발 환자도 보고되지 않고 있다. 출혈(3예), 장관폐쇄(3예), 폐혈증(2예), 골수기능저차(1예)를 포함한 총 9예(17%)의 합병증이 보고되었고, 이중 6예(13%)가 사망하였다. IORT(1500cGy), 외부 방사선치료 (-4500cGy)와 강한 항암제를 병합치료 하였음에도 불구하고 주등(수술과 항암제 치료)의 25.2%, 김등(수술 불가능한 환자에서 항암제 투여)의 18%, 리등(수술)의 18.5%, Kraming등(IORT 2800-3500cGy)의 35.3%에 비해 낮은 합병증을 보여 IORT가 위암의 치료에 공헌할 수 있음을 시사하였다. 그러나 비교적 높은 치사율(11.3%)은 더욱 세심한 수술수기 및 수술 후 환자의 치료가 필요하며 외부 방사선치료와 항암제치료의 적절한 시기 조절 및 치료선량의 가감이 필요할 것으로 생각된다. Local control is the important prognostic factor in cancer treatment because local control decrease the relative risk of metastatic spread and increase distant metastasis free survival. IORT is the modality which could increase local control without increasing complication, combined with curative operation. Eventhough we could achieve significant deacreased local failure by IORT and curative resection, it should not be committed as a main treatment modality without proving acceptable complications. Therapeutic Radiology Department of Yeungnam University Medical Center have tried 58 IORT from June 15, 1988 and performed 53 IORT in patients with gastric cancer. No local failure had been reported including interstinal obstrution, hemorrhage, sepsis, and bone marrow depression. These complications could be comparable to Jo's 25.2% (chemotherapy + operation), Kim's 18% (chemotherapy only in inoperable patients), because our treatment regimen is consisted of IORT (1500cGy), external irradiation (--4500cGy) and extensive chemotherapy (FAM, 5FU + MMC, BACOP). Our data encouraged us to re-inforce further IORT in stomach cancer treatment.

Characterization of cricket song patterns and the behavior observation of Teleogryllus emma (Orthoptera:Grylloidea)

Ji Chang Woo,Kim Nam-Jung,Lee Sang-Guei,Hwang-Yong Kim,Choi Hoon-Sik,Song In-Sun,Ko Jee Sun 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

We analyzed the chirp sound and behavior of Teleogryllus emma with observation system, which was consisted of computer, ccd-camera and microphone. Computational methods of wavelet transformation and Self-Organizing Maps (SOM) were utilized to characterized the chirp sound of insect species for automatic counting in this study. Wavelets were initially applied to feature extraction of the chirp sound. Wavelet coefficients were accordingly calculated based on the basis function (e.g., Morlet). The obtained coefficients were subsequently provided to count number of chirps in each song. Sound structure of insect specimens consisted with long chirp and short chirp and the patterns of song were grouped by frequency of long chirp and short chirp. The song patterns of insect specimens were divided by Self-Organizing Map (SOM) that was used number of chirp as input data. Application of computational methods to automatic detection of chirp sound was further discussed for obtaining objective assessment in behavior science.

초음파 C-scan을 이용한 터빈 블레이드 열차폐코팅의 등온열화에 의한 박리 평가 기법

이호걸(Ho-Girl Lee),김학준(Hak-Joon Kim),송성진(Sung-Jin Song),석창성(Chang-Sung Seok) 한국비파괴검사학회 2016 한국비파괴검사학회지 Vol.36 No.5

가스터빈 블레이드는 고온 고압의 환경 아래 장시간 가동하기 위하여 초합금 모재에 세라믹 코팅으로 이루어진 열차폐코팅(thermal barrier coating, TBC)은 필수요소이다. 하지만 TBC 또한 가스터빈 가동 중 일정 열화온도 및 가동시간에서 top coat의 박리현상이 일어난다. TBC의 박리는 블레이드의 손상과 직결되므로 가스터빈의 안정적인 가동을 위해서 TBC의 박리 평가가 선행되어야 한다. 기존 비파괴평가 기법 연구는 산화알루미늄층(thermally grown oxide, TGO)의 생성 유무 또는 완전 박리의 정성적 평가가 이루어져 왔다. 본 연구에서는 TBC 박리를 정량적으로 평가하기 위해 초음파검사의 C-scan기법을 이용한 TBC의 부분박리손상 map을 구현하였다. 시편들은 1,100˚C로 등온열화하여 각각 열화시간을 변화시킨 시편들을 사용하였다. 단일 탐촉자를 이용한 펄스-에코법으로 C-scan을 수행하였고 TBC 내 부분박리를 검출하기 위해 초음파를 수침법으로 시편에 수직탐상하였다. 그리고 Rogers-Van Buren과 Kim의 이론 반사식을 이용하여 부분박리영역 지름이 1 mm부터 6 mm까지 부분박리지수를 도출했다. 이를 적용하여 각 부분박리지수에 따른 부분박리 손상 map을 영상화하였다. TBC는 열화시간이 증가할수록 부분박리지수에 관계없이 부분박리영역이 모두 증가함을 확인할 수 있었다. 또한 단일 시편 내에서 부분박리지수가 증가할수록 부분박리영역이 감소하는 것을 확인하였다. 부분박리손상 map의 부분박리영역에 따른 분포를 이용하여 TBC의 완전박리 기준과 잔여 수명을 또한 도출할 수 있었다. Thermal barrier coating (TBC) is an essential element consisting of a super-alloy base and ceramic coating designed to achieve long operational time under a high temperature and pressure environment. However, the top coat of TBC can be delaminated at certain temperatures with long operation time. As the delamination of TBC is directly related to the blade damage, the coupling status of the TBC should be assured for reliable operation. Conventional studies of nondestructive evaluation have been made for detecting generation of thermally grown oxide (TGO) or qualitatively evaluating delamination in TBC. In this study, the ultrasonic C-scan method was developed to obtain the damage map inside TBC by estimating the delamination in a quantitative way. All specimens were isothermally degraded at 1,100˚C with different time, having different partial delamination area. To detect partial delamination in TBC, the C-scan was performed by a single transducer using pulse-echo method with normal incidence. Partial delamination coefficients of 1 mm to 6 mm were derived by the proportion of the surface reflection signal and flaw signal which were theoretical signals using Rogers-Van Buren and Kim"s equations. Using the partial delamination coefficients, the partial delamination maps were obtained. Regardless of the partial delamination coefficient, partial delamination area was increased when degradation time was increased in TBC. In addition, a decrease in partial delamination area in each TBC specimen was observed when the partial delamination coefficient was increased. From the portion of the partial delamination maps, the criterion for delamination was derived.

유산소성 운동이 노인들의 신체조성과 등속성 슬관절 근력 및 요부근력에 미치는 영향

한종우(Jong Woo Han),이상석(Sang Sug Lee),송분도(Bun Do Song),지용석(Yong Suk Ji),김난수(Nan Su Kim),서경호(Kyeong Ho Seo) 한국사회체육학회 2000 한국사회체육학회지 Vol.13 No.-

This study was designed to investigate the effect of a year aerobic exercise training on body composition, isokinetic knee and trunk muscular strength of elders. The subjects for this study were eighty healthy elderly people who were members of seniors tower at Song-Do Hospital and didn`t have any history of cardiorepiratory disease. Subjects have been trained three times a week for a year. The exercise program was made up of a 15-minute warm-up, cool-down. Stretching was performed every eight minutes for the prevention of injuries which could occur during the main exercise and quick recovery of the used muscle. Aerobic training was performed for each 15-20minutes using the treadmill and cycle ergometer at the intensity of 50-70% HRmax. For the data, all the measurements were represented by mean and standard deviation using SAS package(version 6.12). Statistical techniques for data analysis were paired t-test to determine the difference between pre and post exercise program. The 5% level of significance was used as the critical level for acceptance of hypotheses for the study. The following results were obtained from this study. 1. There is a statistically significant difference in the VO_2max, fat mass, lean mass and %body fat both old men and women after a year training. 2. There is not a statistically significant difference in the weight, WHR and thigh circumference both old men and women after a year training. 3. There is a statistically significant difference in the right knee flexor/extensor peak torque, peak torque % body weight, total work and total work % body weight both old men and women after a year training. 4. There is a statistically significant difference in the left knee flexor/extensor peak torque, peak torque % body weight, total work and total work % body weight both old men and women after a year training.

동씨침(董氏鍼)의 경항통(頸項痛) 치료(治療)에 관한 임상적(臨床的) 고찰(考察)

이채우,조태성,박인범,김상우,안창범,송춘호,윤현민,장경전,Lee, Chae-woo,Cho, Tai-Sung,Park, In-beohm,Kim, Sang-woo,Ahn, Chang-beohm,Song, Choon-ho,Youn, Hyoun-min,Jang, Kyung-jeon 대한침구의학회 2003 대한침구의학회지 Vol.20 No.2

Objective : The purpose of this study was to evidence the effect of Dong-Shi Acupuncture by improving degree of forty four patients who have received a Dong-Shi Acupuncture therapy at Dong Eui oriental hospital OPD and chiefly complained for neck pain and related symptoms. Materials and Methods: Forty four patients were diagnosed with physical inspection and radiography, and investigated into the effect of treatment sharing before treatment and after 2 weeks and 4 weeks treatment by Dr. Kim's method. Results and Conclusions: The conclusion of this study was that improving degree of forty four patients who have received a Dong-Shi acupuncture therapy for all items and excellence of the Dong-Shi Acupuncture was evidenced.

Proportion And Characteristics Of Transient Nodules In A Retrospective Analysis Of Pulmonary Nodules

( Jin Yeong Yu ),( Su Young Chi ),( Hee Jung Ban ),( Yong Soo Kwon ),( In Jae Oh ),( Kyu Sik Kim ),( Yu Il Kim1 ),( Sung Chul Lim ),( Song Choi ),( Yun Hyeon Kim ),( Young Chul Kim ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

Effects of Natural Zeolite on Mechanical and Thermal Properties of Epoxy Composite System

Song, Young Wook,Kim, Sang Wook 서울시립대학교 산업기술연구소 1996 산업기술연구소논문집 Vol.4 No.-

The natural zeolite was introduced to diglycidyl ether of bisphenol A(DGEBA)/4,4'-methylene dianiline(MDA)/hydroquinone-phenyl glycidyl ether (HQ-PGE) system to modify mechanical and thermal properties of epoxy resin as an inorganic filler. In mechanical properties, the tensile strength of a system with 10 phr of natural zeolite decreased about 17 % than that of a system without natural zeolite. Also, the impact strength of a system with the natural zeolite decreased than that of the system without natural zeolite. Both properties decreased with the increment of natural zeolite contents. In case of thermal properties, glass transition temperature(Tg) and decomposition temperature(Td) of the composites system were seldom affected by natural zeolite and its contents.