An Experimental Study for a Batch PMMA Polymerization Reactor System

Rho, H.-J 한국화학공학회 1996 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.34 No.1

麻藥 및 向精神性 醫藥品의 標準品 製造에 關한 硏究(Ⅱ) : 韓國産大麻의 性分硏究 및 標準品 製造에 關한 硏究 Studies on the components and preparation of reference standard from Korean Cannabis

元道喜,姜信晶,崔燉雄,李秀庭,朴相勇,韓貞愛,盧榮洙 국립보건연구원 1992 국립보건원보 Vol.29 No.2

Antiviral activity of Alpinia katsumadai extracts against rotaviruses

Kim, H.H.,Kwon, H.J.,Ryu, Y.B.,Chang, J.S.,Cho, K.O.,Hosmillo, M.D.T.,Rho, M.C.,Park, S.J.,Lee, W.S. British Veterinary Association [etc] ; W.B. Saunde 2012 Research in veterinary science Vol.92 No.2

In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H<SUB>2</SUB>O layer), AK-5 (40% methanol fraction), and AK-9-11 (H<SUB>2</SUB>O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC<SUB>50</SUB> values ranging from 0.7+/-0.4 to 33.7+/-6.5μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC<SUB>50</SUB> values of 8.4+/-2.2μg/mL, 6.5+/-0.8μg/mL and 8.4+/-5.0μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.

Valproate 장용성 제제의 용출률과 함량 균일성 평가

김명진,정양희,김재연,조영환,노환성 한국병원약사회 1999 병원약사회지 Vol.16 No.4

Valproate is an effective epileptic drug to control partial seizure and infantile spasm specially but the therapeutic range is narrow, so it is objective of TDM. The efficacy and safety of drug therapy depends on the bioavailability and the dissolution and content uniformity tests are very important procedures for the controlling of medication bioavailability. This study selected three enteric-coated valproate tablet that is administered to patients 2 or 3 times daily in Asan Medical Center and evaluated the bioavailability by the dissolution test and content uniformity test, attempted to estimate that the release of valproate in vivo is suitable for present drug therapy or not. The dissolution test was performed in the solution Ⅰ and the solution Ⅱ that was made by K.P. 7 for three hours and all sample were measured by HPLC. The results were as followed : (1) The mean dissolution amount in the solution I was higher in order of three different brands B (14.9%) > A (7.52%) > C (2.45%) (2) The mean dissolution amount in the solution Ⅱ was higher in order of three different brands B (114%) > A (102%) > C (22.2%). (3) The content uniformity was higher in order to B (117.6%) > A (110.3%) > C (85.8%). In conclusion, the dissolution of A and B in vivo seems to be suitable for present drug therapy but the release of C in vivo seems to be not.

BELLE TOF 검출기의 특성 연구

남지우,김동욱,배미영,박강순,김정현,노상률,최영일,김희종,장동욱,최소명,권영준,Hikita, S.,Yu, C.,Yoshimura, Y.,Kichimi, H. 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

We investigated the properties of BELLE Time Of Flight(TOF) counter using the data taken in Jan 98 beam test at Pi2 beam line at KEK 12 GeV proton synchrotron. Charge distributions from readout PMT were analysed in comparison with dE/dx distributions predicted by GEANT full simulation. Experimental setup of the beam test is described, then procedures of data analysis, Monte Carlo simulation and analysis results are also described.

반도체 검출기를 이용한 Hybrid 전치증폭기형 전자식 개인선량계 개발

이봉재,김봉환,장시영,김종수,노승용 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.1

반도체 검출기를 이용하여 Hybrid 전치증폭기를 갖는 전자식 개인선량계를 설계 제작하고, 방사선 검출 반응특성과 기계적 및 주변 환경 요건에 따른 각종 성능을 평가하였다. ^137Cs 감마 방사선을 이용한 방사선 조사 시험결과 감도는 3.8 cps/Gy·h^-1이고, 10 uSv ∼ 4 Sv 범위의 선량당량 조사시 선량계의 지시값은 오차 8%이하에서 선형성이 잘 유지되었다. 방향의존성은 ±60˚이내에서 4%이하이며 ISO 기준선장에 의한 에너지 반응역역은 60 ∼ 1,250 keV로 나타났으며 IEC, 61526에 의한 선량계의 성능검사시 9개 영역의 시험항목에 대한 국제기준을 만족하였다. An electronic personal dosimeter(EPD) with hybrid type preamplifier adopting a semiconductor detector as a radiation detector has been developed, manufactured and test-evaluated. The radiation detection characteristics of this EPD has been performance-tested by using a reference photon radiation field. After several test-irradiations to a ^137Cs gamma radiation source the radiation detection sensitivity of this EPD appeared to be 3.8 cps/Gy·h^-1, The linearity of radiation response was kept within 8% of the dose equivalent ranges of 10 uSv ∼ 4 Sv and the angular dependence was under less than 4% in angles of ±60˚. It was confirmed that the energy response range was in 60 ∼ 1,250 keV given in the ISO standard. This EPD satisfied the international criteria for the EPD in the mechanical and the environmental performance test for 9 test categories according to IEC 61526.

忠北道內 葉煙草 病蟲害의 發生 및 被害狀況調査

魯斗鉉,李重基,朴然圭,安長憲,盧載榮,朴相一 충북대학교 엽연초연구소 1971 煙草硏究 Vol.1 No.-

a) This investigation was carried out in Chung-Buk province in order to obtain the basic data for divising an effective measure of controling tobacco plant pests and insects, and for establishing the cropping system to produce better and more tobacco by thoroughly inquiring into the out break of tobacco plant pests and insects and the suffering conditions. b) Out-break rates of the main diseases and insects. As for the disease, obvious differences were shown among the kinds of disease and among the districts. Rates of out break of tobacco plant diseases were in the following order : brown spot, granville wile and virus for kinds of diseases : and Chungju-Miwon, Eumsong Jinchon, Choongju-Jechon, Poeun, Okchon, and Jeung pyong for districts. Significant differences were recognized between the region of Chongju-Miwon and the region of Poeun, Okchon, Jeungpyong, and between the region of Jinchon-Eumsong, and the region of Okchon, Jeung pyong, while the differences among other regions were insignificant. As for the insects, outbreak rate of tobacco moths amounted to 50. 2% of tobacco plants, and differences were to be seen among regions. There were differences among the region of Chung ju-Miwon, Jinchon-Eumsung and four other regions while no differences were shown among the regions of Okchon, Poeun, Jeung pyong-Choonfiu and Jechon c) Rate of injuries by those plant pests and insects were as follows: i) Diseases : quantity‥‥‥‥‥‥‥‥‥11.52% quality ‥‥‥‥‥‥‥‥‥18.42% ii) Isects : quantity‥‥‥‥‥‥‥‥‥‥‥1.53% quality‥‥‥‥‥‥‥‥‥‥‥‥1.64% Over all rates of injuries amounted to 13.05% in yields and to 20.06% in quality. d) Tendencies of outbreak and rates of injuries by each plant and insects were as follows: 1) Diseases a) Brown spot Out break-rate of brown spot was the highest, 37.8% among all. No differeances were shown by the number of successive cropping years of tobacco plants on the same land. However, highly significant differences were found among different districts. Districts of Chonaju-Miwon showed highest rate of outbreak 79.5%, As to the rates of injuries in yield by this disease, differences were shown among districts and among kinds of leaves. And same tendencies were to be seen for the rates of injuries in qualities. Especially there were highly significant defferences among kinds of leaves. Rate of injuries both in yield and in quality was highest in medium leaves and in the order of main leaves, low leaves and high leaves. Some of the rates of injuries in different districts are shown below : ◁표 삽입▷ (원문을 참조하세요) The average rails of injuries caused by this disease (Brown Spot) were 1.00% in yield and 1.85% in quality. b) Virus disease Average rate of outbreak of virus was 15.2%, next to brownspot, and was not related with the number of successive cropping years of tobacco on the same land. But each district showed different infection rate of virus; for instance, the outbreak-rate of virus in Jinchon Eumsong district amounted to 29.7%, which was the highest. Rate of injuries in yield by virus showed no obvious difference among districts but there were highly significant differences according to the degrees of infection: highest rate of injuries occured at outbreak index "5." Rates of injuries in quality showed highly significant differences among districts and degrees of infection: Several injuries were found in Jinchon Eumsong district, being 15.57% and at outbreak index "5" The average rates of injuries by this disease were 4.18%, in yield and 8.25% in quality injuries in yield was next to the highest caused by granville wit and injuries in qualities was the highest among the injuries caused by other diseasess and insects investigated. c) Granville wilt It was only granville wilt among the diseases which were investigated that showed sign ificant difference of outbreak rate and of rate of injuries in yield owing to the number of successive years of cropping tobacco on the same land differences in rates of injuries were insignificant in each districts, but the more the years of continious cropping of tobacco the more the outbreak rate and loss in yield were observed. Outbreak rate of this disease was 9.9% and highest rate of injuries in yield was 9.9% found on the land of 20 years successive cropping of tobacco. Average rate of injuries caused by granville wilt were 5.95% in yield, the highest among the diseases and insects, and 7.91% in quality, the next the highest injuries caused by virus. 2. Injuries caused by insects: a) Tobacco moths and Tobacco bug. Outbreak rate of mouths was 50.2% showing no difference owing to the number of successive cropping years of tobacco on the same land. But high differences in outbreak rate were found among districts : for instance, in Chongju-Miwon district, outbreak-rate amounted to 78% which was the highest percentage. Rates of injuries in both yield and quality showed great differences among districts and kinds of leaves. Greatest injuries occured in Chongju-Miwon district being 2.55% in yield and 2.59% in quality. Main leaves suffered the most injuries and next were in the order of medium leaves, high leaves and low leaves. Outbreak rate of tobacco bug was 2.9%, and the rate of injuries was 0.02%, only in quality, very slight, Average rates of loss caused by insects in the whole districts were 1.45% in yield and 1.55% in quality. b) Nematode. This survey was carried out in order to reveal tendency of the inhabitation density of nematode according to successive cropping of tobacco plants on the same land. Inhabitation density of nematode was found to be increasing remarkably with the years of successive of tobacco. In course of 5 to 6 years of successive cropping of tobacco plants on the same land, inhabitation density increased gradually and reached the maximum density at 11 to 20 years of successive cropping. Among districts, difference was found between the new production area and the old production area. As describe above, out break of granville wilt of tobacco plants increased with the number of successive cropping years. And with the same number of successive cropping years, outbreak of granville wilt increased with the inhabitation density of nematode. c) The effect of brown spot, anthracnose, wild fire etc, upon the yield and quality of tobacco plants, which have not studied in this investigation will be studied later. And the later study will also include concrete survey on the time and outbreak conditions of tobacco disease and insects, countermeasures against successive cropping of tobacco, and relation between inhabitation density of nematode outbreak diseases, etc.

Microsatellite 를 이용한 돼지 Yorkshire 품종의 일당중체량 연관 DNA Marker 개발

송영민,김철욱,조광근,박외선,권은정,이정규,백동훈,최진성,노정만,홍연희 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to develop DNA markers related with average daily gain (ADG) using 27 pigs. Animals were divided into two groups: low ADG group had 11 pigs with 1.5 SD and high ADG group was consisted of 16 pigs with +1.5 SD. Initially, we choose and tested 78 microsatellites which could contain potential DNA markers for ADG. Part of the microsatellites were amplified by the polymerase chain reaction (PCR) and then electrophoresed on a agarose gel followed by the allelic, genotypic and statistical analysis. According to the alleic frequency analysis, 21 DNA polymorphisms were detected from 17 microsatellites and appeared to be associated with ADG (P$lt;0.05). From the genotypic frequency analysis, 14 DNA polymorphisms were found from II microsatellites and were associated with ADG. All pigs in the low ADG group had a 135:144 hetero type for the SWR308 marker, whereas all pigs in the high ADG group had a 144:144 homo type. This and possibly other selected markers could be useful for the selecting young pigs with an excellent growth potential.

Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)

Ock, S.A.,Kwack, D.O.,Cho, S.R.,Cho, S.K.,Yeao, E.H.,Yoo, J.G.,Lee, Y.R.,Lee, H.J.,Choe, S.Y.,Rho, G.J. 韓國受精卵移植學會 2002 한국동물생명공학회지 Vol.17 No.1

Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.

開心術 70례 보고 ―1985년도―

노중기,남충희,이길노,이철세,강창희 순천향대학교 1986 논문집 Vol.9 No.1

Seventy cases of the open heart surgery were performed in the Department of Thoracic and Cardiovascular surgery of Soonchunhyang University in 1985. Of them, 52 cases were congenital anomalies which were classified as 43 acyanotic patients and 9 cyanotic patients, and 18 cases were aquired valvular diseases which contained 17 rheumatic valvular diseases and 1 mitral valve prolaps syndrome . The most common anomaly of acyanotic congenital group was VSD(22cases) and the one in cyanotic group was TOF(5 cases). In 18 cases of the acquired valvular diseases, open mitral commissuratomy was done in 2 patients, tissue valve replacement(Carpentier-Edwards Bioprosthesis) in 9, mechanical valve replacement(Medtronic-Hall) in 6, and mitral valvulo-plasty with Carpentier ring in 1 patients. The most frequent complications during postoperative course were arrhythmia and low cardiac output syndrome, Perioperative mortality rate was 5.7%.