Collagen으로 유발된 생쥐의 관절염에 대한 당귀사역가오수유생강탕(當歸四逆加吳茱萸生薑湯)의 효과

김민균 ( Min Kyun Kim ),오민석 ( Min Seok Oh ) 한방재활의학과학회 2011 한방재활의학과학회지 Vol.21 No.2

Objectives: This study was carried out to know the effects of Danggwisayeokgaohsuyusaenggang-tang(hereinafter referred to DST) on arthritis induced by collagen on DBA/1 OlaHsd mice. Methods: For this purpose, DST was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, high performance liquid chromatograph(HPLC) analysis, arthritis index, value of immunocyte in draining lymph node and paw joint, cytokine were measured in vivo. Results: 1. The cytotoxicity against human fibroblast cells(hFCs) was not measured in any concentration. 2. In HPLC analysis, There are high peak patterns at 8 minute(min), 12 min, 35 min, 45 min. 3. The arthritis index was decreased significantly. 4. The degree of arthritis induced damage of joint of DST group is slight compared with control group in histopathologic observation(Hematoxylin and eosin stain(H&E), Masson`s trichrome(M-T) staining). 5. In total cell counts of draining lymph node(DLN) and paw joint, the cells in DLN decreased significantly on DST 200 mg/kg and the cells in paw joint decreased significantly on 200 mg/kg and 50 mg/kg. 6. In DLN, CD4+/CD25+, CD3+/CD69+, major histocompatibility complex(MHC) class-II/CD11c+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg, CD3+/CD8+ cells decreased significantly on DST 200 mg/kg, CD4+, CD3+/CD44+ cells decreased. 7. In paw joints, CD4+, CD11b+/Gr-1+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg. 8. In joints, levels of IL-1β, IL-6, TNF-α, cyclo-oxygenase-2(COX-2), NOS-II were decreased on DST 200 mg/kg and DST 50 mg/kg. 9. In analysing of cytokine in CD3/CD28 activated spleen, IL-17 was decreased significantly, IL-4 was increased significantly and INF-γ was decreased on DST 200 mg/kg. 10. In analysing of cytokine in collagen activated spleen, IL-17 were decreased significantly, IL-4 was increased significantly. Conclusions :This results demonstrated that DST suppressed the inflammatory progression of collagen-induced arthritis(CIA) mice and supported further studies are required to survey continuously in looking for the effective substance and mechanism in the future.

Determining the Optimal Cooking Time for Cooking Loss, Shear Force, and Off-Odor Reduction of Pork Large Intestines

Sung-Su Kim,Yee Eun Lee,ho Hyun Kim,Joong-Seok Min,Dong Gyun Yim,Cheorun Jo 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

The objective of this study was to determine the optimal cooking time by considering the cooking loss, shear force, and off-odor reduction of pork large intestines. Commercial pork large intestines were purchased, quartered perpendicularly, and cooked in boiling water for 40, 120, 180, and 240 min. Cooking loss of the samples increased after 240 min of cooking (10.92, p<0.05) while shear force value was lower at 240 min (4.45) compared to that at other cooking times (p<0.001). The amount of major volatile organic compounds showed a decreasing trend with increasing cooking time. In particular, the amount of methyl pentanoate (17,528.71) and methyl isobutyrate (812.51), compounds with a relatively low odor threshold, decreased significantly after 120 min of cooking and no change was observed thereafter (p<0.05). In addition, the amount of 2-pentanol (3,785.65) and 1-propanol (622.26), possibly produced by lipid oxidation, significantly decreased at the same cooking time (p<0.001). In the principal component analysis, only the 40 min cooking time was significantly different from other cooking time by high amounts of 1-propanol, 2-pentanol, and methyl isobutyrate. In conclusion, in the present study, the optimal cooking time for pork large intestines was 120 min in terms of off-odor reduction, cooking loss, and shear force.

자바리, Epinephelus bruneus의 난 발생과 부화에 미치는 수온의 영향

양문호,최영웅,정민민,구학동,오봉세,문태석,이창훈,김경민,한석중,Yang, Moon-Ho,Choi, Young-Ung,Jung, Min-Min,Ku, Hag-Dong,Oh, Bong-Sae,Moon, Tae-Seok,Lee, Chang-Hoon,Kim, Kyong-Min,Han, Seock-Jung 한국발생생물학회 2007 발생과 생식 Vol.11 No.2

This study examined temperature effect in egg development and hatching of longtooth grouper, Epinephelus bruneus. Fertilized embryos was not growth after morula stage at $15^{\circ}C$, at 18, 21, 24 and $27^{\circ}C$, the required time from fertilized embryos to hatching were 70 h. 30 min., 44 h. 10 min., 29 h. 10 min. and 24 h. 30 min., respectively. The hatching rates at $24^{\circ}C$ were higher than the other conditions and the hatching was not occurred at $15^{\circ}C$. These results suggest that the water temperature range of egg development and hatching was $18{\sim}27^{\circ}C$. 이 연구는 실내 사육수조에서 자연산란 후 수정된 난을 대상으로 수온에 따른 난 발생속도와 부화율을 조사하였다. 부화에 이르기까지 각 수온조건에서 소요된 시간은 $15^{\circ}C$에서 상실기 이후 발생이 진행되지 않았고, 18, 21, 24, $27^{\circ}C$에서 각각 70시간 30분, 44시간 10분, 29시간 10분 그리고 24시간 30분이 소요되었다. 부화율은 $15^{\circ}C$에서 0%였고, 18, 21, 24 그리고 $27^{\circ}C$에서 각각 $8.3{\pm}1.2%$, $18.0{\pm}6.2%$, $24.0{\pm}4.0%$ 그리고 $17.0{\pm}7.2%$로 뚜렷한 차이 없이 $24^{\circ}C$에서 다소 높았고 21와 $27^{\circ}C$에서 비슷한 경향이었으며 $18^{\circ}C$에서 가장 낮게 나타났다. 이러한 결과에서 자바리의 난발생 수온범위는 $18{\sim}27^{\circ}C$로 제안된다.

The Theology of Images in the Reformation

Min?Seok Kim 한국기독교학회 2016 한국기독교신학논총 Vol.101 No.-

교회 역사는 건축, 음악, 그림, 캘리그라피, 드라마, 조각, 영화, 스테인드글라스 등과 같은 예술적이고 기술적인 시각 매체 혹은 수단들을 통해서도 복음이 사람들에게 전달되어 왔음을 알려주고 있다. 더 나아가 현대 사회에서는 시각 이미지의 긍정적 가치와 의미가 더욱 대두되고 있다. 그 결과로 현대 교회들은 영상, 그림, 그리고 드라마와 같은 시각 이미지들을 설교와 예배에 대한 보조 도구들로 사용하기도 한다. 이러한 상황은 개신교 전통의 현대교회들을 위해서 개신교 신학 안에서 이미지에 대한 긍정적인 신학의 추구와 발전을 요구하고 있다. 그러나 많은 개신교회와 개신교 목회자들이 개신교 신학에는 시각 이미지에 대한 부정적 관점만 있을 뿐이라고 추정하는 것처럼 보인다. 그리고 이러한 단정적 추론은 개신 교회에서 경건과 예배를 위해 사용되는 시각 이미지에 대해 긍정적인 신학을 추구하고 찾는 데 걸림돌로 작용하고 있다. 이러한 상황 속에서 본 연구는 개신교 신학들 중에서도 이미지에 대한 긍정적 관점이 있는지를 찾고자 한다. 이 목적을 수행하기 위해 본 논문은 먼저 중세 후기의 이미지 오용을 다루고, 그 후에 세명의 종교 개혁자들(안드레아스 보덴슈타인 폰 칼슈타트, 마르틴 루터, 그리고 장 칼뱅)의 관점을 분석한다. 칼슈타트는 종교 개혁 진영에서 맨 처음으로 급진적 성상 파괴 운동을 공식적으로 시작했다. 그리고 루터와 칼뱅은 여전히 개신교 전통의 현대 교회들에 영향력을 행사하고 있다. 필자는 이러한 이유 때문에 16세기의 종교 개혁가들 중에 위에 언급된 세 명의 종교 개혁자를 선택했다. Church history has shown that the Gospel has been communicated and transmitted to people through artistic and technological media or ways such as architecture, music, paintings, calligraphy, drama, sculpture, movies, stained glass, etc. Moreover, in modern society, the visual image is increasing in positive value and meaning so that modern churches have used visual images such as films, pictures, and dramas as aids for preaching or worship. This contemporary circumstance calls for the pursuit and development of the positive theology of the image in Protestantism for our contemporary Protestant churches. However, the majority of Protestant churches and minsters seems to assume that there is only a negative perspective toward visual images in the theology of the Reformation. This assumption presents an obstacle to pursuing and finding a positive theology of images in order to use visual images for devotion and worship in Protestantism. With this context as a foundation, the objective of this study is to find a positive perspective of visual images in Protestant theologies. To this end, this article addresses the abuse of images in the late medieval period and then analyses three reformers’ perspective of image: Andreas Bodenstein von Karlstadt, Martin Luther, and John Calvin. The first was the official initiator of the iconoclasm in the sixteenth–century Reformation and the latter two reformers have still exerted influence on our contemporary Protestant churches. This is the reason why I choose these three among the sixteenth–century reformers.

Antioxidant Activity and Whitening Effect of Lindera erythrocarpa Extract

Min ?Chul Kang,Ryeo Kyeoung Ko,Ju-Yeop Lee,Haeng-Bum Kim,Bong-Seok Kim,Jong-Heon Han,Seong-Hyun An,Gi-Ok Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

Evaluation of High Power Light Emitting Diodes (HPLEDs) as Potential Attractants for Adult Spodoptera exigua (Hubner) (Lepidoptera Noctuidae)

( Min Seok Oh ),( Chi Hoon Lee ),( Sang Guei Lee ),( Hoi Seon Lee ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3

To evaluate high-power light-emitting diodes (HPLEDs) as potential attractants for Spodoptera exigua adults, attractiveness of specific wavelengths, illuminance intensity, and light-exposure times were investigated and compared to that of fluorescent light. The white light (40 lux treatment) attracted significantly more S. exigua than other attractants. The optimal light-exposure time, based on the highest attraction rate, was 60 min. Evaluation of attraction and repellent rates under optimal conditions showed the white HPLED had the highest attraction rate (91.1%), and the red HPLED had the highest repellence rate (33.3%). Based on relative efficiency values, the white HPLED was about 9.14 times as efficient as the fluorescent light. These data clearly showed that S. exigua showed the greatest attraction to the white HPLED at 40 lux intensity and 60 min light-exposure time.

Min-Hash를 이용한 효율적인 대용량 그래프 클러스터링 기법

이석주(Seok-Joo Lee),민준기(Jun-Ki Min) Korean Institute of Information Scientists and Eng 2016 정보과학회논문지 Vol.43 No.3

Graph clustering is widely used to analyze a graph and identify the properties of a graph by generating clusters consisting of similar vertices. Recently, large graph data is generated in diverse applications such as Social Network Services (SNS), the World Wide Web (WWW), and telephone networks. Therefore, the importance of graph clustering algorithms that process large graph data efficiently becomes increased. In this paper, we propose an effective clustering algorithm which generates clusters for large graph data efficiently. Our proposed algorithm effectively estimates similarities between clusters in graph data using Min-Hash and constructs clusters according to the computed similarities. In our experiment with real-world data sets, we demonstrate the efficiency of our proposed algorithm by comparing with existing algorithms.

Evaluation of High Power Light Emitting Diodes (HPLEDs) as Potential Attractants for Adult Spodoptera exigua (Hübner)

Min-Seok Oh,Sang-Guei Lee,Ju-Hyun Jeon,Hoi-Seon Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

To response evaluation of high power light emitting diodes (HPLEDs) as potential attractants to the Spodoptera exigua adults, we investigated the attractiveness of specific wavelength, illuminance intensity and light-exposure time, and compared them to the fluorescent. The all light treatments with the 40 lux intensity attracted the significantly highest number of S. exigua. The optimal light-exposure time exhibited the highest attraction rate at the 60 min. When the attraction and repellent rate in the optimal conditions to the S. exigua was surveyed, the white HPLED exhibited the highest attraction rate (91.1%), whereas the red HPLED exhibited the most repellent rate (33.3%). When evatuated of illuminance efficiency with fluorescent as control, white and red HPLED were found to be 9.14 and 10.34 times more efficient than fluorescent. These data clearly show that both the 40 lux intensity and the 60 min light-exposure time by using the white HPLED was the most suitable for attraction of the S. exigua.

농업 부산물 바이오매스 특성에 따른 반탄화 공정 최적화: 1. 질량감소 예측 모델 개발

김석준 ( Seok Jun Kim ),오광철 ( Kwang Cheol Oh ),박선용 ( Sun Young Park ),조라훈 ( La Hoon Cho ),김민준 ( Min Jun Kim ),이충건 ( Chung Geon Lee ),정인선 ( In Seon Jeong ),전영광 ( Yeong Kwang Jeon ),김대현 ( Dae Hyun Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.2

본 연구에서는 국내에서 버려지고 있는 농업부산물의 에너지로서 이용 가능성이 분석되었다. 농업부산물 바이오매스는 화석연료와 동일하게 활용가능하며 지속적으로 생산되는 에너지원이다. 하지만 높은 수분 함량에 따른 보관문제, 화석연료와 비교하여 낮은 발열량등의 단점을 지니고 있어 전처리공정이 요구된다. 따라서 효율적인 활용을 위한 전처리 공정으로 반탄화가 선정되었다. 반탄화공정은 수분제거를 통해 에너지가 증가되어 저장·운송에 이점으로 인하여 최종적인 에너지 가치가 증대된다. 실험은 문헌탐색을 통한 반탄화공정 적용 가능 농업부산물(고추대)과 실험조건(200, 230, 270C, 10, 20, 30, 40 min)이 선정되었으며, 1차원 시뮬레이션을 통한 분석이 이루어졌다. 이때 반탄화 실험을 통하여 발생되는 질량감소량과 발열량 변화량은 높은 상관관계(r²=0.9528)를 가지고 있으며 본 연구에서는 반탄화공정의 질량감소량 예측 모델개발이 수행되었다. 질량감소량 분석을 위하여 열 중량 분석(Thermo Gravimetric Analyzer, TGA)이 수행되었으며 승온속도(5, 7.5, 15, 22.5, 30, 37.5°C min^-1)에 따른 반응속도상수가 도출되었다. 1차원 시뮬레이션 분석을 통하여 바이오매스 내부 온도를 예측하였으며 앞서 도출된 반응속도 상수를 이용하여 질량감소량이 예측되었다. 질량감소량 실험 및 시뮬레이션 결과 평균 r²=0.97로 상관관계를 나타내고 있으며 공정온도별 평균제곱근오차(Root Mean Square Error, RMSE)가 200°C-0.0165, 230°C-0.0191, 270°C-0.0207로 나타났다. 공정온도 증가에 따라 정확도가 감소되었으며, 이는 고온의 실험조건에서 발생되는 비응축가스와 온도변화에 의한 열분해 특성에 기인한 것으로 판단된다. 최종적으로 더 나아가서 질량감소 모델을 통한 발열량 예측을 위한 분석이 이루어져야한다.

Methylmethacrylate Monomer의 가토 신장기능에 미치는 영향 : Effects of Methylmethacrylate Monomer on the Renal Function in Rabbits

김민형,장석종 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

Relating to toxicity of methylmethacrylate monomer, there are many evidences of pathological changes, ie. parenchymal degeneration in pulmonary, hepatic, and renal tissue of animal with high dosage. But the reports about the effects of methylmethacrylate monomer on renal function are rare. Therefore the author intended to investigate the effects of methylmethacrylate monomer on parameters of renal function. Experimental dosages of methylmethacrylate monomer were 0.1㎕/min and 0.25㎕/min, of which didn't cause the arterial hypotension. The infusion was perfermed at a rate of 30 ml/hr for 1 hour. During the one hour periods of administration of methylmethacrylate and recovery proces blood and urine samples were collected and analyzed. Plasma clearances of substances various were obtained by the measuring the plasma and urine concentrations of inulin, PAH, osmolarlty sodium and potassium. The results were summarized as follows. 1. Glomerular filtration rate and renal plasma flow were not affected at a dose of but tended to decrease at a dose of 0.25ul/min of methylmethacrylate monomer. 2. Urine flow was reduced significantly (p<0.05) by methylmethacrylate monomer. 3. Urine osmolarity was increased significantly (p<0.05) by methylmethacrylate monomer. 4. Negative free water clearance was increased significantly (p<0.05) by methylmethacrylate monomer. 5. Osrnolar clearance and sodium and potassium reabsorption rate were not affected significantly by methylmethacrylate monomer. From the above results, it was suggests that methylmethacrylate monomer caused the reduction of urine flow through enhancement of tubular free water reabsorption and partially affecting the GFR and RPF.