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Insecticidal activity of Ruta chalepensis leaf-derived component against Sitophilus zeamais
Ju-Hyun Jeon,Geon-Hwi Lee,Sang-Guei Lee,Hoi-Seon Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Insecticidal activity of active component isolated from Ruta chalepensis leaves was examined against maize weevil, Sitophilus zeamais and compared with two different bioassay system, such as direct contact and fumigant method. The methanol extract of R. chalepensis leaves had strongly (+++) insecticidal activity at 50 mg/disk against S. zeamais. Methanol extract of R. chalepensis was partitioned with hexane, chloroform, ethyl acetate, butanol and water fraction, successively. In this result, the highest activity was shown in chloroform fraction against S. zeamais. Biologically active compound derived from chloroform fraction of R. chalepensis extract was purified by using SiO2 column chromatography and prep-HPLC. The insecticidal constituent of R. chalepensis was identified as quinoline-4-carboxaldehyde by various chromatography and spectroscopic analysis methods. At 2.5 mg/disk, the most toxic activity against S. zeamais was exerted by the direct contact method (100%), followed by the fumigant method (23%). These results revealed that the contact toxicity showed 4.35 times greater than the respiration toxicity. Furthermore, these results indicate that quinoline- 4-carboxaldehyde could be useful as a new preventive agent against damage caused by stored-product insects.
Hepatocyte-specific Prominin-1 protects against liver injury-induced fibrosis by stabilizing SMAD7
Lee Hyun,Yu Dong-Min,Bahn Myeong-Suk,Kwon Young-Jae,Um Min Jee,Yoon Seo Yeon,Kim Ki-Tae,Lee Myoung-Woo,Jo Sung-Je,Lee Sungsoo,Koo Seung-Hoi,Jung Ki Hoon,Lee Jae-Seon,Ko Young-Gyu 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Prominin-1 (PROM1), also known as CD133, is expressed in hepatic progenitor cells (HPCs) and cholangiocytes of the fibrotic liver. In this study, we show that PROM1 is upregulated in the plasma membrane of fibrotic hepatocytes. Hepatocellular expression of PROM1 was also demonstrated in mice (Prom1CreER; R26TdTom) in which cells expressed TdTom under control of the Prom1 promoter. To understand the role of hepatocellular PROM1 in liver fibrosis, global and liver-specific Prom1-deficient mice were analyzed after bile duct ligation (BDL). BDL-induced liver fibrosis was aggravated with increased phosphorylation of SMAD2/3 and decreased levels of SMAD7 by global or liver-specific Prom1 deficiency but not by cholangiocyte-specific Prom1 deficiency. Indeed, PROM1 prevented SMURF2-induced SMAD7 ubiquitination and degradation by interfering with the molecular association of SMAD7 with SMURF2. We also demonstrated that hepatocyte-specific overexpression of SMAD7 ameliorated BDL-induced liver fibrosis in liver-specific Prom1-deficient mice. Thus, we conclude that PROM1 is necessary for the negative regulation of TGFβ signaling during liver fibrosis.
Suppression Effect of Purpurin Derivatives on Nitric Oxide Synthase
Lee, Hoi-Seon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
The inhibitory effects of Rubia tinctorum root-derived materials against nitric oxide (NO) production were assessed by evaluation of NO production and inducible NO synthase (iNOS) expression, and compared to those of trihydroxyanthraquinone derivatives. The inhibitory effect of 1,2,4-trihydroxyanthraquinone (purpurin) from R. tinctorum roots against NO production was 60.7 and 38.5% at 2.5 and $1.25{\mu}g/mL$, respectively. Inhibitory effect of 1,2,7-trihydroxyanthraquinone was 52.5% at $2.5{\mu}g/mL$. Suppression effects of 1,2,4-trihydroxyanthraquinone on iNOS expression were confirmed by western blot analysis.