Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

Ming Zhang,Xing Fan,Bing Fang,Chengzhen Zhu,Jun Zhu,Fazheng Ren 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 1010 CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

Performance Analysis of Packet Transport Network Communication for Integrated Wide-Area Protection

Sheng-ming Ge,Z Q Bo,Lin Wang,Zhan-feng Fan,Xing Liu,Feng-quan Zhou 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3

As a new protection, wide-area protection enables protective relaying, and automatic control based on electric power system network communications and comprehensive judgment of multi-point information, which plays an increasingly important role in the secure and stable operation of electric power system. Interaction of wide-area information relies on communication network featuring high reliability and low time delay. On the other hand, most service of power transformation station is gradually towards IP and data oriented, along with the development of smart grid. This paper aims to introduce the wide-area protection technology supported by Packet Transport Network (PTN) communication technology, with analyzing the QoS (Quality of Service) network assurance architecture of PTN network, which establishes three planes, including transport plane, management plane, and control plane, based on ASON (Automatically Switched Optical Network) technology. After demonstrating the QoS assurance system of PTN from traffic control and transmission route, this paper introduces PTN networking test. Based on detailed parameters in the test results, the transmission performance of PTN on time delay, protection, and time synchronization of various electric power communication services are analyzed, with showing that PTN can fully meet the requirements of electric power communication.

Rapid assessment of suspension bridge deformation under concentrated live load considering main beam stiffness: An analytical method

Wen-ming Zhang,Jia-qi Chang,Xing-hang Shen,Xiao-fan Lu,Tian-cheng Liu 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.1

With the gradual implementation of long-span suspension bridges into high-speed railway operations, the main beam’s bending stiffness contribution to the live load response permanently grows. Since another critical control parameter of railway suspension bridges is the beam-end rotation angle, it should not be ignored by treating the main beam deflection as the only deformation response. To this end, the current study refines the existing method of the main cable shape and simply supported beam bending moment analogy. The bending stiffness of the main beam is considered, and the main beam’s analytical expressions of deflection and rotation angle in the whole span are obtained using the cable-beam deformation coordination relationship. Taking a railway suspension bridge as an example, the effectiveness and accuracy of the proposed analytical method are verified by the finite element method (FEM). Comparison of the results by FEM and the analytical method ignoring the main beam stiffness revealed that the bending stiffness of the main beam strongly contributed to the live load response. Under the same live load, as the main beam stiffness increases, the overall deformation of the structure decreases, and the reduction is particularly noticeable at locations with original larger deformations. When the main beam stiffness is increased to a certain extent, the stiffening effect is no longer pronounced.

Risk Factors of Unsatisfactory Robot-Assisted Pedicle Screw Placement: A Case-Control Study

Qi Zhang,Ming-Xing Fan,Xiao-Guang Han,Ya-Jun Liu,Da He,Bo Liu,Wei Tian 대한척추신경외과학회 2021 Neurospine Vol.18 No.4

Objective: To identify potential risk factors of unsatisfactory screw position during robot-assisted pedicle screw fixation. Methods: A retrospective analysis of robot-assisted pedicle screw fixation performed in Beijing Jishuitan Hospital from March 2018 to March 2019 was conducted. Research data was collected from the medical record and imaging systems. Univariate tests were performed on the potential risk factors (patient’s characteristics and surgical factors) of unsatisfactory screw position during robot-assisted pedicle screw fixation. For statistically significant variables in univariate tests, a logistic regression test was used to identify independent risk factors for unsatisfactory screw position. Results: A total of 780 pedicle screws placed in 163 robot-assisted surgeries were analyzed. The rate of perfect screw positions was 93.08%, and the unsatisfactory rate was 6.92%. In patients with severe obesity (body mass index≥30 kg/m2) (odds ratio [OR], 2.459; 95% confidence interval [CI], 1.199–5.044; p=0.014), osteoporosis (T≤-2.5) (OR, 1.857; 95% CI, 1.046–3.295; p=0.034), and the segments 3 levels away from the tracker (OR, 2.216; 95% CI, 1.119–4.387; p=0.022), robot-assisted pedicle screw placement has a higher risk of screw malposition. Conclusion: During robot-assisted pedicle screw placement for patients with severe obesity, osteoporosis, and segments 3 levels away from the tracker, vigilance should be maintained during surgery to avoid postoperative complications due to unsatisfactory screw position.

Integrated Protection Unit Design for Power Networks

Zhan-feng Fan,Sheng-ming Ge,Z Q Bo,Lin Wang,Feng-quan Zhou,Xing Liu,Guo-bing Song 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.4

This paper presents an hardware design solution for integrated protection of distribution systems (Network Protection Unit) by combining transient polarity comparison technique, which is based on the detection and processing of fault generated transient current signals. The integrated protection relays installed at each substation of a distribution network are communicated with the Network Protection Unit through specially designed Packet Transport Network (PTN) for fast and reliable transmission of transient polarity current signals. The relay detects the faulted generated super-imposed current signals. The transient polarity identification algorithm is then applied to the super-imposed signals to identify the polarity of the signal detected. The Network Protection Unit can collect all the transient polarity current signals under its protection area. Then The direction of a fault is determined by comparison of the polarity of the signals derived from all the line sections connected to the substation. The actual faulted section is identified by the Network Protection Unit through comparing the directional information from various stations. Simulation results presented in the paper demonstrate the feasibility of the scheme.

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Surface plasmon resonance-enhanced solar-driven photocatalytic performance from Ag nanoparticles-decorated Ti3+ self-doped porous black TiO2 pillars

Guo Zhou,Haiyan Meng,Yan Cao,Xuejun Kou,Shuxiang Duan,Leilei Fan,Ming Xiao,Fangzhou Zhou,Zhenzi Li,Zipeng Xing 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.64 No.-

The tiny Ag nanoparticles-uniformly decorated Ti3+ self-doped porous black TiO2 pillars (Ag-TPBTPs) are prepared, which show obvious surface plasmon resonance (SPR) and extend the photoresponse to visible light and near-infrared region (∼1500 nm). The Ag-TPBTPs exhibit excellent solar-driven photocatalytic activities by mineralizing of high-toxic 2,4-dichlorophenol (∼99%), which is three times higher than that of the pristine TiO2. The remarkable solar-driven photocatalytic performance can be ascribed to the porous pillars structure offering more surface active sites, the self-doped Ti3+ and SPR effect of Ag nanoparticles improving the utilization of solar light, and enhancing the spatial separation efficiency of photogenerated charge carriers.