Caffeine 과 Glucose 투여가 지구성 운동중 대사 및 호흡기능에 미치는 효과

박해근,전종귀,홍성표,민창기 忠南大學校體育科學硏究所 1986 體育科學硏究誌 Vol.4 No.1

Twelve male students (major in physical education; non-coffee drinkers; 6 endurance athletes; 6 non-athletes) were studied to determine the effects of caffeine, and Glucose feedings on submaximal endurance performance. Each subject performed three submaximal cycling trials at an absolute intensity of 900kpm. The trials were randomized to negate any possible order or training effects. For the caffeine trial, 2.5 mg/kg body weight of caffeine in 250ml of artificially sweetened barley tea was ingested twice one hour and 30.min prior to the ride. In the glucose trial, 0.5g/kg. body weight of glucose in 250m1 of barley tea was ingested twice one hour and 30 min prior to the ride. During the control trial, 250ml of artificially sweetened barley tea was ingested twice one hour and 30 min prior to the ride. obtained data from the three trials were analyzed with one way ANOVA for repeated measures design. Significant differences between means were located using a multivariate comparison. The level of significance was established at p<0. 05. The results were summerized as follows : Ingestion of glucose elevated blood glucose and insulin levels at rest , but no significant differences in blood glucose concentration were found between the three trials . Glucose ingestion resulted in rapid hypoglycemia and inhibition of FFA mobilization during exercise. Caffeine ingestion increased significantly in VE, Vo2 and plasma osmolarity during the exercise. But caffeine ingestion failed to elevate plasma FFA concentration, HR and blood pressure significantly. Glucose or caffeine ingestion did not change in blood lactate, Hb , plasma Na+ concentration, Hit and RQ during exercise. In the above results, It was suggested that ingestion of caffeine prior to the exercise fail to increase in lipid metabolism during exorcise and may have some detrimental effects on endurance performance besides psychological ergogenic effect of caffeine, and that glucose ingestion prior to the exercise elevate blood glucose and insulin level at rest but may have detrimental effect of endurance performance because of rapid hypoglycemia caused by hyperinsulinemia.

The Korean Association of lnternal Medicine : Poster Session ; PS 1357 : Nephrology ; Age is the Strongest Effector for the Relationship between Estimated Glomerular filtration Rate and Coronary Artery Calcifi cation in Apparently Healthy Korean Adults

( Hyun Beom Chae ),( Eun Jung Rhee ),( Hae Jung Park ),( Min Kyung Lee ),( Won Seon Jeon ),( Se Eun Park ),( Cheol Young Park ),( Won Young Lee ),( Ki Won Oh ),( Sung Woo Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Cardiovascular disease (CVD) is a major clinical problem and the most common cause of morbidity and mortality in patients with chronic kidney disease (CKD). Coronary artery calcifi cation (CAC) is a potential mechanism that explains the association between renal function and cardiovascular mortality. We aimed to evaluate the association between renal function and CAC in apparently healthy Korean subjects. Methods: In 23,617 participants in the health screening program in Kangbuk Samsung Hospital health screening program from January 2010 to December 2011, estimated glomerular fi ltration rate (eGFR) was assessed by Cockcroft-Gault equation. Coronary artery calcium score (CACS) was measured by multi-detector computed tomography (MDCT). Subjects were divided into 3 groups according to the CKD Staging system with eGFR grade; stage 1: eGFR = 90 mL/min/1.73 m2, stage 2: eGFR 60-89 mL/ min/1.73 m2, stage 3: eGFR 30-59 mL/min/1.73 m2. Results: Mean age of the participants was 41.4 years, and mean eGFR was 103.6±21.7 mL/min/1.73 m2. Hypertension and diabetes were noted in 43.7% and 5.5% of the participants, respectively. eGFR showed weakly negative but signifi cant association with CACS in bivariate correlation analysis (r=-0.076, p<0.01). Mean CACS signifi cantly increased from CKD stage 1 to 3. The proportion of subjects who had CAC signifi cantly increased from CKD stage 1 to 3. Although odds ratio for CAC signifi cantly increased from stage 1 to 3 after adjustment for confounding factors, this signifi cance was inversed when age was included in the model. Age was the strongest factor that affects CAC. Conclusions: This study performed in young Korean subjects with relatively normal renal function, age was the mostly strongest effector on the association between eGFR and the degree of CAC.

Streptococcus thermophilus SKD 1006의 β-Galactosidase 유전자 구조

민해기,강국희,성문희 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

The results can be concluded that the pSK 001 contains a gene for β-gal from Str. thermophilus SKD 1006, and expressed in E. coli JM 109. A 4.2 Kb of Hpa II-Hpa II fragment(lac Z of SKD 1006) of pSK 001 was subcloned into the corresponding site of pUC 19, and named pSK 002. A 1.2 Kb of Bgl II -Bgl II fragment of pSK 002 was ligated into the corresponding site of pUC 19, and named pSK 003. The nucleotide sequences of two portions(222bp close to 5' , 143 by close to 3' end) of Bgl II -Bgl II fragment of pSK 003 were determined by Sanger, Chen and Seeburg methods. The nucleotide sequences are identical to those of the gene for β-gal of Str. thermophilus A 054.

Bifidobacterium longum KCTC 3215의 β-Galactosidase 유전자의 Cloning 및 대장균에의 발현

민해기,강국희,성문희 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

The β-D-galactosidase(β-gal) gene from Bifidobacterium longum KCTC 3215 was cloned to isolate and characterize it for potential use as a selection marker in food-grade cloning vector. Chromosomal DNA from Bif. longum KCTC 3215 was cleaved with the restriction enzyme Sau3AI and ligated to pBR 322 for transformation into E. coli JM109. A β-galactosidase positive clone was detected by its blue color on a medium supplemented with 5-bromo-4-chloro-3-indolyl-β-D-galactoside. This transformant possessed a single plasmid, designated pSK 100, which contained, in addition to the vector DNA, a 5.2 Kilobase(Kb) Sau3A1 insertion fragment coding for β-gal activity. An extract from JM 109(pSK100) contained a β-gal protein with the same electrophoretic mobility as the β-gal from Bif. longum KCTC 3215. A restriction enzyme map of pSK 100 was consisted of PvuII, HincII, PstI,AccI, EcoRI, etc.

출혈성 소화성 궤양의 장기 재발률

심기남,정훈용,양석균,홍원선,박의련,박무인,김해련,민영일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Background/Aims: Bleeding from a peptic ulcer is one of the common and serious complications associated with the rate of reported mortality, which ranges from 5% to 10%. Endoscopic therapy is effective in controlling active bleeding and reducing the emer-gency surgery, the immediate mortality rate and the incidence of early rebleeding. But few recent studies have documented the long-term recurrent bleeding rate after discharge in patients with bleeding peptic ulcers. The aim of this study was to determine the long-term recurrent bleeding rate and factors predisposing to recurrent bleeding. Methods: Eighty-eight patients with bleeding peptic ulcers discharged after medical treatment between Dec. 1990 and Jul. 1992 were included in this study and retrospectively followed up with medical records and telephone interviews. The end point of follow-up was recur-rent hemorrhage, surgery for treatment of ulcer complication, or death. Results: By July 1997, retrospective follow-up was available in 76 patients. Recurrent bleeding occurred in 23 patients (30.3%) with bleeding peptic ulcers and the median follow-up period was 69 months (range, 1 ∼79 months). The estimated cumulative recurrent bleeding rate after 1, 2, 3, 4, 5 and 6 years was 11.8%, 14.5%, 19.9%, 24.2%, 27.2% and 34.2%, respectively. There was no difference between the recurrent bleeding group and the non-recurrent bleed-ing group according to age, sex, prior NSAIDs use, previous history of bleeding or pepticulcer, site of ulcer, stigmata of recent hemorrhage at initial examination, method of treatment and amount of transfusion. Conclusion: Recurrent bleeding occurred in one-third of patients with bleeding peptic ulcers after 6 years of follow-up and one-third of recurrent bleeders rebled within 1 year. The factors predisposing to recurrent bleeding in the long-term follow-up could not be found. Therefore, further studies designed to identify factors predisposing to recurrent bleeding are needed and the evaluation of Helicobacter pylori status in bleeding pepic ulcer is needed because Helicobacter pylori is an important factor of peptic ulcer recurrence.

피부에 발생한 전이성 선암 2예

최혜영,명기범,최유원,강민정 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.7

A 73-year-old male was presented with a walnut-sized erosive plaque on the lower lip and several skin colored nodules on the neck, the left shoulder and the arm. The other case was a 74-year-old male who had been diagnosed as metastatic lung cancer of unknown origin was consulted for several ulcerative nodules on the face and the left lateral neck. Both patients were finally diagnosed as metastatic adenocarcinoma each from the lung and stomach by histopathologic finding and immunohistochemical studies.

염산 Bleomycin 병변내 주입을 이용한 사마귀 치료

강민정,임연순,최혜영,명기범 梨花女子大學校 醫科大學 醫科學硏究所 1999 EMJ (Ewha medical journal) Vol.22 No.2

연구목적 : 지금까지 난치성을 보이는 사마귀의 치료로 Bleomycin 행변내 주사의 효과가 많은 연구에서 보고된 바 있다. 이에 저자들은 본 치료방법의 효과를 사마귀의 크기, 숫자, 위치 및 유병기간에 따라 판정하여 보다 효과적인 적응 대상을 알아보고자 했다. 방법 : 1995년 3월부터 1998년 2월까지 이화여자대학교 목동병원 피부과에 비성기성 사마귀로 내원한 환자중 다른 치료에 반응하지 않았거나, 재발한 경우, 크기가 0.5㎝ 이상이거나 7개 이상의 다발성 병변 , 혹은 조감 주위 병변을 보인 133명의 환자를 대상으로 Bleomycin 병변내 주사로 치료하였다. 1회 치료후 효과와 치료 종결후 완치율을 병변은 크기, 숫자, 위치, 유병기간에 따라 비교하였다. 결과 : 1) 환자의 연령 분포는 4세에서 45세였고, 남자 59명 여자 74명으로 1:1.24 비율이었다. 2) 첫 주사 치료후 96명의 환자에서 50% 이상의 병변의 수나 크기의 감소를 보였다. 크기별로는 0.5~0.9㎝의 87.5%에서, 병소의 수별로는 단일 병소의 76.9%에서 50% 이상의 병변의 크기나 수의 감소를 보여 가장 좋은 효과를 보였으나 통계적 유의성은 없었다. 또한 조갑주의 병변은 79.1%에서, 수부는 75.8%, 족부는 72.9%에서 50% 이상의 호전을 보였으나 족서부 병변은 53.4%로 가장 낮은 낮은 효과를 보였다. 유행병 기간은 6개월 이하인 31명(86.2%)에서 50% 이상의 효과를 보여 유병기간이 짧을수록 치료효과가 유의하게 증가하였다(p<0.05) 3) 치료 종결시 3개월 이후까지 재발없이 완치된 경우는 총 93명으로 69.9%였고 치료받은 병변의 수가 증가할 수록 완치율이 감소하였다, 부위별로는 조감주위가 77.1%로 가장 높았으며, 병변의 크기에 따른 일괄된 완치율의 차이는 없었다. 병변의 기간에 따른 완치율은 6개월 이하에서 80.6%로 가장 높았으며, 유병기간이 길수록 완치율은 감소하는 양상을 보였다. 4) 부작용은 환자 중 18.4%인 24명의 환자에서 발생하였으며, 유형별로는 3일 이상 지속된 동통과 홍반, 색소 침착 및 감염이 관찰되었으나, 모두 이후 호전되었으며, 1명에서 경한 조갑 능선을 동반한 변형이 관찰되었다. 결론 : 본 연구에서 사마귀에 대한 Bleomycin 병변내 주사효과가 병변의 크기에 따라 뚜렷한 차이를 보이지 않았으며, 심한 부작용이나 전신적인 독작용없이 조감주위병변 및 급성기 병변에서 높은 완치율을 보여 좋은 적응증이 됨을 확인하였다. 아울러 Bleomycin 병변내 주사의 효과 및 안정성에 있어서 시술자가 적당한 양의 약물을 주변부위로 부터 과도한 침습없이 정확히 병변의 기저부에 도달시키는 기술이 또하나의 중요한 변수가 됨을 반영하는 결과라 사료된다. Purpose:This study was to designed to evaluate the efficacy of intralesional injection of bleomycin for common warts and plantar warts according to size, number, location, and duration and to determine the appropriate indication of this treatment for warts. Materials & Methods : The objective patients had warts being refractory to previous treatment or large than 0.5cm in size or 7 or more lesions or periungal lesions. The intralesional injection with bleomycin hydrochloride 1㎎/㎖ had been performed single to six times at 3 week interval until complete resolution. Age of the patients ranged from 4 to 45 years, and male to female ratio was 1 : 1.25(59 : 74). Results : After first treatment of bleomycin intralesional injection, a reduction of 50% or more of the primary wart area was observed in 96(72.2%) of 133 patients. 93(69.9%) of 133 treated patients experienced complete cure after 1 to 7 injections. The efficacy of intralesional bleomycin injection for warts was not closely associated with the size of warts. However the duration and number of warts was inversely correlated with the efficacy of treatment. The highest cure rate was shown in patients with periungal warts(77.1%), and lowest in patients with plantar warts(53.3%). The side effects were observed in 18.4%(24 of 133) including persistent pain(17.3%), pigmentation(3.0%), erythema(1.5%), and mild nail dystrophy(0.8%), but no evidence of systemic toxicity was found. Conclusions : The efficacy of intralesional injection of bleomycin was not significantly related to the size, but closely related to the duration, number and site of the lesion. These results partly suggest that there is some important factors affecting the efficacy of treatment of bleomycin such as duration of the lesion and skillful injection of the agent into the wart tissue.

학교체육과 지역사회체육의 연계실태와 실천방안

강전민,가경환,권창기,윤해철,손수범,변해심,신민철 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was formed the basis of propelling for being closely connecting between School PE(Physical Education) and Local Society Exercise for regarding the cooperation possibility depending on the actual situation. From this, it is helpful for evaluating the total understanding of the present condition form School PE and Local Society Exercise and grasping the realities concretely. To gain the above result, the subject of study is on 48 agencies among 50 agencies that include 30 elementary and middle schools, 15 government offices, 5 YMCA offices. After investigating the plan of providing physical activity program except result PE class and the connecting program with School PE and its activation, it comes to the conclusion below this. There is the period of possible time for propelling the cooperating model at School and Local Society PE which is special activity class after school, club activity class, discretional activity class and the vacation, weekends. And also there are many participating agencies that are school, local society, public sports center and community, the youth group, local autonomous entity PE post, prfessional physical association and community, city and countries Education Office and the sports-lover's group etc. So far, the connecting program is operated at 24 agencies which are 3 elementary school(30%), 5 middle school(50%), 4 high school(40%)m 7 government office(70%), 5 YMCA office(80%). As the reason of non-operating it, the first is the lack of the charger, spare time and considering teacher's advantage when time is arranged at the teacher and leader's part. The second is the falling behind facility and the lack of place and appliance for practicing. The third is the insufficient of connecting program at program part and increasingly number of students who are individual participating at the side of local society physical agency at school. The forth is the lack of finances for running at economy part and overpaying of using fee. The fifth is the lack of the activity time at the elementary school and putting first in importance the exam for university enterance at middle and high school. The sixth is the parents and principals and school committee have little understanding of the physical educating in common at recognition part. The seventh is the cooperating company's preparation is not enough and incomplete relation each other. Looking into the opinion for activating plan of connecting program which is classified into common contents, the result is presented the same reaction both of them that the professional leader arrangement is needed at teacher and leader's part. At the facility expansion and open program part is required for a various program. In the case of school at economy condition part, more cheaper than expenses for facilities is needed and also the renovation of the education system And at the recognition part, the positive attitude of school is needed. At the safety part, the solution of safety problem for responsibility is needed. At the administration part, the support for professional agency and the change of systemic role for connecting agency.

전화상담이 치매노인 간호제공자의 소진경험, 우울, 삶의 질, 지각된 건강상태에 미치는 영향

이해정,김기련,서지민 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: The purpose of this study is to identify the effects of telephonic counseling on burnout, depression, life satisfaction, and perceived physical health among family caregivers of older adults with dementia. Method: Subjects were randomly assigned into telephonic counseling group (n=21) and the comparison group (n=32). A weekly telephone counseling was conducted by research assistants for 12 weeks. T-test were used to answer the research questions. Result: 1) There were no significant differences between the two groups on the level of burnout, depression, life satisfaction, and perceived physical health after telephone counseling. 2) Spouse caregivers under the telephone counseling tended to report higher perceived physical health than comparison group at the post-test (t=-1.88, p=.08). Spouse caregivers under the telephone counseling tended to report higher emotional exhaustion and lower feeling of self achievement. 3) Daughter-in-law caregivers under telephone counseling showed increased feeling of self achievement, improved physical health condition, and decreased depression. Conclusion : This study showed potential effects of the problem-solving telephone counseling to improve perceived physical health and to reduce the level of burnout and depression. The findings suggest the necessity of screening most vulnerable subgroups of caregivers to increase the effectiveness of nursing intervention such as telephone counseling.

Bifidobacterium angulatum으로부터 α-galactosidase의 정제 및 특성

강대중,민해기,강국희 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

α-galactosidase는 (α-D-galactoside galactohydrolase: EC 3.2.1.22)는 다당류나 올리고당인 melibiose, raffinose, stachyose와 guar gum 등의 α-galactosidic 결합을 하고 있는 당을 분해하는 효소이다. 장내세균중 우익균이며 우세균주인 Bifidobacteria로부터 α-galactosidase의 효소학적 특성을 알아보기 위하여 배양여액으로부터 ammonium sulfate분획, 핵산의 제거, Sephadex G-200 gel filtration 및 DEAE-Sephadex A-50 ion exchange chromatography 등의 4단계 정제과정을 거쳐 정제한 결과 약 8배로 정제되어 단일 단백질로 분리하였다. 또한, 효소분해산물을 알아보기 위하여 TLC에 의하여 당분해 및 올리고당의 생성을 알아보았다. 정제효소의 활성 최적온도는 37℃, 최적 pH는 7.0이었다. 효소활성이 K^+, Zn^2+과 같은 금속이온과 8-Hydroxyquinoline, Sodium Bisulfite 등에 의해선 그다지 저해되지 않았으나, Hg^+, Cu^2+과 p-Chloromercuribenzoic acid에 의해선 저해되었다. 효소의 분자량이 301, 995, 합성기질인 PNPG에 대한 K_m은 0.069mM, V_max는 347.22μmol/min.mg protein이었다. To elucidate enzymatic properties of α-galactosidase (EC 3.2.1.22) from Bif.angulatum. α-galactosidase was purified to homogeneity by ammonium sulfate fractionation, protamine sulfate precipitation, sephadex G-200 gel filtration, and DEAE-sephadex A-50 ion exchange chromatography. The purified α-galactosidase was found tobbe homogeneous by SDSpolyacrylamide gel electrophoresis. The molecular weight of this enzyme, estimated by sepadex G-200 gel filtration, was about 301,995. The optimum conditions for the enzyme reaction was pH 6.5 to 7.0 at 37℃. The purified enzyme was stable at 45℃ or below and in buffer at pH 6 to 7.0. The activity was inhibited by mercury, copper ion, and p-chloromercuribenzoic acid. The kinetics of this enzyme, with p-nitrophenyl-α-D-galactoside as substrate, was determined : K_m was about 0.069mM and V_mas was 347.22 μmoles/min mg protein.