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서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.
DEMS(Diethoxymethylsilane) precursor를 이용한 PECVD 저유전물질 박막증착연구
강민구,김대희,김영철,서화일,Kang, Min-Goo,Kim, Dae-Hee,Kim, Yeong-Cheol,Seo, Hwa-Il 한국반도체디스플레이기술학회 2008 반도체디스플레이기술학회지 Vol.7 No.4
We studied deposition of low-k SiOCH dielectric film by PECVD. DEMS(diethoxymethlysilane) precursor, which has two ethoxy groups along with one methyl group attached to the silicon atoms, was used as precursor. The SiOCH film was deposited as a function of oxygen flow rates ranging from 0 to 100sccm. The deposition rate($\AA$/min) of SiOCH film was increased due to the increase of oxygen radical as a function of $O_2$ flow rates. The dielectric constant was decreased from 3.0 to 2.77, as the film was annealed at $450^{\circ}C$ for 30 min. So, it could account that the dielectric constant changes sensitively with $O_2$ flow rates. Also, the leakage current of the annealed film exhibited stable curve than that of asdeposited. These results were caused by the increase of Si-O-Si group and decrease of Si-CH group and OH group within the film by annealing.
Facile Synthesis and Radioiodine Labeling of Hypericin
Kim, Sang-Wook,Park, Jeong-Hoon,Yang, Seung-Dae,Hur, Min-Goo,Kim, Yu-Seok,Chai, Jong-Seo,Kim, Young-Soon,Yu, Kook-Hyun Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8
Hypericin (1,3,4,6,8,13-hexahydroxy-10,11-dimethylphenanthro[1,10,9,8-opqra]perylene-7,14-dione), an antidepressant which is also known to be a potent protein kinase C (PKC) inhibitor was synthesized as a precursor for radioiodine labeling via two step reactions. Malignant glioma cells express higher PKC activity compared to untransformed glial cell. Here we report the synthesis and radioiodine labeling of hypericin as a potential brain tumor imaging radiopharmaceutical. The reference compound, 2-iodohypericin, and its radiolabelled analogues, 2-[$^{123}I$]iodohypericin and 2-[$^{124}I$]iodohypericin have been prepared by the reaction of hypericin with NaI or [$^{123}I$]NaI or [$^{124}I$]NaI. The labeling yield was 60-65% for each analogue and the optimal reaction time was 10 min. The purification and isolation of the labelled products were achieved by a reversed-phase HPLC.
Min-Goo Seo,Oh Deog Kwon,곽동미 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
In a population-based study with 4 years of follow up, we evaluated the prevalence of Coxiella burnetii in cattle on Ulleung Island, Korea. In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3-1.0% in the period 2011-2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide se- quences of the 2 samples showed high (98.4-100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the trans- mission of C. burnetii on Ulleung Island, control strategy should include biosecurity improvement in surveillance, livestock management, administering suitable tests before purchasing animals to detect C. burnetii shedders, and restricting movements between herds.
Min-Goo Seo,In-Ouh Ouh,Seung-Hun Lee,Ui-Han Son,Paul John L. Geraldino,Man Hee Rhee,Oh-Deog Kwon,Tae-Hwan Kim,Dongmi Kwak 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.3
Anaplasmosis is a tick-borne, non-contagious, zoonotic disease caused by Anaplasma spp., which include Anaplasma marginale, A. centrale, A. phagocytophilum, A. platys, A. ovis, and A. bovis. Recently, in Korea, the prevalence of Anaplasma spp. has been investigated in some animals, such as dogs, horses, goats, cats, and Korean water deer. In cattle, A. marginale is the most virulent species and regarded as the typical type of species. However, data on the seroprevalence of Anaplasma spp. in cattle in Korea during the last decade is limited. This study was designed to investigate the seroprevalence of bovine anaplasmosis in Korea. From 2010 to 2013, blood samples were collected from 568 cattle. Forty animals (7.0%) tested seropositive for Anaplasma spp. by cELISA. Despite that current bovine anaplasmosis seropositivity rate in the Gyeongsangbuk-do is lower than those in tropical countries, anaplasmosis needs to be regarded as a concerning disease. The identification of the specific Anaplasma species infecting cattle in this province requires additional molecular studies. Moreover, further monitoring and control programs for bovine anaplasmosis is required, and the information from this study will be beneficial to develop these programs.
Seroprevalence of Coxiella burnetii infection in cattle on Ulleung Island, Korea
Seo, Min-Goo,Ouh, In-Ohk,Kim, Young-Hoan,Kim, Joong-Kew,Kwon, Oh-Deog,Kwak, Dongmi The Korean Society of Veterinary Science 2018 大韓獸醫學會誌 Vol.58 No.3
We assessed the seroprevalence of Coxiella burnetii (C. burnetii) in cattle on Ulleung Island, Korea in a population-based follow up study for 4 years and determined the spatial distribution and risk factors associated with C. burnetii. The seroprevalence of C. burnetii was determined to be 1.4-2.0% during 2011-2014. Overall, nine cattle from three farms that tested seropositive showed C. burnetii antibody seroconversions between 2011 and 2014. The number of seropositive cattle was low, suggesting that movement of and contact between animals was possible risk factors for the transmission of C. burnetii.
Seo, Goo-Young,Lee, Jeong-Min,Jang, Young-Saeng,Kang, Seung Goo,Yoon, Sung-il,Ko, Hyun-Jeong,Lee, Geun-Shik,Park, Seok-Rae,Nagler, Cathryn R.,Kim, Pyeung-Hyeun Elsevier 2017 Cellular immunology Vol.322 No.-
<P><B>Abstract</B></P> <P>The present study extends an earlier report that retinoic acid (RA) down-regulates IgE Ab synthesis in vitro. Here, we show the suppressive activity of RA on IgE production in vivo and its underlying mechanisms. We found that RA down-regulated IgE class switching recombination (CSR) mainly through RA receptor α (RARα). Additionally, RA inhibited histone acetylation of germ-line ε (GL ε) promoter, leading to suppression of IgE CSR. Consistently, serum IgE levels were substantially elevated in vitamin A-deficient (VAD) mice and this was more dramatic in VAD-lecithin:retinol acyltransferase deficient (LRAT<SUP>−/−</SUP>) mice. Further, serum mouse mast cell protease-1 (mMCP-1) level was elevated while frequency of intestinal regulatory T cells (Tregs) were diminished in VAD LRAT<SUP>−/−</SUP> mice, reflecting that deprivation of RA leads to allergic immune response. Taken together, our results reveal that RA has an IgE-repressive activity in vivo, which may ameliorate IgE-mediated allergic disease.</P> <P><B>Highlights</B></P> <P> <UL> <LI> RA represses IgE CSR through RA receptor alpha (RARα). </LI> <LI> RA inhibits histone acetylation of Ig germ-line ε (GL ε) promoter. </LI> <LI> RA has an IgE-repressive activity in vivo, which may ameliorate IgE-mediated allergic disease. </LI> </UL> </P>
Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column
Min-Goo Seo,Tae-Hwan Kim,Oh-Deog Kwon,Kyu-Shik Jeong,Young Ju Lee,Seung-Joon Kim,Sang-Joon Park,Young Sam Kwon,Dong-Mi Kwak 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.2
Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
Seo, Min-Goo,Ouh, In-Ohk,Lee, Haeseung,Geraldino, Paul John L.,Rhee, Man Hee,Kwon, Oh-Deog,Kwak, Dongmi Elsevier 2018 Veterinary microbiology Vol.226 No.-
<P><B>Abstract</B></P> <P>Bovine anaplasmosis is a tick-borne, infectious, non-contagious disease caused by <I>Anaplasma marginale</I>, <I>A. centrale</I>, <I>A. bovis</I>, and zoonotic <I>A. phagocytophilum</I>. Recently, <I>Anaplasma capra</I> detected in goats was identified as a novel zoonotic pathogen. To determine whether <I>A. capra</I> can infect bovines, we used PCR to differentially diagnose <I>Anaplasma</I> spp. in 1219 South Korean cattle by performing multilocus gene typing and restriction fragment length polymorphism (RFLP). Nucleotide sequencing and phylogenetic analysis detected the 16S rRNA gene of <I>A. bovis</I> and four genes from <I>A. capra</I> in 12 (1.0%) and five (0.4%) cattle, respectively. Supplementary discrimination between <I>A. bovis</I> and <I>A. capra</I> was accomplished by RFLP. The 16S rRNA, <I>msp4</I>, <I>groEL</I>, and <I>gltA</I> genes of <I>A. capra</I> identified in this study had much lower degrees of identity to those in <I>A. centrale</I> and other <I>Anaplasma</I> spp. <I>A. phagocytophilum</I> was not detected in any of the tested cattle. Although the prevalence was low, this study suggests the potential of cattle to serve as reservoirs of <I>A. capra</I>. Thus, further studies are needed to clarify the pathogenesis of <I>A. capra</I> in cattle and its possible involvement in transmission to humans.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Differential identification of <I>Anaplasma</I> spp. in 1219 South Korean cattle by PCR and RFLP. </LI> <LI> <I>Anaplasma bovis</I> and <I>A. capra</I> were detected in 1.0% and 0.4% using 16S rRNA and multilocus genes. </LI> <LI> This study suggests the potential of cattle to serve as reservoirs of <I>A. capra.</I> </LI> <LI> The occurrence of a new emerging <I>A. capra</I> strain with public health and veterinary significance in Korean cattle. </LI> </UL> </P>