경북 동부지역 번식장애 소에서의 큐열 항체 보유율

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),김상윤 ( Sang Yun Kim ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011∼2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.

경북지역 집합유와 젖소에서 큐열 항체 보유율 조사

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),김인경 ( In Kyoung Kim ),조민희 ( Min Hee Cho ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

Q fever is a rickettsial infection caused by Coxiella burnetii that is transmitted from animals to humans. Modes of transmission to humans include inhalation, tick bites and ingestion of unpasteurized milk or dairy products. This survey was aimed at monitoring the seroprevalence of C. burnetii in bulk-tank milk (BTM) in Gyeongbuk province. In addition, the seroprevalence of C. burnetii was investigated at the herd level of dairy cattle in eastern Gyeongbuk province in which many dairy cattle are reared. Among 324 BTM samples collected from 20 country areas, 175 (54%) BTM samples from 15 (75%) country areas were positive for C. burnetii by ELISA. By regions, the seroprevalence of BTM samples in eastern, central, western and northern areas of Gyeongbuk province were 62.7%, 48.4%, 45.1%, and 41.4%, respectively. When analyzed in the dairy cattle reared in the eastern area where high seroprevalence occurred in BTM samples, 119 (24.2%) out of 492 dairy cattle were positive for C. burnetii. Seroprevalence of C. burnetii in dairy cattle was increased with daily milk yield of farm (P<0.05) and age (P<0.001). Since seroprevalence of C. burnetii is relatively high in both BTM samples and dairy cattle reared in Gyeongbuk province, further studies on the high risk farms and herds are needed to evaluate infection status and appropriate control programs in this region.

Molecular detection of <i>Anaplasma phagocytophilum</i>-like <i>Anaplasma</i> spp. and pathogenic <i>A. Phagocytophilum</i> in cattle from South Korea

Seo, Min-Goo,Ouh, In-Ohk,Kwon, Oh-Deog,Kwak, Dongmi Elsevier 2018 Molecular phylogenetics and evolution Vol.126 No.-

<P><B>Abstract</B></P> <P> <I>Anaplasma phagocytophilum</I> is the causative agent of human granulocytic anaplasmosis and tick-borne fever in domestic ruminants. Differential diagnosis of zoonotic and pathogenic tick-borne diseases like granulocytic anaplasmosis is important for the efficient implementation of control programs. Thus, the differentiation of pathogenic <I>A. phagocytophilum</I> from non-pathogenic <I>A. phagocytophilum</I>-like (APL) <I>Anaplasma</I> spp. is essential. Recent molecular analyses of APL revealed its distinct phylogenetic position from <I>A. phagocytophilum</I>. This study was conducted to detect <I>A. phagocytophilum</I> and genetically related strains in 764 cattle in South Korea using PCR and restriction fragment length polymorphism assays. APL clade A and <I>A. phagocytophilum</I> were identified in 20 (2.6%) and 16 (2.1%) cattle, respectively, with 16 cattle (2.1%) displaying co-infection. The 16S rRNA sequences of APL clade A were similar (98.3–99.9%) to those clustered in the APL clade A from eastern Asia. The <I>A. phagocytophilum</I> 16S rRNA sequence shared 98.6–100% identity to those of the <I>A. phagocytophilum</I> group. We used PCR to amplify the <I>groEL</I> and <I>msp2</I> genes from the 20 samples positive for the 16S rRNA gene and found that 16 were positive for the <I>groEL</I> sequences in the APL clade A, which showed identity (82.8–84.4%) to those clustered in the APL clade A from Japan. Amplification of <I>msp2</I> was unsuccessful. The co-infection results suggested sequence diversity in <I>Anaplasma</I> spp. Till date, both <I>A. phagocytophilum</I> and APL have been reported to be distributed separately in several animals throughout South Korea. This report is the first co-detection of <I>A. phagocytophilum</I> and APL in Korean cattle using molecular methods. Further studies are needed to provide additional molecular background and trace the evolutionary tree of <I>Anaplasma</I> species in animals and ticks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genotyping of <I>A. phagocytophilum</I> (AP) and <I>A. phagocytophilum</I>-like (APL) strains. </LI> <LI> Sixteen AP and 20 APL were detected in 764 cattle by 16S rRNA sequencing and RFLP assay. </LI> <LI> The APL strains were more divergent in the <I>groEL</I> gene than in the 16S rRNA gene. </LI> <LI> Amplification of the <I>groEL</I> and <I>msp2</I> gene was unsuccessful in AP. </LI> <LI> First detection of AP and APL in Korean cattle and possible circulation of new APL. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

울릉도 소의 전염성 병원체 감염률 조사

서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4

Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.

Focal cerebral ischemic injury decreases calbindin expression in brain tissue and HT22 cells

In-Ohk Ouh,Young-Min Kim,Phil-Ok Koh 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

Calbindin is a calcium binding protein that controls intracellular calcium levels and has a neuroprotective function against apoptotic stimuli. We investigated the expression of calbindin in ischemic brain injury. Focal cerebral ischemia was induced in male rats by middle cerebral artery occlusion (MCAO) and cerebral cortices were collected 24 h after MCAO. Cerebral ischemia significantly increased infarct volume. RT-PCR and Western blot analyses showed that MCAO injury induced a decrease of calbindin expression. Moreover, immunohistochemical staining showed that the number of calbindin-positive cells decreased in ischemic regions of MCAO-operated animals. In cultured hippocampal-derived cell lines, glutamate exposure increased intracellular Ca<SUP>2+</SUP> concentrations and decreased calbindin expression. Taken together, both in vivo and in vitro results demonstrated decreases of calbindin after neuronal cell injury. These results suggest that decreases of calbindin in ischemic brain injury contribute to neuronal cell death.

Antibody responses after vaccination against equine influenza virus in the Republic of Korea in 2019

In-Ohk Ouh,Min Ji Kim,Hyeonhae Choi,Hyoung seok Yang,Sang Kyu Lee,Seo Young Moon,Suyeong Yun,Bang-hun Hyun,Soo dong Cho,Choi-Kyu Park,Yoon-Hee Lee 한국예방수의학회 2020 예방수의학회지 Vol.44 No.3

In this study, antibody responses after vaccination against equine influenza were investigated among 1,591 horses in the Republic of Korea using the hemagglutination inhibition (HI) test. Equine influenza has not occurred since 2011 and a commercial vaccine against H3N8 has been used. The equine influenza virus, A/equine/South Africa/4/03 (H3N8), was used as the antigen in the HI assay. The mean seropositive rate was 90.5% in 2019. Except for stallion whose seropositive rate was 78.5%, all seropositive rates of other horse types were over 90%. Regionally, except for Gangwon-do and Jeju-do whose seropositive rates were 89.0% and 87.1%, all seropositive rates in other provinces were over 90%. In the future, more through vaccination against equine influenza needs to be done based on this investigation result.

Seroprevalence of the swine influenza A virus in domestic pigs in the Republic of Korea

In-Ohk Ouh,Seo Young Moon,Mi-Ja Park,Min Ji Kim,Min-Ho Seong,Jong-Sik Bak,Hyoung seok Yang,Hyeonhae Choi,Suyeong Yun,Bang-hun Hyun,Soo dong Cho,Yoon-Hee Lee,Choi-Kyu Park 한국예방수의학회 2020 예방수의학회지 Vol.44 No.3

Influenza A virus (IAV) causes respiratory disease in birds and mammals, including pigs and humans. Infection by IAV in pigs increases not only economic losses in the swine industry but also the emergence of novel IAV variants via gene reassortment, which is important due to the susceptibility of both birds and humans to IAV. This study provides serological data obtained during a study to detect IAV infections in pigs in the Republic of Korea during 2018 and 2019. A total of 1,559 samples were collected from 74 domestic pig farms. Hemagglutination inhibition (HI) assays were performed using the A/Swine/Korea/25-13(H1N1), A/Swine/Korea/E102 (H1N2), and A/Swine/Korea/Cy10/2007 (H3N2) viruses as antigens. The HI assay results showed that 266 of the 1,559 samples were seropositive (17.0%). Among these, H1N1, H1N2, and H3N2 comprised 7.3% (114), 6.0% (93), and 8.8% (137) of the 1,559 samples, respectively. Co-infections of H1N1/H1N2, H1N1/H3N2, H1N2/H3N2 and H1N1/H1N2/H3N2 were observed in 2.1% (31), 1.5% (23), 1.5% (24), and 0.8% (13) of the 1,559 samples, respectively. Interestingly, IAV infections were detected in all nine provinces of the country.

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

OUH, In-Ohk,SEO, Min-Goo,SHAH, Fawad-Ali,GIM, Sang-Ah,KOH, Phil-Ok The Japanese Society of Veterinary Science 2014 The Journal of veterinary medical science Vol.76 No.3

<P><B>ABSTRACT</B></P><P>Testicular torsion is a urological emergency that leads to serious testicular damage and male infertility. We performed this study to identify specific proteins that are differentially expressed in response to testicular torsion and detorsion-induced ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating the left testis 720° in a clockwise direction for 1 hr, and then, detorsion was performed for 24 hr. After this testicular tissues were collected, protein analysis was performed using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury resulted in serious histopathologic damage to the germinal cells in the seminiferous tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific protein spots with a greater than 2.5-fold change in intensity between the sham-operated and testicular I-R groups were identified by mass spectrometry. Among these proteins, levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins, ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were decreased in the testicular I-R group compared to the sham-operated group. Moreover, Western blot analysis clearly showed the decrease of these proteins in the testicular I-R group. These proteins have spermatogenesis and anti-oxidative functions. These findings suggest that testicular I-R results in cell death due to altered expression of several proteins with spermatogenesis and anti-oxidation functions.</P>

Surveillance of canine influenza A virus in the Republic of Korea from 2016 to 2021

Seong-In Lim,Min Ji Kim,In-Ohk Ouh,Min-Ji Kim,MiJung Kwon,Gyeong Gook Park,Eui Hyeon Lim,Eun-Ju Kim,Yeun-Kyung Shin,Soo Dong Cho,Bang-hun Hyun,Yoon-Hee Lee 한국예방수의학회 2022 예방수의학회지 Vol.46 No.4

Influenza A viruses (IAVs) are members of the family Orthomyxoviridae and genus Orthomyxovirus. Avian and mammalian species are the host of IAVs, which includes humans and dogs. Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory diseases in dogs. This study monitored the antigen and antibody against CIV in dogs in the Republic of Korea (ROK) from 2016 to 2021. One thousand and seventy-two nasal swabs and 1,545 blood samples were collected from animal hospitals and animal shelters. Five nasal swabs in 2017 and seven in 2018 from stray dogs were positive for CIV according to RT-PCR. The prevalence of H3N2 CIV ranged from 9.5% to 24.8%, according to the hemagglutination inhibition (HI) assay. On the other hand, none of the serum samples from 2018 to 2021 showed seropositivity against the avian H5, H7, and H9 viruses. The HI titers for H3N2 ranged from 16 to 512. The distribution of HI titer 16–32 was 57.6% in seropositive samples. The pet dogs were vaccinated against CIV, but the stray and military dogs were unvaccinated. In 2017 and 2018, the seroprevalence of CIV in stray dogs was higher than in the other years, and viral RNA was detected in nasal swabs. It may mean previous exposure of stray dogs to CIV. With the increasing number of pet dogs and the close contact between humans and dogs, canines could serve as an intermediate host for transmitting IAVs to humans. Therefore, continuous surveillance of CIV is needed for public health and the potential emergence of novel zoonotic viruses.

경북 동부지역 꿀벌에서 주요 병원체의 분자생물학적 검출

우인옥 ( In Ohk Ouh ),도재철 ( Jae Cheul Do ),서민구 ( Min Goo Seo ),정태남 ( Tae Nam Jeong ),조민희 ( Min Hee Cho ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.1

The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010∼2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.