Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

중앙아시아 벽화 보존처리(1) : 벽화(본4074, 본4096)의 상태조사

강형태,이용희,유혜선,김연미,조연태,시고 아오키,노리코 야마모토,켄타로 오바야시 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

국립중앙박물관은 용산 새 박물관의 전시 유물로 선정된 중앙아시아 벽화의 연차적인 보존처리를 위해 “독립행정법인 일본 도쿄문화재연구소’’와 공동연구를 모색하게 되었다. 2001년에 본4074, 본4096 작은 벽화편 2점의 보존처리를 시작하면서 벽화의 균열 박락 등 손상상태, 구조와 구성재료, 이전 보존처리에서 적용된 재료와 방법에 대한 기초조사를 실시하였다. 조사결과 본4074, 본4096 2점의 벽화는 흙과 지푸라기를 반죽하여 만든 벽체에 석고를 발라 바탕으로 하고 그 위에 채색을 한 것으로 나타났으며 벽체 속에 포함된 지푸라기의 방사선 탄소연대측정 결과 본 벽화는 10세기 말에서 13세기 초에 만들어진 것으로 나타났다. 또한 X-선회절 분석 결과 화면의 흰색 바탕은 gypsum[Ca(SO₄)·2H₂O]과 CaSO₄, Calcite(CaCO₃)가 적색계통은 연단(鉛丹:Pb₃O₄)과 led arsenate[Pb(As₂O₆)], 녹색계통은 Cuprite(Cu₂O)와 arsenolite(As₂O₃), arsenic oxide(As₂O₄) 등이 사용된 것으로 조사되었다. This article is about a joint project carried out by the National Museum of Korea and the Tokyo Cultural Properties Research Institute for the conservation of central Asia Wall painting that has been selected for the exhibition at the new Seoul National Museum of Korea at Yongsan. The investigation of the wall painting revealed very useful information. This includes the condition of the object, and the identification of evident damage, such as cracks, loss of pigment, plus materials and methods employed during the object's creation, as well as previous conservation treatment. The object was mainly made by applying plaster to the body (wall) that consisted of a mixture of soils and rice straws. Then, on the surface of the wall-painting, pigments were used to draw and to colour it. As a part of the investigation, radiocarbon dating was conducted using straw samples taken from the object. The result indicates that the object is probably dated form between the end of the 10th Century and the beginning of the 13th Century. The result of X-ray diffraction also revealed the composition of the pigments used on the surface. These are 1. gypsom[Ca(SO₄)·2H₂O], CaSO₄ and Calcite(CaCO₃) and Calcite(CaCO₃) that were used for the white background. 2. Pb₃O₄ and led Arsenate [Pb(As₂O₆) that were used for the red colouring. 3. Cuprite(Cu₂O), Arsenolite(As₂O₃) and Arsenic Oxide(As₂O₄) that were used for the green colouring.

형광 in situ hybridization 방법에 의한 c-myc 유전자의 전위 및 증폭에 관한 연구

김경채,박선화,김강련,김미영,전용혁 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

황남대총 98호분 출토 유리의 과학적 분석

조경미,유혜선,강형태 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

황남대총 98호분 북분과 남분에서 출토된 유리시료 40점에 대한 과학적인 분석을 실시하였다. 유리의 성분조성은 주사전자현미경-에너지분산형분광기(SEM-EDS)를 사용하여 정량분석 하였고 다변량해석법을 통하여 시료를 분류하였다. 그 결과 시료 40점 모두 Na₂O를 약 20%정도 함유한 소다-석회(Na₂O CaO-SiO₂)유리임을 확인하였으며, 다시 5개 주성분(SiO₂, AI₂O₃, Na₂O, CaO, K₂O)으로 다변량해석[주성분분석(PCA)]을 실시한 결과 2개의 群으로 분류되었다. I 群에 포함된 시료는 Al₂O₃의 농도가 9.7%로 높고 CaO의 농도는 2.2%인데 비하여 Ⅱ群에서는 각각 3.2%,4.9%의 범위로 나타났다. 특히 시료 No.12의 노란 색으로 편석된 부분을 미소부위 XRD로 분석한 결과 PbSnO₃임을 국내 최초로 확인할 수 있었다. 鉛을 함유한 시료 중 No.12, 17은 열이온화질량분석기(TIMS)로 납동위원소비를 측정하였고 선형판별식 분석법(SLDA)을 이용하여 납의 산지를 추정하였다. 그 결과 각각 중국 남부, 한국 남부의 납광석을 사용한 것으로 나타났다. Elemental analysis of 40 glass samples from the Northern Tomb and the Southern Tomb of Hwangnam-daechong No. 98 was performed. Fourteen compositions of each sample were analyzed quantitatively by SEM-EDS and glass samples were classified by multivariate analysis such as PCA. All of 40 samples were confirmed to be Na₂O CaO-SiO₂ system with about 20% of Na₂O. Samples were classified into two groups by doing PCA on concentrations of 5 major elements (SiO₂, AI₂O₃, Na₂O, CaO, K₂O). Samples included in group I showed the concentration of AI₂O₃ is about 9.7% and that of CaO, about 2.2%. In group II, concentration of Al₂O₃ is about 3.2% and that of CaO, about 4.9%. Especially yellow grains embedded in sample No.12 were shown to be PbSnO₃ by micro XRD, which was the first coloring material ever found in Korea. Lead isotope ratios of samples No.12 and No.17 which contained lead were measured by TIMS. The origin of lead was traced by means of multivariate analysis such as SLDA. The result showed that lead from southern China and southern Korea had been used for making glass.

마약길항제의 방출 제어형 제제 (제2보) : 나록손 이식제제의 생체적합성 및 약물속도론적 평가 Biocompatibility and Pharmacokinetics of Naloxone Implant

문미란,박주애,이승진,김형국,김길수 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

For the effective administration of narcotic antagonist, the application of sustained release implantable systems with biodegradable polyphosphazene was examined. Using poly[(diethyl glutamate)-co-(ethyl glycinate) phosphazene]. the implantable devices containing naloxone hydrochloride were prepared and in vivo implantation studies were carried out subcutaneously in rat and rabbit with this preparation for the biocompatibility and pharmacokinetics. The histological finding in rats at initial time period was the inflammation that occurred focally around the implants, but they were showed subsequent mild and limited chronic inflammations and the irreversible changes such as necrosis and degeneration of the muscle or connective tissues were not observed. Therefore the placebo and naloxone implants are considered to be biocompatible formulations histologically. In pharmacokinetic studies, the release of naloxone from the naloxone implants into blood plasma was maintained in 192 hours, but the initial burst effect was observed. If this problem was solved, the application for the narcotic antagonist sustained release systems can be expected.

취학 전 6세 여아의 신체활동이 체력에 미치는 영향

김미지,박형하,박판수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study is to provide an information about pre-school female children's physical activity effect on physical strength. Total sixty individuals participate in this study - three times measure for thirty two weeks. between experimental group, 30 infants' physical aptitude group in Busan in which pre-school female children take part in regular physical activity three times per week two hours each time and control group, 30 kindergarten children who take part in irregular physical activity. After analyzing gap between two groups and change after physical activity. the study results are as follows. Case of physique change. in standing height. after physical activity experimental group (p<.001) and control group(p<.01) each groups shows significant increase and in body weight. control group shows significant increase(p<.05) and in chest girth. experimental group shows significant increase(p<.01). Between two groups. experimental group shows more significant difference than control group(p<.05) only in chest girth. Case of physical strength change, in grip strength. after physical activity both groups shows significant increase(p<.001) and in sit-up. experimental group(p<.00l) and control group(p<.05), each group shows significant increase and in trunk flexion, experimental group shows significant increase(p<.01) and in one foot balance with eyes closed, both groups shows significant increase(p<.001) and in 10m shuttle run, experimental group shows significant increase(p<.01) and in standing broad and long jump, experimental group(p<.001) and control group(p<.01), each group shows significant increase. Between two groups, in comparison with control group experimental group shows significant difference in sit-up(p<.01), one foot balance with eyes closed(p<.001), 10m shuttle run(p<.05) and standing broad and long jump(p<.01).

광합성 세균 Rhodospirillum rubrum S1이 생성한 Catalase-Peroxidase의 부분 정제 및 특성 규명

김영미,이동헌,강형일,오덕철 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

호기적으로 배양한 광합성 세균 Rhodospirillum rubrum SI은 5 가지의 catalase를 생성함을 알 수 있었다. 그 중 peroxidase의 기능도 동시에 갖는 catalase-peroxidase(Cat-3)를 부분 정제하고 그 특성을 조사하였다. 부분정제된 catalase-peroxidase의 수율은 catalase가 1.6% 그리고 peroxidasse가 5.1% 였으며, 정제 배수가 4.6배와 14배로 증가한 효소를 얻을 수 있었는데, catalase보다 peroxidase의 정제배수가 더 높았다. pH에 대한 영향은 catalase활성은 pH6에서, peroxidase활성은 pH5에서 가장 높게 나타났다. 온도에 대한 영향은 catalase와 peroxidase 활성이 모두 30℃에서 최고의 활성을 보이는 것으로 나타났으며, 온도에 대한 효소의 안정성은 50℃에서 catalase가 peroxidase보다 더 안정함을 알 수 있었다. 부분정제된 catalase-peroxidase에 유기용매와 10mM 3-amino-1,2,4-triazole을 처리한 결과, 유기용매에 대해 catalase의 활성은 79%, peroxidase의 활성은 85%까지 억제됨을 알 수 있었으며, 10mM 3-amino-1,2,4-triazole에 대한 각 효소의 활성은 그대로 유지되고 있었다. 전형적인 heme단백질 효소들의 저해제로 알려진 NaCN, NaN3, NH20H를 농도별로 처리한 결과, NaCN인 경우 catalase는 8.72×10-s M의 농도에서, peroxidase는 5.1×10-s M의 농도에서, NaN3에서 catalase는 4.2×10-7 M, peroxidase는 3.2×10-7 M, NH20H에서는 catalase인 경우는 2.0×10-7 M, peroxidase인 경우는 2.5×10-7M 농도에서 억제되었으며, 이들 저해제들은 catalase활성과 peroxidase활성을 동시에 저해하는것으로 나타났다. Five different catalases have been already found in the aerobically grown photosynthetic bacterium Rhodospirillum rubrum S1. Among them, a bifunctional catalase-peroxidase, designated Cat-3 was partially purified and characterized. The enzyme was purified through four steps in 1.6% yield for catalatic activity and 5.1% yield for peroxidatic activity. On the basis of catalatic activity, the protein purification increased nearly 4.6-fold, whereas for peroxidatic activity, an approximately 14-fold purification was achieved. The optimum pHs of the catalatic and peroxidatic activities were 6 and 5, respectively, and the optimum temperature for both activities was 30℃. The catalatic activity of the enzyme maintained at 50℃ for lh was more stably than peroxidatic activity. The catalatic and peroxidatic activities were inhibited about 79 % and 85 % by exposure to organic solvent(ethanol/chloroform), respectively. Both enzymatic activities were not neally inhibited by lOmM 3-amino-1,2,4-triazole. Fifty percent enzyme inhibition of the catalatic activity was reached with 2.0×10^(-7)M, hydroxylamine, 4.2×10^(-7)M azide and 8.7×10^(-6)M cyanide, and that of the peroxidatic activity was obtained with 2.5×10^(-7)M hydroxylamine, 3.2×10^(-7)M azide and 5.1×10^(-6)M cyanide.

Aluminum응집제들에 의한 금속이온들의 응집에 관한 연구

김미향,최범석 慶熙大學校 材料科學技術硏究所 1999 材料科學技術硏究論集 Vol.12 No.-

Coagulation efficiencies of inorganic ions by aluminum based coagulants including alum, PAC, PACS were studied. Pb, As, Cu, Hg ions shows good coagulation efficiences with all the coagulants. Residual amount of Hg is also decreased by the precipitation as a hydroxide. The maximum coaulation efficiency is observed at the neutral pH and the efficiency is decreased with lowering pH, except Se. The coagulation efficiencis are decresed with an order of PACS, PAC, and alum. Cr, Cd shows lower coagulation efficiencies and Ni and Mn are not coagulated with all the coagulants.

시판잇솔의 규격과 품질에 관한 조사 연구

김미형 광주보건대학 1993 論文集 Vol.18 No.-

It would seem that oral hygiene is one of the high sports characteristic only of the last decade or so. Tooth brushing is the most essential method for prevention of dental caries and periodontal disease. Nowadays, many domestic and imported toothbrushes were introduced, advertised and sold to the common people . Also concerns of the people to these toothbrushes are suitable to Koreans. The samples of investigation are 63 kinds of, 77pieces toothbrushes, include in domestic and imported toothbrushes which divided in adultbrush, childbrush. The results were follows: 1. Head size;length, width and height of toothbrushes-toothhead are more larger compared with Korean's toothsize both in the adult and the child. 2. As a result of the classification of vertical and horizontal tufts, domestic-made adultbrush which 4-row-tufts were 90.3% and foreign-made one which 3-row-tufts were 61.9% and childbrushes were most of 3-row-tufts. 3. In order to improve the quality of toothbrushes and manufactures toothbrush suitable to Koreans require for consistent study.

비만세포주에서 Histamine 유리에 관한 연구

김형룡,국윤아,신민,이홍수,원진숙,김형민,이영미,정헌택,이혜숙,안녕형 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1