CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin

Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.

Operation optimization of auxiliary electric boiler system in HTR-PM nuclear power plant

Du Xingxuan,Ma Xiaolong,Liu Junfeng,Wu Shifa,Wang Pengfei 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.8

Electric boilers (EBs) are the backup steam source for the auxiliary steam system of high-temperature gas-cooled reactor nuclear power plants. When the plant is in normal operations, the EB is always in hot standby status. However, the current hot standby operation strategy has problems of slow response, high power consumption, and long operation time. To solve these problems, this study focuses on the optimization of hot standby operations for the EB system. First, mathematical models of an electrode immersion EB and its accompanying deaerator were established. Then, a control simulation platform of the EB system was developed in MATLAB/Simulink implementing the established mathematical models and corresponding control systems. Finally, two optimization strategies for the EB hot standby operation were proposed, followed by dynamic simulations of the EB system transient from hot standby to normal operations. The results indicate that the proposed optimization strategies can significantly speed up the transient response of the EB system from hot standby to normal operations and reduce the power consumption in hot standby operations, improving the dynamic performance and economy of the system.

Visible-light driven efficient elimination of organic hazardous and Cr (VI) over BiOCl modified by Chinese Baijiu distillers’ grain-based biochar

Xingyun Jin,Xiaolong Tang,Huan Li,Xiaoqian Tang,Jian zhang Li,Jun bo Zhong,Shulin Zhang,Dongge Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

In this paper, distillers’ grains biochar (DGBC) was facilely gained by processing the distillers’ grains producedin Chinese Baijiu brewing, and the DGBC was used to modify BiOCl. The prepared DGBC/BiOCl compositephotocatalysts show elevated visible light-driven catalytic performance. X-ray diffraction (XRD)and scanning electron microscope (SEM) suggest the presence of DGBC affects the crystal growth andmicroscopic morphology of BiOCl. The low-temperature electron paramagnetic resonance (EPR) testaffirms more oxygen vacancies (OVs) exist in the DGBC/BiOCl composite photocatalysts. The 0.75-DGBC/BOC photocatalyst holds the highest efficiency for removal of rhodamine B (RhB), tetracycline(TC) and Cr (VI) compared with BiOCl, commercial TiO2 (P25) and other DGBC/BiOCl composites. Giventhe experimental results, the enhanced visible light responsive performance originates from the richOVs introduced by the modification of BiOCl with DGBC and the higher separation rate of photoexcitedcarriers.

Mechanistic Insight into Phenolic Compounds Toxicity and State-of-the-art Strategies for Enhancing the Tolerance of Escherichia coli to Phenolic Compounds

Lina Liu,Xiaolong Ma,Muhammad Bilal,Linlin Wei,Shijie Tang,Hong-zhen Luo,Yuping Zhao,Xuguo Duan 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

Efficient use of lignocellulosic biomass is a prerequisite for sustainable biofuel production while simultaneously contributing to environmental protection. However, phenolic compounds produced during the chemical treatment of lignocellulose inhibit the growth and metabolism of microorganisms, such as Escherichia coli, which is one of the ideal strains for producing target products by microbial fermentation. To provide new ideas for studying microbial tolerance to environmental stress and providing technical support for constructing the engineering strains with high yields of phenolic compounds, this review elucidates the inhibition mechanism of phenols to E. coli. Secondly, a comprehensive and systematic review of current approaches for improving the phenolic-tolerance of E. coli is provided, including strain domestication, random mutagenesis, regulating the expression of outer membrane proteins, changing the composition of membrane fatty acids, accelerating the efflux of phenolic compounds, engineered bacterial biofloc formation, and transcriptome analysis. Finally, this review ends with some questions that still exist today, and prospective strategies are outlined for further improving the phenols-tolerance of E. coli. This review provided a theoretical basis for research into microbial tolerance to environmental stress and the development of engineered strains with high yield of phenolic compounds.

A Novel Modulation Strategy for Diode-assisted Buck-boost VSI with Maximum Voltage Gain and Minimum Switching Frequency

Yan Zhang,Xiaolong Ma,Jinjun Liu,Zhuo Dong,Shah Zaman,Jiuqiang Han 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Diode-assisted buck-boost voltage source inverter (VSI) boosts the dc source voltage by introducing a diode-capacitor network. With capacitive charging in parallel and discharging in series, it extends voltage regulation range and avoids extreme boost duty ratio. As for the unique structure, various pulse width modulation (PWM) strategies are provided with regarding to the chopped intermediate dc-link voltage. In order to maximize voltage gain, as well as to achieve the increased efficiency, this paper proposed a novel modulation strategy by reducing the switching frequency of power devices in the inverter bridge. The operation principle and relationship of switching device voltage stress versus voltage gain are analyzed in detail. Simulation and experiments are implemented to verify the theoretical analysis. Compared with existing modulation strategies, diode-assisted buck? boost VSI with new modulation scheme demonstrates good performances: less power device requirement and higher efficiency in high voltage gain application. Owing to improved performance, it is a more competitive topology for wide range dc/ac voltage regulation in renewable energy application, especially for ac output with relatively high fundamental frequency. Furthermore, the idea of proposed modulation scheme can also be extended to the control of other quasi two-stage inverter.

Combined adsorption and reduction of Cr(VI) from aqueous solution on polyaniline/multiwalled carbon nanotubes composite

Jiahong Wang,Xiaolong Yin,Wei Tang,Hongrui Ma 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.9

Polyaniline/multiwalled carbon nanotube (PANI-MWCNT) was prepared by bounding polyaniline on the surface of oxidized multiwalled carbon nanotube. The structure and surface properties of synthesized composites were characterized by Fourier transformed infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), transmission electron microscope (TEM), and its adsorption capability for aqueous Cr(VI) was also studied. Characterized results showed that polyaniline was successfully anchored on the surface of MWCNT. From adsorption experiments the maximum adsorption amount of Cr(VI) onto PANI-MWCNTs was 28.25, 31.75 and 36.76 mg·g−1 at 15, 25 and 35 oC. Thermodynamic parameters showed that the Cr(VI) adsorption process was endothermic, spontaneous and feasible. Cr(VI) adsorption followed pseudo-second-order kinetics. Cr(VI) adsorption on the adsorbent decreases with increasing solution pH. The presence of anions in solution almost has no effect on Cr(VI) adsorption, indicating good selectivity. XPS analysis confirms that electrostatic interaction, reduction and chelation contribute to enhanced Cr(VI) removal. Cr(VI) loaded absorbent can be readily desorbed in 0.1mol·L−1 of NaOH solution, and the desorption rate was 84.12%.

An Improved Control Strategy for Z-source Inverter with Maximum Boost Capability and Minimum Switching Frequency

Yan Zhang,Xiaolong Ma,Jinjun Liu,Zhuo Dong,Jiuqiang Han 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Z-source inverter introduces a unique Xshaped impedance network and boosts the input voltage by utilizing shoot-through (ST) states. It provides a competitive single-stage approach for DC to AC power conversion in relatively low voltage boost ratio range (1?2). This paper proposes a novel control strategy for Z-source inverter. With this strategy, only one phase leg operates at high switching frequency to achieve both ST duty ratio control and output voltage regulation, while the other two phase legs maintain the fixed switching states during one sextant. Therefore, it reduces the equivalent switching frequency of power devices in the inverter bridge to about 1/3. However, it introduces the low frequency current and voltage ripple in the inductor and capacitor which related to the fundamental frequency of output voltage. It has the potential to achieve higher efficiency especially in high-switching-frequency and medium-frequency DC-AC power application. Then, the paper provides a detailed close-loop controller design approach for the novel modulation strategy. Furthermore, the detailed comparisons among the existing strategies and proposed control strategy are conducted in theory. Finally, all the theoretical analysis and calculation are verified by simulation results.

Genome Sequencing Highlights the Plant Cell Wall Degrading Capacity of Edible Mushroom Stropharia rugosoannulata

Guo Mengpei,Ma Xiaolong,Zhou Yan,Bian Yinbing,Liu Gaolei,Cai Yingli,Huang Tianji,Dong Hongxia,Cai Dingjun,Wan Xueji,Wang Zhihong,Xiao Yang,Kang Heng 한국미생물학회 2023 The journal of microbiology Vol.61 No.1

The basidiomycetous edible mushroom Stropharia rugosoannulata has excellent nutrition, medicine, bioremediation, and biocontrol properties. S. rugosoannulata has been widely and easily cultivated using agricultural by-products showing strong lignocellulose degradation capacity. However, the unavailable high-quality genome information has hindered the research on gene function and molecular breeding of S. rugosoannulata. This study provided a high-quality genome assembly and annotation from S. rugosoannulata monokaryotic strain QGU27 based on combined Illumina-Nanopore data. The genome size was about 47.97 Mb and consisted of 20 scaffolds, with an N50 of 3.73 Mb and a GC content of 47.9%. The repetitive sequences accounted for 17.41% of the genome, mostly long terminal repeats (LTRs). A total of 15,726 coding gene sequences were putatively identified with the BUSCO score of 98.7%. There are 142 genes encoding plant cell wall degrading enzymes (PCWDEs) in the genome, and 52, 39, 30, 11, 8, and 2 genes related to lignin, cellulose, hemicellulose, pectin, chitin, and cutin degradation, respectively. Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation.

Comparison of Conventional DC-DC Converter and a Family of Diode-Assisted DC-DC Converter in Renewable Energy Applications

Zhang, Yan,Liu, Jinjun,Ma, Xiaolong,Feng, Junjie The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.2

In the conventional dc-dc converter, a pair of additional diode and the adjacent passive component capacitor/inductor can be added to the circuit with an X-shape connection, which generates a family of new topologies. The novel circuits, also called diode-assisted dc-dc converter, enhance the voltage boost/buck capability and have a great potential for high step-up/step-down power conversions. This paper mainly investigates and compares conventional dc-dc converter and diode-assisted dc-dc converter in wide range power conversion from the aspects of silicon devices, passive components requirements, electro-magnetic interference (EMI) and efficiency. Then, a comprehensive comparison example of a high step-up power conversion system was carried out. The two kinds of boost dc-dc converters operate under the same operation conditions. Mathematical analysis and experiment results verify that diode-assisted dc-dc converters are very promising for simultaneous high efficiency and high step-up/step-down power conversion in distributed power supply systems.

Comparison of Conventional DC-DC Converter and a Family of Diode-Assisted DC-DC Converter in Renewable Energy Applications

Yan Zhang,Jinjun Liu,Xiaolong Ma,Junjie Feng 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.2

In the conventional dc-dc converter, a pair of additional diode and the adjacent passive component capacitor/inductor can be added to the circuit with an X-shape connection, which generates a family of new topologies. The novel circuits, also called diode-assisted dc-dc converter, enhance the voltage boost/buck capability and have a great potential for high step-up/step-down power conversions. This paper mainly investigates and compares conventional dc-dc converter and diode-assisted dc-dc converter in wide range power conversion from the aspects of silicon devices, passive components requirements, electro-magnetic interference (EMI) and efficiency. Then, a comprehensive comparison example of a high step-up power conversion system was carried out. The two kinds of boost dc-dc converters operate under the same operation conditions. Mathematical analysis and experiment results verify that diode-assisted dc-dc converters are very promising for simultaneous high efficiency and high step-up/step-down power conversion in distributed power supply systems.