Inhibition of Invasion and Metastasis of MHCC97H Cells by Expression of Snake Venom Cystatin through Reduction of Proteinases Activity and Epithelial-Mesenchymal Transition

Nanhong Tang,Qun Xie,Xiaoqian Wang,Xiujin Li,Yanlin Chen,Xu Lin,Jianyin Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.5

Snake venom cystatin (sv-cystatin) is a member of the cystatin family of cysteine protease inhibitors. To further evaluate the possibility of sv-cystatin in cancer therapy, this study examined the effects of sv-cystatin on the invasion and metastasis of liver cancer cells (MHCC97H) in vitro and in vivo as well as the underlying mechanism. sv-cystatin cDNA was transfected into MHCC97H cells and the anti-invasion and antimetastasis effects of sv-cystatin were determined using migration and matrigel invasion assays and a lung-metastasis mice model. The results suggest that sv-cyst clone (sv-cystatin expression in MHCC97H cells) delayed the invasion and metastasis in vitro and in vivo compared to the parental, mock and si-sv-cyst clone cells (inhibited sv-cystatin expression by siRNA). The decreased activities of cathepsin B, MMP-2 and MMP-9 and EMT change index including higher E-cadherin, lower N-cadherin and decreased Twist activity were observed in the sv-cyst clone, which contributes to the change in invasion and metastasis ability of MHCC97H cells. This study provides evidence that expression of the sv-cystatin gene in MHCC97H cells inhibits tumor cell invasion and metastasis through the reduction of the proteinases activity and Epithelial-Mesenchymal Transition (EMT), which might contribute to the anticancer research of the sv-cystatin protein.

Does matching relation exist between the length and the tilting angle of terminal implants in the all-on-four protocol? stress distributions by 3D finite element analysis

Zhen Tang,Lulu Gong,Dalin Wang,Xiaoqian Qiu,Zhizhong Cao,Xiaomei Li 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.3

PURPOSE To explore whether there is matching relation between the length and the tilting angle of terminal implants in the All-on-Four protocol by studying the effects of different implant configurations on stress distributions of implant, bone, and framework. MATERIALS AND METHODS Four implants were employed to support a full-arch fixed prosthesis and five three-dimensional finite element models were established with CT images, based on the length (S and L) and distal tilt angle (0°, 30° and 45°) of terminal implants for an edentulous mandible, which named: Tilt0-S, Tilt30-S, Tilt30-L, Tilt45-S and Tilt45-L. An oblique 240 N was loaded at second molar. The von Mises Stresses were analyzed. The implants were consecutively named #1 to #4 from the loading point. RESULTS 1) Tilt0-S had the greatest stress on the implants, with the other groups exhibiting variable reductions; the four implants of Tilt45-L demonstrated the greatest reduction in stress. 2) Tilt0-S had the greatest stress at bone around #1 implant neck, and Tilt45-L exhibited the least stress, which was a 36.3% reduction compared to Tilt0-S. 3) The greatest stress in the framework was found on the cantilevers distal to #1 implant. Tilt45-S exhibited the least stress. CONCLUSION Matching different length and tilting angle of the terminal implants led to variable stress reductions on implants, bone and the superstructure. By optimizing implant configuration, the reduction of stress on implants and surrounding bone could be maximized. Under the present condition, Tilt45-L was the preferred configuration. Further clinical testings are required.

Visible-light driven efficient elimination of organic hazardous and Cr (VI) over BiOCl modified by Chinese Baijiu distillers’ grain-based biochar

Xingyun Jin,Xiaolong Tang,Huan Li,Xiaoqian Tang,Jian zhang Li,Jun bo Zhong,Shulin Zhang,Dongge Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

In this paper, distillers’ grains biochar (DGBC) was facilely gained by processing the distillers’ grains producedin Chinese Baijiu brewing, and the DGBC was used to modify BiOCl. The prepared DGBC/BiOCl compositephotocatalysts show elevated visible light-driven catalytic performance. X-ray diffraction (XRD)and scanning electron microscope (SEM) suggest the presence of DGBC affects the crystal growth andmicroscopic morphology of BiOCl. The low-temperature electron paramagnetic resonance (EPR) testaffirms more oxygen vacancies (OVs) exist in the DGBC/BiOCl composite photocatalysts. The 0.75-DGBC/BOC photocatalyst holds the highest efficiency for removal of rhodamine B (RhB), tetracycline(TC) and Cr (VI) compared with BiOCl, commercial TiO2 (P25) and other DGBC/BiOCl composites. Giventhe experimental results, the enhanced visible light responsive performance originates from the richOVs introduced by the modification of BiOCl with DGBC and the higher separation rate of photoexcitedcarriers.

Catalytic pyrolysis of corncob with Ni/CaO catalysts for hydrogen-rich gas: Synthesis modes and catalyst/biomass ratios

Hongyu Liu,Yuting Tang,Xiaoqian Ma,Wenchang Yue 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

With the depletion of traditional fossil fuels, biomass has evolved as a new environmentally friendly andsustainable alternative energy source. Ni/CaO catalysts are promising for CO2 capture and biomass catalyticuse. In this study, we examined four different synthesis modes for Ni/CaO catalysts: impregnationmethod, sol–gel method (citric acid complexation), sol–gel method with propionic acid modification andsedimentation method, and used the catalysts in the pyrolysis of corncob to produce hydrogen-rich gas at600 C. According to the results of the fixed-bed experiments, adding the propionic acid modification tothe citric acid complexation boosted hydrogen generation by 24.93 vol.%. The global behavior of the catalystssynthesized by the different methods follows the order sol-Ni1/Ca7-P > sol-Ni1/Ca7 > sed-Ni1/Ca7imp-Ni1/Ca7-A. At a catalyst/biomass ratio of 2:1, the maximum H2 concentration of 84.45 ± 1.02vol.% and H2 production of 26.84 ± 1.26 mmol/gbiomass were attained, as well as CO2 concentrations of just2.08 ± 0.03 vol.%. The H2 production was about 20 times higher than without the addition of the catalyst. Optimizing the synthesis modes and catalyst/biomass ratio produced high-quality hydrogen-rich gaswith a high H2 conversion of 88.95 ± 2.20 % and the energy efficiency of 52.73 %.

Does matching relation exist between the length and the tilting angle of terminal implants in the all-on-four protocol? stress distributions by 3D finite element analysis

Li, Xiaomei,Cao, Zhizhong,Qiu, Xiaoqian,Tang, Zhen,Gong, Lulu,Wang, Dalin The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.3

PURPOSE. To explore whether there is matching relation between the length and the tilting angle of terminal implants in the All-on-Four protocol by studying the effects of different implant configurations on stress distributions of implant, bone, and framework. MATERIALS AND METHODS. Four implants were employed to support a full-arch fixed prosthesis and five three-dimensional finite element models were established with CT images, based on the length (S and L) and distal tilt angle ($0^{\circ}$, $30^{\circ}$ and $45^{\circ}$) of terminal implants for an edentulous mandible, which named: Tilt0-S, Tilt30-S, Tilt30-L, Tilt45-S and Tilt45-L. An oblique 240 N was loaded at second molar. The von Mises Stresses were analyzed. The implants were consecutively named #1 to #4 from the loading point. RESULTS. 1) Tilt0-S had the greatest stress on the implants, with the other groups exhibiting variable reductions; the four implants of Tilt45-L demonstrated the greatest reduction in stress. 2) Tilt0-S had the greatest stress at bone around #1 implant neck, and Tilt45-L exhibited the least stress, which was a 36.3% reduction compared to Tilt0-S. 3) The greatest stress in the framework was found on the cantilevers distal to #1 implant. Tilt45-S exhibited the least stress. CONCLUSION. Matching different length and tilting angle of the terminal implants led to variable stress reductions on implants, bone and the superstructure. By optimizing implant configuration, the reduction of stress on implants and surrounding bone could be maximized. Under the present condition, Tilt45-L was the preferred configuration. Further clinical testings are required.

HEALER: homomorphic computation of ExAct Logistic rEgRession for secure rare disease variants analysis in GWAS

Wang, Shuang,Zhang, Yuchen,Dai, Wenrui,Lauter, Kristin,Kim, Miran,Tang, Yuzhe,Xiong, Hongkai,Jiang, Xiaoqian Oxford University Press 2016 Bioinformatics Vol.32 No.2

<P>Motivation: Genome-wide association studies (GWAS) have been widely used in discovering the association between genotypes and phenotypes. Human genome data contain valuable but highly sensitive information. Unprotected disclosure of such information might put individual's privacy at risk. It is important to protect human genome data. Exact logistic regression is a bias-reduction method based on a penalized likelihood to discover rare variants that are associated with disease susceptibility. We propose the HEALER framework to facilitate secure rare variants analysis with a small sample size. Results: We target at the algorithm design aiming at reducing the computational and storage costs to learn a homomorphic exact logistic regression model (i.e. evaluate P-values of coefficients), where the circuit depth is proportional to the logarithmic scale of data size. We evaluate the algorithm performance using rare Kawasaki Disease datasets.</P>