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Genome wide methylation analysis to uncover genes related to recurrent pregnancy loss
Lixia Zhou,Yudong Pu,Yuxun Zhou,Bin Wang,Ye Chen,Yang Bai,Shuzhen He 한국유전학회 2021 Genes & Genomics Vol.43 No.4
Background Recurrent pregnancy loss (RPL) refers to two or more consecutive spontaneous abortion before 24 weeks of gestation, representing 1% of couples of childbearing age. Epigenetic factors including dysregulation of DNA methylation of some genes may play a role in RPL. Objective To identify RPL related genes modulated by DNA methylation expressed in decidua and blood. Methods Three decidua samples each from RPL patients and normal controls were recruited to perform genome-wide bisulfte sequencing (GWBS) and transcriptome sequencing. Based on the above results, 22.52 kb of diferential methylation regions (DMRs) from 17 genes were verifed by bisulfte sequencing PCR at specifc region (Hi-MethylSeq) in another 15 decidua (7RPL vs. 8 Controls) and 13 blood (5RPL vs. 8 Controls) samples. Results 23 genes showed signifcantly diferential cytosine methylation status and distinct expression level between PRL patients and healthy controls synergistically. Three signaling pathways were found to be shared between genes with both hypomethylated diferential methylation regions (DMR) and upregulated diferential gene expression (DGE). The results from Hi-MethylSeq showed that the hypermethylation of SGK1 in both blood and decidua samples in RPL patients, which was consistent to its lower expression in endometrium reported earlier. SGK3 and CREB5 also showed modulated methylation level in RPL decidua. Conclusion Our fnding supported that aberrant methylation of SGK1 and CREB5 could be a cause of the dysregulation of these gens in the endometrium, which is one of cause of reproductive failure. The function of SGK3 in reproduction system deserves further investigation.
Socially Aware Device-to-multi-device User Grouping for Popular Content Distribution
( Jianlong Liu ),( Wen’an Zhou ),( Lixia Lin ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.11
The distribution of popular videos incurs a large amount of traffic at the base stations (BS) of networks. Device-to-multi-device (D2MD) communication has emerged an efficient radio access technology for offloading BS traffic in recent years. However, traditional studies have focused on synchronous user requests whereas asynchronous user requests are more common. Hence, offloading BS traffic in case of asynchronous user requests while considering their time-varying characteristics and the quality of experience (QoE) of video request users (VRUs) is a pressing problem. This paper uses social stability (SS) and video loading duration (VLD)-tolerant property to group VRUs and seed users (SUs) to offload BS traffic. We define the average amount of data transmission (AADT) to measure the network’s capacity for offloading BS traffic. Based on this, we formulate a time-varying bipartite graph matching optimization problem. We decouple the problem into two subproblems which can be solved separately in terms of time and space. Then, we propose the socially aware D2MD user selection (SA-D2MD-S) algorithm based on finite horizon optimal stopping theory, and propose the SA-D2MD user matching (SA-D2MD-M) algorithm to solve the two subproblems. The results of simulations show that our algorithms outperform prevalent algorithms.
UBE2W Interacts with FANCL and Regulates the Monoubiquitination of Fanconi Anemia Protein FANCD2
Yingying Zhang,Peitang Huang,Xiaowei Zhou,Lixia Zhao,Chao Li,Hengqi Zhu,Long Xu,Liran Shan,Xiang Liao,Zekun Guo 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2
Fanconi anemia (FA) is a rare cancer-predisposing ge-netic disease mostly caused by improper regulation of the monoubiquitination of Fanconi anemia complementation group D2 (FANCD2). Genetic studies have indicated that ubiquitin conjugating enzyme UBE2T and HHR6 could regulate FANCD2 monoubiquitination through distinct mechanisms. However, the exact regulation mechanisms of FANCD2 monoubiquitination in response to different DNA damages remain unclear. Here we report that UBE2W, a new ubiquitin conjugating enzyme, could regulate FANCD2 monoubiquitination by mechanisms different from UBE2T or HHR6. Indeed, UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL) in vitro. UBE2W binds to FANCL, and the PHD domain is both necessary and sufficient for this interaction in mammalian cells. In addition, over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradia-tion-induced but not MMC-induced FANCD2 monoubiquiti-nation. These results indicate that UBE2W regulates FANCD2 monoubiquitination by mechanisms different from UBE2T and HRR6. It may provide an additional regulatory step in the activation of the FA pathway.
Effect of the worn status of wheel/rail profiles on wheel wear over curved tracks
Jie Kou,Jimin Zhang,Hechao Zhou,Chengping Wang,Lixia Sun 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.3
The worn status of wheel/rail profiles could change the wheel-rail contact and influence the wear rates of the wheel and rail. Worn wheel profiles of a Chinese intercity train and the worn rail profile of curved tracks were taken into account in this work. According to the dynamics model of the vehicle, teh wheel-rail three-dimensional steady-state rolling contact model, and the Archard wear model, a new fast calculation method of wheel wear rate was proposed. Simulation results show that obviously severe wear would distribute in the flange when the wheel and rail were worn. The side wear of rail gauge corner will seriously aggravate the wear of new wheel flange. The wear of the wheel is serious for teh worn wheel in the initial phase of service. Therefore, the re-profiling of wheels and rails should be scheduled cooperatively, and the wear of flange in the initial phase of service should addressed.
Peng, Xu,Yu, Le,Gong, Kaiyue,Liu, Xiaohan,Yang, Guolong,Xu, Jiangjun,Zhou, Chao,Gao, Lixia,Zhu, Xinyu The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.8
An aircraft ground power unit (GPU) can replace the auxiliary power unit (APU) in an aircraft while the aircraft is parked at an airport. Therefore, the problems associated with the oil consumption and air pollution of the APU are solved. However, the GPU is faced with challenges in terms of utilization, power quality, and fault tolerance. Thus, a novel centralized aircraft GPU based on a three-level neutral point clamped cascaded converter (3LNPC-CC) is introduced in this paper to improve utilization and power quality. Furthermore, an evaluation voltage balance strategy is proposed for the DC-port fault tolerance of the 3LNPC-CC. In addition, both the modulation of the phase shift pulse width modulation (PSPWM) and the control of the double closed loop are used in the 3LNPC-CC. A simulation platform including the control, modulation, and fault tolerance of the system is established to verify the feasibility and performance of the proposed GPU. In particular, it is used to evaluate the voltage balance strategy for DC-port faults. Moreover, a prototype of the GPU and relevant experiments are completed to verify the correctness and feasibility of the system and the strategy.
Lu Yunjun,Li Shu,Shen Xiaodong,Zhao Yan,Zhou Dongming,Hu Dan,Cai Xushen,Lu Lixia,Xiong Xiaohui,Li Ming,Cao Min 한국미생물학회 2021 The journal of microbiology Vol.59 No.10
Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that presents a significant threat both to pigs and to workers in the pork industry. The initial steps of S. suis 2 pathogenesis are unclear. In this study, we found that the type II histidine triad protein HtpsC from the highly virulent Chinese isolate 05ZYH33 is structurally similar to internalin A (InlA) from Listeria monocytogenes, which plays an important role in mediating listerial invasion of epithelial cells. To determine if HtpsC and InlA function similarly, an isogenic htpsC mutant (ΔhtpsC) was generated in S. suis by homologous recombination. The htpsC deletion strain exhibited a diminished ability to adhere to and invade epithelial cells from different sources. Double immunofluorescence microscopy also revealed reduced survival of the ΔhtpsC mutant after cocultivation with epithelium. Adhesion to epithelium and invasion by the wild type strain was inhibited by a monoclonal antibody against E-cadherin. In contrast, the htpsC-deficient mutant was unaffected by the same treatment, suggesting that E-cadherin is the host-cell receptor that interacts with HtpsC and facilitates bacterial internalization. Based on these results, we propose that HtpsC is involved in the process by which S. suis 2 penetrates host epithelial cells, and that this protein is an important virulence factor associated with cell adhesion and invasion.
Guangming Zheng,Zhenying Huang,Qun Yu,Wenqiang Hu,Xingyang Qiu,A. Lixia,Yuanbo Wang,Yidan Jiao,Yang Zhou,Hongxiang Zhai 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.6
In this paper, a novel type of functionally graded material (FGM) was successfully fabricated from pure Ni and Ti3AlC2powder mixtures by hot-press sintering route at 1200 °C and 17 MPa. In each layer, Ti3AlC2particles transformed into TiCxphase, while the additional Al–Ti atoms decomposed from Ti3AlC2diffused into the Ni matrix, giving rise to the formationof γ′-Ni3(Al,Ti). Part of the submicron TiCxand Ni3(Al,Ti) particles were uniformly distributed in the Ni matrix, whilesome of the TiCxmaintained the original Ti3AlC2plate-like morphology. Both TiCxand Ni3(Al,Ti) content decreased from60Ti3AlC2/Ni layer to 10Ti3AlC2/Ni layer in a stepwise manner was fabricated. The microstructural analysis indicatesthat no cracks, delamination and step-type abrupt changes occured in the graded layer of the manufactured FGM. Flexuralstrength and Vickers hardness enhanced with the increasing Ti3AlC2content except for fracture strain and toughness. Theoverall fracture toughness and the flexural strength of FGM can reach 21.76 MPa m1/2 and 1329 ± 34 MPa, respectively. Theprepared FGM, herein, with a hard ceramic surface on one side to resist local plastic deformation, and a ductile metallicsurface on the other side to provide toughness.