Identification of Novel Endo-β-1,4-glucanase Isoforms fromBursaphelenchus Species (Nemtoda: Aphelenchoididae)

Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2

Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.

갑상선암 예측에 있어 초음파적 변수 분석

임현정,최영식,박요한,오경승,이강대 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Background: The introduction of high resolution sonography has made it possible to detect nodules in the thyroid gland. However, there has been no reliable sonographic sign for distinguishing between benign and malignant thyroid conditions. The aim of present study was to evaluate ultrasonographic (US) parameters in predicting thyroid cancer. Methods: One hundred fifty three patients who underwent thyroidectomy at Kosin Medical Center from January, 2000 to April, 2002 were included in the study. All patients were checked high resolutional US before surgery. Of the 153 patients 89 were malignant tumors and 64 benign. Malignant US parameters were defined as calcification, irregular margin, more taller than wide, and fat line obliteration. Calcification pattern was classified with single, punctated, and amorphous calcification. The US characteristics to predict malignancy were evaluated by means of multiple logistic regression analysis. Results: All of the malignant sonographic parameters such as calcification, 53 (89.8%) were thyroid carcinoma. The incidence rate of malignancy was 55.9% in solitary nodules and 65.7% in multiple nodules (p=0.303). Even though nodule size was less than 1.5㎝, the incidence of malignant tumor was 69.5% (p=0.04). Of 53 malignant nodules with calcification, the incidence of single, punctated, and amorphous calcification pattern was 6 (11.3%), 22 (41.5%), 25(47.2%), respectively. The sensitivity, specificity, positive and negative predictive value, and accuracy of sonographic parameters were 76.4%, 87.5%, 89.5%, 72.7% and 81.0%, respectively. Upon the correlation of US-guided FNA cytology with pathologic diagnosis, the sensitivity of ultrasound-guided FNA cytology in differentiating benign and malignant nodule was 85.9%, the specificity 100% and overall diagnostic accuracy was 91.2%. The sensitivity, specificity, and accuracy of US parameters associated to follicular neoplasm, all were 75.0%. Conclusion : All the malignant US parameters including calcification, irregular margin, and more tall than wide showed high level of sensitivity, specificity and accuracy. Of these parameter, calcification and irregular margin were more significants. The more presence of calcifications with punctated and amorphous pattern could suggest the higher possibility of malignancy. However, further study is needed for clinical application of malignant US parameters in the thyroid tumors.

Zr/Ti 비에 따른 PZT계 압전세라믹스의 온도안정성

임대관,현원석,이개명 濟州大學校 情報通信硏究所 1998 情報通信硏究所論文集 Vol.1 No.-

In this paper, temperature stabilities of Pb( Zr_xTi_(1-x))O₃ piezoelectric ceramic system according to Zr/Ti ratios were investigated. PZT ceramics with Zr/Ti =58/42, 56/44, 53/47, 50/50, 48/52 which are adjacent compositions to Mophotyophic phase were fabricated, and thermal aging effects on the specimen were measured. After thermal aging. the specimens with Zr/Ti=58/42, 56/44, 48/52 in them could have lower temperature coefficient for antiresonant frequency and the specimen with Zr/Ti = 58/42 which is a composition of Rhombohedral phase have had the lowest temperature coefficient for antiresonant frequency.

하악골 비대칭과 측모두부방사선사진상 이중영상 간의 관계

임현대,한경수 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.2

This study was performed to investigate the affecting factors to double images on the cephalometric radiograph. For this study, 28 patients with temporomandibular disorders and 31 healthy dental students without any signs and symptoms of temporomandibular disorders were selected, and the cephalometric and the panoramic radiographs were taken. The observed and measured points related to double images on the cephalometric radiograph were Or, Go1, Go2, Go3, and C1. Both mandibular ramus height were measured on the panoramic radiograph. Natural head and body posture in the frontal and the sagittal plane were observed with CROM?? and with posture measuring board, with regard to several parameters. The data obtained were processed with SPSS Windows?? program. The result were as follows : 1. There were no correlation between the difference of both mandibular ramus height and the length of cephalometric double image at observed points. 2. The length of double image in Or and Go2 in the subjects with more headtilt in frontal plane(≥2˚ )were different from the subjects with less headtilt(≤1˚ ). 3. Difference of angulation of both sternocleidomastoid muscles in sagittal plane did not affect the length of cephalometric double image in all the measured points. 4. There were negative correlation between the length of cephalometric double image in Go1 and C1 and canthotragus angle. 5. There were no correlation between the length of cephalometric double image and the frontal parameters, but there were significant correlation between the length of double image and the sagittal parameters such as sagittal angle, degree of forward head posture(FHP), and canthotragus angle.

채용시 건강진단에서 요ㆍ천추부 단순 방사선 검사의 평가

임현술,김수군,김덕수,이현경,최대섭 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : A study was conducted for investigating the status of simple X-rays on lumbo-sacral regions at pre-employment health examination and analysing the effectiveness of simple X-rays on lumbo-sacral regions. Methods : The study data were pre-employment health examination data in a university hospital from Jan 3, 1993 through October of 1997. And, 97 newly employed workers who claimed no low back pawn at pre-employment health examination of a certain company were follow-up survey about low back pain and related factors in April, 1995 and October of 1997. The data were collected by a self-reported questionnaire and a medical examination with a rehabilitation specialist. Results : 1. Taking the simple X-rays on lumbo-sacral regions was 1,591 workers (10.5%) among 15,166 pre-employment health examination from Jan 3, 1993 through October of 1997. And yearly application rates of these X-ray tests have been significantly statistical increasing (p〈0.01). 2. The abnormal findings of simple X-rays on lumbo-sacral regions at pre-employment health examination were 40 workers. But, there was not significantly statistical difference between normal and abnormal group for the complaint rate of low back pain. 3. The number of case with symptoms of low back pain at the follow-up survey were 46, so The incidence density was 37.1 persons/ 100 person-years. And the incidence density was not significantly statistical difference between normal and abnormal group of simple X-rays on lumbo-sacral regions. 4. Through the multivariate logistic regression, significantly associated factors with low back Pain were found to be tenure(OR=0.36, 95%CI : 0.17-0.79), lifting of heavy materials (OR=5.86, 95%CI : 1.58-21.74) . Conclusion : The simple X-rays on lumbo-sacral regions at pre-employment health examination is required further research for utilizing pre-employment health examination according to above results.

자동 경계 탐지(Automated Border Detection)심초음파도를 이용한 좌심방 면적 및 기능의 평가

김현주,주승재,임진형,정효균,이영수,유호대,차태준,이재우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.2

생활폐기물을 이용한 세라믹스 판재 개발

신대용,임현태,최돈량 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

생활하수처리장에서 발생하는 하수슬러지의 용융슬래그와 장석, 물유리 및 전분을 이용하여 세라믹스 다공판을 제조하였다. 슬래그의 주성분은 SiO_2와 Al_2O_3로서 점토와 유사한 성질을 갖고 있어 세라믹스의 대체원료로 사용이 가능하였다. 하수슬러지를 1,200℃에서 2시간 열처리하여 얻어진 용융물을 수증에 급랭시켜 1∼3mm 입경의 슬래그 분말을 제조하고 80∼95wt%의 슬래그, 5∼20wt%의 장석, 5∼15wt%의 물유리 및 2wt%의 전분을 혼합하여 1,100∼1,200℃에서 2시간 소성하여 시편을 제조하였다. 슬래그의 첨가량과 소성온도가 증가함에 따라 시편의 부피비중 및 압축강도는 증가하였으나, 기공률과 흡수율은 감소하였다. 1,030℃에서 2시간 소성한 No. 1, 4 및 7번 시편은 부피비중 1.52∼1.67, 기공률과 흡수율 23.3∼ 32.5% 및 14.1∼19.3%, 압축강도 81∼173kgf/㎠의 값을 나타내었다. 또한, 슬래그의 첨가량과 소성온도가 동등한 시편은 물유리의 첨가량이 증가함에 따라 부피비중과 압축강도가 증가하여 No.7, 8 및 9번 시편은 부피비중 1.52∼1.94, 압축강도 81∼385kgf/㎠의 값을 나타내었다. 시편의 중금속용출량은 미량으로서 매립지 침출수배출 허용기준치보다 낮게 측정되어 주위환경에 해가 없고 흡음특성이 양호하여 본 연구에서 제조한 세라믹스 다공판은 흡음판으로의 이용이 가능하였다. Utilization of sewage sludge from the sewage sludge treatment facilities and the municipal wastes became very important matter to be solve for the environmental protection and recycling of waste materials. The reusability of sewage sludge slag as a raw material in the manufacturing process of ceramics was highly expected because the chemical composition of slag was mostly SiO_2 and Al_2O_3 and its properties were very similar with clay. Sewage sludge slag was fabricated by sintering at 1,200℃ for 2 hours and it was crushed into powder with particle size of 1∼3mm by a rapid cooling treatment in water. Then, the mixture of 80∼95 wt% of slag, 5∼20 wt% of feldspar, 5∼15 wt% of water glass and 2 wt% of starch were heated at 1,000∼1,100℃ for 2 hours. The density and compressive strength of specimen increased with increasing the sintering temperature and the addition amount of slag, No. 1, 4 and 7, specimens were shown 1.52∼1.67 of bulk density, 23.3∼32.5% of porosity, 14.1∼19.3% of water absorption and 81∼173 kgf/㎠ of compressive strength. And, that of the specimen at the same sintering temperature and addition amount of slag increased with increasing the addition amount of water glass, No. 7, 8 and 9 specimens, bulk density was 1.52∼1.94 and the compressive strength was 81∼385kgf/㎠. An extraction test for specimen to detect the toxic materials, such as Cu, Cd, Cr, Pb and As, showed below the permitted standard of land-filling extractable heavy metal contents. Sound absorption coefficients of specimen had a good properties in No. 7 specimen, and can be used as a sound absorption plate.

수종 유기산 용액에서 글라스 아이오노머 시멘트의 표면조도 및 색 변화에 관한 연구

김용대,문현정,임범순,김철위 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

The chemical bonding ability of glass ionomer cement to tooth structure and sustained fluoried release render glass ionomer cements advantageous over other restorative materials, such a amalgam and composite resins. Poor polishability and high solubility in organic solvents, however, are still a significant disadvantage of the existing glass ionomer cements. The purpose of this study was to estimate the aging effect of glass ionomer cements in organic acid solutions on surface change and color change. Using metal molds, disk type speciments, 100mm×1mm were prepared from three chemical-curing and three light-curing glass ionomer cements. Specimens were prepared from each material following the manufacturer's intructions, and setted against a mylay matrix strip supported by a glass slab. Three specimens were prepared for each experimental groups. Profilometric analyses were carried out using the surface roughness tester(Surtronic 3P, Rank Taylor Hobson Ltd. England). R?? valuse, which were the mathematical mean values of the departure of the roughness profile from the mean line calculated by the machine, recorded. Color measurements(CIE coefficients ; L, a, b) were peformed using the spectrophotometer(CM-3500d, Minolta, Japan). Before aging treatment and after 1, 7, 14, 21, and 28 days aged at 37℃ in a various organic and solutions(such as acetic acid, lactic acid and citric acid) and deionized water, surface roughness and color coefficients measuements were carried out. Then, surface roughness changes(ΔRa) and (ΔE) of specimens were calcuiated. From the experiment, the following results were obtained : 1. Chemical-cured glass ionomer cements showed statistically surface roughness changes after aged in the deionized water(P<0.10), and light-cured glass ionomer cements showed statistically significant color changes after aged in the deionized water(P<0.10). 2. Chemical-cured glass ionomer cements showed statistically color changes after aged in acetic acid solutions (P<0.10), and light-cured glass ionomer cements did not showed statistically significant surface roughness changes, but showed statisticall significant color changes after aged in acetic acid solutions (P<0.10). 3. Chemical-cured glass ionomer cements did not showed statistically significant color changes as a function of concentration of lactic acid solutions (p>0.10), and light-cured glass ionomer cements showed statistically significant color changes after aged in acetic acid solutions. 4. Chemical-cured glass ionomer cements did not showed statistically significant surface roughness changes as a function of concentration iof lactic acid solutions (p>0.10), but showed significantly less solubility in lactic acid solutions than chemical-cured glass ionomer cements.

유전자 검색을 위한 DNA 칩 제작용 microarrayer의 개발

이현동,김기대,김찬수,임용표,박정규 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.1

외국의 경우 게놈 연구 및 바이오 산업에 DNA 칩을 제작할 수 있는 로봇 시스템을 싼 가격에 사용하고 있으나 우리나라의 경우 자동화 시스템을 비싼 가격에 외국에서 도입하여 사용하기 때문에 바이오 산업 및 연구 분야에서의 생산비를 높이게 돼 국내외적으로 생명공학의 경쟁력을 저하시키는 원인이 된다. 따라서, 본 연구에서는 유전체 연구에 필수적인 DNA 칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며, 그 구체적인 연구결과는 다음과 같다. 1. 본 연구에서는 DNA칩 제작을 위한 연구용 pin 타입 microarrayer를 개발하였으며 3축 직교좌표형 로봇 본체 DNA를 묻혀 silylated 슬라이드에 점착하는 DNA 점착 헤드, 칩 및 웰 플레이트 고정부, 핀을 세척 및 건조하는 세척 및 건조장치 등으로 시스템을 구성하였다. 2. DNA 점착 헤드는 DNA 점착시 제도용 펜촉을 사용하도록 설계, 제작하였으며, 슬라이드에 DNA를 점착할 때는 핀이 일정한 힘으로 슬라이드를 누르며 점착할 수 있도록 자석의 반발력을 이용하였다. 3. DNA 점착 헤드 핀의 세척을 위하여 증류수 분사 및 진동 브러쉬를 이용하였으며 세척실험 결과, 핀을 1㎜/s로 이동시키며 브러쉬를 통과하도록 하는 방법이 세척효과가 높은 것으로 나타났으며, 핀 건조실험결과는 8.5㎏/㎤의 압축공기를 30초 동안 핀에 분사하였을 때 핀이 건조되는 것으로 나타났다. 4. 본 로봇 시스템을 이용하여 DNA를 12장의 슬라이드에 모두 점착시키기 위하여 웰 플레이트에서 핀이 DNA를 묻히는 실험을 실시한 결과, 10초 이상 핀에 DNA를 묻혔을 때 슬라이드 12장을 모두 찍는 것으로 나타났으며, 슬라이드에 핀이 1초간 접촉할 때의 DNA 스팟의 크기는 평균 280㎛가 되는 것으로 나타났다. 최소 점 간격을 0.32㎜로 설정한 후 DNA를 점착해 본 결과 최대 8,100여 점의 DNA 스팟을 한 슬라이드에 점착할 수 있는 것으로 나타났다. 5. 본 로봇 시스템은 12장의 동일 DNA 칩을 생성하기 위해 핀의 세척, 건조, DNA를 묻히는 과정 및 DNA 점착 등의 한 과정을 2분 50초 동안 수행할 수 있는 것으로 나타났다. This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28㎜ on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32㎜, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

아몰퍼스 자성박대의 자기센서 응용에 관한 연구

金大柱,林在根,金玄郁,愼鏞鎭 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

In this thesis, we have described about application of amorphous magnetic ribbon of FeCoSiB compound to magnetic sensing device. As the samples of our experiment, we used a near zero-magnetostrictive amorphous magnetic ribbon of (Fe0.06Co0.94)79Si2.1B18.9 composition with its thickness of 26[㎛], its length of 5[㎝] and its width of 2[㎜], which was fabricated by rapidly quenching method. In order to improve the magnetic properties of the samples, we annealed them under magnetic field where the range of the crystallization temperature Tx and the Curie temperature Tc were 300~400[℃]. Then, by measuring them with X-ray diffraction(XRD) and vibrating sample megnetometer(VSM) analysis method. we found Tx≒451[℃] and Tc≒441[℃]. From the hysteresis curve of the finally annealed sample ribbon, we found the Large Bakhausen Jump(LBJ) effect at the reversal magnetization field Hw≒0.5[Oe]. This LBJ effect was also observed at the variation of the magnetic domain in the process of magnetization. Thus, it is proved that the amorphous magnetic ribbon of FeCoSiB compound can be use as a magnetic sensing device.