PCR을 이용한 국내 및 수입 축산물 유래 Listeria monocytogenes의 유전학적 분석연구

우용구,이수화,이철현,최정수,류재두,김영일,이오수,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

One hundred-fifty two of Listeria strains were isolated from domestic chicken carcasses, a slaughter-houses from nationwide from 1996 to 1997 and imported livestock products from a ten foreign countries including the USA, China, France and Thailand. To substitute the laborious and time consuming procedures of clinical laboratory standard diagnostic methods, the rapid and specific multiplex PCR(M-PCR), which was designed to amplify a three kinds of genes simultaneously f3r specific detection and differentiation of L. monocytogenes(LM) by one step procedure, was established. The LM strains confirmed with M-PCR were analysed to their genetic diversities by RAPD using the two primers(D87 & MMTl), and also evaluated on their discriminatory abilities(na) between two primers. our study also conducted the REP-PCR and ERIC-PCR for evaluation of the possibility of REP and ERIC elements for genetic subtyping of LM strains. According to the REP-PCR and ERIC-PCR fingerprinting patterns, LM strains were divided into 7(ERIC-type) and 4(REP-type) major clusters at the relative clone cut off value of 80%. According to the computer analysis of PCR results using the GelCompar Ⅱ software, ERIC-PCR(DI=0.955) had expressed the most high discriminatory ability and followed by REP-PCR(DI=0.952), RAPD(D87; 0.954, MMT1; 0.937), in order. The combined analysis of the present PCR results was also expressed the reliable and useful discriminatory ability(DI=0.916). This study suggested that PCR based methods could be used as an reliable, reproducible, rapid, and highly discriminatory method for the genetic differentiation of genus Listeria.

내동정맥루술후의 수지운동이 동정맥루의 혈류와 개존에 미치는 영향

신현석,조원현,이성문,우성구,김현철 大韓脈管外科學會 1995 大韓脈管外科學會誌 Vol.11 No.1

Pendulum 장치의 상악대구치의 원심이동에 대한 효과

이창섭,김재광,강덕일,송광철,정현구,이상호 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

Ⅱ급 치성교합을 가진 환자에서는 I급 구치부 교합관계를 형성하기 위하여 상악 대구치의 원심이동이 필요하다 본 연구에서는 pendulum장치를 이용한 상악 대구치의 원심이동시 대구치의 이동양상과 고정원의 전방이동 및 정출정도 등 치아와 골격에 미치는 영향을 평가하기 위하여 시행되었다. 상악 제1대구치를 원심이동시켜 1급 구치관계가 형성된 19명의 환자 (남: 8명. 여: 11명, 평균연령 11.68±1.52세)에서 장치 장착 전과 장치제거 직후에 얻어진 측면두부방사선사진을 비교 ·분석하여 다음과 같은 결과를 얻었다. 1.I급 교합관계를 형성하기 위해서 제 1대구치는 평균 2.94± 1.54mm 원심이동하였으며 . 1.17±0.97mm 함입되었고, 소요기간은 평균 18.13±7.95일이었다. 2.고정 원으로 사용된 치아는 1.34± 1.40mm로 유의하게 전방 이동하였으며, 0.48±0.99mm 정출되었다. 또한 절치는 유의한 전방이동 및 순측경사가 발생하였다(P<0.05) . 3.구개평면과 하악평면이 이루는 각은 유의한 증가를 보였다(P(0.05). 4.제1소구치 또는 제1유구치가 고정 원으로 이용된 경우 제2소구치 또는 제2유구치가 이용된 경우 보다 전방이동이 증가하였다(P<0.005). 5. 제2대구치의 발육단계에 따른 제1대구치 및 제2대구치의 이동양상은 어떤 항목과도 유의성이 없었다(P<0.05). 6.얻어진 총 공간 중 74%는 제1대구치의 원심이동이,26%는 소구치의 전방이동에 의하여 얻어졌다. 이상의 결과를 종합하여 볼 때 pendulum appliance는 상악 대구치의 원심이동에 매우 효과적이었으며. 고정원의 소실을 최소화하기 위해 제2유구치 또는 제 2소구치가 포함되어야 한다. 또한 제1대구치의 원심경사는 필연적으로 일어나며. 이를 최소화하기 위한 노력이 계속되어야 할 것으로 사료된다. Treatment of class Ⅱ malocclusions require distalization of maxillary molars into class I relationship. Intra-arch distal molar movement techniques have recently assumed an important role in young patients. In this study, the dental and skeletal effects of the pendulum appliance were evaluated by means of cephalometric radi-ographs. The samples were consisted of 19 patients: 11 females and 8 males, mean age 11.68± 1.52 years. Measurements were obtained from cephalometric prior to and the day of removal of the pendulum appliance. Treatment changes were analyzed. The following results were obtain : 1. The pendulum appliance produced 2.94±1.54mm distal molar movement with a mean intrusion of 1 17± 0.97mm, mean period 18.13±7.95 weeks. 2. The anchor tooth was 1.34± 1.40mm forward movement and 0.48±0.99mm extrusion, and labial tilting of incisors. 3. The angle between palatal plane and mandibular plane increased significantly 4 There was no significant difference in according to 2nd molar position. 5. Total movement was consisted of 74% distal movement of 1st molar and 26% forward movement of the an-chor tooth

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.

저장온도 및 Blanching 처리가 유자의 저장성에 미치는 영향

김현구(Hyun-Ku Kim),박무현(Moo-Hyun Park),이영철(Young-Chul Lee),김홍만(Heung-Man Kim),장학길(Hak-Gil Chang) 한국식품과학회 1995 한국식품과학회지 Vol.27 No.3

호흡기질환 예방을 위한 군 생활관 실내공기질 조사

전철구 ( Jun Chul Ku ),정희생 ( Jung Hee Sang ),김명철 ( Kim Myung Chul ),심동현 ( Shim Dong Hyun ),문화영 ( Moon Hwa Young ),이종태 ( Lee Jong Tae ),서재기 ( Seo Jae Gi ),정철희 ( Jung Chul Hee ) 국군의무사령부 2010 대한군진의학학술지 Vol.41 No.1

Objective: The purpose of this study is to have the basis data for indoor air quality at barracks to prevent soldier's respiratory disease in military Method: We measured the indoor air quality of barracks in concentration with five factors such as airborne microbes, carbon dioxide, fine dust, temperatures and relative humidity at every month from June to August in 2009. We measured the indoor air quality at various time and at the three buildings that were classified year built. Results: The concentration shows significant difference by the inspected time not by the year built of the buildings. The concentration of airborne microbes, carbon dioxide were highest at 11:00 pm (p<0.01), and those of fine dust were highest at night(p<0.01). Temperatures were highest at 3:00 pm (p<0.01), and relative humidity were highest at 9:00 pm (p<0.05). Conclusion: Government rules should be legitimated for engineered ventilation system and periodical estimation of indoor air quality at barracks to prevent soldier's respiratory disease.

POSTERS / PM-059 : CHARACTERIZATION OF FERRIC LEGHEMOGLOBIN REDUCTASE IN SOYBEAN NODULES

(Hyun Mi Kim),(Chul Hwan Kim),(Kyung Ku Ahn) 한국생화학분자생물학회 (구 한국생화학회) 1999 6th IUBMB SEOUL CONFERENCE Vol.- No.-

A Disease Model Pig Over-Expressing Mouse PEA15 Showed Symptoms of Diabetes Mellitus

Hyun-Mi Kim,Hyun-Min Kim,Hak-Jae Chung,Byong-Chul Yang,Kyung-Woon Kim,Jin-Ki Park,Sung-Ku Hong,Hee-Kyung Chung,Won-Kyong Chang,Hwi-Cheul Lee,Deug-Woo Han 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

Phosphoprotein Enriched in Astrocytes (PEA15) is a 15kD-sized intracellular signaling protein, highly expressed in astrocytes and constitutively expressed in peripheral tissues. Recently it was found that PEA15 expression was elevated in patients suffering type 2 diabetes and suggested to be involved in the syndrome of insulin resistance. To investigate the functional role of PEA15 for the control of blood glucose level, we produced a transgenic pig over-expressing mouse PEA15 (mPEA15). As a model animal, pig has many advantages. They have a higher fecundity and a short generation time and are physiologically similar to human. Using the transgenic pig, we carried out a series of experiments to establish a link between PEA15 expression and the insulin resistance. Our results suggested that, compared with control pig, mPEA15 pig has, (1) a higher blood resistin level, (2) a lower cell membrane-embeded GLUT4 level, and (3) a lower glucose clearing ability based on oral glucose tolerance test (OGTT). When our results combined, it can be concluded that mPEA15 over-expressing pig has many symptoms of insulin resistance and these pigs will become a useful disease model to investigate diabetes mellitus in the near future.

A Disease Model Pig Over-Expressing Mouse PEA15 Showed Symptoms of Diabetes Mellitus

Hyun-Mi Kim,Hyun-Min Kim,Hak-Jae Chung,Byong-Chul Yang,Kyung-Woon Kim,Jin-Ki Park,Sung-Ku Hong,Hee-Kyung Chung,Won-Kyong Chang,Hwi-Cheul Lee,Deug-Woo Han 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2

고용량 항암요법후 말초혈액 자가조혈모세포 이식환자에서의 감염 양상

구성현,최소연,조요한,한동석,마경애,김현수,김효철 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

목적: 동종골수이식이나 자가골수이식, 말초혈액 자가조혈모세포이식에서 공통적으로 이병률과 사망률에 관여하는 중요한 인자중의 하나가 항암 치료후 발생하는 감염이다. 감염의 발생빈도 및 정도에 대해서 확실히 알수 있고, 위험인자를 감별해 낼수 있다면 고용량 항암요법 후에 발생하는 이병률과 사망률을 줄여 더 나은 결과를 얻을수 있다고 사료되어 저자 등은 고용량 항암요법을 시행한 환자들을 대상으로 후향적인 연구를 통해 감염 및 그로 인한 합병증을 발생빈도, 정도 및 감염과 관련된 위험인자를 규명하려고 하였다. 방법: 1995년 11월부터 96년 8월까지 아주대학교병원에서 고용량 항암요법후에 말초혈액 자가조혈모 세포이식을 시행한 33명의 환자를 대상으로 하여 나이, 성별, 진단명, 조혈모세포이식 날짜, 사용한 항암제의 종류, 방사선 치료 유무 여부, 호중구 감소증 기간, 사망여부, 사망원인을 분석하였다. 결과 33명의환자에서 37회(4명은 2번 항암치료)의 고용량 항암 요법을 시행한 결과를 분석하였다. 1) 기존에 가지고 잇는 질환을 종류별로 보면, non-Hodgkin's lymphoma(n=13), Hodgkin's disease(n=1), breast cancer(n=5), stomach cancer(n=14)이었으며 모든 환자가 기존의 항암 치료에 불응하는 악성 종양이었다. 2) 대상환자의 평균 연령은 42세이었으며 여자가 18명이었고, 평균 호중구 감소기간은 9.62일 이었다. 3) 37회 고용량 항암 요법후 8회를 제외한 29회 (78.3%)에서 열이 있었으며, 29회의 발열 중 8예(21.6%)에서 감염이 균 검출로 증명되었거나 임상적으로 확실히 의심되었다. 8회의 감염중 Staphylococcus epidermidis가 1예, E coli가 2예, Bacteroides fragilis가 1예 있었다. 4) 33명의 환자중 모두 10명이 현재까지 추적관찰중 사망하였는데 이중 2명이 고용량 항암요법후 호중구가 감소되어있는 기간에 발생한 패혈증으로 사망하여 사망원인이 고용량 항암요법에 의한 합병증과 직접적인 연관이 있었다. 5) 감염에 연관되는 위험인자를 알아보기 위하여 나이, 성별, 방사선치료유부, 호중구 감소증 기간, 악성종양 종류 등을 감염군과 비감염군으로 나누어 비교하였는데 모든 항목에서 감염군과 비감염군 사이에 통계적으로 의미있는 차이를 찾을수 없었다. 결론: 고용량 항암요법후 감염이 가장 중요한 이병률 및 사망률의 원인이 되고 있는데 현재 말초혈액 자가조혈모 세포이식후 조혈촉진인자를 사용하여 감염 발생률을 많이 줄이기는 하였으나 앞으로 좀더 많은 환자를 오랜기간 추적 관찰하여 감염으로 인한 문제점을 찾아 개선해 나가야 하겠다. Objectives: Infection is one of the most important factor in the morbidity and mortality of both allogenic and autologous bone marrow transplantation, and peripheral blood stem cell transplant patients. Therefore, an accurate knowledge of the incidence, severity, and risk factors can lead to reduction in the morbidity and mortality. We performed a retrospective analysis in patients with high dose chemotherapy to clarify these infections and also the incidence of complication, severity and associated risk factors. Methods: The subjects of our study were patients who had been admitted at Ajou University Hospital from November 1995 to August 1996 and received autologous peripheral blood stem cell transplantation(PBSCT) after high dose chemotherapy. There were 33 patients in all and the following were analyzed : age, sex, disease diagnosis, date of PBSCT, the duration of neutropenia, and the cause of death. Results: The results of 37 trials (4 patients had two trials) of high dose chemotherapy in 33patients were analyzed. ① The diagnosis included non-Hodgkin's lymphoma(n=13), Hodgkin's disease(n=1), breast cancer(n=5), stomach cancer(n=14). All of the patients were refractory to the conventional chemotherapy. ② The average age was 42years, 18 of the patients were female and the mean duration of netropenia was 9.62days. ③ 29 out of37trials was associated with fever. In 8 out 29case, infection was diagnosed by detection of bacteria or clinically suspicious. Staphylococcus aureus was detected in 4case, Staphylococcus epidermidis in 1 case, E coli in 2 case, and Bacteroides fragilis 1 case. ④10patients died during follow up. 2 case died of sepsis during the period of neurtropenia. Therefore 2 death were directly related to the complications of high dose chemotherapy. ⑤The following factors were analyzed in both infected and non-infected group to determine if there any significant different: age, sex, radiation therapy, duration of neutropenia, disease. There was no significant different between the 2 groups. Conclusion: Infection is the most important cause of morbidity and mortality in high dose chemotherapy. The rate of infection has been largely reduced as a result of rapid marrow engraftment following autologous PBSCT. A longer duration of follow up must be done on more patients to find and improve the problems after autologous PBSCT.