상사코칭 구성개념의 상대적 영향력

양병철(Yang Byong Chul),김병우(Kim Byong Woo),김정훈(Kim Jung Hoon) 제주대학교 관광과경영경제연구소 2016 産經論集 Vol.36 No.-

Changes in the Catalytic Properties of Pyrococcus furiosus Thermostable Amylase by Mutagenesis of the Substrate Binding Sites

Yang, Sung-Jae,Min, Byoung-Chul,Kim, Young-Wan,Jang, Sang-Mok,Lee, Byong-Hoon,Park, Kwan-Hwa American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.17

<B>ABSTRACT</B><P><I>Pyrococcus furiosus</I> thermostable amylase (TA) is a cyclodextrin (CD)-degrading enzyme with a high preference for CDs over maltooligosaccharides. In this study, we investigated the roles of four residues (His414, Gly415, Met439, and Asp440) in the function of <I>P. furiosus</I> TA by using site-directed mutagenesis and kinetic analysis. A variant form of <I>P. furiosus</I> TA containing two mutations (H414N and G415E) exhibited strongly enhanced α-(1,4)-transglycosylation activity, resulting in the production of a series of maltooligosaccharides that were longer than the initial substrates. In contrast, the variant enzymes with single mutations (H414N or G415E) showed a substrate preference similar to that of the wild-type enzyme. Other mutations (M439W and D440H) reversed the substrate preference of <I>P. furiosus</I> TA from CDs to maltooligosaccharides. Relative substrate preferences for maltoheptaose over β-CD, calculated by comparing <I>k</I>cat/<I>Km</I> ratios, of 1, 8, and 26 for wild-type <I>P. furiosus</I> TA, <I>P. furiosus</I> TA with D440H, and <I>P. furiosus</I> TA with M439W and D440H, respectively, were found. Our results suggest that His414, Gly415, Met439, and Asp440 play important roles in substrate recognition and transglycosylation. Therefore, this study provides information useful in engineering glycoside hydrolase family 13 enzymes.</P>

Isoprenyl carboxyl methyltransferase inhibitors: a brief review including recent patents

Yang, Woo Seok,Yeo, Seung-Gu,Yang, Sungjae,Kim, Kyung-Hee,Yoo, Byong Chul,Cho, Jae Youl Springer Vienna 2017 Amino acids Vol.49 No.9

<P>Among the enzymes involved in the post-translational modification of Ras, isoprenyl carboxyl methyltransferase (ICMT) has been explored by a number of researchers as a significant enzyme controlling the activation of Ras. Indeed, inhibition of ICMT exhibited promising anti-cancer activity against various cancer cell lines. This paper reviews patents and research articles published between 2009 and 2016 that reported inhibitors of ICMT as potential chemotherapeutic agents targeting Ras-induced growth factor signaling. Since ICMT inhibitors can modulate Ras signaling pathway, it might be possible to develop a new class of anti-cancer drugs targeting Ras-related cancers. Researchers have discovered indole-based small-molecular ICMT inhibitors through high-throughput screening. Researchers at Duke University identified a prototypical inhibitor, cysmethynil. At Singapore University, Ramanujulu and his colleagues patented more potent compounds by optimizing cysmethynil. In addition, Rodriguez and Stevenson at Universidad Complutense De Madrid and Cancer Therapeutics CRC PTY Ltd., respectively, have developed inhibitors based on formulas other than the indole base. However, further optimization of chemicals targeted to functional groups is needed to improve the characteristics of ICMT inhibitors related to their application as drugs, such as solubility, effectiveness, and safety, to facilitate clinical use.</P>

<i>Momordica charantia</i> Inhibits Inflammatory Responses in Murine Macrophages via Suppression of TAK1

Yang, Woo Seok,Yang, Eunju,Kim, Min-Jeong,Jeong, Deok,Yoon, Deok Hyo,Sung, Gi-Ho,Lee, Seungihm,Yoo, Byong Chul,Yeo, Seung-Gu,Cho, Jae Youl World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.2

<P><I>Momordica charantia</I> known as bitter melon is a representative medicinal plant reported to exhibit numerous pharmacological activities such as antibacterial, antidiabetic, anti-inflammatory, anti-oxidant, antitumor, and hypoglycemic actions. Although this plant has high ethnopharmacological value for treating inflammatory diseases, the molecular mechanisms by which it inhibits the inflammatory response are not fully understood. In this study, we aim to identify the anti-inflammatory mechanism of this plant. To this end, we studied the effects of its methanol extract (Mc-ME) on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Specifically, we evaluated nitric oxide (NO) production, mRNA expression of inflammatory genes, luciferase reporter gene activity, and putative molecular targets. Mc-ME blocked NO production in a dose-dependent manner in RAW264.7 cells; importantly, no cytotoxicity was observed. Moreover, the mRNA expression levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 were decreased by Mc-ME treatment in a dose-dependent manner. Luciferase assays and nuclear lysate immunoblotting analyses strongly indicated that Mc-ME decreases the levels of p65 [a nuclear factor (NF)-<TEX>$ \kappa $</TEX>B subunit] and c-Fos [an activator protein (AP)-1 subunit]. Whole lysate immunoblotting assays, luciferase assays, and overexpression experiments suggested that transforming growth factor <TEX>$ \beta $</TEX>-activated kinase 1 (TAK1) is targeted by Mc-ME, thereby suppressing NF-<TEX>$ \kappa $</TEX>B and AP-1 activity via downregulation of extracellular signal-regulated kinases (ERKs) and AKT. These results strongly suggest that Mc-ME exerts its anti-inflammatory activity by reducing the action of TAK1, which also affects the activation of NF-<TEX>$ \kappa $</TEX>B and AP-1.</P>

Indoor LED Light Switch with Step-by-Step Illumination Reduction

Byong-Moon Yang,Hee-Chul Eun,Hong-Sub Min,Jang-Su Kang,Jae-Wook Song,Jae-Sang Cha 보안공학연구지원센터 2013 International Journal of Smart Home Vol.7 No.4

Pyrrole-Derivative of Chalcone, (E)-3-Phenyl-1-(2-Pyrrolyl)-2-Propenone, Inhibits Inflammatory Responses via Inhibition of Src, Syk, and TAK1 Kinase Activities

Yang, Sungjae,Kim, Yong,Jeong, Deok,Kim, Jun Ho,Kim, Sunggyu,Son, Young-Jin,Yoo, Byong Chul,Jeong, Eun Jeong,Kim, Tae Woong,Han Lee, In-Sook,Cho, Jae Youl The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.6

(E)-3-Phenyl-1-(2-pyrrolyl)-2-propenone (PPP) is a pyrrole derivative of chalcone, in which the B-ring of chalcone linked to ${\beta}$-carbon is replaced by pyrrole group. While pyrrole has been studied for possible Src inhibition activity, chalcone, especially the substituents on the B-ring, has shown pharmaceutical, anti-inflammatory, and anti-oxidant properties via inhibition of NF-${\kappa}B$ activity. Our study is aimed to investigate whether this novel synthetic compound retains or enhances the pharmaceutically beneficial activities from the both structures. For this purpose, inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 cells were analyzed. Nitric oxide (NO) production, inducible NO synthase (iNOS) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) mRNA expression, and the intracellular inflammatory signaling cascade were measured. Interestingly, PPP strongly inhibited NO release in a dose-dependent manner. To further investigate this anti-inflammatory activity, we identified molecular pathways by immunoblot analyses of nuclear fractions and whole cell lysates prepared from LPS-stimulated RAW264.7 cells with or without PPP pretreatment. The nuclear levels of p50, c-Jun, and c-Fos were significantly inhibited when cells were exposed to PPP. Moreover, according to the luciferase reporter gene assay after cotransfection with either TRIF or MyD88 in HEK293 cells, NF-${\kappa}B$-mediated luciferase activity dose-dependently diminished. Additionally, it was confirmed that PPP dampens the upstream signaling cascade of NF-${\kappa}B$ and AP-1 activation. Thus, PPP inhibited Syk, Src, and TAK1 activities induced by LPS or induced by overexpression of these genes. Therefore, our results suggest that PPP displays anti-inflammatory activity via inhibition of Syk, Src, and TAK1 activity, which may be developed as a novel anti-inflammatory drug.

Pyrrole-Derivative of Chalcone, (E)-3-Phenyl-1-(2-Pyrrolyl)-2- Propenone, Inhibits Inflammatory Responses via Inhibition of Src, Syk, and TAK1 Kinase Activities

( Sungjae Yang ),( Yong Kim ),( Deok Jeong ),( Jun Ho Kim ),( Sunggyu Kim ),( Young-jin Son ),( Byong Chul Yoo ),( Eun Jeong Jeong ),( Tae Woong Kim ),( In-sook Han Lee ),( Jae Youl Cho ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.6

(E)-3-Phenyl-1-(2-pyrrolyl)-2-propenone (PPP) is a pyrrole derivative of chalcone, in which the B-ring of chalcone linked to bcarbon is replaced by pyrrole group. While pyrrole has been studied for possible Src inhibition activity, chalcone, especially the substituents on the B-ring, has shown pharmaceutical, anti-inflammatory, and anti-oxidant properties via inhibition of NF-kB activity. Our study is aimed to investigate whether this novel synthetic compound retains or enhances the pharmaceutically beneficial activities from the both structures. For this purpose, inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 cells were analyzed. Nitric oxide (NO) production, inducible NO synthase (iNOS) and tumor necrosis factor-a (TNF-a) mRNA expression, and the intracellular inflammatory signaling cascade were measured. Interestingly, PPP strongly inhibited NO release in a dose-dependent manner. To further investigate this anti-inflammatory activity, we identified molecular pathways by immunoblot analyses of nuclear fractions and whole cell lysates prepared from LPS-stimulated RAW264.7 cells with or without PPP pretreatment. The nuclear levels of p50, c-Jun, and c-Fos were significantly inhibited when cells were exposed to PPP. Moreover, according to the luciferase reporter gene assay after cotransfection with either TRIF or MyD88 in HEK293 cells, NF-kB-mediated luciferase activity dose-dependently diminished. Additionally, it was confirmed that PPP dampens the upstream signaling cascade of NF-kB and AP-1 activation. Thus, PPP inhibited Syk, Src, and TAK1 activities induced by LPS or induced by overexpression of these genes. Therefore, our results suggest that PPP displays anti-inflammatory activity via inhibition of Syk, Src, and TAK1 activity, which may be developed as a novel anti-inflammatory drug.

결핵균의 중식 및 단백질함량과 Tuberculin의 생물학적 효능간의 상관관계

최철순,곽병은,양용태,정상인 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.2

In the epidemiological survey of tuberculosis with tuberculin antigen, there are two problems. The one is a lack sensitivity or occurrence of false negative reaction and the other is a low specificity or the development of false positive reaction. During the last three decades, the general objective of research works on tuberculin preparations was to obtain a more purified tuberculin that provides a higher sensitivity and specificity. However, there have been little emphasis on the selection of media which yield the maximum quantities of active tuberculoprotein. The purpose of present work was to compare the amount of the bacterial growth, yield of tuberculoprotein and biological potency of tuberculins prepared from four different synthetic media. In addition, sensitivity and specificity of heart-concentrated synthetic medium(HCSM), of purified protein dervative (PPD) and of Seibert's Fraction A(SFA) were tested in guinea pigs sensitized with M. tuberculosis-bovis complex and atypical mycobacteria. The results are summarized as follows: 1. The largest amount of bacterial growth of M. bovis occurred in Dorset-Henley's medium at 7 weeks cultivation and it was 1.7 times or above compared with those of Lind's medium, Long-Seibert's medium and Sauton's medium. 2. The yield of tuberculoprotein in the culture medium was the highest in Lind's medium 13 weeks cultivation and was 1.5 times as much compared with that of Dorset Henley's medium, 1.7 times of Long-Seibert's medium and 7.4 times of Sauton's medium, respectively. 3. The highest biological titre, 1:400, of tuberculin appeared in the HCSM tuberculin derived from either Sauton's medium or Long-Seibert's medium. The biological titres of tuberculins prepared from Dorset-Henley's medium and Long-Seibert's medium were 1:300 and 1:200, respectively. 4. With the HCSM tuberculin, there observed little differences in the sensitivity and specificity between PPD tuberculin and SFA tuberculin, but high degree of cross reactions were elicited with HCSM tuberculin. These results indicated that there could exist none of simple relationships between bacterial growth, tuberculin yield and biological potency of tuberculin, and therefore, culture media should be carefully selected for production of tuberculin.

점등스위치를 이용한 LED 조명 조도제어 장치

양병문(Byong-Moon Yang),은희철(Hee-Chul Eun),민홍섭(Hong-Sub Min),강장수(Jang-Su Kang),송재욱(Jae-Wook Song) 한국조명·전기설비학회 2013 한국조명·전기설비학회 학술대회논문집 Vol.2013 No.5

Enhancement of Antigen-specific Antibody and CD8⁺ T Cell Responses by Codelivery of IL-12-encapsulated Microspheres in Protein and Peptide Vaccination

Sung, Young-Chul,Lee, Sung-Hee,Kim, Byong-Moon,Kwak, Hyun-Hee,Kim, Hye-Ju,Yang, Se-Hwan,Chang, Jun,Park, Su-Hyung 대한면역학회 2007 Immune Network Vol.7 No.4

Although IL-12 has been widely accepted to playa central role in the control of pathogen infection, the use of recombinant IL-12 (rIL-12) as a vaccine adjuvant has been known to be ineffective because of its rapid clearance in the body. Methods: To investigate the effect of sustained release of IL-12 in vivo in the peptide and protein vaccination models, rIL-12 was encapsulated into poly (A_DL-lactic-co-glycolic acid) (PLGA). Results: We found that codelivery of IL-12-encapsulated microspheres (IL-12EM) could dramatically increase not only antibody responses, but also antigen-specific CD4⁺ and CD8⁺ T cell responses. Enhanced immune responses were shown to be correlated with protective immunity against influenza and respiratory syncytial virus (RSV) virus challenge. Interestingly, the enhancement of CD8⁺ T cell response was not detectable when CD4⁺ T cell knockout mice were subjected to vaccination, indicating that the enhancement of the CD8⁺ T cell response by IL-12EM is dependent on CD4⁺ T cell help. Conclusion: Thus, IL-12EM could be applied as an adjuvant of protein and peptide vaccines to enhance protective immunity against virus infection.