Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

RO-TiO_2-P_2O_5계 다공질 글라스 세라믹스의 제조

강원호,윤영진,이용수 단국대학교 2000 論文集 Vol.35 No.-

Porous glass ceramics composed of 1Ag_2O·4Li_2O·2OTiO_2·27P_2O_5·36CaO with 0.05-1.5mole CuO were prepared by melting and 2 step heat treatment for nucleation at 620℃ for 24 hours and crystallization at 730℃ for 24 hours. Crystal phases were AgYi_2(PO_4)_3, LiTi_2(PO_4)_3 and β-Ca_3(PO_4)_2. β-Ca_3(PO_4)_2 crystal phase was selectively leached out in IN-HCL solution for 3 days, leaving AgTi_2(PO_4)_3 and LiTi_2(PO_4)_3 crystal phases.

올인원 접착제의 미세누출에 관한 정량적 분석

강용희,신수일,신동훈 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.1

All-in-one adhesives were recently developed for reducing the techique sensitivity and chair time, but lots of concerns were made on bondability, longevity, and microleakage. The object of this study was to evaluate microleakage and marginal quality of all-in-one adhesives using electrochemical method and SEM analysis quantitatively. After making Class V cavities, they were bulk filled with Heliomolar(#A1) after surface treatment with three adhesives: Adper Prompt (Group A), One up Bond F (Group O), Xeno Ⅲ(Group 'X), Electrical con-ductivity(microamphere, ㎂) was checked two times: before and after cavity filling. Percentage of leaky margin was estimated from SEM image (× 1,000). The data were statistically analysed: ANOVA and Paired T test for electrical conductivity, Kruskal-Wallis and Mann-Whitney test for marginal quality, Spearman's rho test for checking of relationship between 2 methods the result were as follows: 1. There was no difference in microleakage between adhesive systems and every specimen showed some of microleakage after filling. 2. Microleakage was reduced about 70% with composite resin filling. 3. Marginal quality was the best in group A, decreasing among groups in the following order: group O, followed by group X. There were significant differences between group A and group X(p=0.015), and between group O and group X(p=0.019) 4. There was no relationship between the microleakage measured by electrochemical method and margin-al quality measured by SEM analysis. Within the results of this study, there was no difference in microleakage groups by electrical conductivity. However, significant difference in margin quality was seen among groups. It was believed teat these dissimilar results might be induced because of their own characteristics. Analysis of microleakage needs various methods for accuracy.

퇴행성슬관절염 환자에 있어서 관절경수술의 효과

강수용,최호림,정영복 대한슬관절학회 1991 대한슬관절학회지 Vol.3 No.2

Recently arthroscopic examination and operation have replaced open arthrotomy surgery and become one of good treatment modahties in osteoarthritis of the knee which does not respond to conservative treatment. Arthroscopic debridement was carried out in 52 knees of 48 patients who had a primary diagnosis of osteoarthritis at Chung-Ang University Yong-San Hospital from January 1988 to September 1990. The patients ranged in age from 34 to 83 years with mean age of 54.9 years (male: female= 1: 1.8) and the average follow up period was 16.5 months from 12 to 36 months. Following results were obtained; 1. Arthroscopic lavage, meniscectomy, synovectomy, cartilage drilling and loose body removal were performed with overall results of 3 excellent (6%), 28 good (54%), 14 fair (27 %) and 7 fail (13 %). 2. The result of gynovectomy was most satisfactory for 2 excellent, 7 good and 2 fair. In conclusion, arthroscopic debridement can be one of the good alternative treatment methods of osteoarthritis and a time saving procedure before arthroplastic surgery of the knee.

FAC-Biofilm과 F-A 시스템에 의한 下水處理 特性

姜龍太,柳在益,玄吉秀 동아대학교 환경문제연구소 1991 硏究報告 Vol.14 No.1

Through the comparative experiment of FAC-Biofilm and F-A system using the wastewater treated by FAC process, the following conclusions ca be drawn form the results of this research. 1. Despite the fluctuation of influent concentrations, FAC process showed that since the wastewater qualities treated by it were within the range of effluent quality regulation, it was adequate as pretreatment process 2. The results of comparative experiments of FAC-Biofilm and F-A system presented that for COD and SS removal, all of both system resulted in a desirable removal rate, but NH_(4)-N was largly reduced by Biofilm process. Particulary, phosphorus removal was more effective to pass through the biofilm process after coagulation and sedimentation. 3. The waste sludge amount in FAC process was calculated by the equation of that of Contact-Stabilization Method, because MLSS concentration in reaeration basin keep being constant could be anticipated steady-state in operation the FAC process.

Large Volume Sample Stacking 방법과 Head Column Stacking 을 이용한 프로틴과 펩타이드의 시료 농축

강대천,도길명,김용성,정두수 경남대학교 기초과학연구소 2001 硏究論文集 Vol.15 No.-

현재 세포 성분 내에서의 단백질들의 기능규명, 새로운 신약의 개발 등은 매우 중요함을 차지하고 있다. 그러나 현재의 분석 기술인 2-D 젤 모세관 전기 영동 장치, 액체 크로마토그래피 그리고 질량 분석기 등의 여러 가지 방법들은 세포 내에서 극 미량의 단백질들을 분석하는데 있어서 충분한 감도를 제공하지 못한다. 이 실험에서는 모세관 전기 영동 장치를 이용한 단백질과 펩타이드의 분석에서 시료의 낮은 농도의 감도를 향상시키기 위하여 시료 농축 방법인 Large Volume Sample Stacking 방법과 Head column stacking 방법을 이용하여 분석을 하였다. α - lactalbumin과 trypsin inhibitor들과 같은 단백질들을 위의 시료 농축 방법을 이용하여 낮은 시료의 농도 0.01 ㎍/㎖까지 농축이 가능하였다. 또한 효소로 처리한 잘려진 단백질들의 절편들도 극 미량의 low femtomole 수준까지 검출이 가능하였다. 그리고 low femtomole 수준의 단백질 절편들을 규명하기 위하여 CE-MS를 사용하였다 The identification proteins in the cell is very important for Proteomics research. However, current analytical techniques such as 2-D gel electrophoresis, 1iquid chromatography and mass spectrometry do not provide enough sensitivity for cell proteins especially for low-abundant proteins. Therefore, protein and peptide concentration method should be developed for the easy operation of current identification technologies. In order to enhance the concentration sensitivity in capillary electrophoresis of proteins deptides, large volume sample stacking using electroosmotic flow pump and LVSEP combined with Head column stacking were applied. Proteins such as α -Lactalbumin and trypsin inhibitor were successfully stacked and their limits of detection were below 0.01㎍/㎖. A large volume of digested protein samples was also stacked and the enhanced sensitivity allowed one to analyze protein digests at low femtomole levels. CE-MS was employed for the identification of protein digests at low femtomole levels.

뇌성마비 환자의 뇌자기공명영상

강용수,김봉옥,김종철,송창준 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Brain MRI findings were analyzed in 30 patients with clinical cerebral palysy to determine clinical role in assessment of time of brain damage. The patients (1 day to 14 yrs. old) underwent MR scanning and findings were correlated with clinical history retrospectively. Among 13 prematurely born patients, 11 cases showed abnormal findings in brain MR, which were periventricular leukomalacia in 10 patients and cortical gray matter disease in 1 patient. And MR scans of 17 patients born at or around the expected date of confinement were reviewed. Fifteen cases showed abnormal findings in MR, which are, cortical gary matter disease in 10 patients, deep white matter lesion in 3 patients and neuronal migration anomaly such as schizencephaly, polymicrogyria and pachygyria in 3 patients. Abnormality in contour of ventricle in prematurely born patients were dilatation of trigone & irregular ventricular contour in 4 patients and diffuse enlargement in 3patients. Among patients born at term, dilatation of trigone & irregular ventricular contour in 3 patients and diffuse enlargement in 6 patients were observed. Corpus callosal abnormalities were thinning of posterior body in 7 patients, diffuse thinning in one patient and irregularity in 4 patients among the prematurely born and thinning of posterior body in 9 patients, diffuse thinning in 2 patients, abscent in 1 patient and irregularity in 3 patients among the patients born at term. MR imaging seemed to be very useful in determining the time of the insult which could have been the cause of the cerebral palsy.

Modal SI 기법 적용을 위한 최적 측정위치 선택

강태우,권순정,김용현,신수봉 東亞大學校 建設技術硏究所 2001 硏究論文集 Vol.25 No.2

In applying a modal system identification with a limited number of measuring degrees of freedom, the selection of optimal sensor locations (OSL) is critical for a good identification. The current research proposes a new OSL method applicable to a modal system identification (SI) and compares it with a widely used OSL method. The proposed method utilizes the sensitivity of eigenvectors with respect to the structural parameters. A simulation study for a tower structure has been carried out to demonstrate the efficiency of the proposed method. Through the simulation study, the influence of OSL on the structural parameters estimated by a S1 method are investigated.

흰쥐 뇌에서 전기경련 충격에 의한 CREB 인산화의 발달단계에 따른 변화

강웅구,정희연,안용민,정선주,전송희,박주배,조수철,김용식 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적 : 이 논문에서는 뇌에서 전기경련 충격(Electroconvulsive shock. ECS)에 의한 전사인자 CREB의 인산화를 발달단계에 따라 알아봄으로써 정신장애의 신경발달학적 이해를 위한 생물학적 기반지식을 얻고자 하였다. 방 법 : 생후 7. 14. 21일 및 성년 흰쥐에 ECS를 가하고 시간별로 해마 및 소뇌 조직을 얻어 CREB의 인산화를 알아보기 위해 특이 항체로 면역블롯을 실시하였다. 결 과 : 발달단계에 따라 해마에서는 CREB이 감소한 반면 소뇌에서는 CREB이 증가하였다. 기저상태의 CREB 인산화는 해마와 소뇌에서 생후 7일에 비해 14일 이후 증가하였는데, 소뇌의 경우 CREB의 양증가와 비례하였다. ECS 후 CREB인산화 증가는 해마에서는 생후 21일 이후에 나타났으나, 소뇌에서는 생후 7일은 물론 성년에서도 나타나지 않았다. 결 론 : CREB 매개 신호전달은 발달단계별 및 조직별 활성의 차이를 나타내었으며 해마에서는 생후 21일 이후 ECS에 의해 활성화되었지만, 소뇌에서는 그렇지 않았다. 해마에서 ECS에 의한 CREB 인산화 증가는 c-fos 유전자의 발현과 관계있으리라고 생각되지만, CREB의 Ser-133 인산화 만으로는 발달단계 및 조직에 따른 c-fos 발현의 특이성을 설명할 수 없었다. Objectives : In order to understand the biological basis of neurodevelopmental perspectives of mental disorders, the authors investigated the developmental and regional changes in the phosphorylation of the transcription factor CREB following the electroconvulsive shock(ECS) in rat brain. Methods : Rats of various age groups(7, 14, 21 days postnatal and adults) were given ECS and their hippocampi and cerebella were dissected at specified time points. The content of CREB and phosphorylated CREB were measured by immunoblot analysis. Results : The amount of CREB increased in the hippocampus and decreased in the cerebellum according to the age. Baseline levels of CREB phosphorylation in both tissues were increased from postnatal 14 days, and it was proportional to the amount of CREB protein in the cerebellum. In the hippocampus, ECS increased the phosphorylation of CREB at postnatal 21 days, but in the cerebellum, ECS did not increased the phosphorylation of CREB in any age group. Conclusion : CREB mediated signal transduction pathways showed developmental and tissue-specific changes. ECS increased the phosphorylation of CREB in the hippocampus by postnatal 21 days, but not in the cerebellum. CREB activation is supposed to be related with the induction of c-fos after ECS in the hippocampus. However, the Ser-133 phosphorylation of CREB could not completely explain the developmental and tissue specificity of c-fos induction.

Pseudomonas sp.가 생성하는 cellulase의 생합성 조절

姜仁秀,鄭永哲 진주여자전문대학 1991 論文集 Vol.14 No.-

CMCase, avicelase and β-1,4-glucosidase synthesis of Pseudomonas sp, were induced by cellulosic compounds such as cellulose, xylan and CMC, and repressed by glucose The production of cellulase complex was inhibited by the addition of 0.1, 0.3, 0.6% glucose after 10 hrs incubation, indicating that cellulase complex biosynthesis is under catabolite repression conterol. CMCase, avicelase and β-1, 4-glucosidase from Pseudomonas sp. transfered to minimal medium supplied with various carbon sources were effectively induced by sophorose, and cellobiose, respectively Optimal concenrtation of sophorose and cellobiose for induction was 30㎍/㎖ and 3㎎/㎖, respectively. Cellulase induction was effective when grown in minimal medium containing inducer and 60mM phosphate at 35℃, pH 6.0.