Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

도시공원 범죄예방을 위한 CPTED 인증평가기준 정립에 관한 연구

김강일(Kang Il Kim),조준택(Joon Tag Cho),박현호(Hyeon Ho Park) 한국셉테드학회 2018 한국셉테드학회지 Vol.9 No.1

도시지역에서의 공원이란 시민들의 건강과 휴양 그리고 정서생활에 도움을 주고 녹지를 확보하여 환경에 도움을 주는 공간이다. 하지만 누구에게나 개방되어 있는 도시 공원의 특성상 공간의 안전을 위협하는 소수의 위협들은 다수의 이용자인 시민들에게 불편함을 주거나 위협을 초래하는 경우가 발생한다. 이와 관련하여 각종 선행 연구들은 공원에서 범죄가 빈번하게 발생하고, 시민들의 휴식공간이라는 공원의 제 기능을 다 하지 못하고 있다는 문제를 지적하고 있다. 특히 최근 많은 관심을 받고 있는 사회적 약자를 대상으로 한 범죄에 대응하기 위한 도시공원의 설계 관리가 중요함을 일관되게 강조하고 있다. 이에 이 연구에서는 CPTED의 기본이론을 토대로 도시공원의 범죄예방과 관련된 선행 연구를 분석하여 도시공원에 적합한 CPTED 요소를 추출하고 도시공원의 방범환경 인증평가 체크리스트를 개발하였다. 이러한 체크리스트를 바탕으로 크기별, 종류별 공원 5곳을 선정하여 실제 현장 조사를 실시하고 안전요소를 분석하여 최종 체크리스트를 도출하였다. 이 체크리스트의 최종평가분야는 관리운영, 감시, 접근통제, 영역성, 활동성 지원 CPTED요소를 기반으로 하고 이에 더하여 안내표지, 방범 및 안전시설, 가점항목으로 설정하였으며 각각의 세부평가항목으로 구성하였다. 이 연구에서 개발한 체크리스트의 평가기준은 모든 공원에 대한 범죄예방 인증평가를 수행할 수 있도록 하는 범용 체크리스트로 이러한 평가기준은 다양한 규모와 형태를 가진 공원들의 특성을 고려하여 평가와 인정의 기본원칙에 의거하여 다양한 형태로 변형되어 활용할 수 있도록 하였다. 이와 관련하여 범죄위험을 예방하는데 있어 중요한 분야의 핵심 항목들에 대해서는 높게 배점하고 정성적인 평가에 의해 배점을 차등적으로 주도록 하였다. 또한 신규 공원뿐만 아니라 기존에 있던 공원들도 적극적 개선을 통해 인정을 받게 하고자 하는 취지로 만들어 전체적인 공원 안전개선을 위한 기반이 되도록 하였다. 본 체크리스트는 경찰청이 범죄예방진단팀(CPO)을 통해 시행 중인 도시공원의 범죄예방우수시설 인증사업에 활용하고 있다. Urban public parks are supposed to be the places for citizens’ rest, healing and public health. However, as public parks are usually open for 24 hours almost every day they are often exposed to criminal attacks within the realm of the space. The crime problem therefore threatens the public safety and security. Previous studies on public parks and crime safety continuously indicate that urban parks are vulnerable to crime and disorder and became the cause of fear of crime due to poor environmental design and planning in terms of CPTED. In this context the researchers have developed a checklist for accreditation/certification of urban public parks for crime prevention by analyzing the examples applying the CPTED principles in public parks and reflecting the field survey results in South Korea. In regard to the item and point, the crucial points for public parks rated highly and the grading was differentiated by qualitative evaluation. In addition, basic principles for certification of Secure/Safe Public Parks Awards were suggested, including operating a rating system to certify public parks so as to promote efforts for improvement, not awarding the certification to parks if any relevant regulations are violated, applying the evaluation items or points in accordance with the local context. The assessment checklist can be revised and used in various ways according to the regional circumstances and can contribute to the security and safety of public parks. The Police Crime Prevention Officers (CPO) are to utilize this checklist for the Secure Public Parks Certification Awards program in order to enhance the level of security for public parks in major cities in South Korea.

태안해안국립공원 지역특화자원을 활용한 탐방프로그램 개발 연구

배준호(Joon-ho Bae),남승민(Seung-min Nam),허학영(Hak-young Heo),송창룡(Chang-ryong Song),박현숙(Hyun-suk Park) 국립공원연구원 2021 국립공원연구지 Vol.12 No.1

본 연구를 위해 먼저 국내·외 해상·해안국립공원의 운영프로그램 현황에 대한 조사를 시행하였으며, 조사된 일부 사례의 경우 기후 및 지형적 요인, 그에 따른 생태환경의 차이, 그리고 관련 법령 차이 등의 이유로 즉시 국립공원에 도입하기 보다는 중·장기적 운영계획과 인프라의 확충, 전문성 확보 등 체계적인 준비가 필요한 것으로 나타났다. 보다 적합한 탐방프로그램의 개발을 위해 사업대상지 현장방문을 실시하였으며, 기존 운영프로그램 현황, 각종 시설, 인력현황 파악, 주변 환경 분석, 담당자 인터뷰 등을 통해 맞춤형 탐방프로그램을 구성하였다. 태안해안국립공원의 경우, 연구원 협업 철새 가락지 부착체험, 태안 해변길 - 솔모랫길 탐방, 태안해변 드론 탐방, 마음의 의자, 연날리기, 새와 예술 그리고 … 등의 체험형 프로그램과 한서대학교와 함께하는 해양 안전 교육 프로그램, 유류 오염 교육 프로그램 등의 교육형 프로그램 등 모두 8가지 해양국립공원 운영프로그램이 도출되었다. 제안된 탐방프로그램을 운영하기 위해서는 별도의 장소 및 운영시설의 확충, 예산편성, 운영을 위한 타당성 검토 등이 우선되어야 할 것이다. 특히 탐방프로그램을 효율적으로 운영하기 위해서는 프로그램 지도자의 역량개발이 필요하며, 현재 운영되고 있는 프로그램을 보완하고 새로운 프로그램을 시행하기 위한 추가적 논의가 필요할 것으로 사료된다. For this study, we first examined the currently operating programs of domestic and overseas maritime and costal national parks. It was found that some of the programs require systematic preparations such as mid- and long-term operation plans, infrastructure expansion, and maintenance of professionalism rather than immediately introducing them into national parks due to the differences in climatic and geographic factors, ecological environment, and relevant regulations. To develop more suitable programs, on-site visits were made and the customized programs were suggested by closely examining the status quo and interviewing people in charge of running the currently offered programs. For Taean Coastal National Park, eight programs were recommended as feasible ones, including the experiential programs such as Bird Ringing, Taean Beach Trail - Sol Sand Trail Walking, Travel Taean Beach with Drone, Thinking Chair, Kite Flying, and Culture & Arts Program (Birds, Arts, and …), and the educational programs such as Marine Safety Training and Oil Pollution Awareness Program. For the suggested programs to be realized, the expansion of separate places and operating facilities for each program, budgeting, and feasibility study should be preceded. To operate the programs effectively and efficiently, in particular, it is necessary to develop the competency of program leaders, and additional discussion for supplement the currently operated programs and implement new programs.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Kim, Se-Jin,Ho Hur, Joon,Park, Channy,Kim, Hyung-Jin,Oh, Gi-Su,Lee, Joon No,Yoo, Su-Jin,Choe, Seong-Kyu,So, Hong-Seob,Lim, David J,Moon, Sung K,Park, Raekil Nature Publishing Group 2015 Experimental and molecular medicine Vol.47 No.2

<P>Bucillamine is used for the treatment of rheumatoid arthritis. This study investigated the protective effects of bucillamine against cisplatin-induced damage in auditory cells, the organ of Corti from postnatal rats (P2) and adult Balb/C mice. Cisplatin increases the catalytic activity of caspase-3 and caspase-8 proteases and the production of free radicals, which were significantly suppressed by pretreatment with bucillamine. Bucillamine induces the intranuclear translocation of Nrf2 and thereby increases the expression of γ-glutamylcysteine synthetase (γ-GCS) and glutathione synthetase (GSS), which further induces intracellular antioxidant glutathione (GSH), heme oxygenase 1 (HO-1) and superoxide dismutase 2 (SOD2). However, knockdown studies of HO-1 and SOD2 suggest that the protective effect of bucillamine against cisplatin is independent of the enzymatic activity of HO-1 and SOD. Furthermore, pretreatment with bucillamine protects sensory hair cells on organ of Corti explants from cisplatin-induced cytotoxicity concomitantly with inhibition of caspase-3 activation. The auditory-brainstem-evoked response of cisplatin-injected mice shows marked increases in hearing threshold shifts, which was markedly suppressed by pretreatment with bucillamine <I>in vivo</I>. Taken together, bucillamine protects sensory hair cells from cisplatin through a scavenging effect on itself, as well as the induction of intracellular GSH.</P>

점농어(Lateorabrax sp.)의 종묘생산에 관한연구 1. 稚魚期에 있어서 成長과 生存에 미치는 염분의 영향

박준택,한경호 麗水大學校 産業基術硏究所 1999 産業基術硏究所 論文集 Vol.8 No.-

점농어 稚魚(孵化後 150일째)의 염분 농도별 실험구(A : 30‰, B : 20‰, C : 10‰, D : 0‰)에서의 성장율과 생존율은 다음과 같다. 부화 150일루 稚魚들을 각각의 염분 농도에 수용하였을 때 수용 수 50일째까지는 고염분에서 성장이 다소 빨랐으나, 수용 후 60일째 부터는 담수와 저염분의 실험구에서 성장이 매우 양호하였다. 각 염분별 사육일수 (X)에 따른 전장(Y)과의 회귀직선식은 다음과 같다. 30‰ 실험구 : Y = 20.26X + 57.20 (r=0.9799), 20‰ 실험구 : Y = 19.74X + 57.82 (r=0.9825), 10‰ 실험구 : Y = 20.80X + 56.34 (r=0.9823), 담수(0‰) 실험구 : Y = 21.43X + 54.71 (r=0.9845). 염분 농도를 달리하여 사육하였을 때 生存率은 수용 초기에는 담수 실험구에서 生存率이 떨어졌으나, 수용 후 50일째부터는 오히려 담수 실험구에서 生存率이 높았으며, 수용 후 90일째부터는 生存率이 86.0∼91.0%로 실험구별로 큰 차이는 없는 것으로 나타났다. 각 실험구간의 기울기에 유의성 검정결과 담수(0‰) 실험구와 10‰, 20‰실험구 및 30‰실험구 사이에는 유의한 차이가 있었다. This study was conducted to compare the growth and survival rates of spotted sea bass, Lateorabrax sp., juveniles in different salinities (0‰, 10‰, 20‰, 30‰). The growth rate of sea bass juveniles were not observed significant difference in all test group until 50 days after rearing. After that, However rate of sea bass juveniles in low salinity was higher than the high salinity. The relationship between the days of rearing (X) and the total length (Y) of the fingerlings were as follows : 30‰ treatment group : Y = 20.26X + 57.20 (r=0.9799), 20‰ treatment group : Y = 19.74X + 57.82 (r=0.9825), 10‰ treatment group : Y = 20.80X + 56.34 (r=0.9823), 0‰ treatment group : Y = 21.43X + 54.71 (r=0.9845). The slopes of regression line estimated from 0‰, 10‰ and 20‰groups were significantly different from that of 30‰ group. Survival rate of sea bass juveniles was fall down slightly at low salinitly () until 50 days after rearing. But significant difference of survival rate was observed in all test group from 90 day after rearing to the end of experimental period.

바이러스 프로그램에 대한 백신의 치료기법에 관한 연구

박준호,채선희 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(C)

인터넷의 급속한 확산과 네트워크 상에 접속된 대량의 컴퓨터 프로그램개발 보급이 확대되면서 바이러스의 감염이 빈번하고 그 대응책인 백신에 대한 필요성이 절실하다. 본 논문에서는 여러 가지 바이러스 영역에서 메모리 상주 바이러스 종류와 특징, 동작방식 및 증상을 분석하였고 백신 프로그램이 바이러스를 치료하는 기법을 고찰하였다.

대장내시경검사에서 비특이적 염증형태로 나타난 대장선암 1예

박재현,장병익,이호찬,김성준,박준석 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.2

Colon cancer is the second most common malignancy in Korea. It is classified as superficial type, the mass type, the ulcerative type, the ulceroinfiltrative type, the diffuse infiltrative type and the unclassified type according to the colonoscopic findings. We report here on a case of colon cancer that was initially misdiagnosed as acute infectious colitis at the initial presentation. A 64-year-old man visited to Yeungnam University Hospital for watery diarrhea and lower abdominal pain. Colonoscopy revealed long segmental edematous mucosa and hyperemic mucosa with stenosis in the transverse colon. He was diagnosed as having acute infectious colitis according to the colonoscopic finding. However, two days later after colonoscopy, he visited the emergency room for hematochezia. We performed computerized tomography (CT) and obtained blood samples to find the origin of the bleeding. We found thickening of the transverse colon lumen and ascites on the CT finding and an elevated level of tumor markers; we also obtained the results of the colonoscopic biopsy that was done via colonoscopy. He was finally diagnosed as having colon cancer with carcinomatosis, a poorly differentiated adenocarcinoma.

韓國産 景天科 植物에 關한 本草學的 考察

朴柱性,新民敎,宋昊埈 圓光大學校 韓醫學硏究所 1994 원광한의학 Vol.4 No.1

For the purpose of developing Korean Herbalogy with the plants of Crassulacea which grow wild and is planted in Korea, the these and writings in hirbalogy, from literature of successive generations, have been throughly investigative and the results obtained were as follows; 1. There were totaled to 9 genera and 55 species in Korea and among them medicinal plants are 5 genera, 17species. Some 31% in total but the number of species may be added because of the similiar plants. 2. According to sum of 55 species in Crassulacea family, they were classified into Sekum genera 33, Orastachys 8, Rhodiola genera 4, Bryophyllum genera 3 etc. 3. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Crassulacea family were classified as Herba 10, Radix 4, Folium 3, Flos 1. Thus it was noticed that Herba was the main kind. 4. According to nature of medicinal plants, they were classified into cold, cool, 6: balance, 3 ; warmth, 1. Thus it was noticed that cold, cool is the main in nature of medicinal plants. 5. According to properties and principal curative action, they were classified into drugs to remove dramatic swelling 9 drugs for hemostatis 7, to remove toxic heat with febri fugal and detoxicant drugs 6. From this result, it was revealed that the plants for medical purpose in Crassulacea was 31% of the whole, in which Herba was mostly abundunt. It is considered that many experiments and clinical apporaches must be continued to use Crassulacea plants widely.

절연 파괴 특성을 이용한 에폭시 복합체의 절연 신뢰도 예측

박건호,이준웅 광운대학교 신기술연구소 1996 신기술연구소논문집 Vol.25 No.-

본 연구에서는 송배전 계통에서 변성기의 몰드 재료로서 널리 사용되고 있는 에폭시 수지를 시료로 하여 설정해 높은 배합비에 따라 5종으로 제작한 각 시편에 대해 절연 파괴 실험을 하였다. 절연파괴 데이터를 가지고 와이블 분포식을 이용하여 임의의 허용 파괴 확률에서의 허용인가 전계의 값을 추정하였으며 절연 안전성을 판단하기 위하여 경년 열화(經年劣化) 데이터의 통계 처리를 행한 결과 허용 파괴 확률을 0.1[%]로 하였을 때, 허용 인가 전계는 21.5[kV/mm] 이하가 되어야 함을 확인할 수 있었다. In this study, the dielectric breakdown of epoxy composites used for transformers was experimented and then its data were simulated by Weibull distribution probability. The more hardener increased the stronger breakdown strength at low temperature because of cross-linked density by the virtue of ester radical, and the breakdown strength of specimens with filler was lower than it of non-filler specimens because it is believed that the adding filler forms interface and charge is accumulated in it, therefore the molecular motility is raised, the electric field is concentrated, and the acceleration of electron and the growth of electron avalanche are early accomplished. From the analysis of Weibull distribution, it was confirmed that as the allowed breakdown probability was given by 0.1[%], the applied field value needed to be under 21.5[kV/mm].