유전자 지문을 이용한 축우 품종별 유전 분석

양영훈,여정수,이은준,김재우,이문연 한국축산학회 1997 한국축산학회지 Vol.39 No.6

In the analysis of genetic characteristics based on results of DNA fingerprints of Korean native cattle (Hanwoo) and other cattles(Holstein, Charolais, Aberdeen Angus, and Simmental), the Korean native cattle was found to be the lowest in homozygosity and genetic similarity within breed, allelic frequency, band frequency, and band sharing. It can be suggested that Korean native cattle have more capacity of genetic improvement than others, in the other hand strategies for genetic improvement of Korean native cattle used until now need to be reappraised.

逆流浸降 電流法에 依한 돼지의 X-, Y-精子分離 關한 硏究

李用斌,呂政秀,鄭用基,尹種擇,徐國聖 서울大學校 農科大學 1980 서울대농학연구지 Vol.5 No.2

This experiment was carried out to separate X- and Y- bearing sperm of boar semen from 4 boars raising at the College of Agriculture, Seoul National University. The boar semen collected was treated by the counter streaming sedimentation and galvanization. The results obtained were as follows; Average appearance of B-body from control was 45.2%, when the experiment was performed with counter streaming sedimentation alone appearance of B-body from top and bottom fraction were 59.4% and 36.3%, respectively. The frequency of appearance of B-body from 30(24%) and 40(19.6%) minutes among treatments were statistically significant differences. Appearance of B-body from anode was 54% and from cathode was 37.9% after counter streaming sedimentation and galvanization. The difference between anode and cathode was significant differences at the 30(14.7%), 40(19.4%) and 50(19.9%) minutes. The frequency of appearance of B-body from counter streaming galvanization was significantly higher than that from counter streaming sedimentation. Therefore, the result from counter streaming sedimentation(30 minutes) and galvanization(30 minutes) was the most useful.

DNA 분석을 통한 한우 , 연변황우 및 화우의 유전적 특성

신원집,여정수,김재우,신수길,정진우,이지홍 한국축산학회 1999 한국축산학회지 Vol.41 No.4

This study was conducted to identify the genetic parameters and genetic relationships among 3 cattle breeds of Hanwoo (Korea), Yanbian yellow cattle (China), and Wagyu (Japan). DNA fingerprinting was prepared using M13 probe and Pst 1 enzyme. Genetic homogencities of Hanwoo and Yanbian yellow cattle were comparatively lower than that of Wagyu, suggesting genetic improvement by active breeding program for Wagyu compared to Hanwoo and Yanbian yellow cattle and possibility of improvement capacity for Hanwoo and Yanbian yellow cattle. Genetic similarities between breeds were not significantly different among them, suggesting that these 3 breeds have been raised independently without any inflow or outflow of gene source for a while.

재래돼지(Korea Native Pigs) 특이 genetic marker와 육질 연관성 분석

김철욱,여정수,조광근,진상근,오명곤,박준규,권은정,홍연희,김지현,이보경,박다혜,김재우,이지홍 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6

본 연구는 재래돼지 특이적인 marker를 개발하고 이들 marker와 육질과의 연관성을 규명하기 위하여 실시하였다. 실험동물로 재래돼지와 랜드레이스 각각 30두씩 총 60두를 사용하였으며, 품종 특이 marker를 찾기 위하여 4번과 7번 염색체에 존재하는 총 60개의 microsatellite를 이용하여 PCR (polymerase chain reaction)을 수행하였다. PCR 증폭은 형광물질 Fam과 Hex, Ned로 표지된 한쌍의 microsatellite primer를 이용하였으며, 형광물질로 표식된 PCR 산물은 Genetic Analyzer ABI 310으로 전기영동하여 대립 유전자를 규명하고 통계 분석하였다. 대립 유전자에 대한 통계 분석 결과 총 60개의 microsatellite중 27개가 다양한 유전적 다형현상을 보이는 대립 유전자가 증폭되었으며 (p<0.05), 특히 SW1364와 SW445, SW1369, SWR773 microsatellite의 증폭 산물에서 재래돼지와 랜드레이스 두 품종간을 구분할 수 있는 품종 특이적인 대립 유전자를 확인하였다 (p<0.01). 또한 육질에 영향을 미치는 유전적 marker를 얻기 위해 60두 전체에 대한 육색, 지방색, 지방산 조성, 콜레스테롤 함량, 연도와 경도에 대한 육질 분석을 실시하여 육질 형질과 marker와의 연관성을 분석한 결과 육질 형질에 영향을 미치는 28개 marker를 확인하였으며, 육색과 지방색에 연관된 4개, 조직감과 연관된 10개의 marker를 확인하였다. 이렇게 선발된 marker들은 고급육 생산을 위한 돼지의 유전적 개량을 위하여 유용하게 이용될 수 있다. This study was conducted to develop Korean native pig-specific DNA markers and analyze its relativity with meat quality using 30 of Korean native pigs and Landraces, respectively. To find species-specific DNA markers, we selected and tested a total of 60 microsatellite markers on the 4 and 7 chromosome using polymerase chain reaction (PCR). PCR amplification was carried out by microsatellite primer pairs labeled with fluorescent Fam, Hex and Ned. The fluorescent labeled PCR products were electrophoresed with Genetic Analyzer ABI 310 followed by the allelic and statistical analysis. According to the allelic frequency annalysis, of the 60 microsatellite markers 27 were preduced with a variety of genetic polymorphic alleles (p<0.05). Especially, alleles of SW1364, SW445, SW1369 and SWR773 microsatellite markers were identified to be species-specific markers between two breeds (p<0.01). To analyze the meat quality we determined the meat color, fat color, fat composition, cholesterol contents and hardness, and compared these characteristics with the alleles of 36 species-specific markers in all pigs. Of the 60 microsatellite markers 28 were found to be related with meat quality; 18 for meat and fat color, 7 for fatty acid, 4 for cholesterol contents and 10 for shear force and hardness. These selected markers will be useful in the genetic improvement of pigs for an excellent meat quality.

전기영동과 산알카리처리 결합방법에 의한 돼지정액의 X 와 Y 정자 분리에 관한 연구

이용빈,서국성,여정수,정용기 ( Y . B . Lee,G . S . Suh,J . S . Yeo,Y . K . Chung ) 한국축산학회 1980 한국축산학회지 Vol.22 No.4

The combination of the acid-alkali method and eletrophoresis for X-and Y-sperm separation of boar semen was performed We used lactic acid and sodium bicarbonate in acid-alkali method, and electrophoresed at 2.5V and 200 μA. The results obtained were summarized as follows : 1. The frequency of appearance of B-body from alkali treatment of whole semen was significantly higher than that from acid treatment. 2. The frequency of appearance of B-body from alkali treatment at the anode electrode was significantly higher compared with that from acid treatment at the same electrode, while that from acid treatment at the cathode was slightly higher than that from alkali treatment at the same electrode. 3. According to time of treatment, Appearance of B-body from alkali treatment at the anode after electrophonesis was decreased, and the result from acid treatment at the same condition was the opposite, while appearance of B-body from alkali treatment at the cathode after electrophoresis was not changed and that from acid treatment at the same condition was increased. 4. Appearance of B-body from alkali treatment at the anode for 5-10minutes showed the most useful result which was higher than the result (51.9%) from electrophoresis alone by 3 %.

한국재래산양의 성장에 따른 생리적 변화와 이용 및 질병에 관한 연구

박구부,하정기,임종우,김종수,서명득,최상용,송우준,이병오,김종섭,여상건,곽수동 경상대학교 축산진흥연구소 1988 畜産振興硏究所報 Vol.15 No.1

한우의 일당 증체량에 연관된 DNA Marker 의 규명

여정수,박노형,김재우,이문연,장태경 한국축산학회 1999 한국축산학회지 Vol.41 No.4

DNA markers related to daily gain from 287 heads( 173 heads of bull and 114 heads of steer) of Hanwoo were found with distinct 3 kinds at 4.6, 3.6 and 2.8kb using DNA fingerprinting with M13/HaeIII and 2 kinds at 5.6 and 2.1kb with M13/Hinfl. It was evidently considered that 3 specific markers influenced to grow in Hanwoo, negative effect in 4.bkb and positive in o hers(3.6 and 2.8kbs) for daily gain and especially single effect at 3.bkb for bulls and combined effect of 4.6 arid 2.8 markers for steers were distinctive to daily gain with M13/HaeIII. Pedigreed pattern of specific markers with M13/HaeⅢ were observed with DNA bands at 2.8 and 3.bkb were dependent on sire. Under results of this research culling of 4.bkb marker and selection of 3.bkb marker in Hanwoo population can be expected to improve above 35㎏ at market body weight.

한국재래돼지의 경제형질에 연관된 AFLP marker 의 분석

여정수,김재우,이지홍,장태경,박영애 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.3

DNA markers related to daily gain and backfat thickness of Korean Native Pig which were sustained as different characteristics from other breeds in DNA constitution were identified by AFLP (amplified fragment length polymorphism). Specific marker was found in lower group of daily gain showing greater influence in males and transmitted with dominant inheritance. DNA marker related to daily gain was tremsmatted to all of progenies from parent. In backfat thickness, marker related to thinner group was inherited only from sire to all progenies, not from dam. These DNA markers with different effects by sex will be useful tools to improve important economic traits in Korean Native Pig.

한우의 근내지방도에 연관된 DNA Marker 의 규명

여정수,김재우,이문연,이지홍,강태석 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.5

DNA markers related to marbling score were identified using DNA fingerprinting with M13 probe and restriction enzyme HaeⅢ from 336 heads(147 heads of bull and 189 heads of steer) of Hanwoo. It was found that distinct 2 kinds of specific markers at 9.4 and 3.6kb showing significant difference for marbling score. DNA Marker groups having either 9.4, 3.6 or 9.4/3.6kb showed higher frequency of meat quality grade 1 or 2 than the group having none-marker. Also, the group having 9.4kb showed 94.4% of grade 1, indicating that this marker is valuable for the improvement of meat quality and was able to separate completely the lowest beef grade no.3.

중국의 서부 대개발에 따른 중국의 아황산가스 배출량과 주 배출 지역의 변화가 동아시아 황 침적량에 미치는 영향 : RAINS-Asia 모델을 통한 분석 Analysis Using the RAINS-Asia Model

여민주,김용표 한국대기환경학회 2005 한국대기환경학회지 Vol.21 No.1

It is widely accepted that, at present, the SO₂ emissions in China are not increasing thanks to the rigorous Chinese government policies. However, with the development of western China, it is possible that the SO₂ emission amounts might increase in regional scale. In this study, changes of sulfur deposition pattern and unprotected ecosystem in east Asia due to the sulfur emission pattern changes in China are studied by using the RAINS-Asia model. Five scenarios have been postulated to understand the effects on east Asia, especially, on Korea and Japan. It is found that the increase of SO₂ emission in western China might increase the total emission in whole China. And the amount of sulfur deposition from western China on east Asia would be higher than those from eastern China. The deposition amount of sulfur species on Asia is 3.2 Mt when SO₂ are emitted from western China only while 2.6 Mt from eastern China only. Generally, Korea and Japan are influenced more by emissions from eastern China than western China. However, if the SO₂ emissions from western China increase by 100% while those decrease by 10% in eastern China compared to the base case, the deposition amount of sulfur species on Korea and Japan would be higher than the base case. The fraction of unprotected ecosystem in Korea and Japan for the base case are 50 and 5%. However, if the emissions from western China increase by 100% while those decrease by 10% in eastern China, the fraction of unprotected ecosystem in Korea and Japan would be 52 and 6%.