서부 경남지역의 도살축우에 대한 주혈기생충의 역학적 조사

서명득,장동화 경상대학교 축산진흥연구소 1990 畜産振興硏究所報 Vol.17 No.1

장기도말법(臟器塗抹法)과 조직표본법(組織標本法)에 의(依)한 Babesia bigemina와 Babesia argentina의 형태학적감별(形態學的鑑別)에 관한 연구(硏究)

서명득,Suh, M.D.,Hoyte, H.M.D. 대한수의학회 1977 大韓獸醫學會誌 Vol.17 No.2

The smears and tissue sections of organs (liver, lung, kidney, heart, lymph-node, brain) were carried out to compare the detection of parasites in the splenectomized calves artificially infected with B. bigemina and B. argentina, respectively. The results obtained from this experiment were abstracted as follows: 1. In the calves infected with B. argentina the parasites were easily detected by the blood smears and tissue smears but, particularly, the detection of parasites in the kidneys and brain by tissue smears and tissue sections was greater useful. In this experiment, the packing of the parasite-infected red blood cells was found in the capillary vessels of the organs. 2. When the fresh infected-brain was kept at $5^{\circ}C$ refrigerator, Babesia argentina could be detected during 9 days from the brain smears. According to this results, we could do differentiate the parasites from the brain of the dead animal by the doubtful babesiosis in the field. 3. If we use the brain smears and brain sections in order to survey or detect Babesia infections of cattle in Korea, these methods could be worthwhile for the differential diagnosis of B. argentina and B. bigemina.

재래흑산양(在來黑山羊)의 흡충류(吸蟲類)(간질(肝蛭), 췌질(膵蛭), 쌍구흡충(雙口吸蟲))에 대(對)한 Nitroxynil(Trodax)의 구충효과(驅蟲效果)

서명득,Suh, Myung-Deuk 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2

The anthelmintic efficiency of nitroxynil(Trodax) at a dose rate of 10mg/kg was tested in naturally infected Korean native goat (Black goat) against Fasciola hepatica, Eurythrema pancreaticum, and Paramphistomum sp. The drug was administered with injection subcutaneouslly and the efficiency was measured by faecal examination microscopically. The results obtained were summarized as follows: 1. The anthelmintic efficiency of nitroxynil against Fasciola hepatica was shown 33.3% on day 7, 88.9% on day 14 and 94.4% on day 28 after the administration of the drug, respectively. The reduction rates of egg per gram of faeces (E. P. G.) against Fasciola hepatica in faecal examination were shown 86.8% on day 7, 97.7% on day 14, and 99.7% on day 28 postmedication. 2. The anthelmintic efficiency of nitroxynil against Paramphistomum sp. was shown 0% on day 7, 10.5% on day 14, and 36.8% on day 28 after the administration of drug. The reduction rates of E. P. G. against the parasite were shown 13.2% on day 14, and 39.4% on day 28 postmedication. 3. The anthelmintic efficiency of nitroxynil against Eurythrema pancreaticum was shown 0% on day 7, 16.6% on day 14, and 25% on day 28 after the administration of the drug.

반추수(反芻獸)의 내부기생충(內部寄生蟲)에 대한 Albendazole과 Ivermectin의 구충효과(驅蟲效果)

서명득,Suh, Myung-deuk 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2

The anthelmintic efficacy of Albendazo1e and Ivermectin against gastrointestinal nematodes, trematodes and cestode was tested in naturally infected Korean native goats. Albendazole was medicated at a dose rate of 10mg/kg of body weight orally and Ivermectin was injected at a dose rate of 0.2mg/kg of body weight subcutaneously. The efficacy was measured by faecal examinations on the day 7th and the day 14th :after treatment. The results obtained were summarized as follows : 1. The efficacy of Albendazole against trematodes and cestode was shown 91.7% in Fasciola hepatica, 68.8% in Paramphislomum spp., 66.7% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 2. The efficacy of Ivermectin against trematodes and cestode was shown 38.8% in Fasciola hepalica, 26.1% in Paramphistomum spp., 22,2% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 3. The anthelmintic efficacy of Albendazole against gastrointestinal nematodes was shown 97.7% in Haemonchus contorus and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Trichostrongylus spp., Strongyloides papillosus and Cooperia spp. 4. The efficacy of Ivermectin against gastrointestinal nematodes was shown 96.6% in Haemonchus contortus, 94.1% in Trichostrongylus spp. and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Strongyloides papillosus and Cooperia spp.

Babesia ovata의 순수분리와 형태학적 및 혈청학적 동정

서명득,Suh, Myung-deuk 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

This study was conducted to identify Babesia sp. strain isolated from the imported beef cattle, Aberdeen-angus heifers, in J farm of Jangsu Prefecture, Cheon-buk Province of Korea, morphologically, etiologically and serologically. Babesia sp. strain was purely isolated through the serial blood passages of three generations against splenectomized calves and one generation of blood passage against non-splenectomized calf(intact calf) by inhibiting the appearance of Theileria sergenti in the blood stream by means of injection of 20% oil pamaquine intramuscularly. In the splenectomized calves, the parasite multiplied markedly in blood stream soon after inoculation and parasitaemia was much severe. An elevated body temperature, anorexia, severe anaemia and icterus were observed clinically. Of three splenectomized calves, two were affected with haemoglobinuria and died. But in the non-splenectomized calf the clinical signs and parasitaemia were very mild. The means of the incubation period, the highest parasitaemia, the highest body temperature and the lowest PCV were 3.5 days, 41.1%, ${42^{\circ}C}$ and 9%, respectively, in the splenectomized calves. In calf erythrocytes Babesia sp. protozoa were polymorphic showing the round, oval, ameboid, piriform and paired piriform etc. The sizes of protozoa were $2.51{\sim}3.91{\times}1.32{\sim}2.19{{\mu}m}$ ($3.20{\times}1.60{\mu}m$). Serologically the isolated Babesia sp. were compared with other parasites, B. ovata, B. bigemina, B. bovis, T. sergenti, A. marginale and A. centrale by using the complement fixation(CF) test. As a result, the antibody titer between the homologous species were high. It was two tubes or more in the CF test. From the results obtained above the pure isolated Babesia species was identical with Babesia ovata Minami and Ishihara, 1980.

ELISA를 이용한 돼지 톡소플라스마병의 조기 진단에 관한 연구

서명득,장동화,주후돈,Suh, Myung-deuk,Jang, Dong-hwa,Joo, Hoo-don 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4

This study was conducted to evaluate the possibility of application of a microenzyme-linked immunosorbent assay(micro-ELISA) for the serodiagnosis of specific toxoplasma antibodies in swine sera and this test was performed as a microplate system by coating the polystyrene plates with toxoplasma soluble antigen, incubated serially diluted sera, then added horse radish peroxidase labelled goat anti-swine IgG(r) conjugate followed by o-phenylenediamine as substrate. The color development by enzyme-substrate reaction was determined by the photometric reading [ELISA reader at 490nm (OD)] and visual reading. The soluble antigen was prepared from the tachyzoites in mouse peritoneal cavity. A total of 1,200 swine sera from pig slaughter-house and a total of 116 swine sera from pig breeding station (S-C farm) were tested for the detection of antibodies to Toxoplasma gondii. The results obtained were summarized as follows: 1. The optimal reactions of indirect ELISA for the test sera were determined by the dilution of antigen 1:256 and 1:3,200 of horse radish peroxidase conjugate [anti-swine IgG(r)]. 2. The specific togoplasma antibody(IgG) in pigs infected with Tp artificially were detected as the serum titers of 1:64 or 1:128 at one week postinfection. 3. Of a total of 1,200 swine sera from pig slaughter-house 505 samples of sera were detected as positive (42.1%) and of a total of 116 swine sera from S-C pig breeding station 68 samples of sera as positive (58.6%). 4. The specific antibody(IgG) detection rates against a total of 1,200 test sera from pig slaughter-house were not significant between male (43.1%) and female (40.7%). 5. The indirect ELISA was proved to be a sensitive and specific procedure for the serodiagnosis of swine toxoplasmosis and also evaluated as an effective screening test for the large scale of test samples in laboratory.

꿀벌의 Nosema병에 관한 연구 2. 인공감염봉군에 대한 실험실내 치료시험

서명득,강영배,김창섭,김동성,Suh, Myung Deuk,Kang, Yung Bai,Kim, Chang Sup,Kim, Dong Sung 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.2

Experimental approaches on the effectiveness of thimerosal to control growth of Nosema apis (Zander, 1909) were carried out in the rearing honey bees. The rearing honey bees were artificially infected with various levels of spore isolated from local honey bees. The results obtained were summarized as follows: 1. In the experiments of therapeutic chemicals for Nosema disease, 0.01% and 0.02% thimerosal of sucrose-honey mixture was the most effective agent but the each concentration of amprolium, furazolidone, hygiene, sulfadimethoxine and terramycin did not show the any effects 2. It showed very high therapeutic effectiveness (over than 90%) that the treatment of three times every other day after the treatment of three times consecutive every day with 0.01% thimerosal, or the treatment of three times consecutive every day with 0.02% thimerosal. 3. When 0.02% thimerosal was administered three times consecutive every day to honey bees at the 4th day before artificial inoculation of N. apis, it showed very high degree (100%) of prevalence control effectiveness, and it also showed high degree (over than 90%) in administration at the 7th day before, and over than 80% at the 10th day before. Then authors found that thimerosal has the prevalence control effectiveness as well as the treatment effectiveness. 4. In the rearing honey bee colony, 0.02% thimerosal showed the high degree (over than 80%) of therapeutic effectiveness with the various levels which contained from the light decree of infection to the severe degree of it.

꿀벌의 Nosema 병(病)에 관한 연구(硏究) 1. 원인체분리(原因體分離) 및 감염실태조사(感染實態調査)

서명득,김창섭,강영배,김동성,Suh, Myung Deuk,Kim, Chang Sup,Kang, Yung Bai,Kim, Dong Sung 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2

The experiments were conducted to isolate the etiogical agent and to survey the distribution of Nosema disease in honey bees. The results obtained were as follows: 1. The etiological agent of the so-called "crawling disease" in honey bees characterized by the symptoms of crawling, diarrhea, and enteritis etc. was first isolated and identified with Nosema apis (Zander 1909) in Korea. 2. 455 colonies were randomely sampled and surveyed in 4,766 bee colonies out of 56 apiaries and 51 colonies (11.2%) out of 455 bee colonies were infected with N, apis. 3. Infection rates according to the period of honeyflow as follows: Brassica napus (Apr.): 25/130 colonies (18.4%) Rohinia pseudoacacia (May) : 8/55 colonies (14.%) Trifolium repels(Jun.): 15/99 colonies (13.6%) Castanea crenate (Jul.): 3/46 colonies (6.5%) Lespedeza bicolor(Aug.): 0/60 colonies (-) Fagopyrumesculentum(Sept.) & Perilla frutescens(Oct.) 0/65 colonies (-) 4. The typical clinical signs of Nosema disease were appeared on loth day after N. apis was orally administered with the level of $16{\times}10^4$ spores/ml to the healthy adult bees. Spores could be harvested with the level of $121{\sim}236{\times}10^4$ spores/ml on 10th day and $392{\sim}429{\times}10^4$ spores/ml on 15 days after infection. 5. In adult honey bees infected with N. apis artificially the 50% lethal day of life-span was 9 to 10 days and 100% lethal day was 16 to 19 days. However, in the control 50% lethal day was 19 to 23 days and 100% lethal day was 31 to 33 days.

돼지 Toxoplasmosis 의 간접 적혈구응집반응과 피내반응에 관한 연구

서명득,장두환,Suh, Myung Deuk,Jang, Du Hwan 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1

Hemagglutinating antigen of Toxoplasma gondii was prepared and purified by the method of a slight modification of Tsunematsu, and the preparation of the skin test antigen (toxoplasmin) was made by means of acetone-ether treatment described by Nobute et al. With these antigens the passive hemagglutionation and skin tests were performed for the diagnosis of swine toxoplasmosis by using artificially infected pigs. The results obtained were summarized as follows: 1. The hemagglutinating antibody and the skin test antibody were demonstrated one and three weeks after infection, respectively. And these antibodies were maintained over nine weeks after infection. 2. The antigenicity of hemagglutinating antigen was stable when it was kept in frozen state, while was unstable in a liquid state. 3. Freeze-dried skin test antigen (toxoplasmin) was stable for two months or more if it was kept at $5^{\circ}C$ and room temperature, but in the liquid or reconstituted state it was unstable. 4. Freeze-dried skin test antigen could be preserved without loss of antigenicity for more than two months. 5. Passive hemagglutination test could be applied effectively at the early phase of the disease process and skin test at later phase, mainly for epidemiological survey. However, by combiniation of these methods, the more accurate results could be obtained.

Babesia gibsoni의 적혈구내 배양법과 진단법 개발에 관한 연구 1. Babesia gibsoni 진단을 위한 간접형광항체법(IFAT)과 효소표지면역검사법(ELISA)

서명득,신용승,Suh, Myung-deuk,Shin, Yong-seung 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3

Indirect fluorescent antibody test(IFAT) and enzyme-linked imuunosorbent assay (IgG-ELISA) as serological diagnostic tools were conducted to evaluate the usefulness for diagnosis of canine babesiosis infected with Babesia gibsoni in domestic various dog breeds, american pit bullterrier, military shepherd, and mongrel dogs. The results obtained from this study were abstracted as follows. The nonionic detergent Triton X-100 and absorbent bio-bead $SM_2$ were useful reagents for the preparation of pure merozoite antigen of B gibsoni to be used in ELISA. The optimum reaction in ELISA was shown when the protein concentration of ELISA antigen was measured as 625ng/ml and the conjugate concentration was diluted into 1/6000 fold. The average OD value of ELISA in sera determined with negative responses in IFAT was measured as $0.255{\pm}0.051$(490nm) and the cut - off value of OD was determined as 0.399(490nm). The serum antibodies in both of IFAT and ELISA were detected on one week after artificially infected with B gibsoni and these high antibody titers, 512X in IFAT and 1024X in ELISA, were long lasted until 15 weeks after infection. The reproducibility of reaction and stability of the antigen absorbed microtitration polystyrene plate preserved in $4^{\circ}C$ refrigerator and $-20^{\circ}C$ freezer, respectively could be lasted until 135 days after storage. The positive rates in IFAT by dog breeds were shown 8.1%(60/744 heads) in mongrel dogs, 81.3%(78/96 heads) in american pit bullterrier and 15.6%(15/96 heads) in military shepherd, while the positive rate in ELISA shown 17.6%(131/744 heads) in mongrel dogs, 83.3%(80/96 heads) in american pit bullterrier and 36.5%(35/96 heads) in military shepherd, respiectively. In the total of 936 heads surveyed with IFAT and ELISA the positive rates in IFAT and ELISA were 16.4%(153/936 heads) and 26.3%(246/936 heads), respectivily. Agreement of reactions between IFAT and ELISA was shown 82.4% in 936 dog sera. The specificity and sensitivity of ELISA reaction were 83.5% and 76.5%, respectively. From the conclusion obtained in this study it was evaluated that IFAT and ELISA were useful as highly specific, sensitive and stable serelogical tools for the diagnosis of canine babesiosis in Korea.