The Effect of Rosiglitazone on the Cell Proliferation and the Expressions of p27 and Skp2 in<i>Helicobacter pylori</i>Infected Human Gastric Epithelial Cells

Kim, Sung-Soo,Cho, Young-Seok,Kim, Hyung-Keun,Shin, Ok-Ran,Chae, Hiun-Suk,Choi, Myung-Gyu,Chung, In-Sik The Korean Society of Gastroenterology 2010 대한소화기학회지 Vol.55 No.4

<P>Background/Aims: Ligands for peroxisome proliferator-activated receptorgamma (PPARgamma), a member of the ligand-activated nuclear receptor superfamily, exhibit anti-tumoral effects and are associated with de novo synthesis of proteins involved in regulating the cell cycle and cell survival/death. Helicobacter pylori (H. pylori) is an etiologic agent for gastric adenocarcinoma, and raises the cell turnover of gastric epithelium. The aim of this study was to investigate the effect of PPARgamma ligand rosiglitazone on the cell proliferation and the expressions of p27 and Skp2 protein in H. pylori infected gastric epithelial cells. Methods: We examined the expression of PPARgamma by Western blot in H. pylori infected AGS human gastric epithelial cells. The effect of rosiglitazone on the survival of H. pylori infected AGS cells was assessed by cell viability assay. After the treatment of rosiglitazone in H. pylori infected AGS cells, the expressions of p27 and Skp2 were assessed by Western blot. Results: The expression of PPARgamma protein was increased in H. pylori infected AGS cells. Cell growth was inhibited and decreased in dose- and time- dependent manner in H. pylori infected AGS cells treated with rosiglitazone. A decrease in Skp2 expression and a reciprocal increase in p27 expression were found in dose- and time-dependent manner in H. pylori infected AGS cells treated with rosiglitazone. Conclusions: Rosiglitazone inhibited the growth of H. pylori infected AGS cells. Rosiglitazone attenuated Skp2 expression, thereby promoting p27 accumulation in H. pylori infected human gastric epithelial cells. Further studies will be needed to find the effects of accumulation on cell turnover in H. pylori infection and the role in the H. pylori-associated gastric carcinogenesis.</P>

정신지체 특수학교 전공과 및 전환교육의 운영실태와 개선방향

조인수,도금숙,전보성 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.1

본 연구의 목적은 정신지체 특수학교의 전공과 및 전환교육의 운영실태와 개선방향을 탐색하는데 주안점을 두었다. 전공과 및 전환교육의 운영실태는 16개 영역으로 조사하고 문제점과 개선방향은 10개 영역을 중심으로 세부적인 내용을 조사 분석하였다. 여기서 조사분석된 내용은 정신지체 직업 · 전환교육에 좋은 지침이 될 수 있을 것이며 타장애영역 학생들의 직업진로 및 전환교육에 실질적인 도움을 줄 수 있을 것이다. The aims of this study are finding out the reality of the operation of the majored subjects and problems and finding out the improved methods about them. And 1 surveyed special teachers who are attending 17 special schools in Korea in which have the majored subjects about the reality of operating them and improved methods about them. First, about surveying the reality of operation, as to the majored subjects, they are mainly teaching students in the form of work training, guiding job, preparing job and daily Iiving skills through classes. The selection of majored subjects conceming jobs are made through school's direction and the cooperation among teachers, students and parents, and teaching jobs are now changed or will be changed because of the maladjustment to student' s aptitude, the assessment on their job aptitude is not being made now. There are many qualified teachers who have the certification in special school and they think that their roles as directors, guiders are important. The majoring students don’t have enough practicing places and factories. Even though they are received training about job in school, they get jobs unrelated with their received training. And they have verγ low employment as a whole. Though the transitional education program whose aims are selection in jobs, employment and enjoying jobs, it is started especially in the middle and high school courses. And it is thought that official and individualized education program is very important, also needed the cooperation among related authorities and it’s major program is used as the programs on job training. programs on job training. Next, as to the problems and improved methods, the problems about the majored subjects are the unrelatedness with job training and unreality and only one line of selection in job training. The problem about the transitional education program is the shortage in social skills, in the basement of job training and in carrγing out ability. The professional ability which is required in job guiding teacher is job training guide, many methods, the ability about assessing job aptitude, and testing their ability. The construction of the curriculum is not applying the guided curriculum rather than transforming the curriculum according to the atmosphere, and their school year should not be stuck to any one standard but be varied according to their ability and improvement. In the operation of majored subjects, it is necessarγ for them to provide real career education, the opportunity to receive various job training and the supported employments in which they can be included in their society actively.

전환교육 관점에서 본 정신지체아 여가 교육 활동의 이론적 탐색

조인수,류현주 한국정신지체아교육학회 2002 지적장애연구 Vol.4 No.-

전환교육의 영역이 최근 협의의 영역에서 포괄적인 영역으로 이동함에 따라 여가 및 레크리에이션에 관한 중요성이 부각되고 있다. 이에 본 연구는 정신지체 학생에게 여가교육이 차지하는 중요성에 대해 살펴봄으로써 우리나라 정신지체 학교교육 현장에서 여가교육 프로그램을 계획ㆍ구성하기 위한 기초연구이다. 연구방법으로 관련 문헌을 중심으로 고찰했다. 선행연구들(Schleien & Ray, 1998; Hughes & Fullwood, 1985; Moon & Bunker, 1987; Meyer & Evans, 1989; Heyne, Schlein & Rynders, 1997)은 정신지체 학생들이 일반 학생들에 비해 실패감을 많이 경험하고 자아 개념과 동기수준 결여 및 사회적 상호작용 결핍을 보이기 때문에, 인지적 요구가 적은 여가활동을 통해 자아개념과 자신감, 정상화 촉진, 부적절한 행동 감소 등의 교육적 가치를 강조하여 여가교육을 중요한 영역으로 다루고 있다. 연구를 통해 얻은 결론은 학교 현장에서 여가교육을 실시할 때 학교 교육과정 내에서 실시할 수 있는 방법과 여가프로그램을 구성할 시 정신지체 학생에게 적합한 여가 활동 프로그램을 고려하여 시행해야 한다. Recently, as the domains of transition education move it from a narrow sense to comprehensive sense, importance of leisure and recreation in domains of transition education be highlighted. Therefore, this study is a basic search that may use for make a planning and constitution of leisure education programs at school for mental retardation in korea, as we look into importance of leisure education for children with mental retardation described in literature. As preceding literature(Schleien & Ray, 1998; Hughes & Fullwood, 1985; Moon & Bunker, 1987; Meyer & Evans, 1989; Heyne, Schlein & Rynders, 1997) showed that children with mental retardation have a more failure experience, deficient in self concept, motive and social interrelation than normal children stress value of education of leisure activities, because leisure activities demanded less intellectual accelerate for children with mental retardation to self concept, confidence, normalization, reduction of inadequacy behavior. In conclusion, when implementation to leisure education in the field of school, we should be several considerations that how to act in school curriculum and leisure activities programs fitted to children with mental retardation.

In-hospital mortality in patients with STEMI admitted during off hours

( Sung-Soo Kim ),( Myung Ho Jeong ),( Jeong Gwan Cho ),( Young Keun Ahn ),( Jong Hyun Kim ),( Shung Chull Chae ),( Young Jo Kim ),( Seung Ho Hur ),( In Whan Seong ),( Taek Jong Hong ),( Dong-hoon Choi 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Conflicting results exist on the outcome of off hours PCI in ST elevation myocardial infarction (STEMI). However, there were only a few studies that have focused on the clinical characteristics and outcomes of off hours PCI in STEMI. So, we studied the clinical characteristics and hospital mortality in STEMI patients treated with primary PCI during regular hours (weekdays 9:00 AM to 6:00 PM) versus off hours Weekdays 6:01 PM to 8:59 AM, weekends, and holidays) in Korea Acute Myocardial Infarction Registry. We analyzed in hospital and one year mortality among 5,665 consecutive ST segment elevation myocardial infarction patients treated with primary PCI between November 2005 to January 2008. Total 2,848 (50.2%) patients were treated during off hours. Baseline finding were similar, although regular hours patients were older. Median symptom to balloon time (304 min, IQR 175 to 750 vs. 270 min, IQR 145 to 551, p=0.001) were longer for regular hours primary PCI. Median door to balloon time (71min, IQR: 48 to 132 vs. 59 min, IQR 39 to 110 min, p=0.001) were longer for off hours pPCI. Also, Cardiac enzyme such as Max CK-MB (212.1±299.3 vs 194.7±303.4, p=0.031) and max TnI (72.6±239.5 vs. 58.9±94.4, p=0.013) were increased in off hours pPCI. However, unadjusted in hospital (6.0% off hours vs. 6.0% regular hours, p=0.946) and one year cardiac mortality (11.3% off hours vs. 11.7% regular hours, p=0.661) were comparable. In multivariate analysis, off hours primary PCI did not predict an adverse outcome. In conclusion, when primary PCI was performed within an appropriate reperfusion strategy, the clinical effectiveness of either off hours or regular hours pPCI is comparable.

역할놀이활동이 정신지체학생의 자기결정능력에 미치는 효과

조인수,최소연 국립특수교육원 2004 특수교육연구 Vol.11 No.2

본 연구는 역할놀이 활동이 정신지체학생의 자기결정능력에 미치는 효과를 밝히고자 수행되었다. 연구의 대상은 경상북도에 소재하고 있는 K 특수학교 중, 고등부에 재학중인 정신지체 학생 40명이며, 실험집단과 비교집단에 20명(남 10, 여 10)씩 배치하였다. 실험집단은 역할놀이활동 프로그램을 14주동안 주 3회씩 하루 80분 동안 실시하였다. 본 연구의 결과는 다음과 같다. 역할놀이 활동은 정신지체학생의 자기결정 능력 중 자율성, 심리적 역량, 자아실현 능력을 향상시키는데 도움을 준다. 따라서 역할놀이 활동은 정신 지체학생의 자기결정능력을 증가시키는데 긍정적인 효과가 있다고 할 수 있다. 연구자가 재구성하여 사용한 프로그램은 교육현장에 교수적 함의를 제공할 수 있을 것이다. This study was conducted to investigate the effect of a role-play activity on the ability of self-determination for students with mental retardation. The subject of this study was 40 students with mental retardation who were enrolled in the level of middle and high school in K special school, Kyengsangbuk-Do. An experimental and a control group included 20 participants each (10 boys 10girls). Participants in experimental group carried out the program of role-play activity for three 80 minutes session per week over a period of 14 weeks. The results of this study were as follows: First, the role-play activities had an effect on increasing behavioral autonomy in the ability of self-determination for students with mental retardation. Second, the role-play activities had an effect on increasing the acting in a psychologically empowered manner in the ability of self-determination for students with mental retardation. Finally, the role-play activities had an effect on increasing the improvement in self-realization in the ability of self-determination for students with mental retardation. Therefore, it may be concluded that the role-play activities is effective to improve the ability of self-determination for students with mental retardation.

우리나라 연 작업자들의 연폭로 수준에 관한 연구

조인숙,김용배,리갑수,김화성,황규윤,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

To assess lead exposure in Korean lead workers, 2047 male lead workers in 17 lead using industries were studied. Study population were divided into 4 occupation categories such as storage battery industry(type 1), secondary smelting and litharge making industries(type 2), PVC stabilizer making industries(type 3) and primary smelting and related industries (type 4). Blood lead and zinc protoporphyrin in whole blood (ZPP) were selected as an index of lead exposure. Hemoglobin and hematocrit were also checked. Fifteen questionnaires of lead related symptoms were provided to all lead workers and filled up by themselves and confirmed by medical doctors. Personal variables such as age, work duration, smoking and drinking habits were also collected. the results were as follows. 1) The mean blood lead, ZPP and hemoglobin of all lead workers were 29.7+12.6㎍/100㎖, 56.5+38.6㎍/100㎖ and 14.6+1.3 gm/100㎖ respectively. 2) While the mean blood lead of lead workers in type 1, 2, 3 and 4 were 28.6+11.6㎍/100㎖, 46.2+15.3 ㎍/100㎖, 46.8+12.1 ㎍/100㎖ and 26.2+10.2 ㎍/100㎖, the mean value of blood ZPP of those workers were 53.6+32.6 ㎍/100㎖, 108.1+87.1 ㎍/100㎖, 98.9 +54.9 ㎍/100㎖ and 45.8+19.5 ㎍/100㎖ respectively. 3) The percents of lead workers whose blood lead were 40-59.9 ㎍/100㎖ and over 60.0 ㎍/100㎖ were 17.6% and 2.1% respectively. Those whose blood lead were 40-59.9 ㎍/100㎖ and over 60.0 ㎍/100㎖ were 15.18% and 0.96% for type 1, 43.59% and 21.79% for type 2, 58.89% and 12.22% for type 3 and 11.82% and 0% for type 4 respectively. 4) The percents of lead workers whose blood ZPP were 100-149 ㎍/100㎖ and over 150 ㎍/100㎖ were 5.17% and 3.17% respectively. Those whose blood ZPP were 100-149 ㎍/100㎖ and over 150 ㎍/100㎖ were 4.66% and 2.04% for type 1, 10.26% and 25.64% for type 2, 23.33 and 13.33% for type 3 and 1.28% and 0.32% for type 4 respectively. 5) The prevalence rate of smoking and drinking of male lead workers in all lead industries were 70.8% and 71.0% respectively. While the mean blood lead of smoker, non-smoker, drinker and non-drinker were 30.24+12.58 ㎍/100㎖, 28.35+12.7 ㎍/100㎖, 30.24 ㎍/100㎖ and 28.74 ㎍/100㎖ respectively, the mean blood ZPP of those were 55.7+37.1 ㎍/100㎖, 58.4+41.9 ㎍/100㎖, 55.7+36.5 ㎍/100㎖ and 58.4+43.3 ㎍/100㎖ respectively. 6) While the correlation of hematocrit corrected blood lead with other lead exposure variables were higher than non-corrected blood lead, the correlation of log-transformed ZPP with other lead exposure variables were higher than non-corrected blood ZPP. 7) The most frequent symptom among 15 lead related symptom questionnaires was 'general fatigue' and the next one was 'feeling irritated with disturbance' and least one was 'acute abdominal pain around the umbilicus' in all lead workers. The overall prevalence of lead workers in storage battery industry and primary lead smelting industry were higher than secondary lead smelting and PVC stabilizer making industries. 8) The prevalence of lead related symptoms was a little higher in 13 symptoms in smokers than non-smokers and in 14 symptoms in drinker than non-drinkers, but they were not statistically significant. 9) Stepwise multiple regression analysis, using overall symptom prevalence as a dependent variable blood lead, blood ZPP, hemoglobin, age, work duration, drinking and smoking habits as independent variables, indicated that smoking habit, drinking habit and age were significantly contributed to the dependent variable. 10) The slopes of simple linear regression of blood lead (independent variable) with blood ZPP and log-transformed ZPP as dependent variable were different among 4 types of lead industries. The slope of lead industries with low mean blood lead were less steeper than those with high mean blood lead.

전기화상후 Prostaglandin F2a와 Thromboxane B2의 증가에 대한 Flurbiprofen, Prednisolone 및 Gabexate mesilate의 억제효과

조병채,김인규,변진석,백봉수 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

This study was conducted to clarify the inhibitory effect of flurbiprofen, prednisolone, and gabexate mesilate on the synthesis of prostaglandin F2a and thromboxane B2 in the tissue of electrical burn. one hundred sixty Sprague-Dawley rats, 250gm of body weight in average, were divided into 4 groups of control, flurbiprofen, prednisolone and gabexate mesilate administred group. Fourty rats of the each group were subdivided into 1st, 2nd, 4th, 7th postburn day with 10 rats in each subgroup. Ten rats were used for normal values of prostaglandin F2a and thromboxane B2. Flurbiprodfen 30mg/kg was given per mouth, and prednisolone acetate 5mg/kg and gabaxate mesilate 50mg/kg were administred intramuscularly 5 minutes after burn injury and then once a day. All but bone tissue of within 2cm area of the current entry were harvested and ground into homogenate. The levels of prostaglandin F2a and thromoboxane B2 were measured by Radioimmunoassay. The results were as followings: In normal rat tissue, the level of prostagland F2a and thromboxane B2 were 15.8±1.8 and 17.6±2.8ng/mg protein in each. In the control group the levels of both prostaglandin F2a and thromboxane B2 increased abruptly to 5 times and 7 thimes in the first day and maintained the same level in the second day. After then they rapidly decreased and returned to a near normal level in the fourth day. The increase of thromboxane B2 was more remarkable than that of prostaglandin F2a. In the flurbiprofen group, the level of thromboxane B2 was not inerease but kept at a normal level. The average level of prostaglandin F2a in the post-burn first and second day was 59.5% lower than of the control group. The average level on the post-burn first and second day was 61% lower in thromboxane B2 and 33% lower in prostaglandin F2a in the prednisolone group, and 68% lower in throboxane B2 and 48% lower in prostaglandin F2a in the gabexate mesilate group, compared to the control group. These findings suggest that electrically injured tissue is vigrously progressive due to rapid increase of thromboxane B2 and prostaglandin F2a, as inflammatory mediators, in the post-burn first and second day, is nonprogressive after the post-burn fourth day. The flurbiprodfen was the most effective of the three drugs(flurbiprofen, gabexate mesilate, prednisolone in order of effectiveness) to suppress the synthesis of thromboxane B2 prostaglandin F2a of the electrieally injured tissue.

정신지체특수학교 교사의 직무연수 요구 수준 분석

조인수,박정식 대구대학교 특수교육재활과학연구소 2004 특수교육재활과학연구 Vol.43 No.2

이 연구는 정신지체특수학교 교사들의 직무연수 요구수준을 밝히는 조사연구이다. 연구대상은 228명으로 설립별, 성별, 과정별, 경력별로 분석하였으며, 설문지구성은 연수과정 운영, 연수 개선 및 유인체제 2개영역 11개 문항으로, 약 3개월에 걸쳐 조사되었다. 연구결론은 첫째, 정신지체교육교사들의 직무연수 연수과정 및 운영에 대한 요구는 매우 다양하다. 둘째, 직무연수에 있어서 개선점 및 유인체제 등에서 제시된 내용은 타 직무연수에 많은 참조가 되리라 믿는다. 특히, 현장교사들을 위한 시간과 장소의 제약을 최소화하는 실천적 연수와 연수내용과 방법, 승진 및 전보에 관한 공정성 확보 및 평가방법의 개선이 필요하고, 정신지체아교육 교사들에게는 교수적 함의 개선에 실질적으로 도움을 줄 수 있는 연수가 되어야 한다. 본 연구에서 제시된 구체적 내용은 앞으로의 직무연수 운영과 개선에 많은 도움을 줄 수 있는 것이다. The purpose of this study is to reveal level of in-service education and training needs for special school teacher for the mentally retarded with regard to in-service education and training process·administration, and improvement·inducement system. The conclusion be drawn from this study are as follow; First, we have consideration on make a selection of training content and how to do it in order to elevate effects of training. Second, while special school teacher for the mentally retarded see the necessity of in-service education and training under the new circumstance change, it also open it to the teacher who desired to be received in-service education and training. Third, training always must not be restricted by time and place. Fourth, we not only should improve dissatisfaction factors, but reinforce inducement system in order to activate training. Fifth, we should elevate confidence of training evaluation through fair evaluation, also need reform evaluation method

일부섬유업체 근로자들의 우울상태에 관한 연구

한인수,김수영,조영채 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

In order to investigate the relationship among sociodemographic and job related factors, health practices and depressive mood, this survey was performed by self-reported questionnaire on 376 textile industrial workers from August to September 1994, which included items on 8 health practice and Zung Self-rating Depression scale(Zung SDS). The results were summarized as follows ; 1 The Zung SDS scores were higher in female(50.02±6.47) and blue color(48.88±6.55) than male(46.73±6.85) and white color(46.06±7.56) and it was very significantly difference by Wilcoxon test. 2. The severity of depression mood were 9.0% of normal(20∼39 SDS), 33.2% of mild(40∼47 SDS), 45.3% of moderate(48∼55 SDS) and 12.5% of severe(56 and more), respectively. 3. The Zung SDS scores according to the demographic variables were found to be significantly high score in the low educational level group in male, but it was high score in 20 or less age group, unmarried group and low educational level group than others in female. 4. The Zung SDS scores according to the job related variables were found to be significantly high scores in the shift work group, the longer overtime work group and dissatisfaction group to the job satisfaction in female, but it was not significantly difference in male. 5. The Zung SDS scores according to the Health practice Items were found to be significantly related to low depressive socre in male as following 3 items : ① habitual physical activity ② moderate alcohol consumption ③ obesity 10.0∼19.9%. The following 4 items were found in female to be significantly related to low depressive score : ① sleeping regularly 8 hours or more per day ② eating breakfast and snacking regularly ③ no smoking ④ obesity -9.9∼9.9% 6. The Zung SDS Scores were decreased according to increasing the health practice index in sex and job contents.

Dibenzo-18-Crown-6 함침 액막에 의한 Tyrosine 의 이동

조정국,우인성,강안수 明知大學校 産業技術硏究所 1985 産業技術硏究所論文集 Vol.4 No.-

Cation of alkali metal form complexes selectively with carrier in organic phase. With these characteristics, complexes were combined with amino acid anions and the diffusional flux of amino acid were facilitated. The transfer rate of tyrosine were studied with the change of stirring speed, initial concentration of tyrosine, carrier concentration, cations of alkali metal and time in order to in creased it. The experiment was carried out to transfer tyrosine from aqueous phase consisting of electrolytes and tyrosine to another aqueous phase consisting of KOH across a immobilized liquid membrane containing cyclic polyether, dibenzo - 18 - crown - 6 dissolved in toluene. The experimental results showed that the transfer rate of tyrosine was increased when the initial concentration of tyrosine and carrier concentration were high. In case of electrolytes added in aqueous phase the transfer rate was increased with the higher concentration of KBO₃ and the smaller concentration difference of hydroxyl ions between each phase. And the transfer rate was linear with concentration difference of tyrosine between each phase.