외과계 감염증에 대한 Sulperazon(Sulbactam/cefoperazone) 및 Cefoperazone의 임상효과 비교연구

박규주,노동영,김상준,권오중,정중기,홍인규 대한화학요법학회 1990 대한화학요법학회지 Vol.8 No.2

Despite recent advances in development of antibiotics and antiseptic techniques, infection still remains to be one of the major problems in the surgical field. Due to development of bacterial resistance to commonly used antibiotics, there is always a need to develop a new antimicrobial agent which would overcome such resistance. Sulperazon (Sulbactam/Cefoperazone) is a combination drug of potent β-lactamase inhigitor, sulbactam, and cefoperazone which has broad antibacterial spectrum, which was developed to combat against β-lactamase producing microorganisms. In an effort to evaluate the efficacy and safety of sulperazone, we have randomly divided 40 patients (12 with perforated appendicitis, 10 with peritionitis, 6 with GB empyema, 6 with periproctal abscess, 3 with wound infection, 1 liver abscess, 1 pancreatic abscess, and 1 acute cholangitis) into 2 groups (20 patients in each group). One group received daily dose of 2 to 3 grams of sulperazon and the other group received daily dose of 2 to 3 grams of cefoperazone. Following results were obtained : 1. Clinical effect of sulperazon was cure in 16 patients (80%), improvement in 3 patients (15%), compaired to cure in 14 patients (70%), improvement in 4 patients (20%) for the cefoperazone group. 2. Using disc diffusion method, sulperazon was sensitive to all of the isolated microorganisms except β-hemolytic streptococci, which was moderately sensitive. Cefoperazone was sensitive to only E. coli, Pseudomonas aeruginosa and Serratia. 3. Microbiological effect of sulperazon was eradication of offending microorganisms in 16 patients (80%), compared to 14 patients (70%) in Cefoperazone group. 4. Overall clinical efficacy of sulperazon was 95%, compared to 80% for cefoperazone. 5. Adverse effects due to sulperazon was nausea and diarrhea which occurred in 1 patient respectively. 2 patients receiving cefoperazone experienced diarrhea. It can be concluded that sulperazon is a safe and effective antibiotic agent which can be used in surgical infection patients.

In-vitro Antioxidant and Rat small Intestinal α-glucosidases Inhibitory Activities of Bioactive ingredients isolated from Colored Rice Keun-nun-Jami (Oryza sativa L.) Germ Extracts

Tae-Yang Kim,Hanna Kang,Jin-a Yu,Sung-Gil Hong,Su-Noh Ryu,Young-In Kwon 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

만성질환자들의 한의약의료서비스 이용과 결정요인 -제주도 보건소이용 환자를 중심으로-

오종수,한동운,임문혁,홍용석,이영호,노홍인,Oh, Jong-Soo,Han, Dong-Woon,Im, Mun-Hyuk,Hong, Yong-Seok,Lee, Young-Ho,Noh, Hong-In 대한예방한의학회 2009 대한예방한의학회지 Vol.13 No.3

Background : Traditional Korean medicine(TKM) has gained in popularity among chronic patients in recent years. The use of TKM among patients with chronic diseases is common, with about two thirds of patients using some form of TKM in Korea. Objectives : The purpose of this study is to analyze the use of TKM and determine what factors affect to use TKM among patients with chronic diseases. The study also aims to provide information on TKM therapies and assist therapy selection among various TKM therapies for patients with chronic diseases. Methods : The design of the study was descriptive cross-sectional, and data were collected using a 21-item questionnaire. This study was conducted with subjects with confirmed diagnosis of chronic diseases, who live in Jeju Province, Korea. Results : Among the participants, past or current TKM use was 66.7%, with a statistically significant difference in gender and level of health status groups(p<0.05), but no difference in age, marital status, education, occupation, and income groups. The most common therapies of TKM used by the patients included acupuncture(51.1%), physiotherapy(16.8%), cupping(13.5%), and herbal medicine(4.8%). The main benefits from TKM perceived by the patients were chronic diseases management and health promotion. In a logistic regression analysis, significant influencing factors related to TKM use were gender, family income level, the extent of recognition of efficacy, heath status, and health security program. Conclusions : In this study the socio-demographic and health status, recognition of TKM efficacy factors associated with TKM use among patients with chronic diseases were similar to those found in the general population. The findings suggest that due to the relatively high use of TKM among patients with chronic diseases in Korea, this topic should be taken into account in the development of a holistic approach for patients with chronic diseases and an efficient chronic disease management system. Additionally proactive and consistent management of TKM is necessary in the health care system in Korea.

Anti-inflammatory Effect of Columbianetin on Activated Human Mast Cells

Jeong, Hyun-Ja,Na, Ho-Jeong,Kim, Su-Jin,Rim, Hong-Kun,Myung, Noh-Yil,Moon, Phil-Dong,Han, Na-Ra,Seo, Jae-Uk,Kang, Tae-Hee,Kim, Jae-Joong,Choi, Youngjin,Kang, In-Cheol,Hong, Seung-Heon,Kim, You-Ah,Seo, Pharmaceutical Society of Japan 2009 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.32 No.6

<P>In the present study, we extracted <I>Corydalis heterocarpa</I> with various solvents in order to find the bioactive constituents that demonstrated anti-inflammatory effects. We isolated the active compound, Columbianetin. Anti-inflammatory effect of Columbianetin has been reported but the precise effects of Columbianetin in experimental models have remained unknown. In the present study, we investigate the effect of Columbianetin on the production of histamine, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α and expression of cyclooxygenase-2 (COX-2) by using the human mast cell line (HMC-1). Various concentrations of Columbianetin were treated before the activation of HMC-1 cells with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore, A23187. PMA plus A23187 significantly increased IL-1β, IL-6, IL-8, and TNF-α production compared with media control (<I>p</I><0.05). We also show that the increased cytokines IL-1β, IL-6, IL-8, and TNF-α level was significantly inhibited by Columbianetin in a dose-dependent manner (<I>p</I><0.05). Maximal inhibition rates of IL-1β, IL-6, IL-8, and TNF-α production by Columbianetin were about 102.6%, 101.1%, 95.8%, and 103.9%, respectively. Columbianetin inhibited expression of COX-2. In addition, the effect of Columbianetin was investigated on the histamine release from HMC-1 stimulated by substance P, which promotes histamine release. Columbianetin also inhibited the histamine release by substance P. In conclusion, these results indicate that Columbianetin may be helpful in regulating mast cell-mediated allergic inflammatory responses.</P>

Synergistic Effects of KH-red Ginseng/chlorella on the Endurance Capacity and Immune Enhancing in Forced Swimming Tested Mice

In-Young Choi,Ji-Hyun Lee,Tae-Hee Kang,Hyo-Jin An,Su-Jin Kim,Phil-Dong Moon,Na-Hyung Kim,Noh-Yil Myung,Deok-Chun Yang,In-Cheol Kang,Youngjin Choi,Min-Ho Kim,Jae-Young Um,Seung-Heon Hong,Hyung-Min Kim 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.6

KH-red ginseng/chlorella (KH-RG/C) is the mixed material of the Korean red ginseng powder (Panax ginseng, 75%) and extract of Chlorella vulgaris (25%). To evaluate the effects of KH-RG/C on endurance capacity and immune regulation, the forced swimming test (FST) was conducted. The immobility time in the FST was significantly decreased in KH-RG/C treated group compared with the DW-treated group at the 3 and 10 days, respectively. In the analysis of the blood biochemical parameters, KH-RG/C treatment significantly increased the glucose level. However, the lactic dehydrogenase level decreased. Although KH-RG/C increased aspartate aminotransferase, it was not different significantly. And KH-RG/C had no affects in the alanine aminotransferase, and blood urea nitrogen levels. In splenocytes and macrophages, KH-RG/C also did not affect the interleukin (IL)-2, IL-4, and IL-12 production. These results suggest that KH-RG/C may influence to immune regulation through increasing the physical endurance capacity without effect in activation of immune cells.

Effect of catalytic reactor bed dilution on product distribution for Fischer-Tropsch synthesis over Ru/Co/Al₂O₃ catalyst

Hong, Gi Hoon,Noh, Young Su,Park, Ji In,Shin, Seol A,Moon, Dong Ju Elsevier 2018 CATALYSIS TODAY - Vol.303 No.-

<P><B>Abstract</B></P> <P>The effect of catalytic reactor bed dilution on product distribution in Fischer-Tropsch synthesis (FTS) over Ru/Co/Al<SUB>2</SUB>O<SUB>3</SUB> catalysts was investigated. Ru/Co/Al<SUB>2</SUB>O<SUB>3</SUB> catalysts were prepared by a two-step impregnation method and characterized by N<SUB>2</SUB> physisorption, CO chemisorption, XRD, and TPR techniques. The catalyst bed was prepared by the layered dilution method with four different cases. The dilution of the catalyst bed affected the temperature profile and bed residence time of the catalytic reactor bed. Furthermore, CO conversion and CH<SUB>4</SUB> selectivity were affected by temperature distribution, which varied with the degree of dilution in the fixed-bed reactor. The olefin to paraffin ratio of C<SUB>2</SUB>-C<SUB>4</SUB> products and the chain growth probability (α) were influenced by the residence time of the catalytic reactor bed, which varied with the layered dilution method. A transition zone was thought to exist between the catalyst layers, which was referred to as an interval. With a long interval, the heat generated by the exothermic reaction over the catalyst layer would not be appropriately transferred from one layer to another, and the readsorption of α-olefin from pellet to pellet would also become more difficult. The dilution of the catalyst layer in the fixed-bed reactor was determined as an important parameter for optimizing product selectivity in the FTS reaction. Based on the results, the pellet to pellet readsorption model of α-olefin was proposed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Reactor bed was diluted by layered dilution method using Ru/Co/Al<SUB>2</SUB>O<SUB>3</SUB> catalyst and γ-Al<SUB>2</SUB>O<SUB>3</SUB> different 4 cases. </LI> <LI> Catalytic performance for Fischer-Tropsch synthesis reaction depends on the dilution method. </LI> <LI> Dilution of reactor bed in fixed bed reactor influenced in bed residence time in reactor bed. </LI> <LI> Readsorption of α-olefin in pellet to pellet model was suggested. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Wernicke`s encephalopathy after total parenteral nutrition in patients with Crohn`s disease

( In Seub Shin ),( Hyeri Seok ),( Yeong Hee Eun ),( You Bin Lee ),( Seung Eun Lee ),( Eun Ran Kim ),( Dong Kyung Chang ),( Young Ho Kim ),( Sung Noh Hong ) 대한장연구학회 2016 Intestinal Research Vol.14 No.2

Micronutrient deficiencies in Crohn`s disease (CD) patients are not uncommon and usually result in a combination of reduced dietary intake, disease-related malabsorption, and a catabolic state. Decreased serum thiamine levels are often reported in patients with CD. Wernicke`s encephalopathy (WE) is a severe form of thiamine deficiency that can cause serious neurologic complications. Although WE is known to occur frequently in alcoholics, a number of non-alcoholic causes have also been reported. Here, we report two cases of non-alcoholic WE that developed in two severely malnourished CD patients who were supported by prolonged total parenteral nutrition without thiamine supplementation. These patients complained of sudden-onset ophthalmopathy, cerebellar dysfunction, and confusion. Magnetic resonance imaging allowed definitive diagnosis for WE despite poor sensitivity. The intravenous administration of thiamine alleviated the symptoms of WE dramatically. We emphasize the importance of thiamine supplementation for malnourished patients even if they are not alcoholics, especially in those with CD. (Intest Res 2016;14:191-196)

Clinical and Genetic Characteristics of Korean Patients Diagnosed with Chronic Enteropathy Associated with SLCO2A1 Gene: A KASID Multicenter Study

( Hee Seung Hong ),( Jiwon Baek ),( Jae Chul Park ),( Ho-su Lee ),( Dohoon Park ),( A-ran Yoon ),( Soo Jung Park ),( Sung Noh Hong ),( Seong-joon Koh ),( Chang Kyun Lee ),( Bo-in Lee ),( Sung Wook Hwa 대한소화기기능성질환·운동학회 2022 Gut and Liver Vol.16 No.6

Background/Aims: Chronic enteropathy associated with SLCO2A1 gene (CEAS), an inherited disease characterized by nonspecific intestinal ulcers, has emerged in the Japanese population via loss-of-function mutations in the SLCO2A1 gene. We aimed to investigate the clinical and genetic characteristics of Korean patients diagnosed with CEAS. Methods: From July 2018 to July 2021, we performed Sanger sequencing of the SLCO2A1 gene in 46 patients with chronic intestinal ulcers. CEAS was confirmed based on known SLCO2A1 mutations. We summarized the clinical characteristics of patients with confirmed CEAS. Results: Fourteen out of 46 patients (30.4%) had genetically confirmed CEAS, and two SLCO2A1 variants were detected (splicing site variant c.940+1G>A and nonsense mutation [p.R603X] in SLCO2A1). Twelve patients (85.7%) were females and the median age at diagnosis of CEAS was 44.5 years. All patients presented with abdominal pain, and 13 patients (92.9%) presented with anemia (median hemoglobin, 9.6 g/dL). Ten patients (71.4%) had hypoalbuminemia (median, 2.7 g/dL). The most commonly involved site was the ileum (13/14, 92.9%). Manifestations of primary hypertrophic osteoarthropathy (PHO), such as digital clubbing, pachydermia, and periostosis were observed in five patients (28.6%) and two male patients and one female patient satisfied all major PHO diagnostic criteria. Conclusions: The clinical and genetic characteristics of Korean patients with confirmed CEAS were similar to those reported in the literature. CEAS should be considered in the differential diagnosis for patients with unexplained chronic nonspecific ulcers of the small intestine. (Gut Liver 2022;16:942-951)

국내 대장 폴립 절제 후 추적검사 현황

홍성노 ( Sung Noh Hong ),양동훈 ( Dong Hoon Yang ),김영호 ( Young Ho Kim ),김성은 ( Seong Eun Kim ),신성재 ( Sung Jae Shin ),홍성필 ( Sung Pil Hong ),이보인 ( Bo In Lee ),이석호 ( Suck Ho Lee ),박동일 ( Dong Il Park ),김현수 ( Hyu 대한장연구학회 2011 Intestinal Research Vol.9 No.2

Background/Aims: There is a paucity of information on postpolypectomy surveillance currently practiced in Korea. Thus, we investigated the present state of postpolypectomy surveillance in Korea using a web-based survey. Methods:A multiple choice questionnaire was used to determine the preferred surveillance modality, the colonoscopic surveillance interval used in 11 case scenarios, and clinical factors influencing surveillance intervals. The form was sent via e-mail to members of the Korean Association for the Study of Intestinal Diseases and primary care physicians involved in a colonoscopy surveillance program. Of 425 colonoscopists contacted, 263 replied (response rate, 62%). Of the respondents, 94% were internists and 54% practiced in tertiary referral hospitals. Results: All respondents chose colonoscopy as a preferred surveillance modality following polyp removal. Colonoscopy at 3 years was the most frequent answer after removal of 1 or 2 tubular adenoma(s) <1 cm in size, while 1 year was the most frequent answer after removal of an advanced adenoma or ≥3 adenomas, and 6 months was the most frequent choice after removal of adenoma with high-grade dysplasia or a sessile polyp ≥2 cm. The agreement rate for the time of first surveillance between preferred guideline recommendations and respondent answers was in the low range at 14-43%. Conclusions: A significant disagreement exists between current postpolypectomy surveillance practices of Korean colonoscopists and preferred guideline recommendations. This discrepancy may be due to the fact that the guidelines do not reflect recent studies and the specific medical environment in Korea. Thus, there is a need to develop new evidence-based Korean guidelines for postpolypectomy surveillance. (Intest Res 2011;9:118-128)

Characterization of Mycoplasma Arginine Deiminase Expressed in E. coli and Its Inhibitory Regulation of Nitric Oxide Synthesis

Eun Joo Noh,Sang Wook Kang,신용재,Dong Chung Kim,In Sun Park,Min Young Kim,Boe Gwun Chun,Bon Hong Min 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.1

We previously reported that a cytostatic protein that is found in ASC-17D Sertoli cell-conditioned media was Mycoplasma arginine deiminase (ADI), which hydrolyzes L-arginine into L-citrulline and ammonia. Here, we report the over-expression of recombinant ADI (rADI) in E. coli and the down-regulation of lipopolysaccharide (LPS) induced-nitric oxide (NO) production by rADI treatment. We cloned the ADI gene from Mycoplasma arginini genomic DNA by a polymerase chain reaction, and changed five TGA tryptophan codons (stop codon in E. coli) to TGG codons in the coding region by site-directed mutagenesis in order to express in E. coli. The rADI was purified to apparent homogeneity by DEAE-Sepharose and arginine-affinity chromatography. The rADI expressed in E. coli was identified as 45 kDa on SDS-PAGE and 90 kDa on native PAGE, implying that it exists as a dimer like ADI of M. arginini. The Km for arginine of rADI was approximately 370 ± 50 mM. Its optimal temperature and pH were 41oC and pH 6.4, respectively, and enzyme activity remained ³ 50% for 5 d at physiological temperature and pH. Treatment of purified rADI suppressed NO production in macrophage-like RAW 264.7 and primary glial cells that were exposed to LPS. Furthermore, an intraperitoneal injection of rADI significantly suppressed the rise of blood nitrite/nitrate levels that were induced by the systemic administration of bacterial endotoxin LPS to mice, resulting in an improvement in their survival rate. These results suggest that the depletion of blood arginine with an arginine- metabolizing enzyme, such as ADI, could suppress excessive production of NO that is caused by inducible NOS (iNOS) during the endotoxemia. Also, rADI may be used as a new approach to control NO-related diseases, such as sepsis.