강산 및 강알칼리 음독에서 냉각 용액을 사용한 희석 요법과 중화 요법의 가능성

이중의,송형곤,김동훈,권운용,곽영호,서길준,윤여규 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: There is no effective treatment modality for caustic agent ingestion. Dilution and neutralization are prohibited because of the risk of secondary thermal injury. This experiment is designed to evaluate the amount of dilution and neutralization heat and to gauge the applicability of dilution and neutralization therapy using cold solutions to suppress the peak temperature. Methods: This is an in-vitro chemical experiment. HCl, CH3COOH, NaOH, and NH4OH are selected as representatives of strong and weak acids and strong and weak alkali, respectively. 20℃, 11.6M, 5.8M, and 2.9M solutions of each acid and alkali are made and mixed using a magnetic stirrer at a room air temperature of 28℃. The peak temperature, the duration above 40℃, and the heat amount are measured or calculated. Results: When a 11.6M HCl or NaOH solution is diluted with same amount of water, 32 or 18cal. per mL of HCl or NaOH is produced, respectively. HCl produces a significant peak temperature, but NaOH does not. The lower the concentration, the lower the amount of heat production. 11.6M CH3COOH and NH4OH solutions don't produce dilution heat.11.6M and 5.8M solutions of all acids and alkali produce destructive neutralization heat. However, 2.9M solutions produce neutralization heat which might be controllable. When a 11.6M HCl or NaOH solution is neutralized with a -10℃ 2.9 M NaOH or HCl solution, respectively, the peak temperature produced is below 40℃ and seems to add little thermal damage to viable tissue. Conclusion: Dilution and neutralization with a cold solution in cases of strong acid or alkali ingestion is a promising method to avoid thermal injury.

2진 FSK 시스템에서 구속장이 7인 (2,1) Viterbi 복호기를 갖는 광헤테로다인 수신기의 성능 분석

홍영주(Young-Ju Hong),한종석(Jong-Seok Han),홍권의(Kweon-Eui Hong),정진호(Jin-Ho Jung),김영권(Yung-Kwon Kim) 대한전자공학회 1993 대한전자공학회 학술대회 Vol.1993 No.11

Flavimarina flava sp. nov., isolated from Salicornia herbacea

Cho, Eui-Sang,Cha, In-Tae,Park, Jung-Min,Choi, Hak-Jong,Lee, Jong Hun,Roh, Seong Woon,Cho, Eun-Ah,Kweon, Mee-Hyang,Nam, Young-Do,Seo, Myung-Ji Microbiology Society 2017 International journal of systematic and evolutiona Vol.67 No.10

<P>A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091(T). It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73(T) with 94.8% similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F. pacifica KCTC 32466(T) and Leeuwenhoekiella marinoflava DSM 3653(T) were 80.5 and 80.2 %, respectively. Growth of strain MBLN091(T) was observed in the presence of 0.5-15.0% (w/v) NaCl at 4-35 degrees C and pH 6.0-8.0, with optimal growth in the presence of 2.5-5.0% (w/v) NaCl at 20-25 degrees C and pH 7.0. This isolate was able to hydrolyse gelatin. The only respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. Major fatty acids of the isolate were iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-17:0 3-OH and iso-C-15:1 G. The genomic DNA G+C content was 39.6 mol%. The physiological features were closely related to F. pacifica. Therefore, strain MBLN091(T) is considered to represent a novel species within the genus Flavimarina, for which the name Flavimarina flava sp. nov. is proposed. The type strain is MBLN091(T) (=KCTC 52527(T)=JCM 31731(T)).</P>

Expression of Neurotrophic Factors and their Receptors in Keratoconic Cornea

Chung, Eui Sang,Lee, Kwang Hyun,Kim, Min,Chang, Eun Ju,Chung, Tae Young,Kim, Eung Kweon,Lee, Hyung Keun Informa Healthcare USA, Inc. 2013 Current eye research Vol.38 No.7

<P><I>Purpose</I>: To measure the expression level and pattern of neurotrophic factors and their receptors in keratoconus (KC) cornea using quantitative RT-PCR (qPCR) and immunostaining.</P><P><I>Materials and Methods</I>: Twenty-one recipient corneal buttons after keratoplasty from KC cornea and four age-matched normal corneas were obtained. The 25 corneal tissues were divided into two pieces; one fragment of each sample was used for immunostaining whereas the other fragment was used for qPCR. Using primary antibodies and specific qPCR primer, immunostaining and qPCR were performed to analyze the expression level of the neurotrophic factors and receptors.</P><P><I>Results</I>: Nerve growth factor and its receptors (TrkA and p75NTR) were not expressed in the center of normal cornea. However, TrkA and the p75NTR were clearly expressed in a membrane bound staining pattern and the mRNA levels were significantly higher in KC (<I>p</I> < 0.001). The mRNA expression of brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and neurotrophin (NT)-4 mRNA was also elevated in KC (<I>p</I> < 0.001). BDNF was expressed in epithelium of normal cornea. However, in KC, its expression was found to be extending into the anterior stromal layer. CNTF was hardly expressed in normal cornea. In KC, the entire epithelium expressed CNTF, especially in perinuclear area. NT-4 was expressed throughout the epithelium and stroma in KC.</P><P><I>Conclusion</I>: The change in expression of neurotrophic factors in KC may suggest that these factors play an important role in the pathogenesis of KC and serve as new markers for the progression of KC.</P>

Identification of gene targets eliciting improved alcohol tolerance in <i>Saccharomyces cerevisiae</i> through inverse metabolic engineering

Hong, Min-Eui,Lee, Ki-Sung,Yu, Byung Jo,Sung, Young-Je,Park, Sung Min,Koo, Hyun Min,Kweon, Dae-Hyuk,Park, Jae Chan,Jin, Yong-Su Elsevier 2010 Journal of biotechnology Vol.149 No.1

<P><B>Abstract</B></P><P>The economic production of biofuels from renewable biomass using <I>Saccharomyces cerevisiae</I> requires tolerance to high concentrations of sugar and alcohol. Here we applied an inverse metabolic engineering approach to identify endogenous gene targets conferring improved alcohol tolerance in <I>S. cerevisiae</I>. After transformation with a <I>S. cerevisiae</I> genomic library, enrichment of the transformants exhibiting improved tolerance was performed by serial subculture in the presence of iso-butanol (1%). Through sequence analysis of the isolated plasmids from the selected transformants, four endogenous <I>S. cerevisiae</I> genes were identified as overexpression targets eliciting improved tolerance to both iso-butanol and ethanol. Overexpression of <I>INO1</I>, <I>DOG1</I>, <I>HAL1</I> or a truncated form of <I>MSN2</I> resulted in remarkably increased tolerance to high concentrations of iso-butanol and ethanol. Overexpression of <I>INO1</I> elicited the highest ethanol tolerance, resulting in higher titers and volumetric productivities in the fermentation experiments performed with high glucose concentrations. In addition, the <I>INO1</I>-overexpressing strain showed a threefold increase in the specific growth rate as compared to that of the control strain under conditions of high levels of glucose (10%) and ethanol (5%). Although alcohol tolerance in yeast is a complex trait affected by simultaneous interactions of many genes, our results using a genomic library reveal potential target genes for better understanding and possible engineering of metabolic pathways underlying alcohol tolerance phenotypes.</P>

Enhancement of the anisotropic photocurrent in ferroelectric oxides by strain gradients

Chu, Kanghyun,Jang, Byung-Kweon,Sung, Ji Ho,Shin, Yoon Ah,Lee, Eui-Sup,Song, Kyung,Lee, Jin Hong,Woo, Chang-Su,Kim, Seung Jin,Choi, Si-Young,Koo, Tae Yeong,Kim, Yong-Hyun,Oh, Sang-Ho,Jo, Moon-Ho,Yang, Nature Publishing Group 2015 Nature nanotechnology Vol.10 No.11

The phase separation of multiple competing structural/ferroelectric phases has attracted particular attention owing to its excellent electromechanical properties. Little is known, however, about the strain-gradient-induced electronic phenomena at the interface of competing structural phases. Here, we investigate the polymorphic phase interface of bismuth ferrites using spatially resolved photocurrent measurements, present the observation of a large enhancement of the anisotropic interfacial photocurrent by two orders of magnitude, and discuss the possible mechanism on the basis of the flexoelectric effect. Nanoscale characterizations of the photosensitive area through position-sensitive angle-resolved piezoresponse force microscopy and electron holography techniques, in conjunction with phase field simulation, reveal that regularly ordered dipole-charged domain walls emerge. These findings offer practical implications for complex oxide optoelectronics.

Ni 본드코팅이 Al 기지에 고온 용사 코팅된 Fe 코팅층의 접합특성에 미치는 영향

권의표,김대영,이종권,Kwon, Eui-Pyo,Kim, Dae-Young,Lee, Jong-Kweon 한국재료학회 2016 한국재료학회지 Vol.26 No.10

The influence of NiCrAlY bond coating on the adhesion properties of an Fe thermal coating sprayed on an Al substrate was investigated. By applying a bond coat, an adhesion strength of 21MPa was obtained, which was higher than the 15.5MPa strength of the coating without the bond coat. Formation of cracks at the interface of the bond coat and the Al substrate was suppressed by applying the bond coat. Microstructural analysis of the coating interface using EBSD and TEM indicated that the dominant bonding mechanism was mechanical interlocking. Mechanical interlocking without crack defects in the coating interface may improve the adhesion strength of the coating. In conclusion, the use of an NiCrAlY bond coat is an effective method of improving the adhesion properties of thermal sprayed Fe coatings on Al substrates.

SIRT1 activation ameliorates inflammation in the outer root sheath cells of hair follicles

( Jin Park ),( Lihua Hao ),( Eui-sung Jung ),( Seok-kweon Yun ),( Han-uk Kim ),( Young Lee ),( Chang-deok Kim ),( Byung-hyun Park ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Alopecia areata (AA) is T-cell-mediated autoimmune disease. Type III histone deacetylase silent information regulator 1 (SIRT1) is kown to be involved in several autoimmune inflammatory disease including psoriasis, vitiligo. However, little is known about SIRT1 activation in AA. Objectives: We investigated the role of SIRT1 activation in the outer root sheath (ORS) cells of hair follicles. Methods: Sirt1 expression of scalp tissue of 23 AA patients was evaluated by immunohistochemical staining, polymerase chain reaction (PCR) and western blot analysis. Cultured human ORS were pretreated with Sirt1 activator and inhibitor, and then stimulated with polyinosinic:polycytidylic acid (poly(I:C)). Results: SIRT1 level was significantly decreased in AA patients than in healthy controls. The activation of inflammatory chemokines (CXCL3, CXCL9, CXCL10) and cytokines (IL-1, IL-6, IFN-γ) were downregulated by pharmacologic activation of SIRT1, while SIRT1 inhibitors upregulated poly(I:C) induced cytokine and chemokine production. Mechanistically, this poly(I:C) induced inflammation by SIRT1 inhibition was associated with activation of NF-κB signaling pathway in ORS cells. Finally, we found that T cell migrations study using chemoattractant-conditioned media also downregulated by SIRT1 activation. Conclusion: SIRT1 plays a significant role in the pathogenesis of AA and can be a potential therapeutic target for the treatment of AA.

Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering

Ki-Sung LEE,Min-Eui HONG,Seok-Chae JUNG,Young-Je SUNG,Dae-Hyuk KWEON,Yong-Su JIN 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

조산의 발생 빈도 및 원인 변화에 관한 임상적 고찰

정연욱 ( Yeon Uk Jung ),권오준 ( Oh Joon Kweon ),한용보 ( Yong Bo Han ),김금석 ( Kum Seok Kim ),이광범 ( Kwang Bum Lee ),김석영 ( Suk Young Kim ),이의돈 ( Eui Don Lee ) 대한주산의학회 2002 Perinatology Vol.13 No.2

연구목적:신생아 사망률과 이환율의 가장 큰 원인인 조산의 발생 빈도 및 원인 변화를 1995년과 2000년의 조산아 분만을 통해 비교해 보고자 하였다. 방법:가천의대 부속 길 병원에서 1995년 1월 1일부터 8월 31일까지 분만 총 5309례 중 20주 이상 37주미만의 조산 438례와 2000년 1월 1일부터 8월 31일까지 분만 총 2028례 중 20주 이상 37주미만의 조산 319례를 대상으로 연령 분포, 분만력, 분만 방법, 신생아의 성, 분만 시 체중 등을 조사하였다. 결과:전체 분만 당 조산의 비율은 1995년 8.1%에서 2000년 15.7%로 증가 하였고, 조산의 원인으로는 원인 불명은 25.5%(109예)에서 20.2%(66예)로, 조기 양막파수는 26.9%(115예)에서 22.9%(73예)로, 자궁경관무력증은 9.7%(41명)에서 6.9%(22예)로, 기타 원인 8.9%(38예)에서 5.1%(16예)로, 자궁기형은 1.6%(7예)에서 1.0%(3예)로 감소 소견을 보였으나 기타 원인인 산모의 질병 및 내과적 문제에서만 통계학적으로 유의하였고, 다태임신은 10.1%(44예)에서 17.6%(56예)로, 태아기형은 2.1%(9예)에서 6.3%(20예)로, 임신성 고혈압증은 7.9%(34예)에서 13.3%(42예)로 유의하게 증가하였고, 전치태반이나 태반 조기박리 같은 태반의 이상이 7.3%(31예)에서 6.7%(21예)로 큰 변화가 없었다. 결론:본 연구에서 1995년과 비교하여 2000년 조산 자료에서는 원인 불명의 수가 감소한 것은 이전보다 좀더 조산의 원인에 대한 파악이 이루어진 것으로 추측되며, 다태임신의 증가는 최근 불임 시술의 증가에 따른 것으로 여겨지며 조산의 원인으로 태아기형의 증가는 약물 남용, 환경 오염 문제 및 산전 진단의 발달과 관련 있는 것으로 생각된다. 임신성 고혈압증의 증가는 이전의 다른 연구들에서 감소를 보인 것과는 달리 증가를 보인 것은 전체적인 조산비율의 증가와 함께 개인 병원에서의 3차 병원으로의 전원의 증가로 여겨진다. 지속적으로 좀더 기간을 두고 조산 원인에 대한 비교와 파악이 이루어져 이를 통해 신생아 사망률과 이환율 감소에 기초 자료로 활용될 수 있기를 기대한다. Objective:The aim of study was to compare incidence rate and causes of the preterm birth that are the major factor of newborn mortality and morbidity. Methods:Respectively there were 428 preterm deliveries out of 5,309 deliveries, from January to August 1995, and there were 319 preterm deliveries out of 2,028 deliveries, from January to August 2000 at Gill hospital, a hospital in affiliation with Gachon Medical School. The data were collected by review of the hospital record and the statistical analysis was performed using paired T-tests, Oneway ANOVA, Fisher`s exact test, and statistics significance was defined as p<0.05. Results:The incidence rate of the preterm birth increased from 8.1%(428/5,309) in 1995 to 15.7%(319/2,028) in 2000. Unknown causes of preterm birth decreased from 25.5%(109) to 20.2%(66). PPROM(Preterm premature rupture of the membranes) decreased from 26.9%(115) to 22.9%(73). IIOC(Incompetent internal os of cervix) decreased from 9.7%(41) to 6.9%(22). Uterine anomaly decreased from 1.6%(7) to 1.0%(3). And other causes deceased from 8.9%(38) to 5.1%(16). Multiple pregnancy increased from 10.1%(44) to 17.6%(56). Fetal anomaly increased from 2.1%(9) to 6.3%(20). Pregnancy induced hypertension increased from 7.9%(34) to 13.3%(42). There was not much change for the placental disorder or for placental abruption which decreased from 7.3%(31) to 6.7%(21). According to the analysis, there is a noticeable decrement in IIOC but noticeable increase in multiple pregnancy, fetal anomaly and PIH. Conclusion:Comparing the data from the preterm birth of 1995 and 2000, the unknown cause of the preterm birth is understood more accordingly to the decrement of known cause. The increase of multiple pregnancy seems to be caused by better in vitro fertilization. The increase of fetal anomaly is most likely caused by drug abuse, environmental pollution, and prenatal diagnosis. Differ from the decrease of previous research, the increase of PIH is caused by the increase of incidence of the preterm birth and also patients moving from private hospital to 3rd party hospital. We hope that we can continuously research the cause of the preterm birth and use that basic information to decrease the death and disease rates of newborn infants.