각종 피부종양에서 p53과 bcl-2 발현양상

정용민,양승하,이성열,김대중,김의한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

Background : The function of p53 protein was known to accelerate the apoptosis, but bcl-2 protein was known to suppress the apoptosis. The overexpression of p53 and bcl-2 proteins has been reported in many malignant tumors. Although the mechanism of tumorgenesis of the p53 and bcl-2 protein took hasnot been proved, bcl-2 positive tumors contrast to p53, appeared to have good prognosis. The expression pattern of p53 and bcl-2 in epidermal tumors has not been well known. The author analyzed immunohistochemical expression of p53 and bcl-2 protein and investigated the role of p53 and bcl-2 protein in the developing tumors of epidermal origin. Method : 15 cases of squamous cell carcinoma, 20 cases of basal cell carcinoma, 10 cases of keratoacanthoma and 6 cases of Bowen's disease were stained immunohistochemically stain with anti p53 and anti bcl-2 monoclonal antibody. Results : 1. In squamous cell carcinoma, the reaction of p53 was positive in 13 cases(86%) and the stain pattern showed more intensive reaction in poorly differentiated cells in the same tumor. bcl-2 showed negative in all cases of squamous cell carcinoma(0%). 2. In basal cell carcinoma, the reaction of p53 was positive in 10 cases (50%). The positive cells were mainly observed in squamous metaplastic portion, but the reaction was weak. bcl-2 showed positive in all cases of basal cell carcinoma (100%) and the reaction was strong. 3. In keratoacanthoma, p53 was positive in 7 cases (70%). The positive cells were mainly observed in the cells around the basement membrane. This finding suggested that p53 positive basal cells were programmed to become keratoacanthoma. bcl-2 was negative in all 10 cases of keratoacanthoma. (0%) In some cases, bcl-2 positive cells were observed around the basement membrane in some cases., which were negative to p53 This finding suggested that the existence of these bcl-2 positive cells were related to the prognosis of keratoacanthoma. 4. In Bowen's disease, p53 was positive in 5 cases ( 83% ) and the reaction was strong, so the tumor cells in Bowen's disease appeared to be poorly differentiated type. bcl-2 was negative in all 6 cases ( 0% ) of Bowen's disease, but bcl-2 positive cells were observed in the basement membrane in some cases.

만선신부전 환자에서 관상동맥조영술 소견에 관한 연구

한대희,김성구,박상호,김성한,조원영,방덕원,조윤행,정의룡,은영근,권영구 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Patients with chronic renal fialure have a substantially elevated risk of death from cardiovascular diseases than do the general population. the patients with chronic renal failure are at significantly increased freqeuncy of hypertension, hyperlipidemia, and diabetes mellitus which are known to the risk factors of the coronary artery diseases, and the prevalence of the coronary artery diseases in chronic renal failure patients is highly associated with the hemodynamic disorder and metabolic abnormalities. therefore we expected that the coronary angiographic findings in patients with chronic renal failure should be different from the general population (control groups) and investigated the risk factors contributing to coronary artery diseases. Method : we have retrospectively compared the coronary angiographic findings of 44 patients with chronic renal failure on hemodialysis with that of 88 patients in the general population and investigated the factors contributing to the development and acceleration of coronary artery diseases in patients with chronic renal failure Result : Hypertension and diabetes mellitus which is risk factors for coronary artery disease is significantly increased in patients with chronic renal failure, in coronary angiographic finding the severity of the lesion is worse. the incidence of PCI or CABG of patients with chronic renal failure is more than that of control group but statistically no difference between patients with chronic renal failure and control group. the sex, the duration of disease, the duration of dialysis, serum creatinine in patients with chronic renal failure have no correlation to the prevalence of coronary artery disease and severity of lesion. hyperglycemic patients with chronic renal failure have high incidence of coronary artery disease and are worse in the severity of lesion Conclusions : There is significantly increased the pevalence and severity of involving multiple coronary artery diseses in hyperglycemic patients with CRF.

전산화 신경인지 검사를 이용한 외상후 스트레스장애 환자의 인지기능 측성

한상우,최의정,한선호 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

objective: Among the patient who injured by traffic accident, they complaint decreased cognitive functioning from severe traumatic brain injury and mild traumatic brain injury such as 'post-concussional syndrome' to 'compensation neurosis' But Physicions ignore the evaluation and treatment of the patient with mild traumatic brain injury and no head trauma history who complaint cognitive disturbance, because they are tend to considered as malingering or 'compensation neurosis'. In this study, the authors evaluated cognitive functioning of the patient with post-traumatic stress disorder who were admitted by traffic accident and were no evidence of head trauma. method : Among the thirty patients who were admitted by traffic accident in Soonchunhyang university hospital, authors selected the patients that 1) had no evidence of head trauma, and 2) have no prominent faking-bad attitude, and 3)have the symtomatology of posttraumatic stress disorder. The number of the selected patient was eighteen, and authors performed physical and psychiatric examination including computed neuropsychological test. results : the result of computed neuropsychological test, thirteen patients had mild attention impariment and, six patients had impairment of immediate recalling, ten patients had mild visuoperceptual disablilty. conclusions : In this study, some patients with posttraumatic stress disorder had impaired attention, immediate recalling, visuoperceptual functioning in computed neuopsychological test.

잉어 담즙이 간장에 미치는 영향에 대한 연구

김대중,양승하,안상묵,강상균,김의한 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

To investigate the effect of Cyprinus carpio bile acid on human liver, concentrated and 10:1 diluted Cyprinus carpio bile acid were injected at the dose of 10ul at mouse tail vein One, three, five, seven and nine days after injection, the mice were sarcificed. The change in hepatic function were estimated by determining GOT, GPT and compared to histologic findings of liver tissue. The results of GOT level was not consistent, but the GPT was significant. From these, we concluded. 1. In concentrated injected group (A), the GPT level was highest in first day and the increased level lasted through observation. 2. In 10:1 diluted injected group (B), the GPT level was only high above normal in first day. Thereafter, the GPT level curiously decreased below the normal, which need more study. 3. Histologic hepatic changes in A group, until 3 days, hepatic cell cord disarrary and marked hepatic cellular degeneration around the central vein were seen. Therefater, although elevated GPT level, the hepatic changes showed only mild regenerated change Histologic hepatic change in B group showed no remarkable change. Above findings suggested that the liver injury was dependent on injected concentration and the hepatic injury, which was observed in early phase, appeared to be reversible. So the hepatic injury by the Cyprinus carpio bile acid appeared complete recovery course without specific complication.

폐장암에서 p53과 PCNA 표출에 관한 연구

김대중,양승하,정도영,강상균,김의한 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

To observe the expression of p53 protein and PCNA in lung carcinomas and to evaluate the correlationship between the two results, the author has been carried out immunohistochemical staining with mononuclear antibodies against p53 and PCNA. 64 cases of lung carcinomas used consist of 32 cases of squamous cell carcinomas, 18 cases of adenocarcinomas and 14 cases of small cell carcinomas, respectively. 1. p53 protein expression was seen in 16 cases out of 32 (50%) in squamous cell carcinoma, 7 cases out of 18 (39%) in adenocarcinoma and 10 cases out of 14 (71%) in small cell carcinoma. The positive rate of p53 is highest in small cell carcinoma. 2. p53 staining degrees showed that 3+ in 75% of squamous cell carcinoma, 1+ in 58% of adenocarcinoma and 1+ in 50% in small cell carcinma. were observed. The tumor cell composing of squamous cell carcinoma showed most strong staining to p53 protien. 3. PCNA labelling index showed higher index in p53(+) cases than p53(-) cases. The PCNA index of squamous cell carcinoma was highest and adenocarcinoma and small cell carcinoma in order. Above findings suggested that the PCNA index was related to p53 protein in tumor cells, but the PCNA index appeared to be influenced by the p53 protein content in tumor cells than whether the p53 protien was or not. So the growth rate of squamous cell carcinoma appeared to be most rapid than adenocarcinoma and small cell carcinoma.

유방암에서 bcl-2와 p53 표출에 관한 연구

김의한,김대중,강상균,양승하,홍기언 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

In order to observe the expression of bcl-2 and p53 protein in the 29 cases of invasive ductal carcinomas and to evaluate the correlationship between the two results, the author has carried out immunohistochemical staining with mononuclear antibodies against bcl-2 and p53. The results are obtained as follows: 1. bc1-2 showed positive reaction in normal duct, lobule and hyperplastic epithelium, and p53 showed no reaction in the same tissue. 2. In 15 cases of well differentiated type, the bc1-2 was positive in 14 cases (93%), and the p53 was positive only in 4 cases (26%). In 6 poorly differentiated type, the bcl-2 was positive in 2 cases (33%), and the p53 was positive in 3 cases (50%). The relationship between the bcl-2 and the p53 showed that in well differentiated type, bcl-2(+)/p53(-) expression was most commonly observed, while the bcl-2(-)/p53(+) expression was not seen in well differentiated type. 3. The size of tumor appeared to have no direct relationship to bcl-2 and p53 positive rate. 4. The expression of bcl-2 and p53 in intraductal carcinoma portion showed that when intraductal tumor component was positive, the invasive portion tumor cell was also positive. However the bcl-2 positive tumor cells in some intraductal component apeared to be negative in p53. Above findings showed that the bcl-2 protein was more frequently expressed in low grade lesion and the p53 protein was in high grade lesion. Also bcl-2 and p53 positive or negative findings showed different results in the same tumor cells.

사무직 근로자에서 직무스트레스 및 VDT작업과 목·어깨 근골격계 증상과의 관련성

이의철,김환철,정달영,김동현,임종한,박신구 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.3

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

삼백초(Saururus Chinensis (Lour.) Bail) 열수추출물의 암세포 저해 효과 및 항산화효소 활성에 미치는 영향

양의주,한정,김현정,이인선 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

삼백초(Saururus Chinensis (Lour.) Bail)열수추출물의 항암활성 검색을 위하여 여러 암세포에 대한 저해효과와 항산화효과를 측정하고 발암물질인 MNNG를 투여한 동물조직내의 항산화 효소 활성변화에 미치는 영향 등을 살펴보았다. 그 결과 삼백초 열수추출물은 특히 혈액암 세포인 U937에 대해 가장 높은 저해 활성을 나타내었고, 또한 Chinese hamster V79 cell을 이용한 H2O2로 유도된 세포독성에 대한 억제효과도 나타내었다. 그리고 삼백초 열수추출물은 MNNG를 투여한 다음 감소된 glutathione S-transferase와 superoxide dismutase의 활성과 glutathion의 함량을 높여주었다. 이상의 결과로 삼백초 열수추출물은 항산화에 관련하여 발암을 예방하리라 사료된다. Chemoprevetion effects of Saururus Chinensis (Lour.) Bail water extracts on several tumor cells, Chinese hamster V79 cells and antioxidant enzymes in Wistar male rats were investigated. The water extracts of Saururus Chinensis (Lour.) Bail showed a higher cytotoxicity effect on the human histiocytic leukemia cells(U937) and protective effects against the cytotoxicity of H2O2. The glutathione content was decreased by MNNG treatment but was increased by adding Saururus Chinensis (Lour.) Bail water extracts. Also the activities of glutathione S-transferase and superoxide dismutase were increased by the treatment of Saururus Chinensis (Lour.) Bail water extracts more than with MNNG alone. These results suggest that the water extracts of Saururus Chinensis (Lour.) Bail inhibit the growth on U937 and has a protective effect on liver and stomach damage by the activities of free radical generating enzymes.

상피세포 종양에서 bcl-2 표출에 관한 연구

김의한,정현,이종석,이성열,조명구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Background : The bc1-2 protein, contrast to p53, has been shown to be suppress the apoptosis, and overexpression of the bc1-2 protein has been reported in several malignant tumors. Although how the bc1-2 protein takes part in tumorgenesis has not been proved, the bc1-2 positive tumors appeared to have good prognosis in some malignant tumors. The expression pattern of the bc1-2 in keratinocyte originated skin tumors have not been well known. The authors analyzed immunohistochemically expression of the bc1-2 protein to invastigate the role of the bc1-2 protein in developing skin tumors. Method : 21 squamous cell carcinoma, 19 basal cell carcinoma, 9 keratoacanthoma 11 seborrehic keratosis and 9 Bowen's disease were immunostained with anti-bc1-2 monoclonal antibody. Results : 1. The bc1-2 negative reactions were seen in all cases of sqaumous cell carcinoma, keratoacanthoma and Bowen's disease. The basal layer in keratoacanthoma and Bowen's disease shown to be intense positive reaction in bc1-2, whch suggested of the protected barrier function from tumor cell invasion into the dermis. 2. The bc1-2 were positive in all basal cell carcinoma. 3. In seborreheic keratosis, 9 cases were negative and 2 cases were positive. The positive cases appeared to be resulted from invagination of the basal cells in the epidermis. Conclusion : The bc1-2 protein did not appear to express in full matures cell, but positive reaction are seen in proliferating activity cells. The bc1-2 positive cells are rahter not abnormal cells than have function of protection of tumor cell proliferation by the prolongation of survival time normal cell.