RTN4 3'-UTR Insertion/Deletion Polymorphism and Susceptibility to Non-Small Cell Lung Cancer in Chinese Han Population

Lu, De-Yi,Mao, Xu-Hua,Zhou, Ying-Hui,Yan, Xiao-Long,Wang, Wei-Ping,Zheng, Ya-Biao,Xiao, Juan-Juan,Zhang, Ping,Wang, Jian-Guo,Ashwani, Neetika,Ding, Wei-Liang,Jiang, Hua,Shang, Yan,Wang, Ming-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Nogo protein, encoded by gene reticulon-4 (RTN4), includes three major isoforms by different splicing, named Nogo-A Nogo-B and Nogo-C. Nogo proteins play an important role in the apoptosis of cells, especially in tumor cells. RTN4 single nucleotide polymorphisms (SNPs) can influence the efficiency of transcription and translation thus being related with an individual's predisposition to cancer. The CAA insertion/deletion polymorphism (rs34917480) within RTN4 3'-UTR has been reported to be associated with many cancer types. In order to investigate the relationship between this polymorphism and susceptibility to non-small cell lung cancer (NSCLC) in the Chinese population, we conducted the present case-control study including 411 NSCLC patients and 471 unrelated healthy controls. The genotype distributions were significantly different between cases and controls (p=0.014). We found that the del allele could significantly increase NSCLC risk (ins/ins vs ins/del: p=0.007, OR 1.46, 95%CI=1.11-1.93; dominant model: p=0.004, OR 1.47, 95%CI=1.13-1.92 and allele model: p=0.008, OR 1.35, 95%CI=1.08-1.67). This association was stronger in participants over 60 years old, males and smokers. We therefore conclude that the CAA insertion/deletion polymorphism (rs34917480) contributes to non-small cell lung cancer risk in Chinese population. Age, sex and environmental exposure are also related to carcinogenic effects of rs34917480.

Decorin: a potential therapeutic candidate for ligamentum flavum hypertrophy by antagonizing TGF-β1

Wang Shanxi,Qu Yunkun,Fang Xuan,Ding Qing,Zhao Hongqi,Yu Xiaojun,Xu Tao,Lu Rui,Jing Shaoze,Liu Chaoxu,Wu Hua,Liu Yang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Ligamentum flavum hypertrophy (LFH) is the main physiological and pathological mechanism of lumbar spinal canal stenosis (LSCS). The specific mechanism for LFH has not been completely clarified. In this study, bioinformatic analysis, human ligamentum flavum (LF) tissues collection and analysis, and in vitro and in vivo experiments were conducted to explore the effect of decorin (DCN) on LFH pathogenesis. Here, we found that TGF-β1, collagen I, collagen III, α-SMA and fibronectin were significantly upregulated in hypertrophic LF samples. The DCN protein expression in hypertrophic LF samples was higher than that in non-LFH samples, but the difference was not significant. DCN inhibited the expression of TGF-β1-induced fibrosis-associated proteins in human LF cells, including collagen I, collagen III, α-SMA, and fibronectin. ELISAs showed that TGF-β1 can upregulate PINP and PIIINP in the cell supernatant, and this effect was inhibited after DCN administration. Mechanistic studies revealed that DCN suppressed TGF-β1-induced fibrosis by blocking the TGF-β1/SMAD3 signaling pathway. In addition, DCN ameliorated mechanical stress-induced LFH in vivo. In summary, our findings indicated that DCN ameliorated mechanical stress-induced LFH by antagonizing the TGF-β1/SMAD3 signaling pathway in vitro and in vivo. These findings imply that DCN is a potential therapeutic candidate for ligamentum flavum hypertrophy.

Low-Cost SVM-DTC Strategy of Induction Machine Drives Using Single DC-link Current Sensor

Wang, Wei,Cheng, Ming,Hua, Wei,Ding, Shichuan,Zhu, Ying,Zhao, Wenxiang Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.3

In conventional direct torque control (DTC) using space-vector modulation (SVM) of induction machine (IM) drives, at least three current sensors are needed. In this paper, a low-cost SVM-DTC strategy is proposed, in which only a single current sensor is used. The position of the voltage space vector is divided into two areas: effective and non-effective area. If it is located in the non-effective area, the voltage space vector will be shifted into the effective area with minimum distortion. Further, the switching frequency remains constant. The simulation is carried out on a MATLAB/Simulink platform and the simulated results verify the effectiveness of the proposed strategy.

Experimental Study on Shear Behavior of BFRP-reinforced Recycled Aggregate Concrete Deep Beams without Stirrups

Hua-xin Liu,Jian-Wei Yang,Xue-zhi Wang,Ding-Jie Han 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.6

This paper describes an experimental investigation to evaluate the shear performance of basalt Fiber-reinforced Polymer (BFRP)- reinforced recycled aggregate concrete deep beams without stirrups. A total of 13 beams were tested under four-point loading until failure: nine were reinforced with BFRP bars, and four were reinforced with steel bars. The parameters of this experiment included the shear-span-to-depth ratio (a/d), reinforcement ratio of the longitudinal bar (ρ), sectional effective depth (d) and compressive strength of the recycled concrete ( ). The shear behavior of the beams was described in terms of the crack type, failure mode, shear strength and load-deformation relationship. The effects of the experimental variables on shear capacities and deflections of the tested beams were also investigated. The experimental results showed that the shear capacity of BFRP-reinforced beams was smaller than that of steel-reinforced beams. Additionally, the deformation of BFRP-reinforced beams was larger than that of steel-reinforced ones. The shear capacity of BFRP-reinforced recycled concrete beams without stirrups showed a linear relationship with the sectional effective depth d and was proportional to (d/a)4/3, (ρ)1/5 and ( )1/3. Meanwhile, four existing codes were used to predict the shear strength of test beams and the CSA806-02 gave the better results.

Climate control of the global tropical storm days (1965-2008) : CLIMATE CONTROL OF TROPICAL STORM DAYS

Wang, Bin,Yang, Yuxing,Ding, Qing-Hua,Murakami, Hiroyuki,Huang, Fei American Geophysical Union 2010 Geophysical research letters Vol.37 No.7

Genetic Diversity of a Chinese Native Chicken Breed, Bian Chicken, Based on Twenty-nine Microsatellite Markers

Ding, Fu-Xiang,Zhang, Gen-Xi,Wang, Jin-Yu,Li, Yuan,Zhang, Li-Jun,Wei, Yue,Wang, Hui-Hua,Zhang, Li,Hou, Qi-Rui Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

The level of genetic differentiation and genetic structure in a Chinese native chicken breed, Bian chicken, and two controlled chicken populations (Jinghai chicken and Youxi chicken in China) were analysed based on 29 microsatellite markers. A total of 166 distinct alleles were observed across the 3 breeds, and 32 of these alleles (19.3%) were unique to only 1 breed. Bian chicken carried the largest number of private alleles at 15 (46.9%), followed by the Jinghai chicken with 12 private alleles (37.5%). The average polymorphism information content (0.5168) and the average expected heterozygote frequency (0.5750) of the Bian chicken were the highest, and those of the Jinghai chicken were 0.4915 and 0.5505, respectively, which were the lowest. Among 29 microsatellite loci, there were 15 highly informative loci in Bian chicken, and the other 14 were reasonably informative loci. The highly informative loci in Jinghai chicken and Youxi chicken were 17 and 14 respectively. Significant deviations from the Hardy-Weinberg equilibrium were observed at several locus-breed combinations, showing a deficit of heterozygotes in many cases. As a whole, genetic differentiation among the breeds estimated by the fixation index (Fst) were at 6.7% (p<0.001). The heterozygote deficit within population (Fis) was 22.2% (p<0.001), with the highest (0.249) in Bian chicken and lowest (0.159) in Youxi chicken. These results serve as an initial step in the plan for genetic characterization and conservation of the Chinese chicken genetic resource of Bian, as well as Jinghai and Youxi chickens.

AlN with Strong Blue Emission Synthesized Through a Solventless Route

Wei Wang,Peng Zhang,Xiaobai Wang,Xiang Lei,Xiaodong Chen,Hong Ding,Hua Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.2

Aluminum nitride (AlN) with strong blue emission at 450 nm has been synthesized from hexahydrate aluminum chloride and urea above 850℃ via a solventless route. Several aluminum and nitrogen sources are selected and well investigated to determine the effect of AlN synthesis. Moreover, the structure, component, morphology, photoluminescence properties and possible synthesis mechanism are also discussed carefully.

Prognostic Value of Baseline and Interim Total Metabolic Tumor Volume and Total Lesion Glycolysis Measured on 18F-FDG PET-CT in Patients with Follicular Lymphoma

Jin-Hua Liang,Yun-Ping Zhang,Jun Xia,Chong-Yang Ding,Wei Wu,Li Wang,Lei Cao,Hua-Yuan Zhu,Lei Fan,Tian-Nv Li,Jian-Yong Li,Wei Xu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the prognostic significance of total metabolic tumor volume (TMTV) and total lesion glycolysis (TLG) in patients with follicular lymphoma (FL) at baseline and mid-treatment with 18F-fluorodeoxyglucose positron emission tomography computed tomography (PET-CT) scans. Materials and Methods The study analyzed data from 48 patients with FL who were treated in Jiangsu Province Hospital and reviewed their baseline PET-CT scans. TMTV and TLG were computed by using the absolute value of 2.0, 2.5, and 3.0 thresholding method, respectively. Results Median age was 53 years, 75.0% of patients had stage III to IV disease, 43.8% had a Follicular Lymphoma International Prognostic Index 1 (FLIPI1) score of 3 to 5 and 20.8% had a FLIPI2 score of 3 to 5. Receiver operating characteristic (ROC) curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 476.4 (sensitivity, 85.7%; specificity, 78.0%; area under the curve [AUC], 0.760; p=0.003) and 2,676.9 (sensitivity, 71.4%; specificity, 78.0%; AUC, 0.760; p=0.003). On multivariable analysis, TMTV3.0 and TLG3.0 were independent predictors of both progression-free survival (PFS) (hazard ratio [HR], 5.406; 95% confidence interval [CI], 1.326 to 22.040; p=0.019 and HR, 6.502; 95% CI, 1.079 to 39.182; p=0.042) and overall survival (OS) (HR, 4.111; 95% CI, 1.125 to 15.027; p=0.033 and HR, 5.885; 95% CI, 1.014 to 34.148; p=0.049). ROC curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 66.3% (sensitivity, 85.7%; specificity, 63.4%; AUC, 0.774; p < 0.001) and 64.5% (sensitivity, 85.7%; specificity, 65.9%; AUC, 0.777; p < 0.001). Conclusion Baseline TMTV and TLG are strong predictors of PFS and OS in FL. Furthermore, interim TMTV (TMTV > 66.3%) and TLG (TLG > 64.5%) reduction are valuable tools for early treatment response assessment in FL patients.

Ginsenoside compound K reduces the progression of Huntington's disease via the inhibition of oxidative stress and overactivation of the ATM/AMPK pathway

Kuo-Feng Hua,A-Ching Chao,Ting-Yu Lin,Wan-Tze Chen,Yu-Chieh Lee,Wan-Han Hsu,Sheau-Long Lee,Hsin-Min Wang,Ding-I. Yang,Tz-Chuen Ju 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4

Background: Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion oftrinucleotide CAG repeat in the Huntingtin (Htt) gene. The major pathogenic pathways underlying HDinvolve the impairment of cellular energy homeostasis and DNA damage in the brain. The protein kinaseataxia-telangiectasia mutated (ATM) is an important regulator of the DNA damage response. ATM isinvolved in the phosphorylation of AMP-activated protein kinase (AMPK), suggesting that AMPK plays acritical role in response to DNA damage. Herein, we demonstrated that expression of polyQ-expandedmutant Htt (mHtt) enhanced the phosphorylation of ATM. Ginsenoside is the main and most effectivecomponent of Panax ginseng. However, the protective effect of a ginsenoside (compound K, CK) in HDremains unclear and warrants further investigation. Methods: This study used the R6/2 transgenic mouse model of HD and performed behavioral tests,survival rate, histological analyses, and immunoblot assays. Results: The systematic administration of CK into R6/2 mice suppressed the activation of ATM/AMPK andreduced neuronal toxicity and mHTT aggregation. Most importantly, CK increased neuronal density andlifespan and improved motor dysfunction in R6/2 mice. Conversely, CK enhanced the expression of Bcl2protected striatal cells from the toxicity induced by the overactivation of mHtt and AMPK. Conclusions: Thus, the oral administration of CK reduced the disease progression and markedlyenhanced lifespan in the transgenic mouse model (R6/2) of HD.

Anchoring effect of Ni²⁺ in stabilizing reduced metallic particles for growing single-walled carbon nanotubes

He, Maoshuai,Wang, Xiao,Zhang, Lili,Wu, Qianru,Song, Xiaojie,Chernov, Alexander I.,Fedotov, Pavel V.,Obraztsova, Elena D.,Sainio, Jani,Jiang, Hua,Cui, Hongzhi,Ding, Feng,Kauppinen, Esko Elsevier 2018 Carbon Vol.128 No.-

<P><B>Abstract</B></P> <P>The suitability of the NiMgO catalyst as a catalyst in chiral-selective growth of single-walled carbon nanotubes (SWNTs) by chemical vapor deposition has been assessed. It reveals that catalyst calcination temperature plays an important role in affecting the catalyst performances. Using CO as the carbon precursor and a chemical vapor deposition reaction temperature of 600 °C, NiMgO pre-calcined at 600 °C demonstrates the best performances in catalyzing the growth of SWNTs with predominant (6, 5) species. Systematic characterizations on catalysts calcinated at different temperatures indicate that Ni<SUP>2+</SUP> ions diffuse towards the interior of MgO matrix upon annealing. DFT-based calculations reveal that the binding energy between Ni<SUP>2+</SUP> and adjacent Ni(0) is larger than that between Mg<SUP>2+</SUP> and Ni (0), while Ni<SUP>2+</SUP> situated deep inside MgO has weak interactions with surface Ni atoms. This work highlights the importance of subsurface Ni<SUP>2+</SUP> in anchoring reduced surface Ni atom, which inhibits the aggregation of Ni particles and therefore, facilitates the growth of SWNTs with a narrow chirality distribution.</P> <P><B>Graphical abstract</B></P> <P>A Ni-incorporated MgO catalyst was developed for predominant synthesis of (6, 5) single walled carbon nanotubes. Density functional theory-based calculations revealed that the unreduced subsurface Ni stabilized reduced Ni atoms on the surface, facilitating the growth of carbon nanotubes with a narrow chirality distribution.</P> <P>[DISPLAY OMISSION]</P>