EID3 Promotes Glioma Cell Proliferation and Survival by Inactivating AMPKα1

Xiang, Yaoxian,Zhu, Lei,He, Zijian,Xu, Lei,Mao, Yuhang,Jiang, Junjian,Xu, Jianguang The Korean Neurosurgical Society 2022 Journal of Korean neurosurgical society Vol.65 No.6

Objective : EID3 (EP300-interacting inhibitor of differentiation) was identified as a novel member of EID family and plays a pivotal role in colorectal cancer development. However, its role in glioma remained elusive. In current study, we identified EID3 as a novel oncogenic molecule in human glioma and is critical for glioma cell survival, proliferation and invasion. Methods : A total of five patients with glioma were recruited in present study and fresh glioma samples were removed from patients. Four weeks old male non-obese diabetic severe combined immune deficiency (NOD/SCID) mice were used as transplant recipient models. The subcutaneous tumor size was calculated and recorded every week with vernier caliper. EID3 and AMP-activated protein kinase α1 (AMPKα1) expression levels were confirmed by real-time polymerase chain reaction and Western blot assays. Colony formation assays were performed to evaluate cell proliferation. Methyl thiazolyl tetrazolium (MTT) assays were performed for cell viability assessment. Trypan blue staining approach was applied for cell death assessment. Cell Apoptosis DNA ELISA Detection Kit was used for apoptosis assessment. Results : EID3 was preferentially expressed in glioma tissues/cells, while undetectable in astrocytes, neuronal cells, or normal brain tissues. EID3 knocking down significantly hindered glioma cell proliferation and invasion, as well as induced reduction of cell viability, apoptosis and cell death. EID3 knocking down also greatly inhibited tumor growth in SCID mice. Knocking down of AMPKα1 could effectively rescue glioma cells from apoptosis and cell death caused by EID3 absence, indicating that AMPKα1 acted as a key downstream regulator of EID3 and mediated suppression effects caused by EID3 knocking down inhibition. These findings were confirmed in glioma cells generated patient-derived xenograft models. AMPKα1 protein levels were affected by MG132 treatment in glioma, which suggested EID3 might down regulate AMPKα1 through protein degradation. Conclusion : Collectively, our study demonstrated that EID3 promoted glioma cell proliferation and survival by inhibiting AMPKα1 expression. Targeting EID3 might represent a promising strategy for treating glioma.

Analysis of Germplasm in American Black Walnut by RAPD Markers

Xiang Shen,Zhi quan. Mao,Ji xiang. Zhang,Lei Wang,Jing Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

STUDY ON RESTRICTIVE FACTORS FOR TRAINING BASKETBALL RESERVE TALENTS IN CHINESE UNIVERSITIES

Xiang Lei,Kyoung-ho Choi,Eun-seok Yang 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2018 No.7

Basketball reserve talents’ quality largely decides the development of highlevel basketball team in Chinese universities. Due to lack of reserve talents, there is still big gap between actual effect of high-level basketball team in Chinese universities and original intention of team establishment. Main factors restricting the training of basketball reserve talents in Chinese universities include: (1) There is a small number of high-level traditional basketball project universities, and echelon construction faults; (2) In terms of teenage basketball players, internal motivation of exercise training is not enough, and basic quality is not high; (3) Scientific degree of reserve talents" training contents is not high, while the match is with small quantity and low level; (4) Integral level of teenage basketball coaches is not high; (5) Hardware facilities for training are not enough, and funding source is singe and insufficient; (6) Supporting management system is not perfect.

A Research on the Promotion of Basketball in Universities of China

Xiang-Lei(향뢰),Eun-Seok Yang(양은석) 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회논문지 Vol.12 No.6

본 연구는 중국 대학 농구 활성화 방안을 제시하기 위해 문헌과 설문 조사, 현장 방문과 관찰 방법을 통해 호남성 내 대학 농구 인재 양성 현황을 조사 및 분석 한 후, 인재 양성을 저해하는 요소를 파악하고, 활성화 방안에 대한 제시를 하였다. 본 연구 결과를 통해 대학 농구 인재 양성을 저해하는 7가지 주요 요소가 있음을 파악하였다. 첫째, 높은 수준의 농구팀을 가진 학교는 적었으며, 후진양성이 단절되어 있다. 둘째, 청소년 농구 선수들은 스포츠 훈련을 위한 내재적 동기가 부족하다. 셋째, 청소년 농구 선수의 기본 소양이 높지 않다. 넷째, 훈련의 과학화 수준이 높지 않고 경기 수가 적으며 수준이 낮다. 다섯째, 전반적으로 청소년 농구 코치의 수준이 높지 않다. 여섯째, 훈련 시설이 부족하며 자금 원천이 단일하고 불충분하다. 일곱째, 체계적인 관리 시스템이 부족하다. 따라서 호남성 내 대학 농구 인재 양성을 저해하는 요소들에 대한 활성화 방안에 대한 제안은 다음과 같다. 첫째, 농구 인재 양성 센터 설립을 집중적으로 추진한다. 둘째, 농구 인재 양성 센터는 협력을 기반으로 인재 양성 통합 시스템을 형성한다. 셋째, 전문 스포츠 학교의 장점을 활용하여 사회적 자원을 집중 투자하고 장려한다. 넷째, 코치의 직업 훈련 시스템을 개선하고 임금과 보너스를 적절하게 지급한다. 다섯째, 운동선수의 문화 수업을 엄격하게 관리하여 성적이 좋지 않은 경우 훈련과 경기에서 배제한다. 여섯째, 많은 경쟁 기회를 제공하고 다양한 형태의 농구 훈련반을 개설하며 캠프를 개최한다. Basketball reserve talents’ quality largely decides the development of high-level basketball team in Chinese universities. Due to lack of reserve talents, there is still big gap between actual effect of high-level basketball team in Chinese universities and original intention of team establishment. Main factors restricting the training of basketball reserve talents in Chinese universities include: first, There is a small number of high-level traditional basketball project universities, and echelon construction faults; second, In terms of teenage basketball players, internal motivation of exercise training is not enough, and basic quality is not high; third, Scientific degree of reserve talents" training contents is not high, while the match is with small quantity and low level; fourth, Integral level of teenage basketball coaches is not high; fifth, Hardware facilities for training are not enough, and funding source is singe and insufficient; sixth, Supporting management system is not perfect. It is suggested to get rid of the restrictive factors on training basketball reserve talents in Chinese universities through the following measures: first, training base of basketball reserve talents cooperates with university, forming integrated training and conveying; second, The professional advantage of sports school comes into play; it encourages social resources investment for joint development to increase the level of socialization; third, Improve coach position training system and properly increase coach"s bonus, wage etc.; fourth, Cultural courses of athletes shall be strictly required. If athletes do poorly in examination, stop training and match participation; fifth, Provide more competition opportunities and hold various forms of basketball training classes and summer camps.

Numerical Simulation for Synergetic Deformation of Optical Fiber Sensor and Asphalt Mixture

Xiang-fei Zhang,Zhen-dong Qian,Meng Zhang,Lei-lei Chen 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.7

Owing to the structural and material differences, data obtained by distributed optical fiber sensors (OFS) for asphalt pavement strain measurement lack of precision. For better understanding OFS measurement mechanism and investigating the measurement error correction, this study analyzed the synergetic deformation of OFS and asphalt mixture (AM) under load. Firstly, a threedimensional AM single-edge notched beam (SEB) model with OFS was established and verified using the discrete element method. Secondly, the micro-crack propagation of SEB model during loading procedure was analyzed to explore the debonding behavior between OFS and AM. Thirdly, strain development of OFS and AM was investigated during fracture stage and post-fracture stage. Finally, a measurement error correction method was developed based upon the materials and test conditions in this study. Results demonstrate that debonding occurs on OFS surface when macro-crack extends across OFS position. The key to making the strain measurement accuracy is to ensure the effective bonding between OFS and AM. In addition, this study provides a framework used to develop the measurement error correction, and the research results give the theoretical foundation for data selection and error correction of asphalt pavement strain measurement and crack detection using distributed optical fiber sensors.

Effects of comonomer with carboxylic group on stabilization of high molecular weight polyacrylonitrile nanofibrous copolymers

Lei, Danyun,Devarayan, Kesavan,Li, Xiang-Dan,Choi, Woong-Ki,Seo, Min-Kang,Kim, Byoung-Suhk 한국탄소학회 2014 Carbon Letters Vol.15 No.4

New precursors, poly(acrylonitrile-co-crotonic acid) (poly(AN-CA)) and poly(acrylonitrile-co-itaconic acid-co-crotonic acid) (poly(AN-IA-CA)) copolymers, for the preparation of carbon fibers, were explored in this study. The effects of comonomers with acidic groups, such as crotonic acid (CA) and/or itaconic acid (IA), on the stabilization of nanofibrous polyacrylonitrile (PAN) copolymers were studied. The extent of stabilization, evaluated by Fourier transform infrared spectroscopy, revealed that the CA comonomer could retard/control the stabilization rate of PAN, in contrast to the IA comonomer, which accelerated the stabilization process. Moreover, the synthesized PAN copolymers containing CA possessed higher Mv than those of the IA copolymers and also showed outstanding dimension stability of nanofibers during the stabilization, which may be a useful property for improving the dimensional stability of polymer composites during manufacturing.

The Effect of Impeller Indent Distance on the Performance of Vortex Pumps

Lei DAI,Lei GU,Jun WANG,Xiang AO,Xiaobo ZHU 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.1

Low efficiency is an urgent problem to be solved for vortex pumps due to large number of vortices and backflows. This paper concentrates on the performance improvement of vortex pumps caused by changing the indent distance of the impeller. First, a complete numerical model was established by selecting the optimal length of inlet and outlet and appropriate mesh quantity. Then, the reliability of this model was validated by comparing simulation results with experimental data. Based on this model, the internal flows of vortex pumps with different impeller indent distances were observed in simulations and the fitted curves of head and efficiency were created. Finally, the cause of the performance improvement was demonstrated by analyzing the flow field in vortex pumps.

Deep auto-context convolutional neural networks for standard-dose PET image estimation from low-dose PET/MRI

Xiang, Lei,Qiao, Yu,Nie, Dong,An, Le,Lin, Weili,Wang, Qian,Shen, Dinggang Elsevier 2017 Neurocomputing Vol.267 No.-

<P><B>Abstract</B></P> <P>Positron emission tomography (PET) is an essential technique in many clinical applications such as tumor detection and brain disorder diagnosis. In order to obtain high-quality PET images, a standard-dose radioactive tracer is needed, which inevitably causes the risk of radiation exposure damage. For reducing the patient's exposure to radiation and maintaining the high quality of PET images, in this paper, we propose a deep learning architecture to estimate the high-quality standard-dose PET (SPET) image from the combination of the low-quality low-dose PET (LPET) image and the accompanying T1-weighted acquisition from magnetic resonance imaging (MRI). Specifically, we adapt the convolutional neural network (CNN) to account for the two channel inputs of LPET and T1, and directly learn the end-to-end mapping between the inputs and the SPET output. Then, we integrate multiple CNN modules following the auto-context strategy, such that the tentatively estimated SPET of an early CNN can be iteratively refined by subsequent CNNs. Validations on real human brain PET/MRI data show that our proposed method can provide competitive estimation quality of the PET images, compared to the state-of-the-art methods. Meanwhile, our method is highly efficient to test on a new subject, e.g., spending ∼2 s for estimating an entire SPET image in contrast to ∼16 min by the state-of-the-art method. The results above demonstrate the potential of our method in real clinical applications.</P>

Personalized crawling: a data mining approach to topic-specific web resource discovery

Lei xiang,Yajun Du,Zhanbing Dong 대한전자공학회 2006 ITC-CSCC :International Technical Conference on Ci Vol.2006 No.3

Meta-analysis of Gene Expression Data Identifies Causal Genes for Prostate Cancer

Wang, Xiang-Yang,Hao, Jian-Wei,Zhou, Rui-Jin,Zhang, Xiang-Sheng,Yan, Tian-Zhong,Ding, De-Gang,Shan, Lei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Prostate cancer is a leading cause of death in male populations across the globe. With the advent of gene expression arrays, many microarray studies have been conducted in prostate cancer, but the results have varied across different studies. To better understand the genetic and biologic mechanisms of prostate cancer, we conducted a meta-analysis of two studies on prostate cancer. Eight key genes were identified to be differentially expressed with progression. After gene co-expression analysis based on data from the GEO database, we obtained a co-expressed gene list which included 725 genes. Gene Ontology analysis revealed that these genes are involved in actin filament-based processes, locomotion and cell morphogenesis. Further analysis of the gene list should provide important clues for developing new prognostic markers and therapeutic targets.