결핵성 육아종에서 Thioredoxin peroxidase-2 의 발현

박근호,유형륜,정영진,윤기중,한원철,유대열,문형배 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: Thioredoxin peroxidase(TPX) is a kind of recently discovered antioxidant enzyme which react as rapid hydrogen ion donor for the removal of hydroperoxide. The action and distribution of the TPX was poorly understood in the human diseases. This experiments were designed for the study about the distribution of the TPX in the chronic granulomatous inflammation and about the correlation between the expression of TPX and the site of inflammation, histological activities of tuberculous inflammation or existence of mycobacterium in the inflammatory foci. Methods: The immunohistochemical stains were performed for the localization of the TPX-2 in the epithelioid cells, giant cells and lymphocytes in the chronic granulomatous inflammation. The tissue sections were obtained from the paraffin blocks of the 54 cases of tuberculosis (lung 21 cases, lymph node 12 cases, bone and soft tissue 12 cases, kidney 9 cases; active 33 cases, inactive 21 cases by the histologic classification; presence of mycobacterium 15 cases, no mycobacterium 39 cases by PCR reaction). Results: The expression of TPX-2 was 16.7% in the giant cells, 27.8% in the epithelioid cells and 100% in the lymphocytes of tuberculous inflammations. The expression of TPX-2 in the giant cells and epithelioid cells of the tuberculosis were 28.6% and 57.1% of the pulmonary tuberculosis; 33.3% in each cells of the renal tuberculosis; 0% in each cells of the lymph node or bone and soft tissue tuberculosis. The expression of TPX-2 in the giant cells and epithelioid cells were 9.1% in each cells of the active tuberculosis and were 28.6% and 57.1% in each cells of the inactive tuberculosis by histologic classification. The expression of TPX-2 in the giant cells and epithelioid cells was 40% in each cells of tuberculosis which mycobacteria were detected and the expression of TPX-2 was 7.7% and 23.1% in each cells which mycobacteria were not detected by PCR reaction in the paraffin embedded tissue. Conclusions: The above results were summarized that the TPX-2 in the giant cells and epithelioid cells were more frequently expressed in the inactive tuberculosis than in the active tuberculosis. These results suggest that the TPX-2 is a kind of regulating or suppressing factors in the activity of the tuberculosis.

大學卒業定員制의 實施에 따른 學生生活 指導方案에 關한 硏究

崔根爀,張忠根,李相祐,張鎭吉,張大熙 충남대학교 1982 學生生活硏究 Vol.10 No.1

Higher education in Korea is faced with a new situation since 1981 when the government implemented a college graduation quota system designed to broaden the opportunities for higher education, alleviate the problem of the students who fail the entrance examination, and improve the quality of college graduates. The change of the system has brought about several serious problems including how to supply enough faculty members, how to provide enough educational facilities, and how to cope with the huge increase in the number of students, 30% more than graduation quota. The greatest single problem resulting from the implementation of college graduation quota system is the psychological and emotional unease aroused among the students, because 30% of those students with unsatisfying results must be banished from college before their graduation. This study aims to find effective measures for student guidance that each of the colleges can implement to help students adjust to the changed circumstances. After objectively investigating the problems from the various educational environments, from the recent students' views of college, and from the psychological and emotional unease arising in them by the fear of failing to graduate from their college because of implementation of the graduation quota system, a survey was conducted by means of a questionnaire composed of 40 questions and by means of personal interviews, 5,903 students, who are influenced by the graduation quota system, attending 19 public and private universities and colleges selected throughout the country. The data obtained from this survey were statistically processed with the help of the C. N. U. Computer Center, and the ratios of the responses to each of the questions were analyzed and calculated according to the distinctions of the total, sex, region, and major. The conclusions that the interrelations among the variant factors from each of the questions which were surveyed, analyzed and arranged have suggested are as follows : 1. The original purpose of graduation quota system should continually be clarified and communicated to all students and a concrete, specialized guidance system should be established to guide the minority of the students (13%) who don't fully understand the basic properties of higher educational institutions. 2. The institutions should provide enough educational facilities and supply enough faculty memebers to solve the problem of accomodating the large numbers of students. At the same time, they should suggest how students should learn positively and effectively to gain an expert knowledge, and how professors should gain public confidence in grade evaluation. Accompanying those measures, the government should develop a good policy to expand and distribute job opportunities to college graduates. 3. The admission quota should be controlled depending on the rate of naturally failed students, and then the students' unease and suppression caused by excessive competition can be relieved. In addition, it is advisable that the banished students should be allowed to transfer to University of the Air without regard to their grades. 4. An education devised to let students have a sound sense and way of thinking should be accompanied by a guidance system which their seniors, parents and society take part in. 5. An atmosphere conducive to sweet and well-rounded friendship among classmates should be created, sound extra-curricular activities should be encouraged and the number of students and adviser professor is in charge of should be lessened to a reasonable number.

레진강화형 글라스아이오노머의 2 축 굽힘강도

이용근,임미경,구대회,이정식 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

Resin-modified glass ionomers were introduced in 1988 to overcome the problems of moisture sensitivity and low early mechanical strengths of the conventional glass ionomers, and to maintain their clinical advantages. The purpose of this study was to evaluate the bi-axial fracture strength of four resin-modified glass-ionomers(Fuji II LC, Vitremer, Dyract, VstiGlass), one resin composite material(Z-100), and one conventional glass-ionomer(Fuji II). Three specimens of each material and shade combination were made according to the manufacturers' instructions. Materials were condensed into metal mold with a diameter of 10mm and a thickness of 2.0mm and pressed between two glass plates. Resin-modified glass ionomers were polymerized using a Visilux II light curing unit by irradiating for 60 seconds from both sides, and conventional glass ionomer was cured chemically. After specimens were removed from the molds, surfaces were polished sequentially on wet sandpapers up to No. 699 silicone carbide paper. The specimens were thermocycled for 2,000 cycles between 5℃ and 55℃ distilled water. After thermocycling, bi-axial fracture strengths were measured using a compressive-tensile tester(Zwick 1456 Z020, Germany) with the cross head speed of 0.5mm/minute. The results were as follows: 1. Two factors of the kind and color of materials had a main effect on bi-axial fracture strength (p<0.01), and bi-axial fracture strength was influenced significantly by the kinds of materials (p<0.01). But there was no significant interaction between two variables of the kind and color of materials (p>0.05). 2. Comparing the mechanical properties of the materials, the elastic modulus of Z100 was higher than any other material, and there was no difference in the displacement at fracture among materials. The bi-axial fracture strength of Z100 was significantly higher than any other material, and that of resin-modified glass ionomers was significantly higher than that of conventional glass ionomer (p<0.05). 3. In the same material group, the color of material had little influence on the mechanical properties.

CMOS 단일칩 마이크로컴퓨터의 제어논리 설계

한대근,정호선,이우일 경북대학교 공과대학 1986 工大硏究誌 Vol.15 No.-

The control part of CMOS single chip micro-computer has been designed and simulated with GLOG. The circuits have been implemented using SPICE simulator. Results of logic simulation and circuit simulation were in good agreements with expected circuit characteristics.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

도심터널 굴착으로 인한 주변지반의 변위에 관한 연구

이유근,정대석 진주산업대학교 산업과학기술연구소 1996 산업과학기술연구소보 Vol.- No.3

이상과 같이 쌍터널(twin tunnel)에 대한 수치 해석 결과 주변지반의 안정성 확보를 위해 다음과 같은 결론을 얻게 되었다. 1) 횡방향 지표침하는 가우스정규분포곡선으로, 종방향 지표침하는 중첩(Superposition)시킨 누적정규분포곡선들로서 해석이 가능하다. 2) 1차 및 2차 터널의 굴진간격차가 좁은 국내NATM시공의 경우, 종방향 지표침하를 해석할 수 있는 침하곡선식을 제안하였다. y/Z_0 ≥0 경우, U+(1-A)exp(B'y/Z_0) y/Z_0≥0 경우, U=1-A exp(-B(y/Z_0)) 단, A = 0.65, B'= B (일반 국내 NATM 시공 경우) 3) d<0.8 Z_0 경우에는 지질조건에 관계없이 횡방향 침하곡선형태는 Unimodal로, d≒Z_0 경우에는 점성토 및 사질토 모든 지반에서 Bimodal로 나타난다. 4) 지중침하는 양터널 중간부가 작게 나타나고 터널 중심부가 최대로 나타나는 W형 (Bimodal)의 침하곡선형태를 갖는다. 5) 매개변수중, 터널직경에 대한 pillar폭의 비, S'/D이 지반침하간섭의 가장 큰 영향인자이며, S'/D 의 감소시 간섭체적이 증가하나 S'/D≤0.5인 경우는 압축상태의 체적 변형을 갖는 pillar의 폭 감소로 오히려 간섭체적이 감소한다. 6) 평행하지 않는 쌍터널의 경우, 1,2차 터널의 깊이비 Z_02/Z_01≥1 일때 침하간섭체적이 증가하고 Z_02/Z_01<1 일때 감소하므로 실제 현장에서는 터널깊이가 작은 터널을 1차시공하는 것이 유리하며 1,2차터널의 단면적 비 A_2/A_1>1 일때 간섭체적이 감소하고 A_2/A_l<1 일때 증가하므로 단면적이 적은 터널을 1차 굴진하는 것이 유리하다. A tuunel in an urban area should be to predict the magnitude and distribution of ground movements which are important to investigate the potential damage to the existing structure around tunnel. The present study examines available theories and emprical equations, and tries to investigate quantativily ground movements around tunnel with tunnel progressing. Approaches to the problem of ground movements associated with twin tunnel was and elasto- platic finite element method.

Rat의 복강에 삽입한 Fluorouracil-Polyglycolic acid 제형의 Fluorouracil 용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitoneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adcnocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

Rat의 복강에 삽입한 Filorouracil-Polyglycolic acid제형의 Fluorouracil용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitioneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adenocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.