흰쥐에서 갑상샘 절제 후 재생에 따른 세포 및 세포사이물질의 변화

황영일,정용환,이왕재,황덕호,유영복,김혜숙,장가용 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.4

경희대학교 국제캠퍼스 걷고 싶은 거리 조성계획

김동찬,김영준,마원,김충희,노경식,이가영 경희대학교 부설 디자인연구원 2008 예술· 디자인학연구 Vol.11 No.1

Kyung-Hee University Global Campus accepted changing of education environment to adapt to the World's information technology society so that school has been preparing the Master plan to grow up campus's vision since 2007. However, realization of the Master plan need a lot of financial resources, much times and phases in improvement. Especially, our university needs symbolized business in 2009 due to the 60th anniversary of our university. Based on this we want to improve the main entrance road environment from school gate to the school of international management and reconstruct the spatial environment beside the main road. The purpose of this research is to construct a pedestrian space environment and reconstruct the campus environment which adapt to the new times' demands. That is why the Master plan is trying to find the landscape scenic which adapts to our school' s main entrance space and the improvement method about the pedestrian space of the Global campus to congratulate of our university's 60th anniversary.

경희대학교 서울캠퍼스 진입로 및 주변 보행공간 기본계획

김동찬,김영준,마원,김충희,노경식,이가영 경희대학교 부설 디자인연구원 2008 예술· 디자인학연구 Vol.11 No.1

Kyung-Hee University in Seoul accept changing of education environment to deal with World's information technology society so that school had preparing the Masterplan to grow up campus's vision since 2006th. Finally we finish up this plan. However, realization of the Masterplan need a lot of financial resources, much times and phases in improvement. Especially, our school needs symbolized business in 2009 due to school of 60th anniversary. So we made a plan that to construct by design which is going to reshape campus's main road and westside afforest place for future. As a result, purpose of research is to improve an atmosphere conducive to academic pursuit, to show up greatness of school and to deal with changing school's environment rapidlly. That is why the Masterplan is trying to find school of superb scenic and developing of environment to congratulate of our school's 60th anniversary main approach and walking spaces.

캡슐내시경과 단일풍선 소장내시경으로 진단된 공장의 간질종양 1예

송도경,심기남,태정현,김경진,송명은,송하응,윤혜원,정가영,정정화 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.2

Gastrointestinal stromal tumors (GISTs) are common mesenchymal tumors that arise in the wall of the gastrointestinal tract. We report a case of obscure gastrointestinal bleeding due to a GIST of the jejunum successfully documented by videocapsule endoscopy (VCE) and single-balloon enteroscopy (SBE). A 36-year-old man with hematochezia was referred for further evaluation of no evidence of bleeding focus on esophagogastroduodenoscopy and colonoscopy. A VCE showed a suspicious ulcerative hyperemic mass that located in about 1 hour apart from duodenal second portion. SBE revealed a nonbleeding 4×2 cm mass with an ulcer at the proximal jejunum. The patient underwent laparoscopic resection without complication. Histological examination revealed a well circumscribed, dumbbell-shaped firm mass comprised of spindle cells. Immunohistochemical staining for CD 117 was diffusely positive, whereas staining for S-100, CD 34 and MIB-1 was all negative. It was confirmed to be a low-grade GIST at the proximal jejunum.

식도암과 위암이 동시에 발생한 원발성 중복암 1예

윤혜원,심기남,나선경,송도경,정정화,정가영 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.2

Double primary cancers are two independently developed cancers in an individual. There have been some reports on double primary cancer since Billroth reported it for the first time in 1879. Double primary cancer of the stomach and esophagus has been revealed a very low incidence worldwide. The incidence of an esophageal cancer with another primary cancer is reported to be 9.5∼27%, but double primary cancers in the esophagus and stomach have been rarely reported to our knowledge. In this study, we present here a case of double primary esophageal and stomach cancer in a 66-year-old man because of progressive dysphagia.

말초혈액단핵구와 화학적으로 손상된 각막세포의 혼합반응에 대한 시스테아민의 효과

이영복(Young Bok Lee, MD),현준영(Joon Young Hyon, MD, PhD),위원량(Won Ryang Wee, MD, PhD),정태영(Tae Young Chung, MD, PhD),정의상(Eui Sang Chung, MD, PhD),이가영(Ka Young Yi, MD, PhD),신영주(Young Joo Shin, MD, PhD) 대한안과학회 2015 대한안과학회지 Vol.56 No.10

목적: 말초혈액단핵구와 화학적으로 손상된 각막세포의 혼합반응에 대한 시스테아민의 효과를 알아보고자 하였다. 대상과 방법: 사람각막간질세포를 0.05 N NaOH로 60초간 처리하여 화학적으로 손상시킨 후 말초혈액단핵구자극 검사를 시행하였다. 혼합 말초혈액단핵구-각막세포반응은 이후 다양한 농도의 시스테아민(0-10 mM)으로 처리되었다. 세포 내 활성산소물질 형성 정도는 산화에 민감한 형광탐침자인 2′,7′-dichlorofluorescein diacetate (DCF-DA)를 사용하여 측정되었다. NaOH로 처리된 각막세포에 의해 촉진된 말초혈액단핵구의 증식 속도와 염증성 사이토카인 분비는 각각 bromodeoxyuridine 증식분석검사와 효소결합 면역흡수분석법으로 측정하였다. 결과: 말초혈액단핵구 증식은 시스테아민의 농도에 비례하여 억제되었다(p=0.019). DCF-DA 형광은 시스테아민 농도에 비례하여 감소하였다(p<0.001). 시스테아민의 농도에 비례하여 기질분해효소-9 (matrix metalloprotease-9, MMP-9), 인터류킨-6 (interleukin-6, IL-6), 형질전환성장인자-베타1 (transforming growth factor-beta 1, TGF-β1)은 억제되었고(p<0.05), 대식세포이동저해인자(macrophage migration inhibitory factor, MIF)는 증가하였다(p=0.008). 결론: 시스테아민이 활성산소물질의 억제를 통하여 화학적으로 손상된 각막세포에 의해 유발된 말초혈액단핵구의 증식과 염증 사이토카인의 분비를 억제하였다. 따라서 시스테아민이 화학적 각막손상 환자에서 치료제로 사용될 수 있을 것으로 기대된다. Purpose: To investigate the effect of cysteamine on mixed peripheral blood mononuclear cells (PBMCs)-chemically injured keratocytes reaction (mixed lymphocyte-keratocyte reaction; MLKR). Methods: PBMC stimulation assay was performed after keratocytes were chemically injured with 0.05 N NaOH for 60 seconds. MLKR was treated with various concentrations of cysteamine (0-10 mM). Intracellular reactive oxygen species (ROS) formation was measured using the oxidation-sensitive fluorescent probe, 2′7′-dichlorofluorescein diacetate (DCF-DA). Proliferation rate of PBMCs stimulated by NaOH-treated keratocytes and secretion profiles of matrix metalloprotease-9 (MMP-9), transforming growth factor-beta1 (TGF-β1), interleukin-6 (IL-6), and macrophage migration inhibitory factor (MIF) were determined using the bromodeoxyuridine proliferation assay and enzyme-linked immunosorbent assay, respectively. Results: Proliferation rate of PMBCs was suppressed by cysteamine in a dose-dependent manner (p = 0.019). Fluorescence of DCF-DA decreased depending on cysteamine concentration (p < 0.001). MMP-9, IL-6 and TGF-β1 levels were suppressed by cysteamine in a dose-dependent manner (p < 0.05), whereas MIF levels increased with cysteamine concentration of 0.5-10 mM (p = 0.008). Conclusions: These study results indicate that cysteamine induced the ROS-mediated inhibition of inflammatory cytokine release and proliferation of PBMCs stimulated by chemically injured keratocytes. Thus, cysteamine can be used in the treatment of chemical corneal burns.

철학적 탐구공동체 활동이 유아의 인성에 미치는 영향

박가영 ( Ka-young Park ),정대련 ( Dae Ryun Chung ) 한국어린이문학교육학회 2016 어린이문학교육연구 Vol.17 No.2

본 연구는 유아교육기관에서의 철학적 탐구공동체 활동이 유아 인성에 어떠한 영향을 미치는지 알아보려는 목적으로 시도되었다. 연구대상은 경기도 G시에 위치한 시립 T어린이집 2개 학급의 5세 유아 38명이다. 실험집단 유아에게는 연구자가 주 2회씩 22회차 동안 선정된 10권의 그림책을 활용하여 철학적 탐구공동체 활동을 실시하였으며, 비교집단 유아에게는 담임교사가 동일한 그림책을 활용하여 이야기나누기 활동을 실시하였다. 연구 결과, 첫째 철학적 탐구공동체 활동에 참여한 실험집단과 참여하지 않은 비교집단 간 유아의 인성차원의 총점에서 유의한 차이가 나타났다. 둘째, 철학적 탐구공동체 활동이 실험집단 유아의 인성차원별 즉 개인, 대인 및 사회 차원의 변화에 긍정적인 효과를 미치고있는 것으로 나타났다. 셋째, 토의 중 교사-유아, 유아-유아의 언어적 상호작용 양상에 있어서는 인성의 세 가지 차원과 9개 하위요소가 복합적으로 나타났다. 결론적으로, 유아들이 철학적 탐구공동체 활동에 참여하여 인성 덕목과 가치에 관련된 사고하기와 판단하기, 그리고 사고 성향이나 태도를 내면화 할 수 있는 경험을 함으로써, 유아기부터 자신과 타인, 더 나아가 사회적 존재로서 적합한 인성을 함양할 수 있다는 가능성을 보였다는 데서 본연구의 의의를 찾을 수 있다. The purpose of this study was to apply and analyze the activities that build a community of philosophical inquiry using 10 picture books related to young children’s character development. As research subjects, this study selected 38 5-year-old children from a nursery school in G city, Gyeonggi-do, and conducted the activities that build a community of philosophical inquiry with 19 children in the experimental group for 22 sessions (30-40 minutes per session) and estimated the changes with the 「Humanity Rating Scale for 3-5 year-old Children」. The results are as follows. First, there were positive changes in the total points for the experimental group. Second, some meaningful differences were observed between the experimental and comparative group in the three dimensions of character value,:personal, interpersonal, and social value. Third, the experimental group showed positive changes in 9 factors for the 3 dimensions,:self -establishment, positive attitude toward life, aesthetic attainment, sense of moral responsibility, moral judgment, respect for other people, forgiveness in others and generosity, duty and role as a member of society, and cosmopolitan spirit during the activities and daily lives in the classroom. In conclusion, 5 procedures for building a community of philosophical inquiry could help young children improve their character development by using the 10 picture books examined in this study.

Perception of Child Abuse and Child Disciplinary Practice among Adults Abused as Children

Ka Young Moon,So Young Irene Lee,A Reum Lee,Ka Yeong An,Kyung Soo Jung,Kyoung-Il Paek,Hyun Ah Kang,Ji Young Kang,Shun Ah Chung 대한소아청소년정신의학회 2019 소아청소년정신의학 Vol.30 No.2

Objectives: The aim of this study was to compare differences in perception and knowledge of child abuse and child disciplinary practices according to the history of child abuse victimization. Methods: A questionnaire survey on child abuse was conducted with 491 adults raising children. We compared the perception and knowledge of child abuse and child disciplinary practices between two groups of adults with and without a history of childhood abuse victimization. Results: The group with a history of childhood abuse had lower levels of knowledge of child abuse (F=6.990, p<0.01) and engaged in more negative disciplinary practices (F=5.974, p<0.05) than those without. However, no differences in the perception of child abuse were observed between the two groups. Conclusion: The results suggest that adults with a history of childhood abuse have lower levels of knowledge of child abuse and use more negative disciplinary practices in raising their children. This highlights the need to administer not only educational but also more direct hands-on interventions to vulnerable parents in order to foster healthy parenting and disciplinary practices.

Structural Aspects of GPCR-G Protein Coupling

Ka Young Chung 한국독성학회 2013 Toxicological Research Vol.29 No.3

G protein-coupled receptors (GPCRs) are membrane receptors; approximately 40% of drugs on the market target GPCRs. A precise understanding of the activation mechanism of GPCRs would facilitate the development of more effective and less toxic drugs. Heterotrimeric G proteins are important molecular switches in GPCR-mediated signal transduction. An agonist-activated receptor interacts with specific sites on G proteins and promotes the release of GDP from the Gα subunit. Because of the important biological role of the GPCR-G protein coupling, conformational changes in the G protein upon receptor coupling have been of great interest. One of the most important questions was the interface between the GPCR and G proteins and the structural mechanism of GPCR-induced G protein activation. A number of biochemical and biophysical studies have been performed since the late 80s to address these questions; there was a significant breakthrough in 2011 when the crystal structure of a GPCR-G protein complex was solved. This review discusses the structural aspects of GPCR-G protein coupling by comparing the results of previous biochemical and biophysical studies to the GPCR-G protein crystal structure.

Structural Aspects of GPCR-G Protein Coupling

Chung, Ka Young Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.3

G protein-coupled receptors (GPCRs) are membrane receptors; approximately 40% of drugs on the market target GPCRs. A precise understanding of the activation mechanism of GPCRs would facilitate the development of more effective and less toxic drugs. Heterotrimeric G proteins are important molecular switches in GPCR-mediated signal transduction. An agonist-activated receptor interacts with specific sites on G proteins and promotes the release of GDP from the $G{\alpha}$ subunit. Because of the important biological role of the GPCR-G protein coupling, conformational changes in the G protein upon receptor coupling have been of great interest. One of the most important questions was the interface between the GPCR and G proteins and the structural mechanism of GPCR-induced G protein activation. A number of biochemical and biophysical studies have been performed since the late 80s to address these questions; there was a significant breakthrough in 2011 when the crystal structure of a GPCR-G protein complex was solved. This review discusses the structural aspects of GPCR-G protein coupling by comparing the results of previous biochemical and biophysical studies to the GPCR-G protein crystal structure.