N4SSB 단백질의 C-말단기의 7개의 아미노산이 N4SSB 단백질의 in vivo 활성에 미치는 영향

최미영,Choi, Mieyoung 한국미생물학회 1998 미생물학회지 Vol.34 No.4

Bacteriophage N4, a lytic phage specific for Esherichia coli K12 strain encodes single-stranded DNA-binding protein, N4SSB (bacteriophage N4-coded single-stranded DNA-binding protein). N4SSB protein is originally identified as a protein required for N4 DNA replication. N4SSB protein is also required for N4 late transcription, which is catalyzed by E. coli ${\sigma}^{70}$ RNA polymerase. N4 late transcription does not occur until N4SSB protein is synthesized. Recently it is reported that N4SSB protein is essential for N4 DNA recombination. Therefore N 4SSB protein is a multifunctional protein required for N4 DNA replication, late transcription, and N4 DNA recombination. In this study, a variety of mutant N4SSB proteins containing internal deletions or substitutions were constructed to define and characterize domains important for N4 DNA replication, late transcription, and N4 DNA recombination. Test for the ill vivo activity of these mutant N4SSBs for N4 DNA replication, late transcription, and N4 DNA recombination was examined. The results suggest that C-terminal 7 amino acid residues are important for the activity of N4SSB. Three lysine residues, which are contained in this region play important roles on N4SSB activity. Esherichia coli(E. coli) K12 균주를 숙주세포로 삼는 박테리오파아지인 N4는 single-stranded DNA에 결합하는 단백질인 N4SSB(bacteriophage N4-coded single-stranded DNA-binding protein) 단백질을 만든다. N4SSB 단백질은 N4 DNA replication 뿐만 아니라 late transcription과 N4 DNA recombination에도 필요한 여러 가지 기능을 가진 단백질이다. N4 late transcription은 숙주세포인 E. coli의 $E{\sigma}^{70}$ RNA polymerase에 의해서 수행이 되나 N4SSB 단백질을 반드시 필요로 하기 때문에 N4SSB 단백질이 생성될 때까지는 N4 late promoter로부터 RNA 합성이 일어나지 않는다. 본 연구에서는 N4SSB의 N4 DNA replication과 late transcription, 그리고 N4 DNA recombination에 필요한 영역(domain)을 알아내기 위해서 여러 가지 돌연변이형 N4SSB 단백질을 만들어 N4 DNA replication과 late transcription, 그리고 N4 DNA recombination의 3가지 작용에 대한 in vivo 활성을 조사 분석하였다. 그 결과 N4SSB 단백질의 C-말단기에 있는 7개의 아미노산이 N4SSB 단백질의 활성에 중요하다는 것을 알 수 있었다. 특히 C-말단기의 7개의 아미노산에는 세 개의 lysine이 포함되어 있는데 이 lysine이 N4SSB 단백질의 활성에 중요한 역할을 한다는 것이 제시되었다.

대구시 하수처리장 운전실태에 따른 중수도 도입 방안 연구

최미영,나욱호,홍원화,김삼열,Choi Mi-Young,Na Wook-Ho,Hong Won-Hwa,Kim Sam-Yeul 한국주거학회 2005 한국주거학회 논문집 Vol.16 No.1

It has seen the significant increase of water consumption rate due to rapid industrialization, high-densities of city, and increasing the population; thereby leads further water resource required in near future. In order to solve this deficit there are one of solution that gray water method demonstrates advantages regarding the questions of optimal water utility and thereby master plan of water. The method of study is analysis of investigation data through interview and research of sewage treatment plants in Daegu City. Through these analysis, this paper examines the capacity of graywater and optimal graywater facility.

소아 눈모음부족외사시에서 근거리/원거리 사시각에 따른 단안 절제술 및 후전술

최미영,황정민.Mi-Young Choi. M.D.. Jeong-Min Hwang. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.10

Purpose: The purpose of this study was to evaluate prospectively the long-term surgical results of unilateral lateral rectus (LR) muscle recession and medial rectus (MR) muscle resection in children with convergence insufficiency exotropia (XT). Methods: This prospective study included 14 children with an angle of XT greater at near than at distance by 10 prism diopters (PD) or more. The amounts of resection and recession were based on near and distance deviation, respectively. Minimum follow-up was one year (mean, 26.6±19.4 months) after surgery. Results: Significant postoperative reduction was achieved in mean distance exodeviation from 22.5 PD to 9.1 PD (p=0.000), and in mean near exodeviation from 33.8 PD to 13.6 PD (p=0.000). The mean near-distance difference decreased from 11.3 PD preoperatively to 4.6 PD postoperatively (p=0.000). A Fresnel prism was used temporarily to treat postoperative esotropia in only one patient for 6 months postoperatively. Conclusions: Unilateral surgery biased more to MR strengthening than to LR weakening in children with convergence insufficiency XT, was found to successfully reduce both distance and near deviation and to collapse near-distance differences with a low risk of long-term postoperative esotropia.

5-11세 소아에서 코로나19 백신의 효능 및 안전성에 대한 체계적 문헌고찰

최미영,유수연,정채림,최영준,최수한,Choi, Miyoung,Yu, Su-Yeon,Cheong, Chelim,Choe, Young June,Choi, Soo-Han 대한소아감염학회 2022 Pediatric Infection and Vaccine Vol.29 No.1

Purpose: To evaluate the efficacy and safety of coronavirus disease 2019 (COVID-19) vaccines in children aged 5-11 years, a rapid systematic review was conducted on published clinical trials of COVID-19 vaccines and studies that analyzed real-world data on adverse events after COVID-19 vaccination. Methods: A systematic search was conducted on medical literature in international (Ovid-MEDLINE) and pre-published literature databases (medRxiv), followed by handsearching up to January 4, 2022. We used terms including COVID-19, severe acute respiratory syndrome coronavirus 2, and vaccines, and the certainty of evidence was graded using the GRADE approach. Results: A total of 1,675 studies were identified, of which five were finally selected. Among the five studies, four consisted of data from clinical trials of each of the four types of COVID-19 vaccines (BNT162b2, mRNA-1273, CoronaVac, and BBIBP-CorV). The remaining study consisted of real-world data on the safety of the BNT162b2 vaccine in children aged 5-11 years. This systematic review identified that COVID-19 vaccines in recipients aged 5-11 years produced a favorable immune response, and were vaccines were effective against COVID-19. The safety findings for the BNT162b2 vaccine in children and early adolescents aged 5-11 years were similar to those data noted in the clinical trial. Conclusions: There is limited data on COVID-19 vaccines in children aged 5-11 years. Consequently continuous and comprehensive monitoring is necessary for the evaluation of the safety and effectiveness of the COVID-19 vaccines.

기질약시에 의한 소아 감각사시의 임상 분석

최미영,황정민,Mi-Young Choi,Jeong-Min Hwang 대한안과학회 2005 대한안과학회지 Vol.46 No.8

Purpose: To study the clinical characteristics of pediatric sensory strabismus in Korean children. Methods: A retrospective analysis was performed on 71 patients with the diagnosis of sensory strabismus before the age of 16 years. Patients with strabismic amblyopia or anisometropic amblyopia were excluded from consideration. The age at onset of vision loss, diagnosis, type of strabismus, deviated angle, etiologic factors leading to vision loss, and visual acuity of the deviated eye were recorded. The surgical results were analyzed in the case of strabismus surgery for sensory strabismus. Results: The mean age at the onset of vision loss was 4.6 years and the mean age at diagnosis was 7.5 years. Forty-one patients (58%) had congenital vision loss and the most common cause of vision loss was optic nerve disease in 35 (49%). Exotropia developed in 58 (82%), and patients with severely impaired visual acuity were more likely to develop exotropia (P=0.054). The age at the onset of vision loss and the age at diagnosis in esotropia were younger than in exotropia (P=0.049, P=0.047, respectively). Twenty-five (78%) of 32 patients who had undergonewere strabismus surgery had ocular alignment within 10 prism diopters of orthophoria. The frequency of consecutive exotropia was 40% in surgery for esotropia. Conclusions: Children with organic amblyopia tend to develop sensory exotropia. We considered that the age at initial vision loss and the severity of vision loss could play a role in determining the direction of deviation. The surgical results were favorable, but we should pay attention to the development of consecutive exotropia in surgery for esotropia.

한 눈의 시력이 0.1 이하인 순수 굴절부등약시의 임상 분석

최미영,김연희,Mi Young Choi,Youn Hui Kim 대한안과학회 2008 대한안과학회지 Vol.49 No.6

Purpose: To analyze the results of treatment in pure anisometrpic amblyopia with unilateral visual acuity of 0.1 or worse. Methods: The major eligibility criteria included pure anisometropic amblyopia with age <10 years, best-corrected visual acuity 0.1 or worse in the worse eye and 0.5 or better in the good eye after the wearing of optimal spectacle correction for a minimum of 4 weeks, and the minimum follow-up was 6 months. Twenty-one patients were selected and we analyzed the age at the amblyopia treatment, the type and degree of anisometropia, and the final visual acuity after treatment for visual improvement. Successful treatment was defined as a final visual acuity of 0.3 or more. Results: The mean age at treatment was 5.2 years. The types of anisometropia were hypermetropic in 14 patients, astigmatic in 4, and myopic in 3. The mean duration of treatment of amblyopia was 16 months, and 13 of 21 children with pure anisometropic amblyopia achieved 0.3 or better. The age at treatment was younger and the compliance rate was higher in the success group than in the failure group. Conclusions: The most common type was hyperopic in the pure anisomteropic amblyopia. Compliance and age at treatment were significantly related to best visual acuity attained in pure anisometropic amblyopia with unilateral visual acuity of 0.1 or worse. J Korean Ophthalmol Soc 49(6):973-978, 2008

가미생간탕(加味生肝湯)이 고지방식이로 유발된 지방간에 미치는 영향

최미영,우홍정,김영철,이장훈,Choi, Mi-Young,Woo, Hong-Jung,Kim, Young-Chul,Lee, Jang-Hoon 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.2

Objectives : The aim of this study was to investigate the effects of Gamisaenggan-tang on high fat diet induced nonalcoholic fatty liver disease. Methods: Rats were randomly divided into four groups. The Normal group was fed a solid diet containing 10% fat. The Gamisaenggan-tang (GS) group was fed a solid diet containing 10% fat and Gamisaenggan-tang (90mg/100g body weight). The Control group was fed a solid diet containing 60% fat. The HFD-Gamisaenggan-tang (HFD-GS) group was fed a solid diet containing 60% fat and Gamisaenggan-tang (90mg/100g body weight). Six weeks later, rats body weight, liver weight, serum ALT, GGT, ALP levels were measured. Histological findings (Oil red O staining), hepatic triglyceride, TNF-${\alpha}$, and TGF-${\beta}$ levels in the liver tissue were studied. Results: Average body weight of the HFD-GS group was significantly less than that of the Control group. There were no significant liver weight differences among each group. The GGT levels of the HFD-GS group were significantly less than those of the Control group. However, there were no significant differences in the ALT or ALP levels among the groups. TNF-${\alpha}$ protein production assessed by western blot analysis was reduced by Gamisaenggan-tang. Greater fat accumulation was observed in the liver tissue of the Control group than in the HFD-GS group, which means the Gamisaenggan-tang has an inhibitory effect on the accumulation of fat in the liver. Conclusion : The results suggest that Gamisaenggan-tang can be potential candidate for the treatment of nonalcoholic fatty liver disease in clinics.

인테리어 색채 인지 영역의 차이에 관한 실증적 실험 연구

최미영 ( Mi Young Choi ),박태욱(교신저자) ( Tae Wook Park ) 한국기초조형학회 2015 기초조형학연구 Vol.16 No.2

디자인 분야에서 색채는 심리적, 생리적 효용가치뿐만 아니라 커뮤니케이션 도구로서 시각 정보 전달이라는 중요한 역할을 한다. 본 연구는 시각적 색채 정보 전달방법에 대한 인테리어 디자인 분야에서의 활용도를 높이기 위해 실재감 높은 컬러 자극물과 색채 인지 영역을 측정하기 위한 도구를 개발하여 컬러계열별로 색채 인지 영역의 차이가 어떻게 나타나는지 정량적으로 비교하였다. 색채 인지 실험은 통제된 환경에서 실험 자극물에 대한 응답자들의 지각 반응을 유도하고 이를 측정하도록 설계되었다. 유효 표본 530명의 자료를 분석한 결과는 다음과 같다. 첫째, 응답자들이 선호하는 컬러계열은 ‘무채색 > 파랑 > 녹색 > 노랑 > 빨강 > 보라’순으로 나타났으며, 전체적인 톤에 대한 선호는 진한 톤에 비해 연한 톤을 선호하였다. 하지만, 컬러계열별로 비교해볼 때 선호하는 인테리어 색의 컬러 톤은 유의하게 차이가나는 것으로 분석되었다. 둘째, 인테리어 디자인을 통해 연상되는 컬러 이미지도 컬러계열별로 차이가 나타났다. 인테리어라는 구체적 자극물에 색이 적용되었기 때문에 개별 색이 가지는 고유의 감성 이미지 뿐 아니라 인테리어 컬러로서 깨끗함, 차분한, 자연스러운 이미지가 추구되고 있었다. 셋째, 먼셀 표색계의 채도와 명도의 변화정도를 고려한 인지 영역의 분포는 컬러계열별 자극물 컬러로 제시한 실제 컬러 값에 가깝게 수렴되는 정도와 색채 인지 편향의 차이를 보여주었다. 인테리어 분야에서의 색채는 공간 디자인 요소로서뿐 아니라 지각자와 상호작용 할 수 있는 개념으로 이해해야하기 때문에 물리적 계측치로서 정량적 접근이 아닌 지각자의 주관적 인지 특성을 반영한 인지심리학적 접근도 필요함을 주지해야 할 것이다. Color has important roles in design, not only psychological or physiological functions but also as communication tools for delivering visual information. To better apply color to interior design, this study desired to make experimental stimuli and develop an instrument to measure color cognition, and to get quantitative data on the difference between the areas of color cognition by colors. An empirical experiment was designed in order to induce and measure subjects’perceptual response about the differences among the areas of color cognition in a controlled environment. The data analysis of 530 valid samples and the results of this study were asfollows. First, respondents were categorized by interior color preferences; the preference order was neural > blue > green > yellow > red > purple. The overall preference for color tones was pale tone favored compared to the dark tone, but each preferencecolor tone in interior stimuli was significantly different by colors. Second, color images associated with interior design were totally different by colors. They delivered sensitivity of the color itself, but clean, calm, and natural images were commonly pursued with high preference as interior colors. Third, the distribution of color cognitive areas, considering degree of alteration of chroma and brightness in Munsell Color Specification System, showed the closely convergent degree to real exact color value used in experimental stimuli and the differences of color cognitive bias according to colors. Now, in interior design field, color research should not be analyzed physical terms and need to be analyzed in a cognitive psychology perspective reflecting the characteristic of subjects because color should be understood not only as a design element for space but also as a concept that can interact with subjects.

119구급대원들의 뇌졸중 환자에 대한 교육 및 지식수준이 주관적 인식수준에 미치는 영향

최미영(Mi-Young Choi) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.3

본 연구는 119구급대원들이 1년 동안 뇌졸중 환자에 대한 교육이 뇌졸중 지식 및 뇌졸중 환자처치에 대한 주관적 인식에 미치는 영향을 규명하기 위한 연구이다. 자료수집은 강원, 충청, 경북에 소재하는 소방공무원 중 119구급대원으로 활동하고 있는 남녀 196명을 대상으로 2018년 2월 17일부터 7월 31일까지 조사하였다. 수집된 데이터는 SPSS/PC+ 20.0을 사용하였으며, 빈도분석, t-test ANOVA 다중회귀분석을 하였다. 첫째, 119구급대원들의 일반적 특성이 뇌졸중 교육 및 전문교육 필요성이 뇌졸중에 대한 지식수준, 뇌졸중 환자처치에 대한 주관적 인식수준에 영향을 미치는 것으로 나타났다. 둘째, 119구급대원들의 1년 동안의 교육이 뇌졸중 지식수준과 뇌졸중 환자처치에 대한 주관적 인식수준에 영향을 미치는 것으로 나타났다. 셋째, 119구급대원들의 뇌졸중에 대한 지식수준이 뇌졸중 환자처치에 대한 주관적 인식수준에 영향을 미치는 것으로 나타났으며, 지식수준의 하위요인인 지식, 증상, 징후에 영향을 미치는 것으로 나타났다. 이 같은 결과로 볼 때 119구급대원들의 1년 동안 뇌졸중 환자처치 및 이송 교육은 뇌졸중 지식수준이 증가할수록 뇌졸중 환자처치에 대한 자신감, 처치 만족도, 수행 적절성에 대한 주관적 인식수준이 향상되는 것으로 나타났다. 따라서, 119구급대원들의 뇌졸중 환자에 대한 지식, 주관적 인식수준을 향상시키기 위해 주기적인 교육과 적절한 관리가 필요하다. This study examined the effect of 119 emergency medical technicians" education over one year on the stroke knowledge and subjective perception of stroke care. A survey was conducted from February 17 through July 31, 2018, on 196 men and women who worked as 119 emergency medical technicians in G-do, C-do, and the provinces. The collected data was analyzed by SPSS 20.0 version for Windows. The findings of the study were as follows. First, the general characteristics of the 119 emergency medical technicians were found to have affected their stroke education and their need for professional education, awareness levels of knowledge concerning stroke and their subjective awareness levels of stroke patient care. Second, one-year education for the 119 emergency medical technicians was found to have impacted on their stroke knowledge and subjective awareness levels concerning care for stroke patients. Third, the knowledge of the 119 emergency medical technicians on stroke was found to have influenced their subjective awareness levels of stroke patient care. Therefore, the results suggested that regular education and proper management to improve their knowledge on stroke and subjective awareness.

국어 수업을 통한 창조적 미래 인재 육성 방안 연구

최미영 ( Choi Mi-young ) 창조교육학회 2022 창조교육논총 Vol.24 No.-

For our students, middle school is the most important time of their lives. I believe that our students' future will be more meaningful and happy lives depending on what values they establish and continue to practice during this period. While conducting class conversations, emotional expression writing classes, reading discussion classes using textbooks, and convergence project classes, I saw students' lively class expressions, and I thought again that our teachers' mission is to lead and help our students create a happy life. Through the Korean language class conversation process, I wanted to present directions like a systematic and structured way of life to future talented students and guide them to be familiar with their minds and bodies. In other words, I constantly thought about how to foster creative future talent through Korean classes in the hope that it will create its own meaning in classes with students that are repeated every day and help them enter a bigger society and live as confident people in the future. Our students are the ones responsible for our future era. It is an important role of teachers to lead the direction of how to live for our students, who are future talents. There are so many words that define future talent. I think it is also an unreasonable expression to define the future talent in a few words. However, I believe that if children who have not yet established systematic thinking and direction are given specific words, it will help them grow through direction setting and practice. Writing of emotional expression for conversation and aesthetic enhancement in Korean class. In reading discussions using textbook works and curriculum convergence project classes, consistent classes and school life guidance were conducted to set future talents with personality, problem-solving skills, and creativity, and to continuously present their justification and direction. The intention was to create a healthy personality that smells like a person who benefits humans in a society where they live with others, a person who has the ability to solve any problem on his own, and a creative person who can make a life based on fresh and creative ideas about what he or she does in an anxious society. To that end, efforts were also made to connect with students' healthy lives by creating a practical stage that can continuously structure, internalize, and embody class conversations. It is class conversation, structuring, internalization, and physicalization, which are the four stages of class conversation.