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영역 분류를 이용한 다분광 화상데이터의 화소 단위 선형 예측 기법
조윤상,유승식 공주영상정보대학 2000 논문집 Vol.7 No.-
본 논문에서는 기존의 비선형 예측기에 비해 구현과정이 간단한 선형 예측기를 사용하였으며, 화상 데이터의 영역별 특성을 고려하기 위해 영역분류 기법을 도입하였다. 실험 결과를 통하여 알 수 있듯이 기존 방법에 비해 제안한 알고리즘에 의해 예측된 영상의 PSNR이 평균적으로 향상됨을 확인하였다. 영역 분류에 관한 연구가 좀 더 있으면 영역별 예측이 더 정확해 질 것이다.
조윤상,정석찬,유한상,김종만 한국동물위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.2
Tuberculin positive cattle without gross tubercle lesions should be confirmed by the bacteriological examination to determine the state of the infection. To overcome the time-consuming and laborious identification by culture and biochemical tests, polymerase chain reaction(PCR) has been used to identify Mycobacterium bovis. Due to various lipids in the cell wall of Mycobacterium spp, novel methods of DNA extraction from Mycobacterium spp have been developed. In this study, a newly developed guanidium isothiocyanate/silica DNA extraction method was directly applied to specimens from the tuberculin positive cattle. DNAs were directly extracted from the lymph nodes and the major polymorphic tandem repeat(MPTR) and mycobacterial protein of BCG 70(MPB70) were amplified using PCR. The DNA extraction method using guanidium isothiocyanate/silica was efficient and safe, and the MPTR and MPB70 primers were specific to M bovis. Therefore, MPTR and MPB70 PCRs will be useful for the detection of M bovis in the lymph node from skin-test positive cattle.
비교 피내 검사와 감마 인터페론 검사에 의한 우결핵의 진단
조윤상,김종만,정석찬,우승룡,김종염,유한상,박용호,안종삼,Cho, Yun-sang,Kim, Jong-man,Jung, Suk-chan,Woo, Seung-ryong,Kim, Jong-yeom,Yoo, Han-sang,Park, Yong-ho,Ahn, Jong-sam 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6
Since Robert Koch found tubercle bacilli in 1882, the studies on tubercle bacilli of human and animal had been carried out. Being old tuberculin(OT) introduced in 1890, the specificity of the diagnosis of tuberculosis has been improved by continual uses of heat concentrated synthetic medium(HCSM) and purified protein derivatives(PPD) tuberculin. Now, two types of tuberculin test are used worldwidly ; the single intradermal test(SIT) using bovine tuberculin and the single intradermal comparative tuberculin test(SICTT) using avian and bovine tuberculins. In the SICTT, each countries have used with different combination of both avian and bovine tuberculins' titers. However, this kinds of studies have not reported in Korea. Therefore, the studies on the combination of their tuberculins' titers were performed through intradermal test of guinea pigs sensitized with either Mycobacterium bovis or M avium and were examined in 10 cattles of SIT positive reactors. Also, IFN-${\gamma}$ assay, the latest diagnostic method of bovine tuberculosis, was experimentally applied to SIT positive reactors. For determining the optimal titers, sensitized guinea pigs with M bovis and M avium were intradermally injected avian and bovine tuberculin. In guinea pigs sensitized with M bovis, bovine tuberculin 50 T.U. showed significant difference from all tested concentrations of avian tuberculin(p < 0.05). In guinea pigs sensitized with M avium, there is significantly different between bovine tuberculin and avian tuberculin by 25 T.U.(p < 0.01). Therefore, optimal titers of bovine and avian PPD tuberculins' titers for the SICTT in Korea were 5,000 and 2,500 tuberculin units, respectively, and the swelling diffences between bovine and avian site in SIT positive reactors were above 3mm. Also, in IFN-${\gamma}$ assay, the 9 SIT positive reactors were showed all the positive reactions.