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황련(黃連)이 구강암 세포에서의 세포자멸사에 미치는 영향
이재근,박숙자,김상찬,지선영,Lee, Jae-Geun,Park, Sook-Jahr,Kim, Sang-Chan,Jee, Seon-Young 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.2
Objectives : The aim of this study was conducted that CRE (Coptidis Rhizoma Extract) induces apoptosis in YD-10B cells, human oral squamous carcinoma cell line. Methods : In this study, YD-10B cells were exposed to CRE (0.03-0.30 mg/ml), for 6-24 hours. We measured the effects of CRE on the changes of cell viability and cell membrane, TUNEL assay of CRE-treated YD-10B cell. Results : In this study, CRE caused a decrease of viability in YD-10B cells, human oral squamous carcinoma cell line. When YD-10B cells were treated with CRE, cells showed dose-dependent manner apoptotic cell death. Conclusions : These results suggest that CRE may be potential therapeutic approach in the clinical management of oral squamous cell carcinoma.
이재근,송해범,정선부,홍기창 ( J . K . Lee,H . B . Song,S . B . Jung,K . C . Hong ) 한국축산학회 1980 한국축산학회지 Vol.22 No.2
This experiment was carried out to determine the optimum amount of semen (sperm number) to use per injection in artificial insemination of hens. the experimental birds used were 240 hens of White Plymouth Rock inbred line and 20 cocks of Australorp pure line at 7 months of age. The four treatments compared were: 0.005 ㎖ or 16 to 18 million sperm per insemination (T1), 0.01 ㎖ or 32 to 36 million sperm (T2), 0.02㎖ or 64 to 72 million sperm (T3), and 0.03 ㎖ or 96 to 108 million sperm (T4). thirty birds were allotted per treatment within a replication, and there were two replications in the experiment. Ringer`s Solution were used for diluent of semen(1:3), and semen was inseminated within 30 minutes after collection. The hens were inseminated 4 times with the intervals of 4 days. The results obtained are summarized as follows. 1. The fertility for four days after the second insemination was 46.4% for the T1, 72.9% for T2, 73.3% for the T3 and 86.3% for the T4. The fertility of the T1 was significantly lower than the others, but the differences among T2, T3 and T4 were not significant. These results agree with the previous report (1978). 2. The fertility for 4 days after the 3rd insemination was 71.0% for the T1, 73.4% for the T2, 73.3% for the T3 and 77.1% for the T4, and t he fertility after the 4th insemination was 70.4% for the T1, 65.7% for the T2, 69.2% for the T3 and 79.3% for the T4. None of the differences among the treatments were statistically significant in each ease. It was concluded that the insemination of 0,005㎖ (16 to 18 million sperm per insemination) can attain the same level of fertility as the insemination of 0.01 ㎖ to 0.03㎖ after the 3rd or 4th insemination. 3. In view of the results obtained in this study, the least optimum dose of semen in the case of 4 days interval was estimated to be within the range from 0,005㎖ to 0.023㎖
1,2,4-Triazine III : 1,2,4-Triazine 유도체의 Methiodide 염 합성과 이들 염의 고리 축소화반응에 의한 1,2,4-Triazole 유도체 합성
이재근,유향선,Jae Keun Lee,Ryu Hyang Sun,W. W. Paudler 대한화학회 1989 대한화학회지 Vol.33 No.4
1,2,4-Triazine 유도체들을 acetone 용매하에서 methyl iodide와 반응시켜 다양한 1-methyl-1,2,4-triazinium iodide 염을 합성하였다. 또한 이들 염을 10% NaOH 수용액 내에서 $K_3Fe(CN)_6$로 산화시킨 결과 고리 축소화반응이 일어나 1-methyl-1,2,4-triazole 유도체를 주로 얻을 수 있었으나 $C_6$에 치환기가 없을 경우는 미량이긴 하지만 1,6-dihydro-6-oxo-1,2,4-triazine 유도체도 동시에 얻을 수 있었다. 고리 축소화반응은 $OH^-$에 의해 먼저 pseudo base를 형성하고 이 Pseudo base가 1-methyl-1,2,4-triazole과 1,6-dihydro-6-oxo-1,2,4-triazine으로 진행됨을 확인할 수 있었고 또한 1,2,4-triazine의 3개의 질소원자 중 $N_1$ 원자에 quaternization됨을 확인할 수 있었다. Various 1-methyl-1,2,4-triazinium iodides were easily synthesized by the reaction of various 1,2,4-triazines and methyl iodide in acetone. When methyl iodide salts of 1,2,4-triazine derivatives were treated with $K_3Fd(CN)_6$ in 10% NaOH solution, 1-methyl-1,2,4-triazole derivatives as the main products were obtained by ring contraction. In addition to 1-methyl-1,2,4-triazole derivatives, 1,6-dihydro-6-oxo-1,2,4-triazine derivatives as the minor products were also obtained when there were no substitutents in $C_6$-position of 1,2,4-triazines. The formation of pseudo base by $OH^-$ and then oxidation to either 1,6-dihydro-6-oxo-1,2,4-triazines or 1-methyl-1,2,4-triazoles were suggested as the mechanism of the ring contraction. This mechanism also verified that the position of quaternization was neither $N_2$ nor $N_4$ but 1-nitrogen of 1,2,4-triazine.