임신토 생식기관의 변화에 관한 조직학적 연구 1 . 종의 변화에 관하여

송해범,이재근,김상균 ( H . B . Song,J . K . Lee,S . K . Kim ) 한국축산학회 1976 한국축산학회지 Vol.18 No.6

This experiment has been carried out to study histological changes of vaginal epithelial cells in rabbits during pregnancy, using 26 heads of 8 month-old does. Vaginal smears were collected from vaginal lumen before copulation and on the 7th day of pregnancy. Tissue samples were collected from vagina and vaginal vestibule before copulation and on the 7th, 14th, 21st, and 28th day of pregnancy. The results observed were as follows. 1. Vaginal vestibule epithelium shoved considerable decrease is the number of cell layers from 5-15 during progestational phase to 1 or 2 layers on the 28th day of pregnancy. Vaginal epithelium of 1-3 layers remained constant throughout pregnancy. 2. Vaginal vestibule epithelial cells were changed from squamous, circular and columnar type of non-pregnancy into squamous and cuboidal or columnar typer of pregnancy, while epithelial cells were changed from cuboidal or columnar type into squamous or columnar type. 3. In vaginal smears, cornified cells were found in the progestational period, but they were no longer observed on the 7th day of pregnancy. In non-pregnancy, cornification-sloughing was observed in vaginal epithelium; and also mitotic figure in qasal layer, both of these were observed scarcely any after the 7th day of pregnancy. 4. Epithelial ridges showed regular figure in non-pregnancy, but the size of them were not constant after the 7th day of pregnancy. The number of them began to decrease only after the 14th day and showed apparent decrease on the 28th day of pregnancy. 5. Vaginal wall began to enlarge from the 14th day, especially after the 21st day of pregnancy. Lamina propria was outstandingly atrophied after the 21st day of pregnancy. 6. It is considered that pregnancy diagnosis would be possible within 1 week of pregnancy by observing the increasing trend of viginal mucus and the decreasing trend of cornified cells in rabbit.

한국재래염소의 번식능력에 관한 연구

송해범,이재익,김주환,공건오 대구대학교 과학기술연구소 2000 科學技術硏究 Vol.7 No.1

These studies were conducted to examine the reproductive performance of the Korean native goats ( KNG) . The results of this study were as follows 1. The first estrus was 141.24±18.17 days after birth in the KNG. 2. The length of estrous cycle was 20.58±2.63 days in the KNG. 3. The duration of estrus was 17.83±7.34 hours in the KNG. 4. The gestation length was 150.69±6.14 days in the KNG. 5. The birth weights by kidding type were 2.28±0.26Kg. 2.11±0.30 Kg and 1.64±0.19 Kg in single, twin and triplet over, respectively. The birth weight by sex were 2.09±0.33Kg and 2.09±0.37Kg in male and female, respectively, and the mean birth weingt was 2.04±0.34Kg in the KNG. 6. The differences of weight by kidding type were 5.68±0.29Kg. 7.49±0.39kg and 11.71±1.36Kg in single, twin and triplet over before and after parturition respectively, and the average was 8.00±0.59Kg in the KNG. 7. The age of first kidding was 10∼12 months, the optimum age for first breeding was over 6 months in the KNG. 8. Korean native goats were the continuous breeder.

생식선자극(生殖腺刺戟) 호르몬 처리가 산양의 배란난자(排卵卵子) 및 난포란(卵胞卵) 채취에 미치는 영향

송해범,입곡명 한국낙농학회 1986 韓國酪農學會誌 Vol.8 No.4

本 硏究는 成熱 및 未成熱 雌山羊에 生植腺剌載호르몬 처리가 排卵卵子 및 卵胞卵 채취에 미치는 영향을 검토하기 위하여 18 頭의 成熱雌山羊과 21 頭의 未成熱雌山羊을 公試하여 실시하였다. 實驗結果를 要約하면 다음과 같다. 1. 穎粒膜細胞에 둘러 쌓인 卵胞卵은 未成熱雌山羊에서 호르몬 무처리구 22.7개, PMSG처리구 54.2개, PMSG와 HCG 처리구 25.8개였으며, 成熱雌山羊에서는 PMSG 처리구 16.3개, PMSG와 HCG 처리구 7.8개였다. 2. 擴散된 穎粒膜細胞에 둘러 쌓인 卵胞卵은 PMSG와 HCG 처리구에서 成熱雌山羊은 1.7개, 未成熱雌山羊은 8.0개로 未成熱雌山羊이 成熱雌山羊보다 生植腺剌載호르몬 처리에 더욱 민감한 반응을 보였다. 3. PMSG와 HCG 처리구에서 排卵點은 成熱雌山羊은 7.4개, 未成熱雌山羊은 3.9개였으며, 採卵率은 각각 57%와 51%였다.

山羊의 體外受精에서 受精能獲得精子의 卵胞卵에의 侵入時期에 관한 硏究

송해범,고태혁,변태호,심금섭 대구대학교 농업과학연구소 1988 農業科學硏究論文集 Vol.2 No.-

山羊에 있어서 顆粒膜細胞에 둘러 쌓인 卵胞卵을 體外培養한 후 m-KRB液에서 6시간 前培養한 精巢上體精子와 體外受精하여 6, 12, 18 및 24시간 檢鏡후 한 결과를 要約하면 다음과 같다. 授精후 6~24시간 精子와 追加培養된 卵胞卵은 68% (59%~73%)가 第2成熟分裂中期까지 成熟되었다. 6시간 후에 卵胞卵은 전혀 受精되지 않았으나 12시간 후에는 26%, 18시간후에는 44% 및 24시간 후에는 61%가 각각 受精되었다. 또한 受精된 卵胞卵은 12시간 후에 전부 膨化된 精子頭部를 갖고 있었으나, 18시간 후에는 17%로 감소되었고 83%는 雄性前核으로 발달되었고, 24시간 후에는 전부 雄性前核으로 발달되었다. 이상의 결과로 보아 山羊의 體外受精에서는 卵胞卵에 精子의 侵入이 授精후 적어도 12시간 이전에 일어났을 것으로 추측된다.

햄스터 적출자궁에서 전배양한 소 정자의 난포란에의 침입시기 및 체외수정 초기상에 관한 연구

송해범,김광식 한국동물생명공학회(구 한국동물번식학회) 1992 Reproductive & developmental biology Vol.16 No.3

體外培養한 소卵胞卵과 햄스터摘出子宮에서 前培養한 소 精子의 體外受精에 관한 硏究

宋海範 한국동물생명공학회(구 한국동물번식학회) 1989 Reproductive & developmental biology Vol.13 No.1

受精能獲得精子와 山羊卵子의 體外受精에 관한 硏究

송해범,심금섭 대구대학교 농업과학연구소 1987 農業科學硏究論文集 Vol.1 No.-

Ejaculated and/or epididymal spermatozoa of goats were preincubated for 4-6 h in the isolated genital tracts from hamsters, gilts and goats, or for 3-8 h in m-KRB solution. After preincubation, they inseminated the goat ovulated eggs collected shortly after ovulation and/or the follicular oocytes with intact and without cumulus cells cultured for 25 h in m-KRB solution, and the oocytes with dispersed cumulus cells which were not cultured. The results obtained were as follows. (1) None of the ovulated eggs were fertilized after insemination with ejaculated and epididymal spermatozoa preincubated for 4-5 h in the isolated genital tracts from hamsters, gilts and goats. But 50 and 38% of the ovulated eggs were fertilized with epididymal sermatozoa preincubated for 5 and 6 h in m-KRB solution, respectively. (2) None of the follicular oocytes were fertilized after insemination with ejaculated spermatozoa preincubated for 4-6 h in the uteri isolated from hamsters and goats, and with epididymal spermatozoa preincubated for 4-6 h in the isolated hamster uterus. But 38% of the follicular oocytes were fertilized with epididymal spermatozoa preincubated for 5 h in the isolated goat uterus. (3) After further culture with spermatozoa for 18-24 h, 50-89, 89 and 14-33% of the oocytes with intact, with dispersed and without cumulus cells had matured to the second metaphase, respectively. (4) When epididymal spermatozoa were preincubated for 5 h at the concentration of 4.2x10^(8)/ml and 6 h at the concentration of 3.5x10^(8)/ml in m-KRB solution, 36 and 33% of the oocytes with intact cumulus cells were fertilized. (5) The results suggested that epididymal spermatozoa can be capacitated in m-KRB solution, and that the follicular oocytes matured in culture could be used for the study of fertilization in vitro instead of the ovulated eggs.

난관체류시간에 따른 생쥐초기배의 체외발생능력

송해범,서병부,김광식,박성은,이상호,Song, H.B.,Seo, B.B.,Kim, K.S.,Park, S.E.,Lee, S.H. 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.2

Development in vitro of 2-cell mouse embryos was examined after appropriate exposure to oviductal milieu to demonstrate biological activity present in the oviducts. ICR and ($C57Bl/6{\times}Balb/c$) $F_1$ hybrid mice were superovulated and mated for the recovery of early embryos. Embryos were recoverd at every 2h intervals from 32h post-hCG(hph) to 56 hph. The proportions of developmental stages were determined in the recovered embryos. Development in vitro of 2-cell embryos was more rapid in $F_1$ hybrid than in ICR, showing high proportions of 4-cell embryo and blastocyst at 120 hph. 100% of blastocyst development was obtained at 38hph in $F_1$ hybrid and at 50 hph in ICR when 2-cell embryos were cultured upto 120hph in vitro. Moreover, in vitro culture of oviducts containing 2-cell embryos in ICR mice for 12h from 34hph to 46hph increased developmental capacity of ICR mouse embryo in vitro. The results indicate that oviductal environment contains substances having mitogenic activity and overcoming early cell block in vitro. The mitogenic activity is effective in vitro as well as in vivo.

돼지난포란의 난구세포 팽화는 과립막세포에 의해 억제된다

송해범,박수봉 한국축산학회 1994 한국축산학회지 Vol.36 No.5

We tested the current popular hypothesis that sulfated glycosaminoglycans (GAGS) in murine follicular fluid may inhibit the precocious expansion of the cumulus oophorus in the antral follicle before the LH surge. Using porcine follicular oocytes, the effect of GAGS as a form of follicular fluid was examined in the cumulus exapansion. Degree of expansion of the cumulus-oocyte complexes cultured in porcine follicular fluid was greater then that in medium supplemented with 10% fBS and 0.02 Au/㎖ FSH. However, the cumulus oophorus of native oocytes tightly connected with mural granulosa cells in hemi-sec ted follicles remained at intact cumulus oophorus after incubation in the above conditions. In contrast to the results in mice, heparin could not inhibit cumulus expansion in pig, despite of higher concentrations (range: 100㎍∼10㎎/㎖) than those of total GAGS in porcine follicular fluid. The results indicate that GAGS may not be a main factor in regulation of cumulus oophotus during follicular development in pig, and the maintenance of tight cumulus oophorus may be due to a factors) from mural granulosa cells and contact between membrana granulosa and cumulus cells.

염소, 번식능력 향상에 관한 4가지 원칙

송해범 한국단미사료협회 2005 피드저널 Vol.3 No.5