아이빔테크놀로지(주), 미래 바이오헬스 시장의 첨단 플랫폼 생체현미경 솔루션 글로벌 선도기업을 노린다

김필한 한국화학공학회 2021 NICE Vol.39 No.2

동적 채널 Add/drop 환경에서 광섬유라만증폭기의 이득제어알고리즘

김필한,박남규 한국통신학회 2004 한국통신학회 기타 간행물 Vol.2004 No.11

Intravital laser-scanning microscopy for real-time cellular visualization

김필한 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

Recent advances in genomic technology have allowed a creation of animal model for human disease with genetically encoded biomarkers such as green fluorescent protein (GFP), which has opened up a new avenue to investigate complex pathophysiology of human disease in much greater details at cellular and molecular level. Over the recent years, intravital laser-scanning microscopy has demonstrated dynamic 3D visualization of various biological processes in the living subject, which provides unprecedented insights those were impossible to obtain by traditional static 2D snapshots (e.g. histopathology and cytometry). It has been utilized to monitor gene expression, protein activity, drug delivery, cell trafficking, cell interaction, physiological response under external stimuli in live animal in vivo, which provides new insights unobtainable by conventional ex vivo and in vitro observation. In this talk, recent in vivo cellular imaging studies utilizing custom-design ultrafast laser-scanning intravital microscopy system will be introduced. First, in vivo visualization of T and B lymphocyte trafficking at the high endothelial venule (HEV) of lymph node will be demonstrated. Individual endothelial cell of HEV can be clearly identified with its distinctive cuboidal morphology. Dynamic flowing behaviors of T and B lymphocytes and their dynamic migrations across endothelial cells and fibroblastic reticular cells were analyzed in vivo. Second, in vivo monitoring of small lipid and drug molecules in intestinal villi will be described. By utilizing lacteal-reporter (Prox-1-GFP) mouse, we successfully visualized transepithelial absorption of molecules across villus enterocyte, diffusion through lamina propria and subsequent transport via lacteal. Interestingly, we observed active contractile movement of lacteal in concert with villi motion, which suggests lacteal may act as an active pump during lipid absorption, not merely as a passive conduit. Moreover, we identified highly diverse pattern in absorption dynamics of various exogenous molecules as well as intrinsically fluorescent drugs through enterocytes and lacteal in vivo.

의생명연구를 위한 생체 내 현미경

김필한,Kim, Pil-Han 한국광학회 2013 광학과 기술 Vol.17 No.3

아이빔테크놀로지㈜, NIR Femto-second Laser를 이용한 세계 최소형 3차원 올인원 이광자 생체현미경

김필한 한국레이저가공학회 2022 Laser Solutions Vol.25 No.8

Effects of suspended solids in natural seawater on microalgal biomass productivity

김필한,박한울,이철균 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1

Suspended solids, with various sizes, in seawater, may affect algal biomass productivity or not. In the previous research, biomass productivity of microalgae was enhanced by filtering all SSs using microfiltration. In this research, the fractionated SSs by their sizes were tested to find the effect of each fraction on the growth of a microalga, Tetraselmis sp. KCTC12433BP. The SSs were fractionated by filtration with glass microfiber filters, which pore sizes were 0.2 μm (A), 0.7 μm (B), 1.2 μm (C), and 3.0 μm (D). Each fractions were put into the f/2-Si medium with natural seawater. The highest overall biomass productivity, 0.10 g/L/day, was obtained from D fraction group, and the cultures with B, C, and A fraction groups followed after. In the control group, containing all SSs, the biomass productivity was decreased by 61%. Some suspended solids, the size ranges from 1.2 μm to 3.0 μm, look as if they have advantageous effect, while the other SSs that larger than 3.0 μm look as if they have disadvantageous effect on the microalgal growth. Further studies will be on analyzing the constituents.

공공봉사동기와 이직의도: 정서적 몰입과 직무만족의 매개효과를 중심으로

김필,정윤진,임도빈 서울대학교 한국행정연구소 2020 行政論叢 Vol.58 No.1

The main purpose of this paper is to identify the missing link between public service motivation (PSM) and intention to leave the job among central government employees. Specifically, this paper hypothesizes that affective commitment and job satisfaction have mediating effects on the relationship between PSM and turnover intention. Affective commitment is closely related to the process of internalizing organizational values; therefore, it is expected that individuals with higher PSM are likely to dedicate themselves to public organizations. In addition, individuals tend to positively assess their organization when they have a strong sense of belonging; that is, affective commitment is likely to affect job satisfaction. According to previous research, moreover, it is widely known that employee’ job satisfaction has a negative relationship with turnover intention. To examine these hypotheses, partial least squares structural equation modelling (PLS-SEM) was adopted. Results reveal that PSM has slightly positive impact on turnover intention but has the most significant and negative influence through the double mediation of affective commitment and job satisfaction. This implies that PSM and turnover hypothesis based on person-organization fit might need to be reconsidered. 본 논문은 공조직에 특수한 동기로서 공공봉사동기가 어떤 경로를 거쳐 공무원의 이직 의도에 영향을 미치는가를 연구하는 데 목적이 있다. 중앙부처 공무원을 대상으로 공공봉사동기, 조직동일시, 직무만족이 공무원의 이직의도에 미치는 영향에 대하여 PLS 구조방정식을 통해 검증하였다. 분석 결과 공공봉사동기가 이직의도에 미치는 직접적인 영향력은 미약한 수준에서 정(+)의 방향으로 나타났으며 이는 해당 관계에 대하여 기존 개인-조직 정합성에 기초한 접근이 제고되어야 함을 시사한다. 그러나 정서적 몰입과 직무만족의 이중매개를 거칠 때 이직 의도에 부(-)의 영향력을 미치며, 경로의 효과도 가장 큰 것으로 나타났다.

중형 기공성 알루미나의 제조와 응용

김필,주지봉,김우영,김종식,김희수,김영훈,송인규,이종협 한국공업화학회 2006 한국공업화학회 연구논문 초록집 Vol.2006 No.-

Legitimacy as Resource: How Did the Presidential Impeachment in South Korea Succeed?

김필,임도빈 한국조직학회 2022 한국조직학회보 Vol.19 No.2

This study retrospectively examines how the South Korean government tackled the “2016 Candlelight Protest” which ended with the impeachment of the then-President Park Geun-hye. To understand the rally’s impact on impeachment decision, we trace strategies of the Blue House and a civil organization named “Park Geun-hye Resignation Nationwide Movement.” Resource dependence theory has been adopted to explain the shift from one stage to another, in a sequence of four critical turning points of the process: 1) the outset of the scandal, 2) the third candlelight protest rally, 3) impeachment vote, and 4) the verdict of the Constitutional Court. The key finding is that when two conflicting forces collide multiple times in different stages, their chosen strategies in accumulation determine the end resulting equilibrium point. “Compliance” and “management” strategies, which are more extreme ways for an organization to cope with the state of affairs, work more effectively than “avoidance” strategy.

증거기반정책을 위한 지방행정 통계지표의 현황분석과 새로운 지표 제안

김필,전대욱,이경은,주희진,김해솔 (재) 한국지방행정연구원 2023 地方行政硏究 Vol.37 No.2

The central government and local governments have been striving to establish a stronger connection between statistics and policies in order to achieve evidence-based policies. However, from the perspective of local autonomy and decentralization, local statistics still lack in quantity, and some fall short of important values that characterize evidence-based policy management. This paper categorizes local statistics into three decentralization powers (legislative, organizational, and administrative powers) and conducts a survey of experts to determine the reliability, accessibility, and utility of existing statistical indicators and platforms. New indicators are proposed that focus on necessity, measurability, and usability. It is expected that local statistics will function properly as primary data or direct policy evidence to derive evidence-based policy management. 중앙정부와 지방자치단체는 증거기반정책의 실현을 위해 통계 기반 정책 관리 제도 등을 통해 과학적 정책 수립 체계를 구축하고 통계와 정책의 연계를 강화하고자 노력해왔다. 하지만 여전히 지방자치단체의 지표 생산은 자치분권의 관점에서 보았을 때 양적으로 열악하며, 증거기반정책에서 중시하는 증거의 신뢰성 등 여러 가치에 부합하지 않는 측면이 있다. 본 논문은 지방행정 및 자치분권 제도 관련 통계지표를 자치행정권, 자치조직권(인사관리・집행기관), 자치입법권으로 분류하고 각 분야 전문가들의 인식조사를 수행하여 기존 통계지표와 통계플랫폼의 신뢰성・접근성・지표활용도를 분석하고 필요성・측정가능성・활용가능성을 중심으로 새로운 지표를 제안하였다. 향후 과학적 방법론에 입각한 정책증거를 도출하기 위한 1차 자료 혹은 직접적인 정책 증거로서 지방통계가 적절하게 기능하길 기대한다.