Xylan 대사 유전자를 가진 미니 효모인공염색체의 가공 및 Mitotic Stability 분석

강다인,김연희 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.3

In this study, yeast artificial chromosome Insert (YAC) harboring genes which related xylan metabolism was constructed by using chromosome manipulation technique. For efficient chromosome manipulation, each splitting fragment (DNA module) required for splitting process was prepared and these DNA modules were transformed into Saccharomyces cerevisiae strain YKY164. By two-rounds chromosome splitting, yeast chromosome VII (1,124 kb) was split 887 kb-YAC, 45 kb-mini YAC and 198 kb-YAC and YKY183 strain containing 18 chromosomes was constructed. Splitting efficiency for chromosome manipulation was 50- 78% and expression level of foreign genes on 45 kb-mini YAC and enzyme activity were indistinguishable from that of the YKY164 strain. Furthermore, xylan-degraded products by recombinant enzymes were confirmed and mini-yeast artificial chromosome maintained stable mitotic stability without chromosome loss during 160 generations. 본 연구에서는 염색체가공기술을 이용하여 xylan으로부터다양한 대사산물을 생산할 수 있는 유전자를 도입한 효모인공염색체를 구축하였다. 효율적인 염색체가공기술인 PCS법을 이용하기 위해 염색체 splitting에 필요한 splitting fragment (DNA module)를 각각 제작하였고, xylan 대사에관여하는 유전자군을 가진 YKY164 균주에 형질전환하였다. 두번의 염색체 splitting에 의해 1,124 kb의 효모 7번염색체는887 kb-YAC, 45 kb-mini YAC와 198 kb-YAC로 가공되었으며, 총 18개의 염색체를 가진 YKY183 균주를 구축하였다. 염색체가공을 위한 splitting efficiency는 50−78%였으며, 45 kb-mini YAC 상에 있는 외래유전자의 발현 및 효소활성은 염색체가공 전과 비교하여 유의미한 변화 및 저하는 관찰되지 않았다. 또한 생산된 재조합효소에 의한 xylan의 분해산물을 확인하였으며, 160 generation 동안 미니 효모인공염색체는 염색체의 결실없이 안정적인 mitotic stability를유지하였다.

전국 미세먼지 취약지역 도출을 통한 중점관리지역 분석

강다인,김성훈,김장수,박찬,권혁수 한국사진지리학회 2022 한국사진지리학회지 Vol.32 No.4

This study intends to suggest a method for analyzing the vulnerability of fine dust nationwide by considering spatial information on fine dust and recent policy trends. As a result of national adsorption analysis, it was highly distributed in areas with many forests or green areas. Most of the areas with high vulnerability due to low adsorption amount were analyzed to be metropolitan areas and metropolitan cities with high population density and low amount of green space. A key management area was derived through spatial analysis of adsorption amount, concentration, and emission. Areas classified as very vulnerable areas seem to have to come up with policy measures within the local government, and management should be prioritized. Areas of moderate vulnerability and low vulnerability require constant monitoring and continuous maintenance of green areas. It is meaningful in that it analyzes the vulnerability of fine dust in the country in terms of risks to human health and the physical aspects that the region can accept, and has the advantage of intuitively exploring areas with insufficient capacity compared to the amount of fine dust. Since the vulnerability of fine dust was analyzed nationwide, it is highly useful as basic data in the policy decision-making process.

도시 내 열환경 분석에서 무인항공기의 활용가능성

강다인,문호경,성선용,차재규 한국조경학회 2018 韓國造景學會誌 Vol.46 No.2

Urban heat islands occur due to increases in the extent of artificial surfaces such as concrete, asphalt and high-risebuildings. In this regard, research into the use of satellite thermal infrared images for thermal environment analysis ofurban areas is being carried out. However, such analysis of the characteristics of individual land cover with low-resolutionsatellite images suffers from limitations because land cover patterns in urban areas are complicated. Recently, UAV hasbeen widely used, which can compensate for this limitation as it is able to acquire high-resolution images. In this paper,the accuracy of UAV infrared images is verified and the applicability of UAV in urban thermal environment analysis isexamined by comparing the results with land surface temperatures from Landsat 8 thermal images. The results show ahigh positive correlation of temperature values at 0.95, and no statistically significant difference between the two groups. Comparisons of land surface temperature according to land cover showed that the largest difference observed was 4.63℃in the Used area, and UAV images with small cell units reflected various surface temperatures. Furthermore, it was possibleto analyze the surface temperatures of various green spaces such as wetlands and street tree areas, which can lower surfacetemperatures in urban areas, with street tree shadows reducing surface temperatures by about 4-6℃. UAV can easily andrapidly measure the surface temperature of urban areas and is able to analyze various types of green spaces. Thus, thisis an effective tool for thermal environment analysis in urban areas to aid in the design or management of urban greenspaces, as it can allow for land cover and the effects of the various green spaces. 도시는 콘크리트, 아스팔트 등 인공적인 피복과 고층 건물의 증가로 인해 열섬현상이 발생되고 있다. 이와 관련하여 인공위성 열적외 영상을 활용한 도시의 열환경 분석에 대한 연구가 활발히 진행되고 있다. 그러나 복잡한 토지피복을가진 도시의 열환경 특성을 분석하기에는 위성영상의 공간해상도는 한계를 가지고 있다. 무인항공기(UAV)는 높은 해상도의 영상을 취득할 수 있어 이러한 한계점을 보완하기에 적절하며, 최근 폭넓게 활용되고 있다. 이에 본 연구는 무인항공기열적외 영상의 정확성 검증과 Landsat 8 열적외 영상과의 해상도 비교를 통한 도시 내 열환경 분석에서 활용가능성에대해 검토하였다. 정확성 검증 결과, 온도 값의 상관성은 0.95로 높은 양의 상관관계를 보였고, 두 집단의 통계적 차이가없었다. 해상도 비교를 위한 두 영상의 지표온도의 차이 분석 결과, 시가화 지역이 4.63℃로 가장 높은 차이를 보였으며,셀 단위가 작은 무인항공기영상이 다양한 지표온도를 반영하였다. 온도 그래프 비교 결과, 도시의 지표온도를 낮출 수있는 습지, 가로녹지 등의 세부적인 공간의 지표온도 분석이 가능하였고, 가로수 그림자의 지표온도저감 효과 분석 결과,약 4~6℃ 가량 낮추는 것으로 나타났다. 무인항공기는 세부적인 공간의 분석이 가능하였고, 단시간에 일정 면적의 지표온도를 취득하기 용이하였다. 따라서 무인항공기는 피복을 고려한 공원의 조성이나 관리, 소규모 녹지의 효과 분석 등도시 내 열환경 분석 전반에 걸쳐 높은 활용성을 가질 것이라 판단된다.

생태⋅자연도 등급변화에 영향을 미치는 요인 분석 -경상남도 일대를 대상으로-

강다인,이정현,윤혜연,박현수 한국사진지리학회 2023 한국사진지리학회지 Vol.33 No.3

본 연구에서는 2021년 3월 생태⋅자연도와 2023년 5월 생태⋅자연도를 활용하여, 경상남도 일대의 시기별 등급변화 양상과 등급변화 요인을 분석하였다. 분석 결과, 연구지역의 생태⋅자연도 면적은 2021년에 비해 2023년에 1등급 권역 1.7% 감소, 2등급 권역 0.5% 증가, 3등급 권역 1.1% 증가, 별도관리지역은 0.2% 증가하였다. 생태⋅자연도 등급 상향 요인은 식생 분야 조사의 정밀도 향상, 멸종위기 야생생물의 조사기법 고도화 및 조사지점 증가, 습지 조사 인벤토리 증가 및 서식처 질 향상, 지형조사 자료 축적 등인 것으로 나타났다. 그중에서도 최신장비 도입 등을 통한 조사기법개선으로, 과거 미발견된 생태⋅자연도 1등급 권역 평가 요소의 발굴이 등급 상향 요인으로 작용하였다. 한편, 생태⋅자연도 등급 하락 요인은 생태⋅자연도 작성지침 개정, 최신 토지피복지도 및 임상도 반영으로 인한 산림 및 습지 경계조정, 벌채지 증가, 멸종위기 야생생물 DB 갱신 등으로 나타났다. 도출된 결과는 국제적 실천 목표에 따른 보호지역 확대 및 유지의 기초자료로 활용될 수 있고, 생태⋅자연도 작성 및 활용에 관한 개선방안 수립 등에 도움이 될 것으로 기대된다. In this study, we used the ecology and nature map of March 2021 and the ecology and nature map of May 2023 to analyze the pattern of grade change and factors of grade change by period in the Gyeongsangnam-do area. As a result of the analysis, the area of ecology and nature map in the study area decreased by 1.7% in the 1st grade area, increased by 0.5% in the 2nd grade area, increased by 1.1% in the 3rd grade area, and increased by 0.2% in the separately managed zones in 2023 compared to 2021. The factors contributing to the increase in ecology and nature map ratings include improved precision in vegetation surveys, advancement of survey techniques for endangered wildlife, and increased survey points. In addition, it was found that wetland survey data increased, habitat quality improved, and landform survey data was accumulated. Among them, the improvement of survey techniques through the introduction of the latest equipment and the discovery of previously undiscovered ecology and nature map 1st grade area evaluation factors served as a factor in raising the grade. Meanwhile, the factors that led to the decline in the ecology and nature map grades were revision of the ecology and nature map drawing guidelines, update of the endangered wildlife database, adjustment of forest and wetland boundaries due to reflection of the latest land cover map and forest type map, and increase in deforestation areas. The derived results can be used as basic data for expanding and maintaining protected areas in accordance with international action goals, and are expected to be helpful in establishing improvement plans for the creation and use of ecological and nature maps.

시민참여 전국자연환경조사 데이터의 특성

강다인,박규령,Choi Seung Se,이태우 (사)지오에이아이데이터학회 2023 GEO DATA Vol.5 No.4

Citizen participation data has different characteristics from expert. Understanding the unique attributes of citizen participation data and considering the disparities between such data and that collected by experts is of paramount importance to harness citizen-generated data effectively. In this regard, location information was presented for five taxa of plants, insects, birds, amphibians, and mammals nationwide surveyed by citizens in 2022, excluding endangered species. Additionally, species information and administrative district information are included. It is anticipated that the integration of citizen participation survey results with expert research findings will enhance the efficacy of natural ecological monitoring. By directly collecting ecological data, which becomes the basic data for natural environment policy, opportunities for public participation in natural environment policy will be expanded, and through this, it will be possible to raise awareness of natural environment conservation.

고위험 임부의 태교실천, 자존감 및 사회적 지지가 모아애착에 영향을 미치는가?: 횡단적 조사 연구

강다인,박은아 한국여성건강간호학회 2022 여성건강간호학회지 Vol.28 No.4

Purpose: The incidence of high-risk pregnancies is increasing in Korea as the birth age increases due to late marriage. Maternal-fetal attachment is an important factor that affects children even after childbirth, but it is difficult for high-risk pregnant women to form maternal-fetal attachment. The current study aimed to explore whether taegyo practice (i.e., pregnant women’s efforts for fetal good growth and development), self-esteem, and social support influenced the degree of maternal-fetal attachment in women with high-risk pregnancies.Methods: The participants included 226 pregnant Korean women at ≥20 gestational weeks, hospitalized with 15 high-risk pregnancy conditions as defined by the Ministry of Health and Welfare. Recruitment via convenience sampling was done at four sites in Busan, Korea. Surveys were distributed and collected from February 1 to 28, 2022. Data analysis was conducted using descriptive statistics, the t-test, one-factor analysis of variance, Pearson correlation coefficients, and hierarchical multiple regression. Results: On average, participants were 33.97±4.23 years of age and at 31.65±6.23 gestational weeks. Preterm labor (35.4%) and gestational diabetes (21.0%) were the most common high-risk conditions. Maternal-fetal attachment was positively correlated with taegyo practice (r=.70, p<.001), self-esteem (r=.53, p<.001), and social support (r=.53, p<.001), all with statistical significance. Taegyo practice (β=.50, p<.001) and social support (β=.17, p=.030) explained 53% of variance in maternal-fetal attachment in women with high-risk pregnancies.Conclusion: Nurses caring for women with high-risk pregnancies during hospitalization can use these findings by promoting taegyo practice and enhancing social support to increase maternal-fetal attachment.

특별프로그램 : 대학생프로젝트 경진대회-포스터 발표 ; 무인 발권기 개선 Project

강다와,여명시 대한산업공학회 2010 대한산업공학회 추계학술대회논문집 Vol.2010 No.-

황화리튬 및 황화물계 고체전해질 소재 합성 연구

강다해,김경수 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.0

코로나 19 기간 중 여교사가 지각하는 일-가정 양립갈등과 직업평판이 소명의식에 미치는 영향

강다인(DA IN KANG),이지연(JI YEON LEE) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.22

목적 본 연구에서는 코로나 19기간 동안 유자녀 여교사들을 대상으로 일-가정 양립갈등, 직업평판, 소명의식, 괜찮은 일의 관계를 확인하여 구조방정식 모형과 일-가정 양립갈등, 직업평판과 소명의식의 관계에서 괜찮은 일의 매개효과를 검증하고자 하였다. 방법 이를 위하여 온라인으로 전국의 유자녀 여교사 286명을 대상으로 일-가정 양립갈등, 직업평판, 소명의식, 괜찮은 일을 측정하는 척도로 구성된 설문을 실시하였고, 응답자료 중 최종 249개를 분석에 사용하였다. 수집된 자료는 SPSS20.0과 AMOS26.0을 사용하여 구조방정식모형을 통해 분석하였다. 결과 수집된 자료를 분석한 주요 결과는 다음과 같다. 첫째. 본 연구의 주요 변인들 간의 상관분석을 실시한 결과 일-가정 양립갈등과 직업평판은 유의미한 부적 상관관계가 있는 것으로 나타났다. 둘째, 일-가정 양립 갈등과 괜찮은 일은 상관관계분석 실시 결과 정적 상관관계가 있는 것으로 나타났는데 최종 모형에서도 일-가정 양립갈등은 괜찮은 일을 정적으로 예측한다는 결과가 지지되었다. 셋째, 코로나19기간 동안 일-가정 양립 갈등과 여교사가 인식하는 직업평판이 소명의식에 이르는 경로에서 괜찮은 일의 매개효과에 대한 유의성을 검증한 결과, 괜찮은 일이 일-가정 양립갈등과 직업평판을 완전 매개하는 것으로 나타났다. 이러한 연구결과에 비추어 보면 일-가정 양립갈등과 직업평판이 각각 괜찮은 일을 매개로 소명의식에, 소명의식에 영향을 주는 것으로 나타났으며, 일-가정 양립갈등/직업평판과 소명의식의 관계에서 괜찮은 일은 완전매개효과의 영향을 주고 있음을 의미한다고 볼 수 있다. 결론 코로나19 기간 동안 여교사가 자신이 경험하는 일-가정 양립갈등과 직업평판이 소명의식으로 향하는 경로에서 괜찮은 일의 매개효과가 나타나는지를 살펴봄으로서 소명의식을 수행하는 선행기제를 파악하고 상담 개입방법의 기초적인 자료를 제공하고자 하였다. Objectives In this study, the relationship between work-family balance conflict, job reputation, sense of calling, and decent work was checked for female teachers with children during the COVID-19 period, and the relationship between the structural equation model and work-family coexistence conflict, job reputation and sense of calling was positive. The purpose of this study was to verify the mediating effect of work. Methods For this purpose, an online questionnaire consisting of scales measuring work-family balance conflict, job reputation, sense of calling, and decent work was conducted for 286 female teachers with children across the country, and the final 249 responses were used for analysis. The collected data were analyzed through the structural equation model using SPSS20.0 and AMOS26.0. Results The main results of analyzing the collected data are as follows. first. As a result of a correlation analysis between the major variables in this study, it was found that there was a significant negative correlation between work-family balance conflict and job reputation. Second, the results of correlation analysis showed that there is a positive correlation between work-family balance conflict and decent work, and the final model also supported the result that work-family balance conflict positively predicts decent work. Third, as a result of examining the significance of the mediating effect of decent work on the path from work-family balance conflict and female teachers' perceived job reputation to a sense of vocation during the COVID-19 period, decent work is associated with work-family balance conflict and job reputation. appeared to be completely mediated. In the light of these research results, it was found that work-family balance conflict and job reputation influence the sense of calling and calling through good work, respectively, and in the relationship between work-family balance conflict/professional reputation and the sense of calling It can be said that good work is influencing the perfectly mediating effect. Conclusions By examining whether the mediating effect of a decent job appears on the path that female teachers experience during the COVID-19 period, the work-family reconciliation conflict and professional reputation on the path toward the sense of vocation, it is necessary to identify the preceding mechanism for carrying out the vocation and the basis of the counseling intervention method. It was intended to provide relevant data.

색온도 가변 LED 조명 최적화 설계 및 제작

강다일,김근율,유영문,최희락,Kang, Da-Il,Kim, Kun-Yul,Yu, Young-Moon,Choi, Hee-Lack 한국광학회 2014 한국광학회지 Vol.25 No.2

본 논문에서는 현재 양산되고 있는 Red(621 nm), Green(530 nm), Blue(453 nm), Amber(590 nm) 등 4파장의 LED 조합을 이용하여 색온도가 가변되는 동안에도 평균연색지수 및 특별연색지수 R9 값이 높게 유지될 수 있도록 조명의 스펙트럼을 최적화하였다. 최적화 시 설계의 정확도를 높이기 위하여 실제 측정한 LED 스펙트럼을 사용하였고 광선과 기구물 사이의 상호 작용을 고려한 광선 추적 기법을 활용하여 수행하였다 또한 최적화 결과를 검증하기 위하여 색온도 가변 조명 장치를 직접 제작하였으며 광 특성을 평가한 결과 색온도가 3000 K에서 6000 K까지 변하는 동안 CRI 및 R9 값이 각각 87~90, 34~93을 나타내었다. In this paper the spectra of correlated color temperature (CCT) tunable white light-emitting diode (LED) luminaires, consisting of commercial red, green, blue, and amber LED chips, were optimized to increase color rendering index (CRI), and a special CRI of R9 for deep red color was obtained. To improve the design's accuracy, measured LED spectra were used instead of mathematically modeled ones. Real CCT tunable LED luminaires with CRIs of 87-90 and R9s of 34-93 were fabricated and demonstrated at CCTs of 3000-6000 K.