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RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
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윤화중,진성일,윤민중,윤혜수,이영하,최철규,이천배,신대현 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.2
Chungnam National University (CNU) is located in Daeduk Science Town, a center of science and technology in Korea, and it is expected to play an important role in the development of science in Korea. With this in mind, the basic natural sciences at CNU should be more actively supported in both education and research. Accordingly analysis and comparison of the basic science program at major college in Korea as well as those in the USA and Europe has been performed. The current cooperative relationship between CNU and research institutes in the science town has also been analyzed. This study has resulted in propositions to make more efficient the upbringing of the basic sciences at CNU, by focusing on the following points ; a) to improve academic affairs, b) to make graduate studies substantial, c) to secure highly qualified faculty members, d) to increase cooperation in research between CNU and reserch institutes, e) to enlarge research laboratories and acquire more equipment and facilities.
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윤현식,이두진,이영대 漢陽大學校 環境科學硏究所 2003 環境科學論文集 Vol.24 No.-
합류식 하수관거 월류수(Combined sewer overflow, CSOs)는 도시 인근 방류선 수체의 주요한 오염원의 하나로 알려져 있으며, 특히 강우초기 월류수의 경우 높은 오염부하가 일시에 방류되어 심각한 문제를 야기시킨다. 본 연구에서는 도시지역 합류식 하수관거 월류수의 발생 및 오염부하 특성을 분석하고, 초기강우에 의한 세척효과, 오염원 기여도 평가 등을 수행하였다. 강우사상에 따라 다소 차이가 있었으나, 합류식 하수관거 월류수가 건기하수에 비하여 오염부하가 전반적으로 높았으며, 특히 월류초기에는 매우 고농도로 측정되었다. 근사적인 오염물질 수지식을 통하여 관거퇴적물의 재부유에 의한 오염기여도가 가장 높았다. 무차원 L(V)곡선이 초기세척현상(First flush)을 잘 표현하였으며 SS, CODcr, T-P, T-N의 순으로 초기세척효과가 크게 나타났다. Combined sewer overflows(CSOs) have been considered as a major cause of water quality deterioration of natural water-courses in the vicinity of the heavily urbanized areas. The factors defining the magnitude and occurrence of CSOs are site specific. It is important to know exact properties of pollutants contained in CSOs to address water quality impacts that are caused by CSOs inputs to the receiving waters. This should be accompanied with an integrated analysis, evaluation programs, thus allowing us to an appropriate technology to alleviate the impacts. Pollutant concentrations measured during the storm events were generally higher than those of dry weather conditions except for T-N parameter and observed significant higher concentrations at the initial phase of CSOs. Based on the rough estimation by the proposed pollutant mass balance, it was found that a main part of wet weather pollutional loads originates from the resuspension of sewer bottom sediments. The dimensionless L(V) curves for determining whether first flush phenomenon occur or not were well described the pollutant flushing. These results show that a given fraction of volume contains a load of SS higher than CODcr, T-P, T-N in the initial phase of wet weather runoff.
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저널 베어링으로 지지되는 회전축계의 동특성에 미치는 감쇠영향
윤대현,윤한익 동의대학교 산업기술개발연구소 2001 産業技術硏究誌 Vol.15 No.-
In order to analyze the vibration characteristics of a rotor-bearing system, the coefficients of dynamic characteristics of this system are studied in consideration of the design parameter of journal bearing. The coefficients of dynamic characteristics of a journal bearing are calculated for 5 DOF including the coefficient of mometum. For the natural frequency of a rotor-bearing system the damping effect was analyzed by transfer matrix method. The stiffness of a journal bearing is increased as the rotating speed of shaft increased. And the stiffness of a journal bearing has a tendency to decrease as the clearance increased. The natural frequency of a rotor-bearing system supported by a journal bearing is generally increased as the damping coefficients increased.
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윤대식,한성현 경남대학교 신소재연구소 1996 論文集 Vol.6 No.-
가별 구조 제어 이론을(variable structure control)이론을 근간으로 하는 슬아이딩모드 제어의 원리는 오차상태공간내에 설정한 초평면의 양측에서 제어구조를 변환하여 슬라이딩모드를 발생시킴으로서 제어 대상을 초평면 구조를 변환하여 슬라이딩모드를 발생시킴으로서 제어 대상을 초평면(hypersurface)에 구속하는 것이다. 따라서 파라미터의 변동, 비선형성, 잡음 등에 둔감한 특성를 지닌 견실한 제어계를 실현할 수 있데 된다. 그러나 실제 산업용 로봇에 슬아이딩모드 제어를 적용시킬 경우, 오차상태 공간의 절환면에 도달할 때까지의 시간이 요구될 뿐만 아니라 제어 입력에 비례하여 채터링이 증가하는 단점을 지닌다. 특히, 매니퓰레이터의 자유도가 높아질수록 이러한 채터링과 연산지간지연 등이 가중되고 이러한 현상이 고속·고정도의 제어를 어렵게 하는 슬라이딩모드 제어의 단점으로 지적되고 있다. 한편 적응제어 방식은 플랜트의 동특성에 대한 제한된 정보만을 가지고 예측할 수 있는 불확실성에 대처할 수 있는 진보된 제어 방식이라 할 수 잇다. 본 연구에서는 기존의 슬아이딩모드 제어구조의 특성인 입력토크의 증가에 대한 채터링의 발생 현상을 보완하고 외부외란에 대해 견실한 제어 특성을 직접 적응제어 방식의 기준 모델 적응제어칙에 결합시킴으로서 실제 생산 공정에서 빈번히 수반되는 부하의 변동 및 외란의 증가 현상에 대해서도 정확한 위치 및 속도의 궤적 추종이 가능한 견실한 적응 슬라이딩모드 제어기를 제안한다. In this paper. it is proposed the adaptive-sliding mode control technique which is new approach to implement the robust control of industrial robot manipulator with external disturbances and parameter uncertainties. Over the past decade, the design of advanced control systems for industrial robotic manipulators has been a very active area of research and two major design categories have emerged. However, robot manipulators utilize independent joint controllers which control the joint angles separately through simple position servo loops. This basic control system enables .the manipulator to perform simple positioning tasks such a s pick-and-place operations. Therefore, it is severely limited in terms of precise tracking of fast trajectories and sustaining desirable dynamic performance for different payloads. Sliding mode control is a well-known technique for robust control of uncertain nonlinear systems. The robustness of sliding mode controllers can be shown in continuous time, but digital implementation may not preserve robustness properties because the sampling process limits the existence of a true sliding mode. The sampling process often forces the trajectory to oscillate in the neighborhood of the sliding surface. Adaptive control technique is particularly well-suited to robot manipulators where dynamic model is highly complex and may contain unknown parameters. Adaptive control algorithm is designed by using the principle of the model reference adaptive control method based upon the hyperstability theory. The contribution of this method is that the parameters of the sliding surface are replaced by time varying parameters whose value are calculated by an adaptation algorithm, which forces the errors to follow the behavior of a reference error model. The proposed control scheme has a simple structure. is computationally fast, and does not require knowledge of the complex dynamic model or the parameter values of the manipulator or the payload. Simulation results show that the proposed control method not only improves the performance of the system but also reduces the chattering problem of sliding mode control. Consequently, it is expected that the new adaptive sliding mode control algorithm will be suited for various practical applications of industrial robot control system.
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워터스, 파노라마 방사선사진과 Scanora 방사선사진의 상악동 점막비후 진단 결과의 비교
윤숙자,강병철,정현대 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.2
The purpose of this study was to compare the diagnostic performance of Waters' and panoramic view ; maxillary sinus posteroanterior and lateral scanography of ?? for mucosal thickening of maxillary sinus as well as to identify the utility of ?? for the detection of maxillary sinus disease. The assessment was done at 66 maxillary sinuses in 45 patients and the results were as follows ; 1. Estimation of presence or absence of mucosal thickening. The sensitivity, specificity, and positive and negative predictive value of maxillary sinus posteroanterior and lateral scanography were 0.865, 0.860, 0.921, and 0.805 respectively and slightly higher than those of Waters' and panoramic views, which were 0.832, 0.835, 0.903, and 0.728 respectively. However, paired t-test showed no significant differences in the diagnostic performance of the two pairs of imaging modalities. 2. Estimation of the types of mucosal thickening. The diagnostic accuracy for type Ⅰ, Ⅱ, Ⅲ was 75.3% on Waters' and panoramic view ; 77.9% on maxillary sinus posteroanterior and lateral scanography. It was higher on the latter, but showed no significant differences from that on the former. 3. Reliability of interpretation. In intraobserver and interobserver agreement, both overall rates of agreement and kappa-value were slightly higher on maxillary sinus posteroanterior and lateral scanography than on Waters' and panoramic views. There was no significant differences between the two pairs of imaging modalities. These results suggested that scanogram is a useful diagnostic radiography as well as Waters' and panoramic views for detection of maxillary sinusitis.
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