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Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
종 분포 모형을 이용한 구상나무림의 지속 및 쇠퇴에 관한 연구 - 전라남도 광양시 백운산을 중심으로 -
조선희 ( Seon Hee Cho ),박종영 ( Jong Young Park ),박정호 ( Jeong Ho Park ),이양근 ( Yang Geun Lee ),문이만 ( Lee Man Mun ),강상호 ( Sang Ho Kang ),김광현 ( Gwang Hyun Kim ),윤종국 ( Jong Guk Yun ) 한국산림과학회 2015 한국산림과학회지 Vol.104 No.3
The present study investigated the habitats of Korean fir trees (Abies koreana E. H. Wilson) on Mt. Baekwun (Baekwun-san), determined the current distribution, quantified the contribution of biological and nonbiological environmental factors affecting the distribution, derived actual and potential habitats, presented a plan for the establishment of protected areas, applied RCP 8.5 climate change scenario to analyze the effects of climate change on the future distribution of Korean fir trees, and predicted future potential habitats. According to the results of the study, 3,325 Korean fir trees (DBH >= 2.5 cm) inhabited Mt. Baekwun, and their distribution area was approximately 150 ha. Populations of Korean fir trees were confirmed to exist at an altitude of 900 m above sea level and were distributed up to 1,200 m. Based on potential distribution, areas appropriate for habitation by Korean fir trees were analyzed to be 450 ha, three times the current distribution area, with a focus on Sang Peak (Sang-bong), Eokbul Peak (Eokbul-bong), Ddari Peak (Ddari-bong), and Dosol Peak (Dosol-bong). The forest stands near Sang Peak, the main peak, were evaluated as those with the most appropriate potential for the habitation of Korean fir trees, and populations of the trees tended to prefer the northern slope rather than the southern slope. When climate change scenario RCP 8.5 was applied and future potential distribution was analyzed, the habitats were expected to decrease in area to 20 ha by 2050, with a focus on Sang Peak, and areas appropriate for habitation were predicted not to exist by 2080. Judging from such results, as global warming accelerates, the habitats of Korean fir trees are clearly expected to move from lowlands to highlands.
Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Sung Soo Kang,Hyun Ah Cho,김진석 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.1
Increase of NF-κB inducing kinase (NIK) is known to promote the proliferation of the hepatitis B virus-derived hepatocellular carcinoma (HCC) cells. Previously, we have reported that NIK-specific siRNA in cationic liposomes was shown to suppress the expression of NIK and the proliferation of HCC cells (Cho et al., 2009). More improved suppression of NIK, followed by the improved antiproliferative effect on Hep3B cells, was achieved when 5-FU was cotreated with siRNA. Furthermore, biodistribution study after intravenous injection of siRNA into Hep3B-bearing Balb/c nude mice revealed that siRNA was highly accumulated in liver, followed by tumor, lung, spleen, kidney and heart. When encapsulated in cationic liposomes, larger amount of siRNA was found in tumor owing to the protection of siRNA from enzymatic degradation and enhanced permeability by liposome, suggesting a possible therapeutic modality of siRNA in liver-targeting cationic liposomal formulation for the treatment of hepatitis B virus-derived HCC.
Effects of Red Ginseng Intake on Muscle Injury Due to Eccentric Exercise
Hyun Lyung Jung,Bong-Seok Kim,Chong-Do Lee,Hyun-Jong Na,Sung-Su Kim,Young-Chan Kim,Ho-Youl Kang 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.2
This study examined the effects of Korean red ginseng (RG) on muscle injury induced by eccentric exercise. Eighteen college male students were randomly assigned to either the RG group or the placebo group (P). The RG group ingested RG extract at 20 g/day (mixed with 200 mL of water) for 7 days prior to performing a downhill treadmill exercise and for 3 days after performing the downhill treadmill exercise, while the P group ingested 200 mL of water containing Agastachis Herba for 7 days prior to and 3 days following a downhill treadmill exercise. All subjects performed 2 bouts of a downhill treadmill exercise (6 ㎞, -14 degree slope, 12 ㎞/hr speed) with a 5 minute resting interval between bouts. Blood samples were drawn immediately before and after exercise, and at 1 hr, 24 hr, 48 hr, and 72 hr. Two-way repeated ANOVA documented that creatine kinase (CK) levels in the RG group were significantly reduced at 48 hr after exercise, as compared with P group, and remained constant until 72 hr after exercise. Lactate levels in the RG group were also significantly lower at 24 hr and 72 hr after exercise as compared to the P group. We conclude that supplementation of RGreduces CK levels and may prevent muscle injury induced by eccentric exercise.
Sung, Ho Kyung,Ma, Seung Hyun,Choi, Ji-Yeob,Hwang, Yunji,Ahn, Choonghyun,Kim, Byoung-Gie,Kim, Yong-Man,Kim, Jae Weon,Kang, Sokbom,Kim, Jaehoon,Kim, Tae Jin,Yoo, Keun-Young,Kang, Daehee,Park, Suekyung The Korean Society for Preventive Medicine 2016 예방의학회지 Vol.49 No.6
Objectives: We conducted a systematic review and meta-analysis to summarize current evidence regarding the association of parity and duration of breastfeeding with the risk of epithelial ovarian cancer (EOC). Methods: A systematic search of relevant studies published by December 31, 2015 was performed in PubMed and EMBASE. A random-effect model was used to obtain the summary relative risks (RRs) and 95% confidence intervals (CIs). Results: Thirty-two studies had parity categories of 1, 2, and ${\geq}3$. The summary RRs for EOC were 0.72 (95% CI, 0.65 to 0.79), 0.57 (95% CI, 0.49 to 0.65), and 0.46 (95% CI, 0.41 to 0.52), respectively. Small to moderate heterogeneity was observed for one birth (p<0.01; Q = 59.46; $I^2=47.9%$). Fifteen studies had breastfeeding categories of <6 months, 6-12 months, and >13 months. The summary RRs were 0.79 (95% CI, 0.72 to 0.87), 0.72 (95% CI, 0.64 to 0.81), and 0.67 (95% CI, 0.56 to 0.79), respectively. Only small heterogeneity was observed for <6 months of breastfeeding (p = 0.17; Q = 18.79, $I^2=25.5%$). Compared to nulliparous women with no history of breastfeeding, the joint effects of two births and <6 months of breastfeeding resulted in a 0.5-fold reduced risk for EOC. Conclusions: The first birth and breastfeeding for <6 months were associated with significant reductions in EOC risk.