Effect of a microbial phytase on growth performance, plasma parameters and apparent ileal amino acid digestibility in Youxian Sheldrake fed a low-phosphorus corn-soybean diet

He, Shaoping,Medrano, R.F.,Yu, Qifang,Cai, Yixin,Dai, Qiuzhong,He, Jianhua Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

Objective: This study investigated the effect of microbial phytase supplementation on growth performance, tibia ash, plasma parameters, apparent ileal digestibility (AID) of amino acid (AA) and apparent digestibility of nutrients in Youxian Sheldrakes fed with low-phosphorus (P) cornsoybean diets. Methods: A total of 350 Youxian Sheldrakes (7d old) were randomly divided into 5 treatment groups: positive control (PC) group has adequate available P diet (0.42% and 0.38%, starter and grower), negative control (NC) group were deficient in available P (0.32% and 0.28%, starter and grower) and NC diet was supplemented with 3 levels of microbial phytase (500, 750, and 1,000 U/kg). Results: Dietary supplementation of phytase in NC diet improved the average daily gain, increased the levels of serum calcium (Ca), tibia Ca and P, AID of AA and apparent digestibility of energy and Ca in starter stage (p<0.05). There was an increased (p<0.001) in the utilization of P from 17.3% to 23.9%. Phytase supplementation (1,000 U/kg) has shown that the AID of His, Thr, Val, indispensable AA, Glu, Pro, and dispensable AA was higher (p<0.05) than that of NC. Moreover, phytase supplementation improved (p<0.05) serum and tibia Ca and P, AID of AA and apparent digestibility of dry matter, crude protein, energy, P and Ca, and reduced (p<0.05) feed to gain ratio (F/G) and the levels of serum alkaline phosphatase in grower stage. Likewise, an increase (p<0.001) in the utilization of P was noticed from 12.6% to 17.2%. Supplement phytase at 750 U/kg improved the AID of His, Thr, Asp, Cys, Pro, and Ser (p<0.05). Conclusion: The microbial phytase supplement could improve growth performance, AID of some AA and apparent utilization of other nutrients in Youxian Sheldrakes, and reduce excreta P load to environment.

Differential Protein Expressions in Virus-Infected and Uninfected Trichomonas vaginalis

Ding He,Gong Pengtao,Yang Ju,Li Jianhua,Li He,Zhang Guocai,Zhang Xichen 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.2

3-D Seismic Images of Crust and Upper Mantle beneath Bohai Basin and its adjacent regions, Eastern China

Liu, Jianhua,Liu, Futain,He, Jiankun,Chen, Hui Korean Society of Earth and Exploration Geophysici 2000 지구물리 Vol.3 No.4

본 연구는 동지나 분지에서의 지각변형, 3차원적 지진파 속도 분포 및 화산암 원소의 지화학적 추적을 통하여, 분지의 지체구조적 및 대륙의 지체동역학적인 해석을 목적으로 수행되었다 This research was carried out in order to give some reasonable solutions on basin tectonics and on continental geodynamics, which are approached by using integrative researches on crustal deformation, 3-D seismic velocity reconstruction and geochemical tracing of volcanic rocks in the eastern China basin system.

Bandwidth Management of WiMAX Systems and Performance Modeling

( Yue Li ),( Jianhua He ),( Weixi Xing ) 한국인터넷정보학회 2008 KSII Transactions on Internet and Information Syst Vol.2 No.2

WiMAX has been introduced as a competitive alternative for metropolitan broadband wireless access technologies. It is connection oriented and it can provide very high data rates, large service coverage, and flexible quality of services (QoS). Due to the large number of connections and flexible QoS supported by WiMAX, the uplink access in WiMAX networks is very challenging since the medium access control (MAC) protocol must efficiently manage the bandwidth and related channel allocations. In this paper, we propose and investigate a cost-effective WiMAX bandwidth management scheme, named the WiMAX partial sharing scheme (WPSS), in order to provide good QoS while achieving better bandwidth utilization and network throughput. The proposed bandwidth management scheme is compared with a simple but inefficient scheme, named the WiMAX complete sharing scheme (WCPS). A maximum entropy (ME) based analytical model (MEAM) is proposed for the performance evaluation of the two bandwidth management schemes. The reason for using MEAM for the performance evaluation is that MEAM can efficiently model a large-scale system in which the number of stations or connections is generally very high, while the traditional simulation and analytical (e.g., Markov models) approaches cannot perform well due to the high computation complexity. We model the bandwidth management scheme as a queuing network model (QNM) that consists of interacting multiclass queues for different service classes. Closed form expressions for the state and blocking probability distributions are derived for those schemes. Simulation results verify the MEAM numerical results and show that WPSS can significantly improve the network`s performance compared to WCPS.

Insertional mutations exhibiting high cell-culture density HCD phenotypes are enriched through continuous subcultures in Chlamydomonas reinhardtii

Thung, Leena,He, Jing,Zhu, Qingling,Xu, Zhenyu,Liu, Jianhua,Chow, Yvonne The Korean Society of Phycology 2018 ALGAE Vol.33 No.1

Low efficiency in microalgal biomass production was largely attributed to the low density of algal cell cultures. Though mutations that reduced the level of chlorophyll or pigment content increased efficiency of photon usage and thus the cell-culture density under high-illumination growth conditions (e.g., >$500{\mu}mol\;photon\;m^{-2}\;s^{-1}$), it was unclear whether algae could increase cell-culture density under low-illumination conditions (e.g., ${\sim}50{\mu}mol\;photon\;m^{-2}\;s^{-1}$). To address this question, we performed forward genetic screening in Chlamydomonas reinhardtii. A pool of >1,000 insertional mutants was constructed and subjected to continuous subcultures in shaking flasks under low-illumination conditions. Complexity of restriction fragment length polymorphism (RFLP) pattern in cultures indicated the degree of heterogeneity of mutant populations. We showed that the levels of RFLP complexity decreased when cycles of subculture increased, suggesting that cultures were gradually populated by high cell-culture density (HCD) strains. Analysis of the 3 isolated HCD mutants after 30 cycles of subcultures confirmed that their maximal biomass production was 50-100% higher than that of wild type under low-illumination. Furthermore, levels of chlorophyll content in HCD mutant strains were similar to that of wild type. Inverse polymerase chain reaction analysis identified the locus of insertion in two of three HCD strains. Molecular and transcriptomic analyses suggested that two HCD mutants were a result of the gain-of-function phenotype, both linking to the abnormality of mitochondrial functions. Taken together, our results demonstrate that HCD strains can be obtained through continuous subcultures under low illumination conditions.

Novel NO removal using combined sodium erythorbate and FeIIEDTA system

Lirong Zhong,Feiqiang He,Beibei Dong,Jianhua Ding 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.10

To solve the difficulty of easy oxidation of FeIIEDTA in nitric oxide removal, FeIIEDTA combined with sodium erythorbate (SE) was employed for nitric oxide absorption. SE quickly reduced FeIIIEDTA to FeIIEDTA and maintained FeIIEDTA activity for a long time in the air. The influences of various operating parameters such as FeIIEDTA concentration, SE concentration, original pH value and temperature on NO removal in the bubbling device were examined preliminarily. The results reveal that the SE significantly enhanced nitric oxide absorption with FeIIEDTA. Nitric oxide absorption efficiency increased with the increase in the concentration of absorbent components or the decrease in temperature. Weak alkalinity (about pH 8.0) is beneficial to NO absorption. Besides, the NO removal efficiency continually decreased as the NO or O2 concentration increased, while the NO removal efficiency increased first and then decreased as the SO2 concentration increased. However, pH and temperature hardly affected nitric oxide absorption efficiency at FeIIEDTA with high concentration. Finally, the kinetic studies demonstrated that NO absorption by mixed FeIIEDTA and SE was more effective in reducing total mass transfer resistance and enlarging the NO flux compared with the normal FeIIEDTA approach. The absorption process was controlled by the liquid film.

A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis

Qiuyue Wang,Lifeng Chen,Jianhua Li,Jun Zheng,Ning Cai,Pengtao Gong,Shuhong Li,He Li,Xichen Zhang 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.3

Insertional mutations exhibiting high cell-culture density HCD phenotypes are enriched through continuous subcultures in Chlamydomonas reinhardtii

Leena Thung,Jing He,Qingling Zhu,Zhenyu Xu,Jianhua Liu,Yvonne Chow 한국조류학회I 2018 ALGAE Vol.33 No.1

Low efficiency in microalgal biomass production was largely attributed to the low density of algal cell cultures. Though mutations that reduced the level of chlorophyll or pigment content increased efficiency of photon usage and thus the cell-culture density under high-illumination growth conditions (e.g., >500 µmol photon m-2 s-1), it was unclear whether algae could increase cell-culture density under low-illumination conditions (e.g., ~50 µmol photon m-2 s-1). To address this question, we performed forward genetic screening in Chlamydomonas reinhardtii. A pool of >1,000 insertional mutants was constructed and subjected to continuous subcultures in shaking flasks under low-illumination conditions. Complexity of restriction fragment length polymorphism (RFLP) pattern in cultures indicated the degree of heterogeneity of mutant populations. We showed that the levels of RFLP complexity decreased when cycles of subculture increased, suggesting that cultures were gradually populated by high cell-culture density (HCD) strains. Analysis of the 3 isolated HCD mutants after 30 cycles of subcultures confirmed that their maximal biomass production was 50-100% higher than that of wild type under low-illumination. Furthermore, levels of chlorophyll content in HCD mutant strains were similar to that of wild type. Inverse polymerase chain reaction analysis identified the locus of insertion in two of three HCD strains. Molecular and transcriptomic analyses suggested that two HCD mutants were a result of the gain-of-function phenotype, both linking to the abnormality of mitochondrial functions. Taken together, our results demonstrate that HCD strains can be obtained through continuous subcultures under low illumination conditions.

The Throughput Order of Multicast Traffics with Physical-Layer Network Coding in Random Wireless Ad Hoc Networks

Chen, Chen,Bai, Lin,He, Jianhua,Xiang, Haige,Choi, Jin-Ho The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.3

This paper attempts to address the effectiveness of physical-layer network coding (PNC) on the throughput improvement for multi-hop multicast in random wireless ad hoc networks (WAHNs). We prove that the per session throughput order with PNC is tightly bounded as ${\Theta}((n\sqrt{m}R(n))^{-1})$ if $m=(R^{-2}(n))$, where n is the total number of nodes, R(n) is the communication range, and m is the number of destinations for each multicast session. We also show that per-session throughput order with PNC is tight bounded as ${\Theta}(n^{-1})$, when $m={\Omega}(R^{-2}(n))$. The results of this paper imply that PNC cannot improve the throughput order of multicast in random WAHNs, which is different from the intuition that PNC may improve the throughput order as it allows simultaneous signal access and combination.

A kind of core cross-linked ‘‘semi-amphiphilic” polymer assemblies for controlled release of corrosion inhibitors and autonomous corrosion alarm

Ying Cao,Aoqi Zhu,Jianmin He,Jianhua Wu,Mingjin Xue,Yiting Xu,Birong Zeng,Guorong Chen,Lizong Dai 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-

A novel kind of core cross-linked assemblies formed from a ‘‘semi-amphiphilic” block copolymer, poly(syringaldehyde methacrylate)-block-poly (dopamine methacrylate-co-methyl methacrylate-costyrene)(PSMA-b-P(DMA-co-MMA-co-St)), was designed and served as the carrier for anti-corrosiveand corrosion detective molecules. Different from the core cross-linked micelles formed by the amphiphilicpolymers in water, the core cross-linked assemblies in this research were formed from the ‘‘semiamphiphilic”block copolymer, which took advantage of the different compatibility between epoxy andthe blocks in the polymer, leading to the self-assembly behavior happening in the organic resin, whilethey shared the advantages of traditional core cross-linked micelles with the properties of being stable,stimuli-responsive and multifunctional. The pH-responsive properties of the assemblies and their complexationwith Fe3+ caused by the corrosion of the steel were confirmed by UV spectrophotometry andXPS analysis of the corrosive products. The controlled release of corrosion inhibitors was fully reflectedby the expansion of the capacitor ring and the increase of the coating resistance (Rc) with the extensionof the immersion time, which was got via fitting the electrochemical impedance spectroscopy (EIS) datawith the equivalent electric circuits. Ultimately, the anti-corrosive mechanism of smart coating with thefunctional core cross-linked assemblies were also illustrated.