Clinical Significance of Classification of Graves` Disease According to the Characteristics of TSH receptor Antibodies

(Won Bae Kim),(Hyun Kyung Chung),(Young Joo Park),(Do Joon Park),(Hong Kyu Lee),(Bo Youn Cho) 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.3

N/A Background:It has been widely accepted that the epitope (s) and/or functional characteristics of thyrotropin receptor antibodies (TSHRAb) from Graves` patients are heterogenous among patients. However, the clinical significance of such heterogeneity has not been systematically evaluatedyet. We were to elucidate and find the clinical significance of heterogeneity for TSH receptor antibodies in Graves` disease. Methods:We measured stimulating TSHRAb (TSAb) activities using CHO-hTSHR cells, FRTL-5 cells and chimeric receptor expressing cells (Mc1+2 and Mc2), specific blocking TSHRAb (TSBAb) activities using Mc2 cells and TBII activities using porcine thyroid membrane in 136 patients with untreated hyperthyroid Graves` disease. Results: Based on various TSHRAb activities from each patient, the patients could be categorized into 7 subgroups by cluster analysis; 1) Group 1 (n=41) was characterized by moderate TSAb activities bothin CHO-hTSHR cells and in FRTL-5 cells, typical TSAb epitope, rare blocking antibodies and high TBII activities. 2) Group 2 (n=16) was characterized by the presence of blocking TSHRAb in most patients, albeit the other characteristics were the same as those in Group 1. 3) Group 3 (n=19) patients had low TSAb activities bothin CHO-hTSHR cells and in FRTL-5 cells, seldom had blocking TSHRAb, but they had high TBII activities. 4) Group 4 (n=30) could be categorized as `mild disease` group, as they had low activities in all kinds of TSHRAb assay and had low antim icrosomal antibody activities. 5) Group 5 (n=14) was characterized by moderate TSAb activities with atypical epitope (s), rare blocking TSHRAb and moderate TBII activities. 6) Group 6 (n=10) patients had veryhigh TSAb activities with typical epitopes, seldom blocking TSHRAb and low TBII activities. 7) Group 7 (n=6) was characterized by very high TSAb activities with atypical epitopes and high TBII activities. Pretreatment serum thyroid hormone level was low only in group 4 patients compared to the other 6 groups (p<0.05). The size of goiter was significantly larger in those in group 1 and group 3 (p<0.05) compared to the other 5 groups. The prevalence of clinically significant ophthalmopathy was higher in group 2 patients than the other 6 groups (50%vs.27.5%,p=0.06). Among 6 kinds of TSHRAb activities, only the blocking TSHRAb activity was significantly associated with the presence of ophthalmopathy in multivariate analysis. Conclusion:These results suggest that the differences inepitopes for TSAb or the presence of blocking TSHRAb is not a major factor in determining the degree of thyrotoxicosis in Graves` disease. Although the pathogenic mechanism is not clearyet, we suggest that patients with ophthalmopathy have different TSHRAb repertoire from those without ophthalmopathy in Graves` disease.

Cycloheximide가 Mouse肝臟의 인산염 分解酵素의 活性에 미치는 影響

趙賢熙,鄭鎬三,李圭植 漢陽大學校 環境科學硏究所 1985 環境科學論文集 Vol.6 No.-

Cycloheximide는 Streptomyces griseus에서 추출한 항진균성 항생제로 진균의 성장을 저해하는 물질로 알려져 왔으며 포유동물의 세포에서 ribonucleic acid와 단백질 합성을 저해한다고 생화학적 방법에 의하여 보고되었다. 최근에 이르러 cycloheximide의 투여로 간세포내 소기관인 조면내형질세망 및 Golgi complex의 손상에 의해서 단백질합성이 저해된다고 형태학적으로 연구 보고된 바 있다. 이와같은 cycloheximide의 투여로 간세포가 손상을 입어 단백질합성이 저해될 때 간세포내 인산염분해효소로 분포되어 있는 acid phosphatase와 adenosine triphosphatase 활성을 추구하였다. 실험동물로는 실험실에서 사육한 건강한 ICR계 체중 20∼30gm의 mouse를 사용하였으며 정상대조군과 cycloheximide 투여군으로 구분하고 실험군은 다시 cycloheximide 투여 후 6시간군, 12시간군, 24시산군 및 36시간군으로 나누었고 각군에 10마리씩 배정하였다. Cycloheximide는 mouse의 체중 ㎏당 4㎎ 되게 생리적 식염수에 용해하여 0.1㎖를 복강내에 투여후 6시간, 12시간, 24시간 및 36시간 후에 희생시켜 간장을 적출하고 10% neutral formalin에 고정한 다음 cryostat로 두께 15㎛ 되게 동결절편을 만들어 Gomori법으로 acid phosphatase활성과 Wachstein 및 Meisel법으로 adenosine triphosphatase 활성을 관찰한 결과 다음과 같은 결과를 얻었다. 1. Cycloheximide 투여후 6시간, 12시간군 및 24시간군의 mouse 간소엽에서 중심 정맥부로 부터acid phosphatase 활성이 증가되기 시작하여 36시간군에서는 간소엽 전역에 중등도 이상의 강한 활성이 관찰되었다. 2. Cycloheximide 투여후 24시간군에서 부터 간소엽의 중심 정맥부에 ATPase 활성이 감소되기시작하여 36시간 경과군에서는 간소엽 전역에 음성반응을 나타내었다. 상기와 같은 결과는 cycloheximide 투여로 간장의 acid phosphatase는 간장이 손상을 입을 때 그 활성이 보다 강한 양성반응을 보이고 adenosine triphosphatase는 역으로 활성이 약해진다고 사료된다. It has been knwon that cycloheximide, an antifungal antibiotics extracted from streptomyces griseus, inhibits the growth of fungus. It has been reported by several investigators that this drug inhibits ribouncleic acid activity and protein synthesis of the mammalian cell. Recently, several reporters indicated that the cycloheximide causes the damage of hepatocyte. The observations by electron microscopic, histochemical and histological studies revealed that cycloheximide inhibits the RNA and protein synthesis by the injury of rough endoplasmic reticulum and the desocciation of polysome in hepatocyte. This study attempted, on the activities of acid phosphatase adenosine triphosphatase, phosphatelysis-enzymes distributed in the hepatocytes in the mouse liver. Healthy albino mice(ICR strain) of 20gm±in body weight were used as experimental animals which were divided into the control and cycloheximide treated groups. The animals of the cycloheximide treated groups were administered cycloheximide. (dissolved in physiological saline sol., by intraperitoneal injection in a dose of) 4mg per kg of body weight in a volume of 0.1ml, intraperitoneally. The experimental animals were sacrificed at intervals of 6, 12, 24 and 36 hours after administration of cycloheximide, respectively. The liver specimens were fixed with 10% neutral formalin, the sections of 15㎛ thickness were stained by Gomori, Wachstein and Meisel`s method for the detection of acid phosphatase and adenosine triphosphatase activities. The results were obtained as follows; 1. Acid phosphatase activity was increased in the central zone of liver lobule at the 6 hour and 24 hour period of the cycloheximide treated group, moderately positive activity was showed in the liver lobule at the 36 hour period group. 2. Adenosine triphosphatase activity was decressed in the centrol zone of liver lobule at the 24 hour period of the cycloheximide treated group. Negative reactions were showed in the whole liver lobules at the 36 hour period group. Consequently, it is supposed that cycloheximide caused increase of liver acid phosphatase result from liver damage, while adenosine phosphtase activity of the liver was decreased.

공동주택관리의 효율성 증대 방안 : 진주시 중심으로

조현화,김규 진주산업대학교 2000 論文集 Vol.39 No.-

This study is attempted to provide information that will be useful to the private managers of collective houses who intend to improve the collective house management. The objective of the study was an analytical survey on the improvement of the collective house management that would make every occupants' lives more convenient and safe, and minimize the costs of housing management. For this purpose a total of occupants of apartments in Chinju were put to questionnaire. The several facts that would make housing management improved were obtained from the survey. The alternatives for the effective management of the collective house were showed as follows : First, the effective management of the collective house has mainly been dependent on the laws and institutions related to it, and how to manage it such as flaw repair, maintenance, safety management, and so on. Second, if housing management is to be effective related laws are systematically and rationally improved. Laws and regulations have taken a negative effects on housing management. The law requires that adequate measure be devised if government concerned implement regulations that can influence the housing management. The occupants may manage the collective house for themselves instead of managers being licensed by the government to conduct their management. The laws and regulations suitable for direct management in actual must be revised and acted. These changes have improved the their existing system, and come into effect on the housing management. Third, there is no doubt that managers' ways of thinking and values have great influence on the housing management. There should, therefore, be established a institution so that the mangers may learn how to manage the housing management in a more professional way. Forth, if housing management is to be effective a comprehensive, positive participation of occupants is necessary. Occupants should maintain a high level of interest in housing management. Meaningful cooperative behaviors, from which every occupants could benefit, should be planned and pursued as well. Occupants's behaviors beneficial for housing management in actual are expected to produce significant results.

LC, Acute : O-061 ; Mode of cirrhosis related complication in Korean patients with liver cirrhosis -A prospective multicenter cohort study

( Hyun Young Woo ),( Jeong Won Jang ),( Sang Gyune Kim ),( Chang Hyeong Lee ),( Tae Yeob Kim ),( Won Young Tak ),( Sung Kyu Choi ),( Mong Cho1 ),( Jong Young Choi ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Cirrhosis-related complications are indications for transplantation. However, the mode of complication has not been evaluated so far. To better address this issue, we investigated the mode of cirrhosis related complication and impact of these complications on survival in prospective, multicenter, inception cohort study of subjects with liver cirrhosis presenting with the first onset of decompensated complication. Methods: In this observational study, 1515 patients with the confirmed onset time of the first decompensated complication were enrolled. The mortality risk of cirrhosis-related complications, including ascites, portal hypertensive gastrointestinal bleeding (PHGB), spontaneous bacterial peritonitis (SBP), hepatic encephalopathy (HE), hepatorenal syndrome (HRS), was analyzed in these cirrhotic patients. Result: As the first complication, ascites was the most frequent (53.8%), followed PHGB (36.2%), HE (7.6%), SBP (1.7%) and HRS (0.7%). During follow-up (mean 32±47 months), 484 (31.9%) experienced subsequent decompensations and the proportion of subsequent complication was consistently changed; the proportion of HE, SBP, HRS increased as time progressed. The interval between subsequent decompensative events was progressively shortened. The mortality rate according to the first complication was as follows; Ascites =PHGB> HE=SBP>HRS. The mortality rate of patients experienceing subsequent complication was significantly higher than those without subsequent complication. According to the kinds of subsequent complication, survival of HE was also higher than HRS but lower than ascites or PHGB and similar to that of SBP and this difference of survival disappeared after repeated complication. Conclusion: The mortality risk correlated with initial complication and the risk increases as the number of complication episodes is repeated. Patient`s mean age was 56±11.50 years and 72.1% was male. The cause of liver cirrhosis is hepatitis B virus (38.6%), alcohol (35.5%) and hepatitis C virus (8.9%).

흉배혈관의 근피 천공분지에 기초한 유리피판의 임상적 이용

조규성,김대영,안호범,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The latissimus dorsi muscle or musculocutaneous flap is one of the most useful flaps in reconstructive surgery. This flap has many advantages, such as its reliable anatomy, long pedicle with large caliber vessels, minimal functional deficit of the donor site, and low incidence of donor site complications. However, the bulkiness of the flap has been considered a disadvantage, so various modifications of technique have been devised. The cutaneous portion of the flap can be safely elevated based on the cutaneous perforating branch of the thoracodorsal vessel. From March 1997 to February 1998, 10 patients underwent reconstructive procedures with thoracodorsal perforator-based free flaps. The composition of the flaps varied in accordance with the nature of the defect. The variances in the flaps were as follows; 3 were cutaneous, 6 musculocutaneous, and 1 osteomusculocutaneous including the rib. All flaps survived with good contour. We concluded that this thin and reliable flap was useful for reconstruction of various defects, and that the composition of the flap, such as subcutaneous, muscle and bone, gave it considerable flexibility as needed.

스포츠 센터 회원의 이용만족도에 관한 분석

조충현,연규한 圓光大學校 附設 體力科學硏究所 2002 體力科學硏究 Vol.25 No.1

This study is aimed at theoretically investigating the satisfaction of sports center members and, based on the findings, conducting in parallel a practical study to introduce a marketing concept in sports activities. For this purpose, a survey with questionnaires was made to the members of sports centers located in Jeonbuk provincial area. The findings of the survey are summarized as follows: First, from the analysis into satisfaction of sports center members using facilities by sex, male members showed statistically more significant response of satisfaction to lobby, sports program and health diagnosis program than females, while both seemed to be unsatisfied with admission fees. There was no significant difference in satisfaction between sexes regarding parking lot and hygiene. Second, from the analysis by age, the members responded satisfactorily to the contents and health diagnosis program with statistically significant difference. However, there was no significant difference in the satisfaction with locker room, lobby, parking lot and items of instruments. Third, from the analysis into satisfaction depending on incomes of members, there was statistically significant difference in lobby, hygiene and program contents. The members seemed to be satisfied with lobby and contents but not satisfied with hygiene.

남·여 레슬링 선수들의 비 시즌 동계훈련이 무산소성 파워 및 혈중 젖산과 암모니아농도에 미치는 영향

조현철,장호성,김종규 龍仁大學校 體育科學硏究所 2003 體育科學硏究論叢 Vol.13 No.2

The purpose of this study was to investigate the effect of an 8wk winter training program on body composition, anaerobic power and blood fatigue components. To examine this question, 6 males and 6 females amateur athletic wrestlers were randomly assigned to supervised an 8wk winter training program. Lactate, amonia concentration was measured before and wingate anaerobic test after 4wk to 8wk exercise program. Body composition was determined from electric impedance absorptiometry, wingate anaerobic test from a Lode wingate test to 30" . Results showed that body composition just changed in male, but muscle mass did not change in female. Male and female amateur athletic wrestlers increased anaerobic peak power and mean power all. Blood lactate and ammonia concentration decreased when measured 4wk into 8wk to the training program, but female amateur athletic wrestlers increased it. These results suggest that winter training program for amateur athletic wrestlers should be alter body composition, anaerobic power and blood fatigue components in winter training program.

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

상 요추 추간판 탈출증의 수술적 치료

조태현,송준혁,김명현,박향권,김성학,신규만,박동빈 梨花女子大學校 醫科大學 醫科學硏究所 2000 EMJ (Ewha medical journal) Vol.23 No.3

Objectives : Lumbar disc herniations at the L1-L2, L2-L3, and L3-L4 level are rare. The purpose of this study is to observe the variable clinical finding of upper lumbar disc herniations and to provide proper treatment modality. Patients and Methods : Between 1998 Jan. and 1999 Dec., seventeen patients with upper lumbar disc herniations who were undergone surgery in our institute were retrospectively evaluated. Patients were evaluated based on following factors : age, sex, aggravating factor, symptoms and signs type of disc herniation, type of surgery, and outcome of post-surgery. Results : The incidence of lumbar disc herniation at the level of L1-2, L2-3 and L3-4 is 6.1% with declining frequency as the level ascends. The peak age incidence is 6th decade in both sex. Preoperative symptomes and signs are variabe. In sixteen cases, posterior approach was done. In these cases, twelve cases were performed microscopic partial hemilaminectomy, and four cases with spinal stenosis were performed total laminectomy and posterior screw fixation with bone fusion. In one case, lateral extracavitary approach was done. In results of operation, fourteen cases showed more than good grade(82%). Conclusion : The age incidence of lumbar disc herniations at the level of L1-2, L2-3 and L3-4 older than lumbar disc herniations at the lower level of L4-5 and L5-S1. The signs and symptoms are variable. In our cases, most of the patients were performed posterior approach with microscopic partial hemilaminectomy except the patients combined spinal stenosis. One case was performed a lateral extracavitary approach because the risk of the cord and cauda equina injuries. The prognosis of upper lumbar disc herniations after treatment with only microscopic partial hemilaminectomy and diskectomy is comparable with the prognosis of lumbar disc herniations at the lower level.

Binaural beat 훈련이 골프선수들의 생리적 변화 및 골프 퍼팅수행력에 미치는 영향

조현철,김성연,임성규 용인대학교 국제스포츠과학연구원 체육과학연구소 2008 體育科學硏究論叢 Vol.18 No.1

The purpose of this study was to increase a efficiency on physiological reaction and putting ability of golfer when they were used Binaural-beat. Participants were fourteen man aged 21-27 years in university golfer. They were tested both before and after Binaural-beat by EEG, EMG, Heart Rate and putting of three times at 4m range. The results as follow: 1. Alpha wave was a statistical significantly increased, beta and theta wave were not statistical significantly increased when it apply the Binural-beat. 2. EMG on apply the Binural-beat was lower than meditation. Especially, there were statistical significantly difference in Trapezius and Erector spinae, were not statistical significantly difference in Tibialis anterior. 3. There was no statistical significantly difference in heart rate when it apply the Binural-beat. As this results, We Binural-beat training was given a positive influence in brain alpha wave and lower of muscles activity. Therefore, we think that it can help to concentration elevation.