Multi‑tissue lipotoxicity caused by high‑fat diet feeding is attenuated by the supplementation of Korean red ginseng in mice

Seon‑A Jang,Seung Namkoong,이성률,Jin Woo Lee,Yuna Park,Gyeongseop So,Sung Hyeok Kim,Mi‑Ja Kim,Ki‑Hyo Jang,Alberto P. Avolio,Sumudu V. S. Gangoda,Hyun Jung Koo,Myung Kyum Kim,Se Chan Kang,Eun‑Hwa Sohn 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Background Excessive intake of fat, one of the causes of obesity, is associated with low-grade infammation in various susceptible organs and eventually causes tissue toxicity. This study examines the multifaceted suppressive efects of Korean red ginseng extract (KRG) on high-fat diet (HFD)-induced lipotoxicity and infammatory responses in the aorta, liver, and brain. Methods Male C57BL/6 mice were fed HFD with or without KRG for 12 weeks. The improvement efect in KRG on lipotoxicity and infammatory potential was determined in the blood and the aorta, liver, and brain tissues. Results KRG signifcantly inhibited 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity by >20% in vitro. KRG supplementation suppressed HFD-associated body weight gain, lipid profle changes, and excessive fat deposition in the liver and increased leptin, insulin, and ALT levels in the blood. Infammatory markers in the aorta, liver, and brain were also signifcantly reduced by KRG treatment. In microvascular endothelial cells, the 15% cyclic stretch-mediated upregulation of ICAM-1 and vascular cell adhesion protein-1 (VCAM-1) expression was signifcantly attenuated in the presence of KRG. Conclusion KRG supplementation attenuates HFD-mediated body weight gain, lipid profle changes, and multi-tissue infammatory responses.

The likelihood of achieving pregnancy through timed coitus in young infertile women with decreased ovarian reserve

Koo, Hwa Seon,Song, In Ok,Cha, Sun Hwa,Park, Chan Woo,Kim, Hye Ok The Korean Society for Reproductive Medicine 2018 Clinical and Experimental Reproductive Medicine Vol.45 No.1

Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum $anti-M{\ddot{u}}llerian$ hormone (AMH) levels ( < 25th percentile). Methods: A total of 202 patients younger than 35 years old were recruited retrospectively between 2010 and 2012. Ninety-eight women had normal serum AMH levels (25-75th percentile), 75 women had low serum AMH levels (5th ${\leq}$ & < 25th percentile) and 29 women had very low serum AMH levels ( < 5th percentile), according to reference values for their age group. Results: The clinical pregnancy rate was positively associated with AMH levels, but this trend did not reach statistical significance (43.9% vs. 41.3% vs. 27.6% in the normal, low, and very low AMH groups, respectively). The time to pregnancy was longer in the very low AMH group than in the normal AMH group ($13.1{\pm}10.9months$ vs. $6.9{\pm}6.1months$, p= 0.030). The cumulative live birth rate over 18 months was lower in the very low AMH group than in the normal AMH group, with marginal significance (20.0% vs. 55.9%, p= 0.051). The duration of infertility was negatively correlated with achieving pregnancy (odds ratio, 0.953; 95% confidence interval, 0.914-0.994; p= 0.026). Conclusion: Conservative management, such as timed coitus with or without superovulation, should be considered in young patients who have low ovarian reserve without any infertility factors. However, for women with a long duration of infertility or very low serum AMH levels, active infertility treatment should be considered.

Laparoscopic management of early primary peritoneal pregnancy: a case report

Koo, Hwa-Seon,Bae, Ju-Youn,Kang, Inn-Soo,Koong, Mi-Kyoung,Kim, Hye-Ok,Cha, Sun-Hwa,Choi, Min-Hye,Kim, Ji-Young,Yang, Kwang-Moon The Korean Society for Reproductive Medicine 2011 Clinical and Experimental Reproductive Medicine Vol.38 No.2

Peritoneal pregnancy is an implantation in the peritoneal cavity exclusive of tubal, ovarian, or intra-ligamentary implantations. This is a rare obstetric complication with high maternal mortality and even higher perinatal mortality, and secondary type was most common. Risk factors for peritoneal pregnancy are previous history of extrauterine pregnancy or tubal surgery pelvic post-inflammatory status or presence of an intrauterine device. As it is a life-threatening condition, expectant management carries a risk of sudden life-threatening intra-abdominal bleeding and a generally poor fetal prognosis. So, when it is recognized, immediate termination of pregnancy is usually recommended. Early diagnosis of peritoneal pregnancy is difficult, but is important by their life threatening progress course to patients. Recently, we experienced primary peritoneal pregnancy which meets both the original and modified criteria. In this paper, we reported the case of early diagnosed and successfully treated peritoneal pregnancy despite of their diagnosis was incidentally.

학술상 : 학술상 (생식내분비학)

( Hwa Seon Koo ),( Hyun Jeong Yi ),( Min Young Lee ),( Min Hye Choi ),( Na Young Sung ),( Yu Im Hwang ),( In Ok Song ),( Mi Kyoung Koong ),( Inn Soo Kang ),( Kwang Moon Yang ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

Objective: To evaluate whether increased peripheral blood inflammatory immune cell can induce decrease of uterine blood flow in patients with unexplained recurrent spontaneous abortion (RSA). Methods: This study designed prospectively 33 pregnant women between 5 to 7 gestational weeks with a history of unexplained RSA included in this study. 47 normal pregnant women between 5 to 7 gestational weeks without history of infertility and/or RSA included as a control. Peripheral blood natural killer (pbNK) (CD3-/56+) fractions among peripheral blood monocyte (PBMC) were checked by flow cytometry. Uterine color-pulsed Doppler trans-vaginal ultrasound for evaluation of uterine radial artery RI was checked. Uterine radial artery resistance index (RI) compared between study and control group. After then, uterine radial artery RI was compared between high pbNK cell fraction above 12.1% among PBMC and normal pbNK cells fraction below 12.1%. Correlation between pbNK cell fraction to uterine radial artery RI was also evaluated. Results: Uterine radial artery RI in early pregnancy was significantly higher in patients with RSA than that of normal control (0.60 ± 0.14 vs. 0.54 ± 0.12, P = 0.039). Especially, the mean value of uterine radial artery RI in RSA patients with elevated pbNK cells was significantly increased than that of normal control (0.62 ± 0.13 vs. 0.54 ± 0.12, P =0.029). Otherwise, pbNK cell fractions among PBMC displayed strong positive correlation to uterine radial artery RI (Pearson`s correlation coefficient P=0.001, r = 0.667). Conclusion: Increased pbNK cells can evoke decreased uterine blood flow by their pro-inflammatory action on micro vascular structure such as uterine radial artery. This can be a one causative mechanism of inducing spontaneous abortion by increased NK cells. But, larger scaled study is needed for clarify our results.

Resistance of uterine radial artery blood flow is positively correlated with peripheral blood NK cell fraction in patients with unexplained recurrent spontaneous abortion

( Hwa Seon Koo ),( Hyun Kyung Ahn ),( Chan Woo Park ),( Ji Hee Yoo ),( Sun Hwa Cha ),( Inn Soo Kang ),( Kwang Moon Yang ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

A high response to controlled ovarian stimulation induces premature luteinization with a negative impact on pregnancy outcomes in a gonadotropin-releasing hormone antagonist cycle

Koo, Hwa Seon,Cha, Sun Hwa,Kim, Hye Ok,Song, In Ok,Min, Eung Gi,Yang, Kwang Moon,Park, Chan Woo The Korean Society for Reproductive Medicine 2015 Clinical and Experimental Reproductive Medicine Vol.42 No.4

Objective: The goal of this study was to investigate the relationship between serum progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration and the pregnancy rate among women undergoing controlled ovarian stimulation for in vitro fertilization (IVF) or intracytoplasmic sperm injection-embryo transfer (ICSI-ET) using a flexible antagonist protocol. Methods: This prospective study included 200 IVF and ICSI-ET cycles in which a flexible antagonist protocol was used. The patients were divided into five distinct groups according to their serum P4 levels at the time of hCG administration (0.80, 0.85, 0.90, 0.95, and 1.00 ng/mL). The clinical pregnancy rate (CPR) was calculated for each P4 interval. Statistically significant differences were observed at a serum P4 level of 0.9 ng/mL. These data suggest that a serum P4 concentration of 0.9 ng/mL may represent the optimal threshold level for defining premature luteinization (PL) based on the presence of a significant negative impact on the CPR. Results: The CPR for each round of ET was significantly lower in the PL group defined using this threshold (25.8% vs. 41.8%; p=0.019), and the number of oocytes retrieved was significantly higher than in the non-PL group ($17.3{\pm}7.2$ vs. $11.0{\pm}7.2$; p=0.001). Elevated serum P4 levels on the day of hCG administration were associated with a reduced CPR, despite the retrieval of many oocytes. Conclusion: Measuring serum P4 values at the time of hCG administration is necessary in order to determine the optimal strategy for embryo transfer.

The Correlation between Maternal Ante- and Postpartum Depression and Mode of Delivery: Preliminary Study

( Hwa Seon Koo ),( Young Ran Kim ),( Jin Young Park ),( Kyungun Jhung ),( Kyungmi Chung ),( Hee Young Cho ) 대한주산의학회 2020 Perinatology Vol.31 No.4

Objective: To investigate the relationship of prenatal and postnatal depression and mode of delivery. Methods: The study population consisted of 35 pregnant women who underwent antenatal care in Bundang CHA Medical Center from October 2018 to September 2019. Among them, 20 women underwent cesarean section (c-sec) and 15 women underwent vaginal delivery (VD). The survey was conducted at 34 to 36 gestational weeks and at postpartum 4 weeks consecutively using Korean version of Edinburgh Perinatal/Postnatal Depression Scale (EPDS). To evaluate the association between maternal ante- and postpartum depression and mode of delivery, we analyzed the differences of EPDS according to mode of delivery and pre- and postpartum. Results: The mean scores of EPDS before delivery was 7.2±5.0 in C-sec group and 5.8±4.7 in VD group, but it was not statistically significant (P=0.390). The EPDS scores at postpartum was significantly higher in women who underwent C-sec compared to VD group (9.2±4.9 vs. 7.6±5.4, P=0.010; respectively). Also when comparing the mode of delivery in fourteen women with EPDS 9 or above at postpartum, C-sec rate (64.3%) was significantly higher than VD rate (35.7%) (P=0.010). In both groups, the scores of EPDS were increased after delivery with statistical significance (P=0.039). Conclusion: C-sec delivery could increase the EPDS at postpartum and delivery itself could result in maternal depressive symptoms during postpartum period regardless of mode of delivery.

원인불명의 습관성 유산 환자에서 말초혈액 자연살해세포의 증가가 임신초기 자궁혈류의 변화에 미치는 영향

구화선 ( Hwa Seon Koo ),이현정 ( Hyun Jeong Yi ),이민영 ( Min Young Lee ),안현숙 ( Hyun Suk Ahn ),최민혜 ( Min Hye Choi ),성나영 ( Na Young Sung ),황유임 ( Yu Im Hwang ),송인옥 ( In Ok Song ),궁미경 ( Mi Kyoung Koong ),강인수 ( In 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.7

목적: 원인불명의 습관성 유산 환자에서 말초혈액 중 비정상적 비율로 증가된 자연살해세포가 자궁으로의 혈류를 감소시키는지에 대해 알아보고자 하였다. 연구방법: 본 연구는 전향적 연구로, 습관성 유산의 기왕력을 갖는 임신 5-7주 사이의 환자 33명을 대상으로 하였으며 습관성 유산의 기왕력이 없는 정상 임신 5-7주 47명을 대조군으로 하였고, 습관성 유산군은 말초혈액 자연살해세포 비율 12.1%를 기준으로 정상(normal) natural killer (NK) group과 증가(elevated) NK group으로 나누어 연구를 진행하였다. 임신 5-7주 사이에 측정한 말초혈액에서 유세포분석기를 이용하여 말초혈액 자연살해세포 분획 및 세포용해 활성도를 측정 후 그 결과를 비교 분석하였고 질식초음파의 Doppler를 이용하여 자궁방 사동맥의 저항을 측정 후 각 군에서 비교하였다. 또한 말초혈액 자연살해세포분획과 자궁방사동맥 저항지수 간의 연관성을 알아보았다. 결과: 자궁방사동맥의 평균 저항지수는 대조군에 비교하여 습관성 유산 환자군에서 통계학적으로 의미 있게 높았고(0.60 ± 0.14 vs. 0.54 ± 0.12, P = 0.039), 말초혈액 자연살해세포의 분획이 12.1% 이상 증가(elevated NK)군에서도 정상대조군에 비해 통계학적으로 유의하게 높게 나타났다(0.62 ± 0.13 vs. 0.54 ± 0.12, P = 0.029). 또한 전체 습관성 유산 환자에서 말초혈액 자연살해세포의 비율과 자궁방사동맥 저항지수 사이에는 양의 상관관계가 나타났고(P = 0.013, r = 0.429), 특히 습관성 유산 환자중 증가(elevated) NK군에서는 더욱 강한양의 상관관계를 보였다(P = 0.001, r=0.667). 결론 증가된 말초혈액의 자연살해세포는 자궁방사동맥과 같은 미세 혈관에 향염증 반응을 일으켜 자궁으로의 혈류를 감소시켜 습관성 유산을 야기한다고 설명할 수 있겠다. 하지만 본 연구의 결론을 확인하기 위해서는 더 큰 규모의 연구뿐 아니라 분자생물학적 기전에 대한 연구도 필요할 것으로 생각된다. Objective: To evaluate whether increased peripheral blood inflammatory immune cell can induce decrease of uterine blood flow in patients with unexplained recurrent spontaneous abortion (RSA). Methods: This study designed prospectively 33 pregnant women between 5 to 7 gestational weeks with a history of unexplained RSA included in this study. 47 normal pregnant women between 5 to 7 gestational weeks without history of infertility and/or RSA included as a control. Peripheral blood natural killer (pbNK) (CD3-/56+) fractions among peripheral blood monocyte (PBMC) were checked by flow cytometry. Uterine color-pulsed Doppler trans-vaginal ultrasound for evaluation of uterine radial artery RI was checked. Uterine radial artery resistance index (RI) compared between study and control group. After then, uterine radial artery RI was compared between high pbNK cell fraction above 12.1% among PBMC and normal pbNK cells fraction below 12.1%, Correlation between pbNK cell fraction to uterine radial artery RI was also evaluated, Results: Uterine radial artery RI in early pregnancy was significantly higher in patients with RSA than that of normal control (0.60 ± 0.14 vs. 0.54 ± 0.12, P = 0.039). Especially, the mean value of uterine radial artery RI in RSA patients with elevated pbNK cells was significantly increased than that of normal control (0.62 ± 0.13 vs. 0.54 ± 0.12, P=0.029). Otherwise, pbNK cell fractions among PBMC displayed strong positive correlation to uterine radial artery RI (Pearson`s correlation coefficient P=0.001, r = 0.667). Conclusion: Increased pbNK cells can evoke decreased uterine blood flow by their pro-inflammatory action on micro vascular structure such as uterine radial artery. This can be a one causative mechanism of inducing spontaneous abortion by increased NK cells. But, larger scaled study is needed for clarify our results.

18F-FDG uptake in breast cancer correlates with immunohistochemically defined subtypes

Koo, Hye Ryoung,Park, Jeong Seon,Kang, Keon Wook,Cho, Nariya,Chang, Jung Min,Bae, Min Sun,Kim, Won Hwa,Lee, Su Hyun,Kim, Mi Young,Kim, Jin You,Seo, Mirinae,Moon, Woo Kyung Springer-Verlag 2014 EUROPEAN RADIOLOGY Vol.24 No.3

벌꿀제품에 있어서 잔류항생물질에 관한 조사연구

구용의,강민철,이선화,김순한,김재이,김은정,김연희,유병옥,김주원,최계선,이향미,지영애,홍무기 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

꿀벌의 무저병을 치료하기 위하여 테트라싸이클린 계열의 항생물질(TCs)이 널리 사용되고 있으며,이러한 사용은 벌꿀중 Cs의 잔류문제를 일으킬 수 있다. 따라서 벌꿀중 잔류하는 TCs의 분석법이 팔요하며, 본 연구에서는 벌꿀클의 옥시테트라싸이클린(OTC), 테트라싸이클린fC), 클로르테트라싸이 클린(CTC), 독시싸이클린(DC)끌 분석하기 위해 두가지 HPLC방법을 사용하였다. 아카시아꿀, 잡화꿀,토종꿀, 방꿀등 4가지 종류의 댈꿀에 대한 회수율 및 검출한계를 측정하였다. 형광검출기를 이웅한 분석법에서는 0.Sppm의 수준에온 회수율은 OTC는 91.4~98.2%, TC는 84.B~94.4%, CTC는 뚬.I~105.9%, DC는 뽀.5~져.5%이었으며, 검출한계는 OTC와 TC는 0.01ppf CTC차 OC는 0.05ppm이었다. 자외선검출기를 이용한 분석법에퍼는 0.Sfpm의 수준에서 회수율은 OTC는 78.3~97.0%, TC는 76.8~86.8%, CTC는 89.6~99.5%, Df는 91.I~93.8%이었으며, 검출한계는 OTC와 TC는 0,01ppm, CTC와 DC는 0.02ppm이었다. 두 ITPLC법으로 국내산 벌꿀 140건과 외국산 벌꿀 29건을 조사한 결과 OTC,TC, CTC, BC 모두 검출되지 않았다. ln order to prevent foul brood of honey-bees, tetracycline antibiotics(TCs) are widely used in honey-bee culture. Such usage may resu)t in residues of TCs in honey.Therefore, the establishment of a method for residue analysis of TCs in honey is required.Two HPLC methods for oxytetracycline(OTC), tetracycline(TC), chlortetracycline(CTC) anddoxycycline(DC) in honey have been established. The recoverles of OTC, TC, CTC and BCfrom 4 kinds of honelr(acacia honey, mixed flower honey, native honey, chestnut honey) spikedat a level of 0.Sppm by HPLC with fluorescence detection are 91.4 ~98.2%, 84.B~94.4%, 85.1 ~105.9%, 86.5 ~94.5% refpectively. The limits of detection in honey b.# HPLC with fluorescencedetection are 0.01ppm for OTC and TC, and 0.05ppm for CTC and DC respectively. And therecoveries of OTC, TC, CTC and DC from 4 kinds of honey spiked at a level of 0,Sppm byHPLC with UV detection are 78.3 ~97.0%, 76,8 ~86.8%, 89.6 ~99.5%, 91,1 ~93,8% respectively.The Ihuts of detection in honey by HPLC with UV detection are 0.01ppm for OTC and TC,and 0.02ppm for CTC and DC respectively The residual TCs(OTC, TC, CTC, DC) were notde7ected in the 169 samples of honey(domestic honey 140, imported honey 29) by two HPLCmethods.