FET형 반도체 마이크로센서 개발(Ⅲ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,박재윤,송경은,김창수 경북대학교 센서기술연구소 1995 연차보고서 Vol.1995 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)센서소자 및 분석 시스템, 용존가스(O_2, CO_2)센서의 개발을 중점적으로 추진하였으며, FET형 압력센서, 습도센서 둥의 개별 FET형 센서에 관한 기초연구도 병행하였다. The conventional sensors have many problems such as high cost, large dimension, long analysis time and troublesome handling to apply to the fields of medical diagnosis, monitoring of chemical process and environmental monitoring/control. The main objects of this research are to develope a new FET type electrolyte(H^+, K^+, Ca^2+, Na^+)sensors, analysis system, and dissoved gas(O_2, CO_2)senors that can overcome the problems of the conventional sensors, and parallel basic researches on FET. type sensors such as pressure and humidity are also in progress.

유전자치료를 위한 벡터 개발의 연구 동향 : A Current Research Insight

손은화,손은수,표석능 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.5

The basic concept underlying gene therapy is that human diseases may be treated by the transfer of genetics material into specific cells of a patient in order to correct or supplement defective genes responsible for disease development. There are several systems that can be used to transfer foreign genetic material into the human body. Both viral and non-viral vectors are developed and evaluated for delivering therapeutic genes. Viral vectors are biological systems derived from naturally evolved viruses capable of transferring their genetics materials into host cells. However, the limitations associated with viral vectors, in terms of their safety, particularly immunogenecity, and their limited capacity of transgenic materials, have encouraged researchers to increasingly focus on non-viral vectors as an alternative to viral vectors. Although non-viral vectors are less efficient than viral ones, they have the advantages of safety, simplicity of preparation and high gene encapsulation capability. This article reviews the most recent studies highlighting the advantages and the limitation of gene delivery systems focused on non-viral systems compared to viral systems.

Effects of Non-saponin Red Ginseng Components on the Function of Brain Cells

Sohn, Eun-Hwa,Do, Hang,Kang, Nam-Sung,Jang, Seon-A,Park, Sul-Kyung,Lee, Hye-Rim,Rhee, Dong-Kwon,Pyo, Suhk-Neung The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.1

Non-saponin gingseng fraction components (NSRG) have been known to have a variety of biological activity. However, the effects of these components on the function of brain cell have not been characterized in detail. In this study, we investigated the preventive effect of non-saponin red ginseng components on acrylamide (ACR)-induced suppression of neural cell adhesion molecule (NCAM), which is highly expressed in neuronal cells. The data showed that NSRG blocked the suppression of NCAM expression by ACR in neuroblastoma cells (SK-N-SH). In addition, NSRG significantly increased NCAM expression in ACR-nontreated neuroblastoma cells. NSRG treatment resulted in the increase of cell proliferation in a concentration-dependent manner. We also examined whether NSRG could modulate the NO production of astrocytes. When glioma cells (C6) were treated with various concentrations of NSRG (100-300 ug/ml) in the presence or absence of $IFN-{\gamma}$ for 24 hours, NO production was suppressed in $IFN-{\gamma}-$stimulated C6 cells. Taken together, these results demonstrate that treatment of brain cells with NSRG results in the enhancement of proliferation, the suppression of NO production and the protective effect on NCAM expression impaired by ACR. Thus, the present data suggest that NSRG has proliferative and neuroprotective effects and these effects could be useful in neuronal diseases.

Development of Angiogenesis Inhibitors : an Analysis of the Patent Literatures

Eun-Soo Sohn,Eun-Hwa Sohn 대한의생명과학회 2011 Biomedical Science Letters Vol.17 No.2

The development of a general appreciation for the central role of angiogenesis in cancer growth and metastasis and other disease status has led to a wide range of new therapeutic strategies. This paper reviews the domestic and international trends through technology, marketing and patent information analysis dealing with anti-angiogenic agents. This analytical research has led to the identification of new targets associated with angiogenesis, leading to the development of an extensive number of preclinical screening of antiangiogenetic agents.

Phagocytic Effects of β-Glucans from the Mushroom Coriolus versicolor are Related to Dectin-1, NOS, TNF-α Signaling in Macrophages

( Eun Hwa Sohn ),( Seon A Jang ),( Se Chan Kang ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.4

The mushroom Coriolus versicolor contains biologically active polysaccharides, most of which belong to the β-glucan group. Diverse physicochemical properties, due to different sources and isolated types of β-glucans, can induce distinct biological activities. We investigated the effects of β-glucans from C. versicolor on phagocytic activity, nitric oxide (NO), TNF-α production, and signaling of dectin-1, a well-known β-glucan receptor, in macrophages. β-Glucans increased phagocytic activity and TNF-α and NO-iNOS/eNOS production. Laminarin, a specifi c inhibitor of dectin-1, showed strong inhibitory effects on phagocytosis and subsequent TNF-α, iNOS, and eNOS production increased by β-glucans, indicating that β-glucans reacts with dectin-1 receptors. We examined whether the aforementioned cytokines were involved in the signaling pathway from the dectin-1 receptor to phagocytosis, and found that the inhibition of iNOS, eNOS, and TNF-α receptors signifi cantly decreased β-glucan-induced phagocytosis. In conclusion, our study showed that dectin-1 signaling, triggered by β-glucans, subsequently elicited TNF-α and NO-iNOS/eNOS production, and that these molecules seem to act as secondary molecules that cause eventual phagocytosis by macrophages. These fi ndings suggest that C. versicolor could be used as a nutritional medicine that may be useful in the treatment of infectious disease.

Effects of Non-saponin Red Ginseng Components on the Function of Brain Cells

Eun-Hwa Sohn,Hang Do,Nam-Sung Kang,Seon-A Jang,Sulkyung Park,Hyerim Lee,Dong-Kwon Rhee,Suhkneung Pyo 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

Non-saponin gingseng fraction components (NSRG) have been known to have a variety of biological activity. However, the effects of these components on the function of brain cell have not been characterized in detail. In this study, we investigated the preventive effect of non-saponin red ginseng components on acrylamide (ACR)-induced suppression of neural cell adhesion molecule (NCAM), which is highly expressed in neuronal cells. The data showed that NSRG blocked the suppression of NCAM expression by ACR in neuroblastoma cells (SK-N-SH). In addition, NSRG significantly increased NCAM expression in ACR-nontreated neuroblastoma cells. NSRG treatment resulted in the increase of cell proliferation in a concentration-dependent manner. We also examined whether NSRG could modulate the NO production of astrocytes. When glioma cells (C6) were treated with various concentrations of NSRG (100-300 ㎍/ml) in the presence or absence of IFN-γ for 24 hours, NO production was suppressed in IFN-γ-stimulated C6 cells. Taken together, these results demonstrate that treatment of brain cells with NSRG results in the enhancement of proliferation, the suppression of NO production and the protective effect on NCAM expression impaired by ACR. Thus, the present data suggest that NSRG has proliferative and neuroprotective effects and these effects could be useful in neuronal diseases.

Immunomodulatory Effects of Non-saponin Red Ginseng Components on Innate Immune Cells

Eun-Hwa Sohn,Jihye Kim,Hyesook Choi,Hye-Jin Park,Byung-Oh Kim,Dong-Kwon Rhee,Suhkneung Pyo 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

Macrophages and NK cells play an important role in the first line of immunologic effects against tumor cells. We therefore assessed the effects of non-saponin red ginseng components (NSRG) on NK cell and macrophage tumoricidal activities, and the mitogen-stimulated lymphoproliferative response. When NK cells were treated with various concentrations of NSRG (100-300 ㎍/ml) for 4 h, tumoricidal activity was significantly increased. However, NSRG had effects on tumoricidal activity of macrophages at low concentration (1 ㎍/ml), which was not related to the production of nitric oxide. The mitogen response of lymphocytes to LPS and ConA in the spleen did not show significant differences between the control and NSRG-treated cells, whereas LPS-induced blastogenesis was slightly increased at 100 ㎍/ml (p < 0.05). These results suggest that NSRG has differential effects on innate immune response and could be useful as immunotherapy for cancer treatment.

Effects of Korean Red Ginseng Extract for the Treatment of Atopic Dermatitis-Like Skin Lesions in Mice

Sohn, Eun-Hwa,Jang, Seon-A,Lee, Chul-Hoon,Jang, Ki-Hyo,Kang, Se-Chan,Park, Hye-Jin,Pyo, Suhk-Neung The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.4

Atopic dermatitis (AD) is an allergic, inflammatory skin disease characterized by chronic eczema and mechanical injury to the skin, caused by scratching. Korean red ginseng (RG) has diverse biological activities, but the molecular effects of RG on allergic diseases, like AD, are unclear. The present study was designed to investigate whether RG inhibits 1-chloro-2,4-dinitrobenzene (DNCB)-induced AD in a mouse model. DNCB was applied topically on the dorsal surface of Balb/c mice to induce AD-like skin lesions. We observed the scratching behavior and examined the serum IgE level and interleukin (IL)-4 and IL-10 in splenocytes compared with dexamethasone. We also evaluated the DNCB-induced mitogen-activated protein kinases (MAPKs), NF-${\kappa}B$, and Ikaros activities after RG treatment using reverse transcriptase-polymerase chain reaction, Western blotting, and ELISA. Our data showed that the topical application of RG significantly improved the AD-like skin lesions and scratching behavior. RG decreased not only the mRNA expression of IL-4 and IL-10, but also the secretion of IL-4 protein and serum IgE in mice. Additionally, RG treatment decreased the DNCB-induced MAPKs activity and subsequent Ikaros translocation irrespective of NF-${\kappa}B$. We suggest that RG may be useful as a therapeutic nutrition for the treatment of AD.

Effects of Korean Ginseng and Wild Simulated Cultivation Ginseng for Muscle Strength and Endurance

Sohn, Eun-Hwa,Yang, Yoon Jung,Koo, Hyun Jung,Park, Dae Won,Kim, Ye-Jin,Jang, Ki Ho,NamKoong, Seung,Kang, Se Chan The Plant Resources Society of Korea 2012 한국자원식물학회지 Vol.25 No.6

Muscle strength and endurance activities of Korean ginseng (Panax ginseng C. A. Meyer; KG) were compared with those of wild simulated cultivation ginseng (WCG) in mice. Fifty male ICR mice were divided into five groups: A (vehicle); B (WCG 100 mg/kg); C (WCG 500 mg/kg); D (KG 100 mg/kg); E (KG 500 mg/kg). Subsequently, the mice were subjected to the forced swimming test (FST) and treadmill test at the $4^{th}$ and $7^{th}$ weeks. The glycogen content in the muscle and blood analysis (levels of glucose, triglyceride (TG), IGF-1) were also performed immediately after the last FST and treadmill test at the $7^{th}$ week. Immobility times in FST were shorter in WCG- than KG-treated groups, and the results of the treadmill tests were also significant except for KG-treated at 100 mg/kg. The glycogen content was increased in both groups with a peak at 500 mg/kg of WCG groups. Serum concentrations of TG and glucose were decreased by administration of KG and WCG and all treated groups showed increase in the level of IGF-1 in serum. These results suggest that KG and WCG supplementations are effective in escalating the muscle strength and endurance.

Immunomodulatory Effects of Non-saponin Red Ginseng Components on Innate Immune Cells

Sohn, Eun-Hwa,Kim, Ji-Hye,Choi, Hye-Sook,Park, Hye-Jin,Kim, Byung-Oh,Rhee, Dong-Kwon,Pyo, Suhk-Neung The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.1

for 4 h, tumoricidal activity was significantly increased. However, NSRG had effects on tumoricidal activity of macrophages at low concentration (1 ug/ml), which was not related to the production of nitric oxide. The mitogen response of lymphocytes to LPS and ConA in the spleen did not show significant differences between the control and NSRG-treated cells, whereas LPS-induced blastogenesis was slightly increased at 100 ${\mu}g/ml$ (p < 0.05). These results suggest that NSRG has differential effects on innate immune response and could be useful as immunotherapy for cancer treatment.