Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

Han Sang-soo; Using Needle and Thread to Create Exquisite Embroidery

Lee Seong-soo Poet Seo Heun-kang Photographer The Korea Foundation 2004 Koreana (English) Vol.18 No.1

Curcumin-induced Histone Hypoacetylation Enhances Caspase-3-dependent Glioma Cell Death and Neurogenesis of Neural Progenitor Cells

Kang, Soo-Kyung,Cha, Seung-Heun,Jeon, Hyo-Gon Mary Ann Liebert 2006 STEM CELLS AND DEVELOPMENT Vol.15 No.2

<P>Acetylation of histones and nonhistone proteins is an important post-translational modification involved in the regulation of gene expression in mammalian cells. Dysfunction of histone acetyltransferase (HAT) is often associated with the manifestation of several diseases. In this report, HATs are new targets for the development of therapeutics. Our studies first proved that curcumin induces histone hypoacetylation in brain cancer cells and finally induces apoptotic cell death through a (PARP)- and caspase 3-mediated manner. In addition, curcumin induces recontrolling of neural stem cell fates. It induces effective neurogenesis, synaptogenesis, and migration of neural progenitor cells in vitro in brain-derived adult neural stem cells. We also confirmed the neurogenic effect of curcumin in our in vivo experiments. Curcumin actively suppressed differentiation in astrocytes while promoting differentiation into the neurons associated with decrease of histone H3 and H4 acetylation. We suggest that histone hypoacetylation plays an important role in determine stem cell fate through controlling the simultaneous expression of many genes. Thus, the present finding that curcumin, a nontoxic dietary compound, is a histone acetyltransferase inhibitor would supply a new window to understand further the molecular mechanism of histone acetylase inhibitors (HAI) in cancer and neural stem cells and provide a new target molecule for treating central nervous system disorders.</P>

Inhibition of Inducible Nitric Oxide Synthase Expression by YS 49, a Synthetic Isoquinoline Alkaloid, in ROS 17/2.8 Cells Activated with $TNF-{\alpha},\;IFN-{\gamma}$ and LPS

Kang, Young-Jin,Kang, Sun-Young,Lee, Young-Soo,Park, Min-Kyu,Kim, Hye-Jung,Seo, Han-Geuk,Lee, Jae-Heun,YunChoi, Hye-Sook,Chang, Ki-Churl The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.5

Nitric oxide (NO) has been suggested to act as a mediator of cytokine-induced effects of turn over of bone. Activation of the inducible nitric oxide synthase (iNOS) by inflammation has been related with apoptotic cell death in osteoblast. YS 49, a synthetic isoquinoline alkaloid, inhibits NO production in macrophages activated with cytokines. In the present study, we investigated the molecular mechanism of YS 49 to inhibit iNOS expression in ROS 17/2.8 cells, which were activated with combined treatment of inflammatory cytokines $(TNF-{\alpha},\;IFN-{\gamma})$ and lipopolysaccharide (LPS). Results indicated that YS 49 concentration-dependently reduced iNOS mRNA and protein expression, as evidenced by Northern and Western blot analysis, respectively. The underlying mechanism by which YS 49 suppressed iNOS expression was not to affect iNOS mRNA stability but to inhibit activation and translocation of $NF-_kB$ by preventing the degradation of its inhibitory protein $I_kB_{\alpha}$. As expected, YS 49 prevented NO-induced apoptotic cell death by sodium nitroprusside. Taken together, it is concluded that YS 49 inhibits iNOS expression by interfering with degradation of phosphorylated inhibitory $_kB_{\alpha}\;(p-I_kB_{\alpha})$. These actions may be beneficial for the treatment of inflammation of the joint, such as rheumatoid arthritis.

백서 해마에서 카이닌산 유도 경련에 의한 JNK 경유 신호전달경로 활성화의 발달 단계에 따른 변화

김종흔,정희연,노명선,안용민,강웅구,김용식,조수철 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 어린 백서의 해마에서 전기경련충격(electroconvulsive shock, ECS) 후 활성화되는 것으로 알려져 있는 MAPK계 신호전달경로가 카이닌산(Kainic acid, KA)에 의한 경련 후 어떤 양상으로 활성화되는 지를 초기 발달 단계에 따라서 관찰하고자 하였다. 방 법 : 생후 7일, 14일, 21일 된 어린 백서와 성년 백서의 해마에서 면역블롯 방법으로 MAPK계 효소의 인산화 효소 중 하나인 SAPK/ERK kinase-1(SEK-1), MAPK계 효소인 c-Jun N terminal protein kinase(JNK), 전사인자 c-Jun 및 조기유전자 단백질 Fos의 단백질 양과 기저 인산화를 측정하였고, 백서에게 KA를 주사하여 경련을 유발한 후 위 단백질들의 인산화 변화의 양상을 같은 방법으로 관찰하였다. 결 과 : SEK-1, JNK, c-Jun 모두 연령 증가에 따른 단백질 양 자체에는 변화가 없었다. JNK와 c-Jun은 기저 인산화의 양적 변화도 없었으나 SEK-1의 경우 기저 인산화 양이 생후 7일에 가장 높고 이후 연령이 높아짐에 따라 감소하였다. JNK의 경우 KA 주사 후 인산화 변화 양상이 관찰되지 않았으나 SEK-1과 c-Jun은 생후 14일부터 연령의 증가와 함께 인산화 양도 증가하였다. Fos 역시 생후 7일부터 발현되어 연령의 증가와 함께 발현양이 증가하였다. 결 론 : 백서 해마에서의 MAPK계 신호전달계의 성숙은 연령의 증가에 따라, 또 신호전달 물질에 따라 차이를 보였다. KA 유도 경련 후 JNK는 생후 21일까지도 활성화가 되지 않는 것으로 보아, 세포주에서 알려진 SEK-1 - JNK-c-Jun - Fos로 연결되는 신호전달 경로가 어린 백서의 해마에서는 적용될 수 없음을 시사한다. Objective : We observed the developmental pattern of activation of MAPK signal transduction pathways known to be activated by eletroconvulsive shock(ECS) in young rat hippocampus after kainic acid(KA)-induced seizure. Methods : We used the method of immunoblotting for examining the basal protein amount and basal level of phosphorylation of MAPK kinase(SAPK/ERK kinase -1, SEK-1), MAPK(c-Jun N terminal protein kinase, JNK), transcription factor(c-Jun) and immediate early gene proteins(Fos) in rat hippocampus at postnatal day 7, 14 and 21, respectively. We also examined the changes of phosphorylation of those proteins after kainic acid-induced seizure in the same way. Results : The basal protein amounts of SEK-1, JNK, and c-Jun did not show age-dependent changes and basal level of phosphorylation of JNK and c-Jun remains unchanged throughout the early developmental period. The basal level of phosphorylation of SEK-1 was peaked at postnatal 7 days and then decreased with aging. After kainic acid-induced seizure, the change of phosphorylation of JNK was not observed but those of SEK-1 and c-Jun increased after postnatal day 14. The expression of Fos was observed at postnatal day 7 and also increased with aging. Conclusion : These results show that the MAPK signal transduction system in rat hippocampus matures in accordance with aging, but the process of maturation differs depending specific proteins. This study suggests the signal transduction cascade(SEK-1 - JNK - c-Jun - Fos) which is well established in cell line studies may not be applied to rat hipposcampus because we could not observe the activation of JNK after KA-induced seizure in young rat hippocampus.

Inhibition of Inducible Nitric Oxide Synthase Expression by YS 49, a Synthetic Isoquinoline Alkaloid, in ROS 17/2.8 Cells Activated with TNF-α, IFN-γ and LPS

Young Jin Kang,Sun Young Kang,Young Soo Lee,Min Kyu Park,Hye Jung Kim,Han Geuk Seo,Jae Heun Lee,Hye Sook Yun-Choi,Ki Churl Chang 대한생리학회-대한약리학회 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.5

Nitric oxide (NO) has been suggested to act as a mediator of cytokine-induced effects of turn over of bone. Activation of the inducible nitric oxide synthase (iNOS) by inflammation has been related with apoptotic cell death in osteoblast. YS 49, a synthetic isoquinoline alkaloid, inhibits NO production in macrophages activated with cytokines. In the present study, we investigated the molecular mechanism of YS 49 to inhibit iNOS expression in ROS 17/2.8 cells, which were activated with combined treatment of inflammatory cytokines (TNF-α, IFN-γ) and lipopolysaccharide (LPS). Results indicated that YS 49 concentration-dependently reduced iNOS mRNA and protein expression, as evidenced by Northern and Western blot analysis, respectively. The underlying mechanism by which YS 49 suppressed iNOS expression was not to affect iNOS mRNA stability but to inhibit activation and translocation of NF-κB by preventing the degradation of its inhibitory protein IκBα. As expected, YS 49 prevented NO-induced apoptotic cell death by sodium nitroprusside. Taken together, it is concluded that YS 49 inhibits iNOS expression by interfering with degradation of phosphorylated inhibitory κBα (p-IκBα). These actions may be beneficial for the treatment of inflammation of the joint, such as rheumatoid arthritis.

유방에 발생한 다발성 결정성 동맥성 1예

박찬흔(Chan Heun Park),강구(Koo Kang),배수동(Soo Tong Pai) 대한외과학회 1991 Annals of Surgical Treatment and Research Vol.41 No.4

Effects of GABAergic Drugs on the Neuronal Activity of Trigeminal Spinal Sensory Nucleus In Vitro

Lee, Cheol-Hee,Kang, Hyo-Shik,Kim, Joong-Soo,Lee, Jong-Heun The Official Publication of Korean Academy of Oral 1993 International Journal of Oral Biology Vol.17 No.2

The effects of GABAergic drugs on trigeminal spinal sensory nucleus were studies in vitro. Transverse slices of caudal part of trigeminal sensory nucleus were prepared and perfused with aerated modified Krebs-Ringer solution. Intracellular recordings were carried out with glass microelectrode to record the changes of membrane potential and membrane input resistance induced by GABA, baclofen, and bicuculline and glutamate perfusion. GABA and baclofen, a GABA_B agonist, did not influence significantly on the membrane potential and membrane input resistance. Bicuculline, a GABA_A antagonist, depolarized medullary dorsal horn neurons but lacked effects on membrane input resistance. Depolarizing effect of glutamate on medullary dorsal horn neurons was increased by simultaneous application of bicuculline.

물리적인 방법을 이용한 잡초 및 병해충 방제 방법의 개발 : 적외선 조사가 잡초종자의 발아율에 미치는 영향 Effect of infrared irradiation on viability of weed seeds

유창연,강화석,강위수,이귀현,오재헌 한국환경농학회 1996 한국환경농학회지 Vol.15 No.1

본 연구는 LPG를 사용한 방열장치를 이용하여 잡초종자를 열처리한 후 처리온도간에 차이가 있다는 판단하에 잡초종자의 발아력에 관계하는 잡초의 종류, 잡초종자의 상태, 조사시간을 실험의 변수로 선택하여 그 주효과 및 각 변수간의 상호작용에 대한 효과를 분석하여 열처리 잡초방제 방법 개발에 필요한 기초자료를 제공하고자 하였다. 실험에 이용된 잡초종자는 화본과인 바랭이(Digitaria sanguinalis S.)와 쌍자엽잡초인 쇠비름(Portulaca oleracea L.)의 건조종자 및 습윤종자(증류수에 24시간 침지)를 사용하였고, 5개의 온도 수준과 8개수준의 조사시간으로 각 종자를 똑같이 처리하였으며 각 처리별로 3회 반복하였다. 본 실험의 결과를 요약하면 다음과 같다. 1) 각 변수에 대한 잡초종자의 발아력은 처리 온도가 150℃일 때를 제외하고 평균 발아율이 모두(1% 유의수준) 차이가 있는 것으로 분석되었다. 잡초종자의 처리상태에 대해서는 평균 발아율이 60℃에서는 유의성이 없었으며 80℃, 100,℃, 200℃에서는 1%의 유의수준에서 차이가 있는 것으로 분석되었고 150℃에서는 5%의 유의수준에서 차이가 있는 것으로 나타났다. 2) 조사시간에 따른 잡초의 평균발아율은 모든 처리온도 수준에서 차이가 있는 것으로 분석되었고(1% 유의수준), 모든 온도수준에서 잡초의 종류와 조사시간 사이에 상호작용이 있는 것으로 나타났다(1%유의수준). 3) 잡초의 처리상태와 조사시간 사이에도 역시 각 온도수준에서 차이가 있는 것으로 나타났고(1% 유의수준), 잡초의 종류와 잡초의 처리상태 간에는 60℃를 제외한 모든 온도수준에서 차이가 있는 것으로 나타났다(1% 유의수준). 4) 잡초의 평균발아율은 각 온도 수준에서 조사 시간에 따라 가장 큰 영향을 받는 것으로 분석되었다. This study was to provide the basic information for the development of thermal weeder which uses LPG as fuel. Weed seeds of Digitaria sanguinalis S. and Portulaca oleracea L. mainly developed in from and forestry nurseries were used as experimental samples. At different irradiation temperature(60, 80, 100, 150, 200℃), the dffects of weed seed species(digitalis, purslane), condition of seed(dry, soaked), and irradiation time(2, 5, 10, 20, 30, 60, 180, 300 sec) on seed viability were investigated was investigated by examining interaction and main effect of experimental factors. The results showed that viability of weed seed was significantly affected by all irradiation temperature tested. Irradiation time significantly affected on viability of weed seed on all levels of irradiation temperature. also, there were interactions between condition of weed seed and irradiation time on seed viability at each irradiation temperature.

Neuroprotective Effect of Platycarya Strobilacea Infructescence Extract on Transient Focal Cerebral Ischemia in mice

Ji Hye Lee,Ji Heun Jeong,Jong Hun An,Ji Hyun Moon,Seong Hee Kang,Eun Soo Do,Do-Kyung Kim,Nam-Seob Lee,Young-Gil Jeong,Seung Yun Han 대한체질인류학회 2018 대한체질인류학회 학술대회 연제 초록 Vol.61 No.-