경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

피로한도의 정량평가법과 구상흑연주철에 대한 적용

김진학,김민건 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Fatigue tests were performed to examine the 4 parameter method for specimens prepared by various heat treatment which resulted in different mechanical properties. Obtained main results are as follows. Fatigue tests were performed to examine the 4 parameter method for specimens prepared by various heat treatment which resulted in different mechanical properties. Obtained main results are as follows. (1) Samples treated by austempering did not show the expected improvement of fatigue limit although hardness and strength increased. This is attributed to the fact that defect sensitivities of materials increase as increasing of hardness and tensile strength, it is also shown that the graphites acting as stress concentration place become larger by austempering heat treatment than by normal annealing. (2) It is very reasonable to predict the fatigue limit of ductile irons with 4 parameter method based on σ?, H?, ΔK? and √area ?. (3) The half-austempering treatment appeared to be more useful than the full-austempering method to improve the fatigue limit in the spheroidal graphite cast iron with multi defective material.

비소세포폐암에서 동시화학방사선요법을 받는 중 생긴 기관폐루1례

강건희,조성우,김성록,유영진,이효락 인제대학교 2008 仁濟醫學 Vol.29 No.-

최근 근치적 절제가 불가능한 국소진행성 비소세포 폐암에서 화학요법을 방사선요법과 동시에 투여하는 치료방법에 대한 연구가 활발이 이루어지고 있으며 이러한 치료과정 중 생긴 여러 부작용에 대한 연구도 이루어지고 있지만 아직까지 기관폐루가 생겼다고 보고는 없다. 따라서 치료과정 중 기관폐루가 의심되는 소견이 있을 경우 이에 대한 조기 진단과 치료를 통하여 부작용에 따른 사망률을 최소화하여야 하겠다. The incidence of lung cancer is increasing steadily and various methods have been attempted to enhance the cure rate of locally advanced non small cell lung cancer, which is hardly removable surgically, but the results are not satisfactory yet. Recently, research is being made actively on the simultaneous application of chemotherapy and radiotherapy for locally advanced non small cell lung cancer that cannot be radically resected and the we experienced a case of patient diagnosed with bronchopulmonary fistula during the treatment process, and therefore report it here.

Panipenem을 포함한 Carbapenem 계열 항생제의 몇 가지 임상균주에 대한 시험관내 항균력

이동건,김명신,강진한,전혜선,최수미,위성헌,김상일,최정현,유진홍,신완식,강문원 대한감염학회 2003 감염과 화학요법 Vol.35 No.2

목적 : Panipenem은 일본에서 개발된 카바페넴계 항생제로 그람양성균, 그람음성균, 혐기균에 이르기까지 광범위한 항균력을 나타낸다고 보고되고 있지만 국내에서 분리되는 균주에 대한 감수성 검사결과는 아직 없다. 본 연구에서는 임상에서 분리된 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae 균주를 대상으로 carbapenem 계열 및 몇 가지 항생제와 감수성을 비교하고, 특히 P. aeruginosa의 경우 carbapenem의 효과를 Muller-Hinton agar (MHA)가 반감시킬 수 있는 특성을 고려하여 감수성에 변화가 있는지 확인하고자 하였다. 방법 : 2002년 3월부터 6월까지 가톨릭대학교 성모병원, 강남성모병원, 성가병원, 성모자애병원, 성빈센트병원에서 분리된 임상균주 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae를 대상으로 하였다. 항생제 감수성 검사는 NCCLS 기준에 따랐고 P. aeruginosa의 경우 MHA 이외에 minimal agar Davis (MAD)를 추가로 사용하였다. 감수성 결과 해석시 panipenem은 imipenem과 같은 기준을 적용하였다. 결과 : E. coli, K. pneumoniae, P. aeruginosa는 각각 100균주였고, S. penumoniae는 134균주였다(PSSP 41, PNSP 93균주). E. coli, K. pneumoniae는 모든 균주가 imipenem, meropenem, panipenem에 대해 감수성을 보였다. P. aeruginosa의 경우 imipenem, meropenem, panipenem, ceftazidime의 MIC_(90)은 각각 16, 8, 32, 64㎍/mL이었다. MHA와 MAD 배지를 이용하여 P. aeruginosa에 대한 항생제 감수성을 비교해 본 결과 imipenem, panipenem의 MIC_(90)가 각각 16, 32㎍/mL에서 8㎍/mL로 감소하였고, imipenem에 비해 panipenem이 더 많이 감소하였다. S. pneumoniae의 경우 PSSP는 imipenem, meropenem, panipenem에 대해 모두 내성이 없었고 PNSP는 imipenem, meropenem, panipenem에 대한 MIC_(90)가 각각 1, 2, 0.25㎍/mL이었다. 결론 : 결론적으로 panipenem은 E. coli, K. pneumoniae에 대해 기존의 카바페넴계 항생제와 유사한 우수한 항균력을 보였다. P. aeruginosa의 경우 ceftazidime, imipenem과 비슷한 항균력을 보였고 아미노산 함량이 적은 배지를 사용함에 따라 MIC가 낮아졌으며 imipenem보다 더 많은 영향을 받았다. S. pneumoniae의 경우 특히 PNSP에서 기존의 카바페넴계 항생제보다 우수한 항균력을 보였다. Panipenem은 주요 그람음성균 및 폐렴연쇄구균 감염에 유용할 것으로 기대된다. Background : Panipenern (PAPM) is a new carbapenern which has an enhanced broad spectrum against both gram-positive and negative organisms. The aim of study was to compare the activities of PAPM with those of imipenern (IMPM), meropenern (MRPM) against several clinical isolates in Korea. Methods : We tested the in vitro antimicrobial activities of PAPM, IMPM, and MRPM against total 300 clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa and 134 Streptococcus pneumoniae collected from 5 different university hospitals. Using NCCLS guidelines, MICs of PAPM, IMPM, MRPM, and/or ceftazidime were determined. Results : All isolates of E. coli and K. pneumoniae were susceptible to PAPM, IMPM and MRPM. MIG_(90) of PAPM, IMPM, and MRPM against P. aeruginosa were 32, 16, and 8 ㎍/mL, respectively. Comparing Mueller-Hinton agar (MHA) and minimal agar Davis (MAD), the MIG_(go) of PAPM and IMPM were reduced from 16 and 32 ㎍/mL to 8 and 8 ㎍/mL, respectively. PAPM was more influenced by MAD than IMPM. All isolates of penicillin susceptible S. pneumoniae showed 0% of resistance to the carbapenems tested. MIC_(90) of PAPM, IMPM and PRPM against penicillin non-susceptible S. pneumoniae were 0.25, 1 and 2 ㎍/mL, respectively. Conclusion : Panipenern could be one of the potentially useful drugs for the treatment of infections caused by E. coli, K. pneumoniae, and S. pneumoniae. We also showed that PAPM had good anti-pseudomonal activity when examined in MAD, the amino acid-limited media. Therefore, it could be useful for the treatment of infections caused by P. aeruginosa.

SM570 QT강 다층용접부에서 저온 균열방비를 위한 예열온도에 대한 연구

고진현,김건형 한국기술교육대학교 2006 論文集 Vol.13 No.1

A preheat temperature to prevent the cold cracking of SM570 QT structural steel weldments made by depositing multiple weld passes in the V-groove with FCAW was evaluated. The diffusible hydrogen content in the deposited weld metals increased linearly with increasing the hydrogen content in the shielding gas. It was confirmed that the cold cracking occurred in the weld specimens with preheat temperature at 50℃ and shielding gas of Ar+20%CO_(2) regardless of hydrogen content. On the other hand, the cracking did not occur in the wledments made with preheat and interpass temperatures at 75℃ and 100℃. Based on this study, it is suggested that the minimum preheat temperature to avoid the cold cracking for SM570 QT steel weld metal is 75℃.

시험관내 심내막염 모델에서 Methicillin 내성 포도구균에 대한 Vancomycin, Arbekacin, Gentamicin 단독 혹은 병합효과

이동건,전혜선,임동석,최수미,최정현,유진홍,신완식,강문원 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : Methicillin 내성 포도구균(methicillin resistant Staphylococcus aureus, MRSA)은 병원감염의 중요한 원인균으로 vancomycin이 선택약제이다. 그러나 심내막염 등의 중증감염에서는 vancomycin의 치료실패와 함께 최근 내성균도 증가하고 있어 vancomycin을 대체할 수 있는 항균제와 보다 효과적인 항균요법의 개발이 필요하다. 본 연구에서는 사람의 환경과 유사한 시험관내 감염 모델을 적용하여 MRSA 치료를 위한 vancomycin, arbekacin, gentamicin의 단독 및 병합효과를 비교하였다. 방법 : 임상에서 분리된 MRSA 2균주(GRI 53, gentamicin 내성 : GS171, gentamicin 감수성)로 fibrin clot을 제작하여 시험관내 심내막염 모델을 만들어 vancomycin, arbekacin, gentamicin을 단독 혹은 병합주입하였다. 반감기와 최고 및 최저농도는 사람의 약동학을 기준으로 하였다. Vancomycin은 6, 12, 24시간마다 주입하거나 24시간 연속주입하였고 arbekacin은 12, 24시간마다, gentamicin은 8, 24시간마다 주입하였다. 병합주입의 경우 vancomycin은 12시간마다, gentamicin, arbekacin은 24시간마다 투여하였다. 각 투여군의 살균효과는 0, 8, 24, 32, 48, 72시간째의 fibrin clot내 집락수로 측정하였다. 결과 : GRI153에 gentamicin을 주입한 군 이외의 모든 실험군에서 대조군에 비해 의미있게 fibrin clot내 집락수가 감소하였다(P<0.001). Vancomycin과 arbekacin 단독주입군내서 8시간째의 집락수는 GRI153보다 GS171에서 의미있게 낮았고(P=0.02), GS171에 arbekacin 12시간마다 투여한 군의 집락수가 가장 낮았다(P=0.01). 72시간째 집락수는 균주의 종류나 vancomycin 혹은 arbekacin의 투여간격에 따른 차이가 없었다. GRI153에서 vancomycin과 arbekacin의 병합주입군은 24시간까지 상가작용이 있었으나 vancomycin과 gentamicin 병합주입에서는 무관작용이었고 GS171에서도 72시간 동안 무관작용을 보였다. 또한 전 실험기간 동안 내성균은 발현되지 않았다. 결론 : 시험관내 심내막염 모델에서 MRSA에 대한 arbekacin의 항균력은 vancomycin과 유사하여 단독주입이 가능함을 시사한다. Gentamicin 내성균주의 경우 두가지 항균제의 병합주입으로 24시간까지 상가작용을 보이고 있으나 임상에 적용하기 위해서는 추가연구가 필요할 것으로 생각된다. Background : Glycopeptide has been used for the one-and-only treatment of choice in methicillin resistant Staphylococcus aureus (MRSA) infection, but its exclusive use for the MRSA infection has led to the increased risk of glycopeptide-resistance. To find an alternative (s), we employed an in vitro infective endocarditis model (IVIEM) to compare the efficacy of vancomycin (VCM). arbekacin (ABK), and gentamicin (GM) alone or in combination. Methods : Using two strains of clinically isolated MRSA, one GM susceptible (GS171) and the other GM resistant (GR153), fibrin clots were prepared and suspended in IVIEM. Antibiotics were added as a bolus to simulate human pharmacokinetics of regimens, including q 6 h, q 12 h, q 24 h, or continuous infusion with VCM, q 12h or q 24 h with ABK, and q 8 h or q 24 h with GM. In cases of combination, regimens were VCM q 12 h plus ABK q 24 h, and VCM q 12 h plus GM q 24 h. Fibrin clots were removed from each model at 0, 8, 24, 32, 48. and 72 h, and the bacterial densities (in CFU/g) were determined. Results : At 8 hour, the colony counts of GS171 were lower than those of GR153 (P=0.02), and the lowest with the ABK q12h against GS171 (P=0.01). At 72 hour, monotherapy with ABK or VCM produced same degree of bacterial reductions in IVIEM, regardless of dosing frequency or GM-resistance. In the case of GM-resistance, combination of VCM and ABK did show additive effect until 24 hours, although VCM and GM showed no indifference during all the experiments. Development of resistance during experiment was not observed with any regimens. Conclusions : Our data suggest that ABK monotherapy could be used as an alternative to VCM even in the treatment of GM-resistant staphylococcal endocarditis. Further studies will clinical trials are warranted to evaluate the additive effect of VCM and ABK.

직업전문학교 관광계열전공 학생들의 직업관과 진로의식에 관한 연구 : 서울 소재 직업전문학교를 중심으로

강진희,김건 문화관광연구학회 2003 문화관광연구 Vol.5 No.2

The purpose of this study is to investigate the vocational Training institute student’s perceptions of occupations and Perceptions and Course Consciousness to provide the guidelines and directions for the tourism department of the Vocational Training Institute in Seoul. Base on the result of review of literature and survey, the study presented the basic directions and plans of the education to improvement in vocational Training Institute student’s as follows. The student's perceptions of occupations hope to the tourism industry with there interest of character. and then, there are hope to establish about the text book for the perceptions of occupations. Enforce the education of vocational Training Institute view of the service industry to satisfy the both the students and industry’s needs.

Aspergillus niger로 부터 α-glucosidase 발현억제 형질전환체의 분리

이동건,이진영,서영배 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

We have already cloned an extracellular α-glucosidase gene from Aspergillus niger with oligonucleotide probe synthesized on the basis of the peptide sequences determined previously. The DNA sequence revealed an open reading frame of 895 amino acids split by three introns. We are attempting to construct an A. niger strain deficient in the α-glucosidase enzyme activity, which would be useful for the glucoamylase production without contamination by the industrially undesirable α-glucosidase. For destruction of the α-glucosidase gene, we try to make transformations. A cloned partial α-glucosidase gene was introduced into Aspergillus niger, and transformants with suppressed α-glucosidase activity were isolated. The transformants were cultured on YPD medium which contained Hygromycin B at 30℃. The activity of α-glucosidase of the suppressed transformants was compared to that of wild type activity. As shown by southern-hybridization, we detected that the transformant was a heterocaryon.

段 가공을 한 SM20C 異徑材 摩擦熔接部의 機械的 性質에 關한 硏究

김진건,강성보,민택기 충남대학교 공업교육연구소 1998 論文集 Vol.21 No.1

This study deals with mechanical properties in friction welding of SM20C steel bars and pipes which have different overhang lengths(0mm∼5mm). The experimental results m summarized as follows; In case of pipe, we can conclude that 1mm overhang is optimal condition for the products which needs the tensile and bending strength. But in case of bar, we can conclude that 0mm overhang is optimal condition for the Products which needs the tensile and bending strength.

안료-수지-용제간의 상호작용이 TiO_2 안료의 분산에 미치는 영향

이근대,홍성수,박진환,안병현 釜慶大學校 2000 釜慶大學校 論文集 Vol.5 No.-

The effect of pigment-resin-solvent interaction on dispersion of pigment has been studied. The solubility parameter of TiO_2 pigment, determined in terms of suspension behavior of pigment in various solvents, was 10.27 (cal/cc)^1/2(δ_d = 8.23, δ_p = 3.45, δ_h = 5.10). The solubility parameter of Acryloid B-44, determined using rheological parameters of resin solution in different solvent, was 9.4 (cal/cc)^1/2. The solvent showing weaker interaction with pigment increased the adsorption of resin on to the pigment, resulting in higher dispersibility and higher storage stability of dispersion.