Burkholderia cepacia를 이용한 축산폐수의 처리

정팔진,조선영,현미희,김민정,이은주,전민식 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

We found that the Bacterium Burkholderia cepacia in livestock wastewater treatment plant was predominant species. We investigated the growth rate of this and treatment characteristics for organic matter and nitrogen removal in livestock wastewater using this microorganism. First, we cultured B. cepacia. And then, to conducted treatment for livestock wastewater by using B. cepacia., we changed C/N from 0.2~4.4. When we operated A and B process, changing F/M ratio from 1.2 to 4.4. In experiment of C/N variations, when C/N was 1.8, we found that the optimal condition for organic matter and nutrient removal effect was higher and the removal efficiency of SCOD_(cr), SBOD_5, NH₄-N was 78.4%, 95% and 74.8%. So, It is possible to treat the wastewater having the lower C/N contents such as livestock wastewater using this microorganism. In experiment of A and B process for livestock wastewater, we found that the removal efficiency of organic matter and nitrogen in operating mode of A process was higher than that of B process. Also, the optimal F/M operating A process was 0.013 and the removal efficiency of SBOD_5, SCOD_(cr), TN and TP were 97%, 60%, 95% and 91%.

광양만 유역 퇴적토 중의 다환방향족탄화수소류

정흥호,정호승,김은영,조환익,황주찬,최상원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

Polycyclic aromatic hydrocarbons(PAHs) in sediments of Kwangyang bay on Korea, have been investigated by gas chromatography-mass spectrometery (GC/MS). The average content of total PAHs in all samples was 2,211ppb, and the range was from 36 to 22,699pph. The higher concentrations were found in sediment sampled near Kwangyang iron processing plant, Taeindo. The resulting distributions of PAHs according to the number of aromatic ring and molecular ratios of specific aromatic compounds (phenanthrene, anthracene, fluoranthene and pyrene) have been discussed in terms of sample location, origin of the organic matter. Four, five and six rings of PAHs were mainly found near to Kwangyang iron processing plant, and three and four rings near to Yeosu chemical kombinat. This result typically indicated the local characteristics, depending on the origin of PAHs.

천연자원을 이용한 간기능 증진제 개발 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 익모초 추출액의 영향 The Effect of Leonuri Herba on Benzo(a)pyrene Induced Hepatotoxicity

정영건,권오진,윤수홍,박은주 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

Benzo(a)pyrene으로 유도한 rats의 간독성 발현에 미치는 익모초 수침액의 예방 및 치료효과를 실험한 결과 익모초 수침액의 투여는 B(a)P투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성 있게 감소시켰고 B(a)P 투여로 변화한 혈청 지질함량에 약간의 회복도를 보이기는 하나 현저한 차이는 없었다. 즉 익모초의 투여는 B(a)P에 의한 간독성 발현을 효소적으로 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하다. The present study was undertaken to compare the pharmacological activity of crude Leonuri Herba reported with the clinical uses in the oriental medicine. Crude Leonuri Herba used for the treatment of gynecologic condition, irregular menstration, parturition hemaostater and diuretics. Therefore we tested the effects of Leonuri Herba water extract on the liver-protective activities in the rats. The results obtained from liver microsomal enzyme assay, measurement of serum and liver alanine aspartate aminotransferase and lipid accumulation indicated that Leonuri Herba extract showed significant liver-pro-tective activities against benzp(a)pyrene poisoning.

창의력 증진을 위한 과학사적 교수·학습 자료 개발

정원우,이우붕,문장수,이일형,김기정,김은주,손동일,방경곤 경북대학교 사범대학부속 중등교육연구소 2007 중등교육연구 Vol.55 No.1

새로운 알랄아민 항진균제의 합성과 생물학적 평가

정병호,박은주,문현주,유진철 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Some allylamine compounds which are benzothiazole substituants in stead of naphthylring in naftifine, antifungal agents, were sythesized as potential antimycotics. The intermediate Schiff bases that were obtained by condensation of 2-aminobenzothiazole and trans-cinnamaldehyde, were reduced to imine compounds to give allylamines(5a-5d) after mrthylation. These compounds which were tested in vitro against five fungal cell lines containing Trichophyton mentagrophytes. showed no activity in 0.1∼100 μg/ml range.

Lipomyces starkeyi KSM 22 glucanhydrolase 함유 구강세정액의 치태 억제 효과

서은주,정현주,김도만,김상혁 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.3

A novel glucanhydrolase from Lipomyces starkeyi KSM 22 has been suggested as a promising anti-plaque agent because it has been shown to have additional amylase activity and mutanase activity besides dextranase activity and to strongly bind to hydroxyapatite. Mouthrinsing with Lipomyces starkeyi KSM 22 glucanhydrolase solution was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were less frequent and less intense in human experimental gingivitis. In this study, Lipomyces starkeyi KSM 22 glucanhydrolase mouthrinses (1 and 2 unit/ml) were compared with a control mouthrinse (commercial 0.01% benzethonium chloride mouthrinse, Caregargle^(�), Hanmi Pharmaceuticals) in the ability to inhibit on plaque formation. A 3-replicate clinical trial using 4-day plaque regrowth model was used. Fifteen volunteers were rendered plaque-free on the 1st day of each study period, ceased toothcleansing, and rinsed 2X daily with allocated mouthrinse thereafter. On day 5, plaque accumulation was scored and the washout periods was 9 days for the next trial. Lipomyces starkeyi KSM 22 glucanhydrolase(1 unit and 2 unit)- containing mouthrinse resulted in significantly lower plaque formation in plaque area and thickness, compared to the control mouthrinse. There was no significant difference in plaque inhibition between enzyme-mouthrinses at 2 different concentrations used. This glucanhydrolase-containing mouthwash resulted in significantly lower plaque area severity index score and tended to have lower plaque thickness severity index score than those of control mouthrinse. But there was no significant difference according to the enzyme concentration. From these results, Lipomyces starkeyi KSM 22 glucanhydrolase-containing mouthrinse has greater inhibitory effect of plaque formation than commercial mouthrinse alone. Therefore, this glucanhydrolase preparation is a promising agent for new mouthwash formulation in the near future.

광양만으로 유입하는 하천하구에서의 이화학적 특성 : 우수기 직후 유입하천에 따른 영향 effect of inputting rivers after rainy season in summer

김은영,정흥호,정호승,조환익,황주찬,최상원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

Physicochemical characteristics of Kwangyang Bay, have been investigated on the effects of inputting rivers just after rainy season in summer 2003. The results ranged from 4.96~8.01 mg/L for DO, 1.16~3.09 mg/L for COD, 7.21~67.71 μM for DIN and 0.12~121.84 μM for phosphate. The COD of Yeosu Area was 1.19~3.09 mg/L, Kwangyang Area was 1.23 ~ 2.76 mg/L. The COD of Wallae cheon in Yeosu Area was 3.09 mg/L and Donghoan in Kwangyang Area was 2.76 mg/L. The reason of high COD might be industrial sewage of organic chemical plants and iron foundry. DIN of Yeosu Area and Kwangyang Area were 7.21--63.43 μM and 8.43 67.71 μM respectively. The average phosphate of Yeosu Area was 23.65 μM. The source of phosphate might be industrial sewages of fertilizer plant and phosphate gypsum stocked with high amounts. The metal concentrations were measured in the range of 1.01~175.10 ㎍/L for Cu, 0.13~1.78 ㎍/L for Cd, 1.13~5.21 ㎍/L for Pb, 1.58~13.57 ㎍/L for Cr and 1.07~20.68 ㎍/L for As. The average Cu concentration was 78.99 ㎍/L in Kwangyang Area. The source of Cu might be industrial sewage of iron foundry. The average Cr concentration of Yeosu Area was 9.07 ㎍/L. That is 2 times higher than Kwangyang Bay 5.74 gg/L, because of sewage of chemical plants.

치근면에 도포된 Tetracycline의 유리양상에 관한 연구

정은희,정현주 전남대학교 치과대학 1989 전남치대논문집 Vol.1 No.2

This study was performed to observe the desorption pattern of tetracycline-HCI topically applied to root surface. Periodontally diseased human teeth were extracted and root planed thoroughly. Dentin slabs were prepared from roots and applied with 50 ㎎/㎖ and 100 ㎎/㎖ tetracycline-HCI solution for 5 minutes and immersed in 1㎖ of distilled water. The aliquot of distilled water was exchanged and assayed for tetracycline by measuring the absorption peak at 276nm on UV Spectrophotometer every 12 hours. For in vivo study, two individuals diagnosed as moderate to severe periodontitis were selected and 50 ㎎/㎖ and 100 ㎎/㎖ solution of tetracycline-HCI were applied topically to root surfaces during flap surgery. Gingival fluid was sampled from 10 sites per patient using paper strip at 1, 2, 4, 8, 24, 32, 48, 56, 72, 96, 120 and 144 hours. The absorbed paper strips were placed on Mueller-Hinton agar plate containing Bacillus cereus and incubated aerobically in 37℃, 12 hrs and the inhibition diameters were measured. The results were as follows : 1. In vitro study From dentin slabs treated with 50 ㎎/㎖ and 100 ㎎/㎖ tetracycline solution, concentration of tetracycline released in distilled water was 7.32 ㎍/㎖ and 6.18 ㎍/㎖ at 48 hours and 1.3 ㎍/㎖ and 1.42 ㎍/㎖ at 7 days, respectively. The amount of desorption of tetracycline was 5.04 ㎍/㎣ and 5.49 ㎍/㎣ respectively. 2. In vivo study The concentration of tetracycline in gingival fluid was 5.79 ㎍/㎖ and 6.54 ㎍/㎖ at 4 days in 50㎎/㎖ and 100㎎/㎖ tetracycline treated sites respectively. In two sites of 50 ㎎/㎖ tetracycline treated area and three sites of 100 ㎎/㎖ tetracycline treated area, 4 ㎍/㎖ of tetracycline was desorbed still at 6 days. This study indicates that the topical application of tetracycline-HCI results in substantial binding to tooth substance and slow release of effective tetracycline concentration for several days, and the effects of tetracycline may contribute to new attachment of connective tissue.

Bisphosphonate가 배양된 치주인대세포의 조골작용에 미치는 효과

김은영,정현주 전남대학교 치과대학 2001 구강과학 Vol.13 No.1

Previous studies have demonstrated an increase in bone mass density with use of bisphosphonate in osteoporosis. This agent acts as an inhibitor of osteoclastic activity and results in increase of net osteoblastic activity. The purpose of the present study was to examine the effect of the bisphosphonate on osteoblastic activity of the human periodontal ligament cells in vitro. Periodontal ligament cells were primarily obtained from extracted healthy third molars. Cells of 4th to 6th passage were cultured in Dulbecco's modified Eagle's medium containing alendronate sodium or etidronate disodium at the concentration of 10^-12∼10^-16㏖/L in 5% CO_2 incubator at 37℃. Cell count and MTT assay for cellular activity were done at 2 to 7 days of culture. Alkaline phosphatase activity at 4 to 7 days of culture and formation of mineralized nodules at 28 days of culture with addition of 50㎍/㎖ ascorbic acid, 10mM β ­ glycerophosphate, 10^-7M dexamethasone were evaluated. 1. Alendronate sodium Compared to the control, the proliferation of periodontal ligament cells was generally increased and the cellular activity was maintained at 2 days of culture and generally decreased at 7 days of culture. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. 2. Etidronate disodium The proliferation of periodontal ligament cells increased at 2 days of culture and decreased or maintained at 7 days of culture. Compared to the control, the cellular activity of periodontal ligament cells was generally decreased. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. These results suggest that alendronate sodium and etidronate disodium may have a potential effect on osteoblastic lineage of periodontal ligament cells, distinct from their inhibitory action on osteoclasts and could contribute to enhance periodontal regeneration and alveolar bone regeneration.

성견의 외과적 치근이개부 골결손에 차폐막과 골이식재를 이용한 조직유도재생술시 치유양상

정은희,정현주 전남대학교 치과대학 1996 전남치대논문집 Vol.8 No.1

The present study was to evaluate the healing patterns of guided tissue regeneration(GTR) using resorbable Vicry??(polyglactin 910) mesh and nonresorbable expanded polytetrafluoroethylene(ePTFE) membrane with or without bone grafting using autogeneous bone and demineralized freeze-dried bone allograft(DFDBA) in the grade Ⅱ furcation defects. Mucoperiosteal flaps were reflected buccally in the mandibular 2nd, 3rd and 4th premolar areas and furcation defects were created surgically by removing 5 × 6mm alveolar bone in 4 dogs. Root surfaces were thoroughly debrided of periodontal ligament and cementum, and notches were placed on root sruface at the most apical bone level. In the right and left mandibular quadrant, each tooth was received Vicryl?? mesh(ACE Surgical Supply Co., USA) only, Vicryl?? mesh with DFDBA, Vicryl?? mesh with autogeneous bone grafts, ePTFE membrane(Gore-tex?? membrane, W.L. Gore & Associates Inc., USA) only, ePTFE membrane with DFDBA or ePTFE membrane with autogeneous bone grafts. For the fluorescent microscopic examination, fluorescent agents were injected at 2, 4 and 8 weeks after surgery. Four weeks after surgery, 2 dogs were sacrificed and ePTFE membranes were removed from remaining 2 dogs, which were sacrificed at 12 weeks after surgery. Undecalcified tissues were embedded in cethylmethacrylate and 10㎛ thick sections were cut in a buccolingual direction. These sections were stained with hematoxylin-eosin stain and Masson's trichrome stain, and evaluated by descriptive histology and linear measurements. The results were as follows : 1) Vicryl?? mesh group showed less connective tissue attachment than ePTFE membrane group. 2) The combination of GTR using Vicryl?? mesh and osseous grafts resulted in new attachment and new bone formation more than GTR using Vicryl?? mesh only. 3) GTR using ePTFE membrane, with or without osseous grafts, enhanced periodontal regeneration. 4) Root resorption and dentoalveolar ankylosis were observed in the areas treated with the combination of GTR and DFDBA. It was suggested that the effect of adjunctive bone grafting in GTR procedure depends on the materials and the physical properties of barrier membranes. Vicryl?? mesh performed a barrier function and the use of adjunctive bone grafting may enhance the periodontal regeneration.