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Boh Kyung Kim,Ji Myung Choi,Soon Ah Kang,Kun Young Park,Eun Ju Cho 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.6
BACKGROUND/OBJECTIVES: Kimchi is a traditional Korean fermented vegetable containing several ingredients. We investigated the protective activity of methanol extract of kimchi under different fermentation stages against oxidative damage. MATERIALS/METHODS: Fresh kimchi (Fresh), optimally ripened kimchi (OptR), and over ripened kimchi (OvR) were fermented until the pH reached pH 5.6, pH 4.3, and pH 3.8, respectively. The radical scavenging activity and protective activity from oxidative stress of kimchi during fermentation were investigated under in vitro and cellular systems using LLC-PK1 cells. RESULTS: Kimchi exhibited strong radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, superoxide anion, and hydroxyl radical. In addition, the free radical generators led to loss of cell viability and elevated lipid peroxidation, while treatment with kimchi resulted in significantly increased cell viability and decreased lipid peroxidation. Furthermore, the protective effect against oxidative stress was related to regulation of cyclooxygenase-2, inducible nitric oxide synthase, nuclear factor-κB p65, and IκB expression. In particular, OvR showed the strongest protective effect from cellular oxidative stress among other kimchi. CONCLUSION: The current study indicated that kimchi, particularly OptR and OvR, played a protective role against free radical-induced oxidative stress. These findings suggest that kimchi is a promising functional food with an antioxidative effect and fermentation of kimchi led to elevation of antioxidative activity.
배추의 항돌연변이 및 in Vitro 항암 효과 -터널식 재배 방법에 의한 배추의 기능성 증진-
전혜경 ( Hye Kyung Chun ),김보경 ( Boh Kyung Kim ),박홍주 ( Hong Ju Park ),조수목 ( Soo Muk Cho ),김세나 ( Se Na Kim ),곽정호 ( Jung Ho Kwak ),박건영 ( Kun Young Park ) 부산대학교 김치연구소 2009 김치의 과학과 기술 Vol.12 No.-
The antimutagenic and anticancer effects of baechu cabbage (Brassica campestris L. ssp pekinensis [Lour.] Rupr.) cultivated in Noji and Tunnel in spring were investigated under in vitro and cellular system using HT-29 human colon carcinoma cells. Proximate composition, mineral and vitamin contents of baechu cabbages (Noji and Tunnel) were evaluated. Antimutagenicity were measured in Ames test using N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) and aflatoxin B1 (AFB1). The viability and growth of HT-29 cells were evaluated by MTT (3-(4,5-dimethyl-thiazol)-2,5-diphnyltetrazolium bromide) assay and growth inhibition test, respectively. The gene and protein expressions of apoptosis-related proteins such as Bax, Bcl-2, p53 and p21 were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and western blot. The contents of carotene, Ca, K in Tunnel baechu cabbage (Tunnel) were higher than those of Noji baechu cabbage (Noji). The methanol extract from the spring baechu cabbage particular cultivated by Tunnel method effectively reduced mutagenicity induced by MNNG. The Tunnel also showed higher antimutagenic effects against AFB1 than the Noji. The HT-29 cells showed the significant decreases in cell viability by the treatment of the Tunnel with dose-dependence in MTT assay. The Tunnel also inhibited on the growth of HT-29 cells in a time-dependent manner. The baechu cabbage induced apoptosis in HT-29 cells were associated with the decreased expression of the anti-apoptotic Bcl-2 and increased expression of p53, p21 and Bax. The present study suggests that the baechu especially the Tunnel would have increased antimutagenic effect and anticancer activity through induction or repression of apoptosis. (Cancer Prev Res 13, 122-129, 2008)
천연해수에 침지된 콘크리트 내부의 철근부식거동에 미치는 염분의 영향에 관한 전기화학적 연구
김광근(KWANG-KUEN KIM),류보현(BOH-HYUN RYU),김성종(SEONG JONG KIM),김기준(KI-JOON KIM),문경만(KYUNG MAN MOON) 한국해양공학회 2000 韓國海洋工學會誌 Vol.14 No.4
When the concrete structures were being made with sand containing chloride ion it was well known that corrosion rate of reinforced steel embedded in concrete with chloride ion was higher than that of concrete with no chloride ion. In this study, the operation of Friedel salts affecting the corrosion behavior of reinforced steel embedded in cement mortar was investigated with electrochemical view. Corrosion potential of reinforced steel embedded in cement mortar with sand containing chloride ion was shifted noble direction than that of cement mortor with no chloride ion after immersed 5 month in natural sea water and also corrosion current density decreased with shifting corrosion potential to noble direction. However Friedel salts appeared from surface to 2.5㎝ of inside direction of mortar specimen, which is located at 11.15˚(2 θ) in XRD analysis and the amount of Ca(OH)2 by SEM photograph in cement mortar with chloride ion was larger than that of cement mortar with no chloride ion. Eventually it is suggested that Friedel salts was resulted from chloride ion and it acted as the corrosion inhibitor.
Ui Nam Ryoo,Chel Hun Choi,Ji Yeong Yoon,Soo Kyung Noh,Heeseok Kang,Woo Young Kim,Boh Hyun Kim,Tae-Joong Kim,Jeong-Won Lee,Je-Ho Lee,Byoung-Gie Kim,Duk-Soo Bae 대한암학회 2007 Cancer Research and Treatment Vol.39 No.4
Purpose: The purpose of this study was to evaluate the factors that are associated with the accuracy of magnetic resonance (MR) imaging for predicting myometrial invasion and lymph node metastasis in women with endometrial carcinoma.Materials and Methods: We retrospectively reviewed the medical records and preoperative MR imaging reports of 128 women who had pathologically proven endometrial carcinoma. We compared the MR imaging and the histopathology findings.Results: The sensitivity, specificity and accuracy for identifing any myometrial invasion (superficial or deep) were 0.81, 0.61 and 0.74, respectively; these values for deep myometrial invasion were 0.60, 0.94 and 0.86, respectively. The sensitivity, specificity and accuracy of MR imaging for detecting lymph node metastasis were 50.0%, 96.6% and 93.0%, respectively. The patients who were older, had more deliveries and a larger tumor size more frequently had incorrect prediction of deep myometrial invasion (p=0.034, p=0.044, p=0.061, respectively). A higher tumor grade, a histology other than the endometrioid type, myometrial invasion on MR findings and a larger tumor size were associated with a more frequent false-negative prediction of lymph node metastasis (p=0.018, p=0.017, p=0.002, p=0.047, respectively). A larger tumor size was also associated with more frequent false-positive results (p=0.009).Conclusions:There are several factors that make accurate assessment of myometrial invasion or lymph node metastasis difficult with using MRI; therefore, the patients with these factors should have their MR findings cautiously interpreted. (Cancer Res Treat. 2007;39:165-170)
Antimutagenic and Anticancer Effects of Leaf Mustard and Leaf Mustard Kimchi
Kim, Yong-Taek,Kim, Boh-Kyung,Park, Kun-Young The Korean Society of Food Science and Nutrition 2007 Preventive Nutrition and Food Science Vol.12 No.2
In this study, we investigated antimutagenic and anticancer activities of leaf mustard (LM, Brassica juncea) and leaf mustard kimchi (LMK) during their fermentation period. Methanol extracts were prepared from raw mustard, brined leaf mustard in 10% Gueun salt solution for 2 hrs, leaf mustard fermented at 15$^{\circ}C$ for 5 days after brined in 10% Guenun salt solution for 2 hrs (Fr-LM), fresh leaf mustard kimchi (Fresh-LMK) and optimally ripened leaf mustard kimchi fermented at 5$^{\circ}C$ for 30 days (OR-LMK). OR-LMK showed the strongest inhibitory activities against the mutagenicities induced by aflatoxin B1 in Salmonella Typhimurium TA100. LMs and LMKs inhibited the survival or growth of AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells in MTT assay and growth inhibition test. Among the extracts, OR-LMK and FR-LM exhibited strong antiproliferative effect against cancer cells, especially HT-29 cells. DAPI staining assay showed that OR-LMK induced apoptosis cell death of HT-29 cells in a dose-dependent manner. These results suggest that leaf mustards and leaf mustard kimchi have chemopreventive activities.
Hong, Kyung-Won,Kim, Sang-Wook,Jang, Hong-Chul,Yang, Seung-Min,Shin, Young-Bin,Hong, Yoon-Hye,Kim, Jong-Seok,Oh, Seok-Il,Choi, Yoon-Ju,Chung, Dong-Hee,Yang, Boh-Suk,Lee, Ji-Woong,Choi, Bong-Hwan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8
The Jindo dog is a Korean natural monument and is recognized by the Fédération Cynologique Internationale. A prominent feature is the diverse coat color within the breed. To analyze the genetic basis of variation in the Jindo coat color, we sequenced the protein-coding regions of the melanocortin 1 receptor gene (MC1R). The MC1R coding sequence was determined from 154 dogs in five breeds (Jindo, Labrador Retriever, English Springer Spaniel, Belgian Malinois, and German Shepherd). To confirm the genetic structure of sampled populations, we tested for Hardy-Weinberg equilibrium (HWE) and computed $F_{st}$ The sample populations did not significantly deviate from HWE. $F_{st}$ was 0.02 between white and fawn Jindo dogs; this was lower than $F_{st}$ between breeds. Six single nucleotide polymorphisms (SNPs) were detected in the MC1R coding region. Among the six SNPs, five were non-synonymous (S90G, T105A, Q159P, M264V, and R306ter) and one was synonymous SNP (Y298Y). From the SNPs, we predicted four haplotypes (H1, H2, H3, and H4) for Jindo MC1R. Jindo dogs had different haplotypes corresponding to different coat colors. H1 was frequently observed in white Jindo dogs with an odds ratio of 5.03 (95% CI: 2.27-11.18, p<0.0001), whereas H2 and H4 were observed only in fawn Jindo dogs. Our findings indicate that SNP haplotype can influence coat color. Knowledge of MC1R haplotypes can help discriminate white and fawn coats in Jindo dogs. We hope this report will trigger more research into the genetics of this traditional Korean dog and will be a reference for dogs of Asian origin. Also, our results will provide a useful genetic marker for Jindo dog breeders who have selected for specific colors.