Oil Contents and Fatty Acid Composition of Korean Perilla (Perilla ocimoides L.) Collections

Hong,Seong-Taek,Son,Suk-Yeong,Jong,Seung-keun,Rho,Chang-Woo,Yun,Jong-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3

This study was carried out to obtain informations on the variations of oil content and fatty acid composition among 90 Korean perilla collections. Average oil content of 90 perilla collections was 44.2% with a range from 29.7% to 61.9%. Perilla collections with late-maturing, super-large seed and gray seed coat showed higher oil content than other types in general. Average saturated fatty acid content in perilla oil was 9.0% with a range from 8.2% to 10.7%, while average unsaturated fatty acid content varied from 89.3% to 91.8% with a mean of 91.0%. Contents of saturated and unsaturated fatty acids were not related to maturity. There were no differences in the contents of saturated and unsaturated fatty acids according to maturity. Linoleic acid and linolenic acid contents were the highest in the super large-sized group(15.5%) and in the large-sized seed group(61.4%), respectively, while contents of fatty acids among the perilla collections were variable with different seed coat colors. Most of the traits studied were not significantly correlated with oil content, but linoleic acid($\omega$ 수식 이미지-6) content was negatively correlated (r=-0.217*) with linolenic acid(ω-3) content.

인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

이동호스트를 위한 적응성 멀티캐스트 알고리즘

홍영표,안광수,백덕화 진주여자전문대학 2001 論文集 Vol.24 No.-

To Provide multicast service for mobile hosts must deal with dynamic member location and its group membership in mobile networks. There are two old approaches as hi -directional tunneling and remote subscription for mobile multicast. The deployment of existing multicast protocols(home agent-based multicast, Bidirectional packet tunneling) is very inefficiently to use network resources such as duplicate copies of multicast datagrams, tunnel convergence, and unicast by long delivery path. The remote subscription scheme may be very expensive because muticast packet delivering is delayed and discontinued frequently by rebuilding a multicast tree to serve multicast when the host move highly. In this paper, we analyse these problems of multicast routing protocols and propose an efficient multicast protocol using cost function to consider the host mobility in wireless mobile networks, By cost function, a mobile host make a decision to receive a tunneled multicast datagram from home agent or to receive a rebuild path from foreign agent. The performance of the proposed protocol was evaluated by simulation under various environments and we got an improved performance.

콩의 식품적 의의 및 생산수급과 식용콩의 자급 향상

이홍석,이영호,이석하 대한민국 학술원 2011 학술원논문집 : 자연과학편 Vol.50 No.2

콩은 단백질 공급을 비롯하여 영양가가 높아 예로부터 우리의 중요 식품으로 널리 다양하게 이용하여 왔을 뿐 아니라 지속적인 밭작물 재배를 가능케 하는 중요한 역할을 하여 왔으며, 최근에는 건강식품으로 또 친환경 농업 작물로 주목을 받고 있다. 콩은 세계적으로 99,501천ha에서 223,185천톤(2,243kg/ha)이 생산되고 그의 35.5% (35,130백만불)가 수출되고 그 중 43%는 미국 콩이, 96.5%는 미대륙 생산 콩이 수출되고 있으며, 수입의 반 정도는 중국이, 37.6% 정도는 유럽의 선진국과 일본 등 10여 개국에서 각각 수입하고 있고, 우리나라도 10번째의 수입국이며 세계적 소비수요는 계속 증가되고 있다. 우리나라는 1968년의 313,501ha의 재배에서 계속 감소하여 현재는 75,242ha에서 132,674톤이 생산되고 1,261천톤이 수입되었으며, 전체 자급률은 수요량 1,333천톤의 8.6%, 식용 및 기타는 수요량 387천톤의 29.5%에 불과하다. 콩, 특히 식용콩의 증산에 의한 자급율 향상은 경지정리 및 주산단지 조성을 통한 생력기계화 재배를 전제로 첫째, 밭의 작부체계 개선 둘째, 논의 밭 전환재배 셋째, 북한의 동해안 지대의 밭을 장기임대하여 재배하고 그 대가를 쌀 지원으로 하는 것이며 넷째는 인접국에서 밭을 매입 또는 장기 임대하여 생산 공급하는 방법 등을 생각할 수 있을 것이다.

생물계면활성제를 이용한 중금속 및 유기물 오염 토양의 정화

홍석정,현재혁,김영철 한국공업화학회 2002 응용화학 Vol.6 No.1

A study on the soil remediation was performed by using a biosurfactant. The surfactant solutions were applied to the soil flushing of the artificially contaminated soil with copper ion, cadmium ion and phenol. The optimal concentration of the surfactant was determined by the lab.-scale column tests using the solutions of various concentrations. The characteristic behavior of the contaminant removal was studied and the efficiencies of the soil flushing were determined for the contaminants.

외래어종 베스, Micropterus salmoides를 포함하는 군집의 종간 Association에 관한 연구

홍영표,손영목 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.1

1970년부터 2002년까지 전국을 조사한 자료 중 외래도입어중인 M. salmoides가 포함된 군집을 대상으로 공존성 및 종간상관성에 대하여 논의하였다. 총 7,192개의 조사지역에서 M. salmoides가 포함된 군집은 46군집, 공존하는 어종은 72종으로 나타났다. 공존성이 높은 종은 Z. platypus, O, bidens, H. eigenmanin, S. japonicus coreanus의 순이었다. 종집단간 공존도는 M. salmoides - Z. platypus 집단, M. salmoides - C. auratus 집단, M. salmoides 집단을 포함하고 있는 군집내에서 7종이 그룹화되었다. M. salmoides가 포함된 군집내에서 O. bidens와 Z. platypuswlqeks 등 7개 종집단은 종간 상관성이 매우 높았으며(p<0.01), L. macrochirus와 C. cuvieri 집단 등 11개 종집단은 종상관성이 다소 높은 것으로 나타났다(p<0.05). 한국의 하천이 점차 호수성하천으로 변화된다고 볼 때, M. salmoides 집단의 조절대책이 필요하다. On the fish community including Micropterus salmoides population, introduced species in Korea, interspecific association through coexistence by presence-absence among species populations were discussed from the year of 1970 to 2002. In the total of 7,192 sampling stations in Korea, the community including Micropterus salmoides population were confirmed at 46 sampling sites, and coexistent fishes were 72 species. Fish species having high coexistence rate were Zacco platypus, Obsarlichthys bidens, Hemiculter eigenmanni and Squalidus japonicus coreanus populations in the order of their number. A association indices values between population were in the order of Micropterus salmoides-Zacco platypus, Micropterus salmoides-Carassius auratus, and Micropterus salmoides-Hemiculter eigenmanni population. Judging from the dendrogram according to association indices between species population, 7 species populations were clustered in the community including M. salmoides population. Being based on association values among species population in the community including M. salmoides population, 11 species population pairs including Lepomis macrochirus -Carassus cubieri etc. showed somewhat high significance (p < 0.05), and 7 species population pair including Zacco platypus-Obsarlichthys bidens etc. represented higher association among species population (p < 0.01). With the increasing of lake- river in Korea, it keenly need to take a regulation program of the Micropterus salmoides population.

超微量 銅의 螢光光度定量法

洪永大 고신대학교 자연과학연구소 1992 고신대학교 자연과학연구소 논문집 Vol.2 No.-

A spectrofluorometric method for the ultra microdetermination of copper was established. An excess amount of 2-(o-hydroxypheny1)benzoxazole(HPB) is added to precipitate cu(HPB)₂ with copper ion. Copper ion forms a chelate with HPB and quenches the fluorescence of HPB. The amount of copper is determined by measuring the fluorescence of the remaining HPB. The optimum conditions for these reactions were examined at a very low concentration range of the reactants. By the proposed method, 0.025~5 nmol copper content was determined with a accuracy of 2.1_(7)% relative standard deviation at 2 nmol Cu level.

超微量 亞鉛의 螢光光度定量法

洪永大,李正禹 고신대학교 자연과학연구소 1997 고신대학교 자연과학연구소 논문집 Vol.7 No.-

超微量의 Zn을 定量하기 위하여 2-(o-hydroxyphenyl)benzoxazole (HPB)을 利用하는 高感度의 螢光分析法이 確立되었다. 0.65㎍ 以下의 Zn(Ⅱ) 이온을 含有하는 試料溶液에 이것에 比하여 一定過量의 HPB 溶液을 加하여 約 pH 9.4에서 反應시키면 螢光이 없고 安定度가 큰 Zn(HPB)_2 의 킬레이트 化合物을 만들어 定量的으로 沈澱한다. 그런 다음, 溶液 中에 남아 있는 HPB의 螢光强度를 測定함으로써 Zn(Ⅱ) 이온의 濃度를 間接 測定하였다. 本 硏究에서는 nmol level의 超微量 濃度에서 Zn(Ⅱ) 이온과 HPB의 反應이 化學量論的으로 일어 나는데 必要한 最適條件들을 綿密히 檢討한 結果, 0.5-10 nmol의 Zn을 感度좋게 定量할 수 있었다. 本 定量法을 使用하여 5 nmol의 Zn을 反復 測定하여 본 結果, 相對標準偏差 2.1%의 精密度로서 測定할 수 있었다. A spectrofluorometric method for the ultra microdetermination of zinc was established. An excess amount of 2- (o-hydroxt'phenyl)benzoxazole(HPB) is added to precipitate Zn(HPB)₂ with zinc ion. Zinc ion forms a chelate with HPB and quenches the fluorescence of HPB. The amount of zinc is determined by measuring the fluorescence of the remaining HPB. The optimum conditions for these reactions were examined at a very low concentration range of the reactants. By the proposed method, 0.5-10 nmol zinc content was determined with a accuracy of 2.1% relative standard deviation at 5 nmol Zn level.

웹기반 저작도구 시스템 설계 및 구현

홍영표 진주여자전문대학 2000 論文集 Vol.23 No.-

To contract cycle education on the web, the instructor should be able to use web-related tools. And the contents so far have fixed screen composition and menu, which made the learner bored at class because they have to use same screen composition in each subject. This study makes the instructor conduct cycle education even if he can not use web tools. The instructor can compose various contents easily only by choosing an clicking menu for his class. He can also add functions by connecting what he whats to URL. For the learners, m will be composed of menu required only for the chosen class and screen composition will be different for each subject. This learners will have better studying environment because they have different screen composition for each subjects.

수면 부유 방법에 의한 연초 육묘

洪淳達,石泳善,朴孝澤 충북대학교 엽연초연구소 1999 煙草硏究 Vol.17 No.-

Carbonized rice hull, perlite, and vermiculite were mixed and investigated for the probability of tobacco transplant production by means of plug and transfer float system. These seedling bed materials in condition of float system have gaseous phase ranged from 6% to 13% showing a proper growth of tobacco seedling. However compost media mixed with soil, sand, and compost in condition of float system led to the shortage of respiration in root system by very low proportion of gaseous phase skewing a wicked growth of seedling. Absorption of heat by floating water for the daytime increased rhizosphere temperature in condition of float system during the night and low temperature season resulting in better growth of tobacco seedling. Consequently transplant production of tobacco seedling by float system with mixed materials of carbonized rice hull, perlite, and vermiculite was thought to be desirable method for manufacturing production system of transplant considering the effective management of water, nutrients, and temperature of seedling bed materials.